• 한국응용과학기술학회지
• /
• 제31권4호
• /
• pp.653-662
• /
• 2014

This study was determined the hair growth effect and mode of action of a complex extract (abelmo), extracted from various natural plants including Acorus calamus var. angustatus. 30 six-week old C57BL/6 mice were randomly assigned into three treatment groups with ten mice per treatment group repeatedly. The treatment group is a control group, and was classified into saline-applied, minoxidil 5%-applied, and abelmo-applied groups. Hair growth started from the abelmo-applied and minoxidil 5% -applied groups on the 9th day, compared to the saline-applied group. Hair growth rate was 17.06% and 19.15% in those two groups, respectively, and the abelmo-applied group's hair growth rate was higher. The density, length and thickness of hair were significantly higher in the order of abelmo-applied group, minoxidil 5%-applied group and saline-applied group on the 9th day, and hair root was strongly maintained in the abelmo-applied group. The density, length and thickness of hair in the abelmo-applied group were significantly higher by 599.8, 122.2 and 181.8% on the 9th day compared to the saline-applied group, and 166.7, 171.4 and 200.2% on the 12th day, and 136.6, 216.9 and 180.7%, respectively on the 15th day. As for hair follicle and the length of hair shaft from the dosal skin histopathology manifestation, the abelmo-applied group was more excellent than the saline-applied group and minoxidil 5%-applied group. This result actually confirmed the fact that abelmo promotes hair growth and strongly maintains hair root simultaneously.

• 농업과학연구
• /
• 제41권1호
• /
• pp.47-58
• /
• 2014

The effect of conjugated linoleic acid (CLA) isomers esterified in diacylglycerol (DAG)-rich oil on lipid metabolism was investigated. Since dietary DAG has been known to induce the regression of atherosclerosis, CLA-DAG and olive-DAG oils containing similar levels of DAG (51.4~54.2%) were synthesized from olive oil. Hyperlipidemic C57BL/6J mice were then fed high-fat high-cholesterol diets supplemented with these oils (5% each) for 7 wk. The CLA-DAG diet containing 2.1% CLA isomers (0.78% c9,t11-CLA; 1.18% t10,c12-CLA) remarkably increased the levels of total plasma cholesterol and glutamic oxaloacetic transaminase (GOT) along with hepatic cholesterol and triacylglycerol (TAG) contents. Furthermore, the CLA-DAG diet inhibited fat uptake into adipose tissue whereas fat deposition (especially in the liver) was increased, resulting in the development of fatty livers. Hepatic fatty acid composition in the CLA-DAG mice was different from that of the olive-DAG mice, showing higher ratios of C16:1/C16:0 and C18:1/C18:0 in the liver. The activity of hepatic acyl-CoA:cholesterol acyltransferase (ACAT) was higher in CLA-DAG mice while plasma lecithin:cholesterol acyltransferase (LCAT) activity and the ferric reducing ability of plasma (FRAP) were lower in CLA-DAG mice compared to the olive-DAG animals. Results of the present study suggest that CLA incorporation into DAG oil could induce atherosclerosis in mice.

• Toxicological Research
• /
• 제18권2호
• /
• pp.205-213
• /
• 2002

This study was undertaken to develop a subacute murine model for predicting occurrence of systemic anaphylaxis and to evaluate efficacy of various immunological parameters as the monitoring indices for the occurrence of anaphyalxis. The murine anaphyalxis model was developed through intraperitoneally sensitizing 100 $\mu\textrm{g}$ ovalbumin (OVA) in the presence of 1 mg alum and 300 ng cholera toxin twice a week interval followed by challenging 500 $\mu\textrm{g}$. OVA intravenously. Typical anaphylaxis symptoms were demonstrated at the both BALB/c mice, a strain prone to type-2 response, and C57BL/6 mice. a strain prone to type-1 response. Level of plasma histamine was approximately 50-fold or 30-fold higher in the mice sensitized with OVA than the mice sensitized with alum plus cholera toxin or the saline-treated mice after OVA challenge, respectively. Sensitization and challenge with OVA significantly enhanced plasma leukotriene $B_4$ level but not IgE levels in comparison with the control mice, which indicated the role of leukotriene $B_4$ for progression of anaphyalxis. Furthermore, among mice suffered from anaphylaxis, levels of OVA-specific IgGl were significantly higher in the BALB/c mice than in the C57BL/6 mice, which implied the genetic susceptibility for the induction of systemic anaphylaxis. Conclusively, simultaneous evaluation of histamine, leukotriene $B_4$, and allergen-specific IgG isotype may serve as more efficient monitoring tool for vaccine-related anaphyalxis.

• Journal of Applied Biological Chemistry
• /
• 제65권4호
• /
• pp.313-319
• /
• 2022

비만의 원인이 되는 고지방식이 섭취는 체내에서 과다한 지질 축적 및 염증 반응을 유도한다. 본 연구에서 고지방식이로 비만이 유도된 C57BL/6 동물 모델에서 흑양파식초 섭취가 미치는 효과에 대해 알아보기 위해 흑양파즙 및 흑양파식초를 각각 6주간 투여하였다. 고지방식이를 유도한 HFD군은 정상 식이를 섭취한 NC군에 비해 체중 및 장기 무게가 증가하여 비만이 유도되었음을 확인하였다. 반면 흑양파식초를 투여한 HFD + BV군은 HFD군에 비해 체중 및 장기 무게를 유의적으로 감소시켰다. 또한 흑양파즙 및 흑양파식초를 각각 투여한 군은 HFD군에 비해 혈청 총 콜레스테롤 및 중성지질 수치를 개선시켰다. 뿐만 아니라, 흑양파식초 투여군은 HDL 콜레스테롤 및 LDL 콜레스테롤 수치를 유의적으로 개선시켜 고지방식이로 손상된 지질대사 개선 효과를 확인하였으며, 부고환 지방 및 간 조직에서 지방세포의 수와 크기를 감소시켰다. 흑양파식초의 비만개선 작용 기전을 확인하기위해 간 조직 내에서 adipogenesis 및 염증 관련 단백질 발현 측정 결과, 흑양파즙 및 흑양파식초 투여군은 HFD군에 비해 adipogenesis 및 염증 관련 인자를 감소시켰다. 특히 흑양파식초 투여는 흑양파즙 투여에 비해 지방생성에 관여하는 단백질 억제 효과가 더욱 우수하였다. 따라서 본 연구에서 흑양파식초 투여는 고지방식이로 비만이 유도된 동물모델에서 adipogenesis 관련 단백질 발현 억제를 통해 비만을 개선시킴을 알 수 있었으며, 항비만 기능성 식초로 활용 가능성이 있는 것으로 사료된다.

• 동아시아식생활학회지
• /
• 제16권4호
• /
• pp.414-420
• /
• 2006

The present study evaluated the effects of pomegranate peel (Granati pericarpium) extract on the lipid profiles and antioxidant biomarkers in mice fed a high fat and cholesterol diet: the measured biomarkers included the TBARS value, GPx, GR, SOD and GST activities. Body fat depositions were significantly decreased in the group that received pomegranate peel. In addition, the activities of GPx, GST and SOD were significantly higher in the liver and plasma of the pomegranate peel group than in the control group. Also, the pomegranate peel diet decreased lipid peroxidation of the liver and kidney. Alkaline single-cell gel electrophoresis (comet assay) showed that the DNA damage in the plasma of the pomegranate peel group was decreased compared to that of control. The present results show that a diet with added pomegranate peel exerts protective effects against oxidative DNA damage and lipid peroxidation possibly via effects on the free radical levels in mice fed a high fat and cholesterol diet.

• 대한의생명과학회지
• /
• 제11권1호
• /
• pp.1-8
• /
• 2005

Fibrates are a class of hypolipidemic agents whose effects are mediated by activation of a specific transcription factor called the peroxisome proliferator-activated receptor $\alpha\;(PPAR\alpha).\;PPAR\alpha$ regulates the pathways of lipid catabolism such as fatty acid oxidation and the triglyceride metabolism, resulting in the treatment of hyperlipidemia. The decreased levels of plasma triglycerides by fibrates are responsible for hypertrophy and hyperpalsia of adipose cells. To determine whether fenofibrate regulates adipogenesis in female C57BL/6J mice, we measured the effects of fenofibrate on not only body weight, adipose tissue mass and serum triglycerides, but also the histology of adipose tissue and the expression of adipocyte marker genes. Fenofibrate did not inhibit high fat diet-induced increases in body weight, adipose tissue mass and serum triglycerides. Furthermore, fenofibrate did not cause the changes in the size and number of adipocytes and the expression of adipocyte-specific genes such as leptin and $TNF\alpha$. Therefore, this study demonstrates that fenofibrate does not affect adipogenesis in female mice.

• 대한의생명과학회지
• /
• 제12권4호
• /
• pp.319-327
• /
• 2006

This study aimed to determine whether troglitazone stimulates genes related to fatty acid ${\beta}$-oxidation, leading to modulation of white adipose tissue (WAT) metabolism in high fat diet-fed mice. Female C57BL/6J mice were randomly divided into two groups (n=10/group). After they received either a high fat diet or the same high fat diet supplemented with troglitazone for 4 weeks, the effects of troglitazone on gene expression and physiology of WAT were measured using Northern, histological and serological analyses. Administration of troglitazone induced the expression of genes involved in mitochondrial and peroxisomal fatty acid ${\beta}$-oxidation in mesenteric WAT. Troglitazone also significantly increased uncoupling protein 2 mRNA levels. The changes in WAT gene expression were accompanied by reductions in circulating levels of free fatty acids and triglycerides as well as glucose and insulin. Histological studies showed that troglitazone treatment decreased the average size of adipocytes in mesenteric WAT. These results suggest that troglitazone-stimulated WAT expression of genes associated with fatty acid ${\beta}$-oxidation regulates WAT metabolism of high fat diet-fed mice, contributing to improvement of insulin sensitivity.

• 한국기계가공학회지
• /
• 제15권4호
• /
• pp.30-39
• /
• 2016

There have been many cases of deaths from crushing caused by dense crowds. Numerous studies about pedestrian flow have performed various simulations, but the experimental data to prove the simulations are still not enough. In this paper, the evacuation of pedestrians for proving pedestrian flow simulation is observed. Due to the possibility of real casualties, it is difficult to experiment with humans directly. Therefore, ten C57BL/6NCrSIc mice have been used. It is assumed that C57BL/6NCrSIc mice act like humans in panic situations. Electrical Stimulus Experiments on mice are conducted for exits with various angles. ICY software is applied in this paper. As a result, the mice escape fast at a proper angle of 45 to 60 degrees.

• Journal of Applied Biological Chemistry
• /
• 제55권1호
• /
• pp.1-5
• /
• 2012

Recently, we reported that Jeju ground water contains vanadium components and exerts antioxidant effects in vitro and in vivo via the scavenging of reactive oxygen species and enhancement of antioxidant enzyme activities. In the present study, the antioxidant actions of Jeju ground water were compared with those of tap water against gamma-ray radiation in mice. C57BL/6 mice were irradiated with gamma-ray at a dose rate of 2 Gy. The mice were then given tap water or Jeju ground water for 90 days. Jeju ground water compared with tap water enhanced the activities and levels of superoxide dismutase, catalase, and glutathione peroxidase in irradiated liver tissues. Jeju ground water also enhanced the levels of intracellular reduced glutathione, which is vital for normal liver function and repair. These results suggest that vanadium-containing Jeju ground water can safeguard against the harmful actions of gamma-ray radiation through the support of hepatic antioxidant processes.

• 대한한방부인과학회지
• /
• 제21권4호
• /
• pp.1-16
• /
• 2008

Purpose: The purpose of this study is to investigate the effect of Boheo-tang (B) and Boheo-tang plus cervi pantotrichum cornu (B+CP) on lactation in postpartum C57BL/6N mice. Methods: Normal saline(control), Band B+CP (8mu l/g$) were administerd p.o. twice a day for 20 days. Lactating mammary gland tissues were examined through light microscope by the way of HE staining and immunohistochemical assay. Milk producing associated gene expression were accessed by RT-PCR. Results: In mammary gland, amount of adipose tissues were decreased in both Band B+CP treated group. And the ductal branches and alveolar tissues increased in both treated group. Immunoreactivity of prolactin receptors was increased both treated group, and immunoreactivity of oxytocin receptors was increased in the B+CP treated group. In both treated group, IGF-l mRNA expression was increased and TGF-$\beta$ mRNA expression was decreased. And PRL mRNA expression was increased in the B+CP treated group. PL-l mRNA expression was decreased in the B treated group but increased in the B+CP treated group. Conclusion: This study shows that treatment of Boheo-tang and Boheo-tang plus cervi pantotrichum cornu can improve postpartum lactation in C57BL/6N mice.