• Title/Summary/Keyword: C5 Protein

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Studies on the Chemical Compositions and Quality of Red Pepper Paste Brewed with Different Raw Materials (담금원료(原料)에 따른 고추장의 성분(成分)과 품질(品質)에 관(關)한 연구(硏究))

  • Park, Soo-Woong;Park, Yoon-Joong
    • Korean Journal of Agricultural Science
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    • v.6 no.2
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    • pp.205-212
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    • 1979
  • Red pepper paste were prepared by using various raw materials such as rice, glutinous rice, polished barley, polished wheat and corn powder, and their chemical compositions including reducing sugar, amino-N and ethanol were analyzed during the period of aging in order to elucidate effects of these starchy materials on the compositions and qualities of the products. The results obtained are summarized as follows: 1) Koji of these materials were manufactured respectively by inoculating a strain of Asporyzae and their activities of protease and saccharifying amylase were determined. Wheat koji was found to have the highest level of protease activity among the koji, while glutinous rice koji had the strongest amylase activity. 2) Contents of moisture, crude protein, crude fat, and sodium chloride in the red pepper pastes were not changed significantly, however total sugar content was decreased during the period of aging. 3) After 60 days of aging, the highest amount (160mg%) amino nitrogen was detected in the red pepper paste of polished wheat, but higher levels of reducing sugar and ethanol contents were detected in that of glutinous rice. 4) Amino acids in the products were analyzed after 60 days of aging: a) Total 17 amino acids were detected. b) Free amino acid ratio to total amino acid content was approximately 35 percent in average. c) Free glutamic acid was higher than any other free amino acid In the amount and free ratio, but free methionine was lower. d) Lysine content was relatively high, while histidine and glycine were detected in trace. 5) Sensory tests including color, odor and taste on the products resulted in the highest mark for the red pepper paste of glutinous rice but in the lowest mark for that of corn powder.

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Effects of Endotoxin and Verapamil on Superoxide Production by Rat Alveolar Macrophage (백서폐포대식세포에서의 Superoxide 생산에 미치는 내독소 및 Verapamil의 영향)

  • Lee, Choon-Taek;Kim, Keun-Youl
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.3
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    • pp.223-235
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    • 1993
  • Background: Superoxide anion which was produced by macrophage and neutrophil has a defensive role to kill invasive microorganisms and also an injurious role to produce self lung damage. Production of oxygen free radicals including superoxide is a main mechanism of acute lung injury caused by bacterial endotoxin. Endotoxin is known to activate alveolar macrophage to produce increased oxygen free radicals after the stimulation with various biological materials (priming effect). Calcium is a very important intracellular messenger in that cellular process of superoxide production. Method: This experiment was performed to elucidate the effects of endotoxin and calcium on superoxide production by phorbol myristate acetate-stimulated alveolar macrophage and the effect of verapamil on priming effect of endotoxin. Results: 1) Preincubation of macrophages with endotoxin (E. coli 055-B5) primed the cells to respond with increased superoxide production after the stimulation with PMA. Priming with endotoxin ($10^{-1}$ug/ml) produced a maximal enhancement of superoxide production (43%). 2) Verapamil could inhibit the superoxide production by PMA stimulated macrophage regardless of the presence of extracellular calcium. This means that the inhibitory effect of verapamil is caused by a mechanism independent of blocking calcium influx. 3) Verapamil could inhibit the priming effect of endotoxin on alveolar macrophage (from 30% increment to 13% increment) and could inhibit the superoxide production by PMA-stimulated macrophage preincubated with endotoxin. Conclusion: We concluded that verapamil could inhibit the superoxide production by PMA-stimulated rat alveolar macrophage and also inhibit the priming effect of endotoxin on alveolar macrophage. These inhibitory effects of verapamil could be one of the mechanisms of verapamil effects on endotoxin induced lung injury.

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Changes of Components in Salt-fermented Northern Sand Lance, Ammodytes personatus Sauce during Fermentation (숙성기간에 따른 까나리액젓의 성분변화)

  • CHO Young Je;IM Yeong Sun;LEE Keun Woo;KIM Geon Bae;CHOI Yeung Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.6
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    • pp.693-698
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    • 1999
  • To investigate changes of components in salt-fermented northern sand lance, Ammodytes personatus sauce during fermentation, various chemical properties were examined at 1$\~$3 months intervals during 18 months fermentation. The moisture content decreased slightly, but the content of VBN and crude protein, total nitrogen, amino nitrogen, degree of hydrolysis, and absorbance at 453 nm increased gradually during fermentation. On the other hand, ash content, pH, and salinity showed almost no change. The contents of total nitrogen, amino nitrogen, and degree of hydrolysis increased sharply until 6$\~$8 months fermentation and showed the gentle increment after that, The Hx and uric acid were the most abundant in ATP related compounds, ranging from $83.1\%$ to $92.9\%$, After 18 month of fermentation, sauce was rich in free amino acids, such as glutamic acid, alanine, Iysine, leucine, isoleucine, valine, aspartic acid in that order.

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Some Factors Affecting Bone Mineral Status of Postmenopausal Women (폐경 후 여성의 골격상태에 영향을 미치는 요인분석)

  • 오세인;이행신;이미숙;김초일;권인순;박상철
    • Korean Journal of Community Nutrition
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    • v.7 no.1
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    • pp.121-129
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    • 2002
  • Osteoporosis, the typical metabolic bone disease of the elderly, is characterized by a reduction in bone mineral density (BMD) and increased fracture risk. Genetic and environmental factors are known to play a key role in bone metabolism, and diet is also considered to be one of the important factors. The purpose of the present study was to investigate the relationship among the factors affecting BMD, including stature, body weight, age, time period since onset of menopause, and biochemical markers of bone turnover in postmenopausal women. Seventy-eight postmenopausal women who visited health promotion center for health examinations volunteered to participate in this study and they were divided into two groups according to the time period since onset of menopause : women with a time period since onset of menopause of less than 5 years (Group 1) and women with a time period since onset of menopause of 5 years or more (Group 2). The demographic characteristics and dietary intake were surveyed using a questionnaire. BMDs of the lumbar spine and femoral neck of subjects were measured by dual energy X-ray absorptiometry. Serum levels of 25-hydroxy-vitamin D and parathyroid hormone (PTH), known to be indicators of bone related hormone status, were anlyzed. Serum samples were measured for calcium, phosphorus, alkaline phosphatase, and osteocalcin as bone formation indicators, and urine was analysed for deoxypyridinoline, creatinine, calcium, and sodium as bone resorption indicators. The results are as follow : The mean BMDs of the lumbar spin and femoral neck were $1.02 \pm 0.02 g/cm^2 and 0.81 \pm 0.02 g/cm^2 respectively, and the BMD level of Group 2 was significantly lower than tat of Group 1 (p<0.01, p<0.05, respectively). The mean daily intake of energy was 1838 $\pm$ 55 kcal. When nutrient intake was compared with the recommended dietary allowances (RDA) of the subjects, only calcium, vitamin A and riboflavin intake showed means lower than the RDA. The nutrient intake did not show any significant differences between Group 1 and 2 Serum and urine levels of biochemical markers of bone turnover did not show any significant differences between Group 1 and 2, and all were within the normal range. However, the PTH and deoxypyridinoline levels showed a tendency to be higher, and the osteocalcin level to be lower in Group 2 than in Group 1. Although age and years after menopause (YAM) showed negative correlations with lumbar spine bone mineral density (LBMD) (r= -0.38, p<0.001, and r= -0.26, p< 0.05, respectively), no correlation was found with femoral neck bone mineral density (NBMD). While height, body weight and body mass index (BMI) showed a positive correlation with LBMD (r= 0.32, p<0.001, r= 0.38, p<0.001, r= 0.22, p= 0.05, respectively), only body weight and BMI showed a positive correlation with NBMD (r= 0.30, p<0.01, and r= 0.27, p<0.05, respectivley). There was no significant corealtion between BMDs and the nutrient intake of subjects, except in the case of carbohydrates (r= 0.22, p<0.05). Also, serum and urine levels of bone turnover markers showed no significant correlation with nutrient intake. On the other hand, serum osteocalcin had a positive correlation with vitamin C intake (r= 0.22, p= 0.05), and urine deoxypyridinolin showed a negative correlation with niacin intake (r= -0.22, p= 0.05). Urinary na was negatively correlated with protein intake(r= -0.23, p= 0.05). The results suggested that it is difficult to prevent the decrease in bone mass among postmenopausal women eating the usual Korean diet. However, the BMDs of the lumbar spine and femoral neck were positively related to body weight ad BMI in postmenopausal women. Therefore, this study confirmed that one of the most effective ways to minimize bone loss in postmenopausal women would be to maintain an adequate body weight with balanced nutrient intake and activity in the pre-and postmenopausal periods.

The Effects of Addition of Apple Pomace to Rice Straw Silage on Feed Intake and Digestibility of Korean Native Goats (사과박 첨가 볏짚 사일리지가 한국 재래산양의 사료 섭취량과 소화율에 미치는 영향)

  • 조익환;황보순;전하준;안종호;이주삼;한태호
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.2
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    • pp.107-114
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    • 2002
  • Rice straw silage added with apple pomace in different ratios were mixed with commercial concentrates and fed to Korean native goats in comparison to the corn silage mixed with the same content of commercial concentrates in whole diet as that of apple pomace added rice straw silage (rice straw : apple pomace = 100:0, 80:20, 60:40, 40:60). Dry matter intake, digestibilities of nutrients and nitrogen retention were investigated for the possibilities of application of agricultural by-products for the diets of Korean native goats. Crude protein contents of rice straw silage added with apple pomace were 6.3~7.3% and the contents of ADF, NDF and crude ash were highest in 100% rice straw mixed ratio (A) as 39.4, 61.6 and 8.9% respectively. Those were lower in corn silage (E) as 30.3, 53.4 and 4.9% respectively, however NSC content of corn silage was highest among the experimental treatments as 31.4%. Daily dry matter intakes per head and also per metabolic basal weight (DM g/kg of $BW^{0.75}$ were significantly (p<0.05) higher in D of the highest mixed ratio of apple pomace (605.3, 69.5g) than those of corn silage (E: 394.0, 46.8g). Daily live weight changes were significantly (p<0.05) higher in 40% (C: 16.7g) and 60% (D: 22.9g) apple pomace mixed ratios than 0% (A: 0.17g) and 20% (B: 4.3g) apple pomace mixed ratios. Digestibilities of dry matter and organic matter were higher in D and E than in A and B and those of ADF and NDF were higher in D as 50.2 and 57.4% respectively than the digestibilities in A, B and E. Nitrogen retention (g, %) was highest in D of the highest mixed apple pomace ratio (1.4g, 20.4%) however lowest in A (-0.3g, -7.75%).

High Forage Yielding and Good Silage Quality of a New Barley (Hordeum vulgare L.) Cultivar 'Dachung' (조사료 수량이 높고 사일리지 품질이 우수한 청보리 신품종 '다청')

  • Park, Jong-Ho;Cheong, Young-Keun;Kim, Kyong-Ho;Park, Tae-Il;Kim, Yang-Kil;Park, Hyoung-Ho;Park, Jong-Chul;Gang, Cheon-Sik;Song, Tae-Hwa;Han, Ouk-Kyu;Yun, Geon-Sig;Hong, Ki-Heung;Bae, Jeong-Suk;Song, Jae-Ki;Oh, Young-Jin
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.4
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    • pp.301-307
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    • 2017
  • A new barley(Hordeum vulgare L.) cultivar 'Dachung' having high forage yielding and good silage quality was developed at National Institute of Crop Science, RDA in 2015. This cultivar was derived from a cross of the 'Sunwoo' and 'Keunalbori1ho' in 2002. And it's promising line showed both high yield and lodging resistance through the preliminary and advanced yield trials(PYT, AYT) at Iksan from 2010 to 2012. It was designated as the 'Iksan479'. 'Iksan479' was conducted to regional yield trials(RYT) in six locations around Korea for three years from 2013 to 2015. And it was released as the name of 'Dachung'. It has erect plant type, growth habit of IV and green leaf. In the paddy field its heading date was April 24 and maturing date was May 26, same day with 'Youngyang'. Plant height of 'Dachung' was 99cm. Dachung's spikes per $m^2$ was 625. It has high rate of leaf blades, resistance to BaYMV(Barley Yellow Mosiac Virus) and better winter hardiness than that of 'Youngyang'. The average dry matter of 'Dachung' was about $11.9ton\;ha^{-1}$ in paddy field. And average feed quality of 'Dachung' was 9.0% of crude protein content, 31.3% of ADF (Acid Detergent Fiber), 54.4% of NDF (Neutral Detergent Fiber), 64.0% of TDN (Total Digestible Nutrients). 'Dachung' had grade I of silage quality. This cultivar would be suitable for the area above the daily minimum temperature of $-8^{\circ}C$ in January in Korean peninsula.

The Changes of Serum Angiotensin Converting Enzyme Activity in Lung Cancer Patients (폐암 환자의 혈청 Angiotensin Converting Enzyme 활성도의 변화)

  • Jeong, Ki-Ho;Choi, Hyung-Seok;Yoo, Chul-Gyu;Lee, Kye-Young;Kim, Young-Whan;Han, Sung-Koo;Shim, Young-Soo;Kim, Keun-Youl;Han, Yong-Chol
    • Tuberculosis and Respiratory Diseases
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    • v.39 no.4
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    • pp.310-317
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    • 1992
  • Background: Angiotensin converting enzyme is a glycoprotein peptidyldipeptide hydrolase which cleaves the c-terminal dipeptides of several oligopeptides. It is a menbrane-bound protein mainly synthesized by the endothelial cells. Since the lung has the largest capillary bed of any organ in the body, it is here that ACE acts on circulating substrates like angiotensin I and bradykinin. It is well known that ACE correlates with disease activity in sarcoidosis and also there are reports that changes in serum ACE activity are found in many acute and chronic lung diseases. So we planned this study to see if serum ACE activity can act as a prognostic factor in lung cancer. Methods: Forty-one newly diagnosed lung cancer patients were included in the study group. There were 19 patients with squamous cell lung cancer, 13 with adenocarcinoma, and 9 with small cell carcinoma. Patients were excluded from the study if they had high blood pressure, heart disease, liver disease, renal disease, or other lung disease. Serum ACE activity was analyzed according to cell type, staging, mode of treatment, and clinical response to treatment. Results: 1) There was no difference in serum ACE activity between lung cancer patients and the control group. Also no difference in serum ACE activity was found according to cancer cell type or staging. 2) In patients who underwent curative resection of lung cancer, serum ACE activity was decreased significantly after the operation. 3) In patients who were diagnosed as non-small cell lung cancer and were treated with 4 cycles of anti-cancer chemotherapy without clinical improvement, changes in serum ACE activity were not seen after the treatment. 4) In patients diagnosed as small cell lung cancer treated with 4 cycles of anti-cancer chemotherapy with clinical improvement, changes in serum ACE activity were also not observed. Conclusion: Serum ACE activity was decreased after lung resection but had no relation to cell type, staging, or clinical response to treatment in lung cancer patients. Therefore, serum ACE activity is not suitable in predicting clinical outcome of lung cancer patients.

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HER-2/neu Protein Expression in Canine Mammary Adenocarcinoma (HER-2/neu 단백질이 개 유방암에서의 발현분석)

  • Yang, Hai-Jie;Do, Sun-Hee;Yuan, Dong-Wei;Hong, Il-Hwa;Ki, Mi-Ran;Park, Jin-Kyu;Goo, Moon-Jung;Lee, Hye-Rim;Hong, Kyung-Sook;Hwang, Ok-Kyung;Han, Jung-Youn;Park, Ho-Yong;Yoo, Sung-Eun;Jeong, Kyu-Shik
    • Journal of Life Science
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    • v.18 no.1
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    • pp.16-22
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    • 2008
  • In this study to evaluate the involvement of EGFR, HER-2/neu and ALCAM (CD166) oncogene products in canine mammary neoplastic lesions, sections of archived paraffin-embedded samples of 49 mammary tumors were analyzed immunohistochemically using antibodies against human EGFR and HER-2/neu and ALCAM. These 49 tumors were divided into 2 groups: 22 benign (19 adenoma, 3 benign mixed tumors) and 27 malignant tumors (2 simple adenocarcinomas, 5 complex adenocarcinomas, 3 solid carcinoma, 5 sclerosing carcinoma, 8 malignant mixed tumors and 4 malignant myoepithelioma). As a result of immunostaining, 31.8% (7/22) of the benign tumors and 29.6% (8/27) of the malignant tumors expressed the HER-2/neu oncogene product, EGFR expression was detected in 27.3% (6/22) of benign tumors and in 22.2% (6/27) of the malignant tumors. ALCAM expression was detected in 40.9% (9/22) of benign tumors and in 7.4% (2/27) of the malignant tumors. These results suggest that some of the biological and morphological characteristics of the tumor are associated with canine mammary gland tumors, as also reported for human breast cancer, the possibility of using anti-HER-2/neu antibodies in the treatment of canine mammary tumors.

Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates (돈피젤라틴 효소분해물의 항산화 활성 및 신경세포보호효과)

  • Kim, Dong Wook;Park, Kimoon;Ha, Goeun;Jung, Ju Ri;Chang, Ounki;Ham, Jun-Sang;Jeong, Seok-Geun;Park, Beom-Young;Song, Jin;Jang, Aera
    • Food Science of Animal Resources
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    • v.33 no.2
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    • pp.258-267
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    • 2013
  • This study was conducted to determine the antioxidative and neuroprotective effect of pig skin extracts (PS) and pig skin gelatin hydrolysates (LPS) using a human neuroblastoma cell line (SH-SY5Y). The extraction yield of PS was 3 fold higher than that of LPS. The protein content of PS was about 10 fold higher than that of LPS (p<0.05). Also LPS increased antioxidative activity dose dependently, and the activity was significantly higher than PS at all concentration (p<0.05). DPPH radical scavenging activity of LPS at 50 mg/mL was 92.97%, which was similar to $1{\mu}M$ vitamin C as a positive control. ABTS radical scavenging activity of LPS (20 mg/mL) was 89.83% and oxygen radical absorbance capacity of LPS at 1 mg/mL was $141.39{\mu}M$ Trolox Equvalent/g. No significant change of human neuroblastoma cells was determined by MTT test. Cell death by oxidative stress induced by $H_2O_2$ and amyloid beta 1-42 ($A{\beta}_{1-42}$) was protected by LPS rather than PS. Acetylcholine esterase was significantly inhibited, by up to 33.62% by LPS at 10 mg/mL. Therefore, these results suggest that pig skin gelatin hydrolysates below 3 kDa have potential to be used as anti-oxidative and neuroprotective functional additives in the food industry, while further animal test should be determined in the future.

Comparative Study on Eating Habits, Dietary Intake Patterns, and Nutrient Intakes Between Elementary School Children With and Without Atopic Dermatitis (일부 초등학생의 아토피피부염 유무에 따른 식습관, 식품섭취양상 및 영양소섭취상태 비교 연구)

  • Park, Nam-Suk;Jeon, Eu-Sun;Kim, Young-Nam;Cho, Kyung-Dong;Baek, Ok-Hee;Lee, Bog-Hieu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.11
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    • pp.1543-1550
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    • 2009
  • The aim of this study was to examine and compare the eating habits, dietary intake patterns and nutrient intake status of children with and without atopic dermatitis by questionnaire during July, 2008. A total 388 subjects of 5th and 6th grade elementary school children (AG: atopic group, n=65, NG: non-atopic group, n=323) in Seoul and Ulsan areas participated in this study. The questionnaire included general characteristics, dietary habits, and atopy-related food frequency. One-day 24 hour recall was collected to estimate nutrient intakes of the subjects. The data were analyzed by Chi-square test for food frequency analysis and by t-test for nutrient intakes. Atopy-related foods included milk, buckwheat, beef, pork, chicken, crab, egg, mackerel, peach, and tomato. From the findings, AG had a more irregular eating habit than NG (p<0.05). In case of food frequency, AG tended to consume more atopy-related foods than NG (p>0.05). The nutrient intakes of AG were significantly lower than those of NG (p<0.05), but only intake of animal iron in AG was higher than NG (p<0.05). NG consumed more protein than AG (p<0.05). Although milk was a noted hypersensitive allergic food, frequency and the amount of milk intake were not significantly different between two groups. In conclusion, atopic children had eaten more atopy-related foods and less nutrient intakes. Therefore, it is necessary to educate on good nutrition and guide atopic children and their parents.