• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.233-236
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• 2006

Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

• Korean journal of applied entomology
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• v.23 no.1 s.58
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• pp.37-41
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• 1984

The $100\%$ of insect pests on the imported logs and Sitophilus oryzae(L.) will be killed both when the imported logs are fumigated on shipboard with 25g methyl $bromide/m^3$ for 24hours at $10\~20^{\circ}C$ and when they are fumigated on shipboard with 37.5g methyl $bromide/m^3$ caculated by DT products for 16 hours at $10\~20^{\circ}C$ using 2 fans of 1800rpm over and ID 30cm. Without using fans, it takes $4\~6$ hours to make gas concentration even in hold. But using 2 fans per hold, the period reduces to $1\~3$ hours.

• Korean Journal of Medicinal Crop Science
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• v.20 no.2
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• pp.136-139
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• 2012

This study is part of research to develop the technology for managing major medicinal crops after harvest. We studied the optimal germination conditions of $Cynanchum$ $wilfordii$ (Maxim.) Hemsl. The mean germination time (MGT) of $C.$ $wilfordii$ seeds was higher after soaking for 4 days after storage at $5^{\circ}C$ for 8 weeks, than with germination at $25^{\circ}C$ (3 days). However, the germinative energy (GE) decreased as the number of days soaking increased. The greatest germination rate ($83.3{\pm}8.8%$) was at $25^{\circ}C$ with no soaking of seeds stored at $-20^{\circ}C$ for 8 weeks. Based on these results, we characterized the germination conditions of a major medicinal crop.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.432-436
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• 2014

The soil-borne ascomycete fungus Cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin A and radicicol. The slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. In this study, we developed a selective medium for C. destructans using radicicol produced by this fungus. Supplementing 50 mg/L of radicicol to medium inhibited the mycelia growth of other fungi including Botrytis cinerea, Rhizoctonia solani and Alternaria panax, but did not affect the growth of C. destructans. In addition, conidia germination of other fungal species except for C. destructans was inhibited in submerged culture supplemented with radicicol. This medium provides a very efficient tool for isolating C. destructans and also can be used as an enrichment medium for this fungus.

• Korean Journal of Microbiology
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• v.17 no.2
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• pp.58-64
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• 1979

Studies on some properties of cellulase isolated from Piricularia oryzae. Crude cellulases were prepared from dried rice plant powder (Tong-il, Pal-dal) culture of P. oryzae(N-2, C-8, T-2). The best yield of enzyme was obtained from the medium using Tong-il rice plant powder for P. oryzae cav. N-2 and 2%-sucrose concentration in preculture media. Two units of the enzyme were incubated at $60^{\circ}C$ for 1 hour with 1.0ml, 0.6% Na-CMC. The optimum temperature for the enzyme activity was at $60^{\circ}C$ and the optimum pH was at pH4.0. When Na-CMC was used as substrate the $K_m$ values of crude enzyme were calculated to be $1.05{\times}10^{-4}\;mM\;and\;V_{max}$ was 2.8 mmole/hour. A 10-fold partial purification was achieved by $(NH_4)_2SO_4$ precipitation followed by column chromatography on DEAE Sephadex A-25.

• Resources Recycling
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• v.5 no.3
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• pp.31-36
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• 1996

The metallic copper was recovered from sludge of the copper electro-plating plant by pyrometallurgical process. The reducing agent was Pyrolysized from waste tires and the flux was a mixture $Na_2CO_3$, $NaB_4O_7$, and glass. The green sludge contained 87.5% moisture and 12.5% solid with 56.5% Cu and 1.59% Fe. The sludge dried at $100^{\circ}C$ was analyized to be $Cu_4SO_4(OH)_6{\cdot}2H_2O$ and CuO by XRD analysis. The former was 84% and the latter 16%, However, the calcined sludge at $500^{\circ}C$ was 49% $Cu_2O(SO_4)$ and 51% CuO. The sludge could by smelted at $1100^{\circ}C$ for two hours with 6 to 8 moles carbon with respect to copper to produce metallic copper (>90%) with recovery of 9% above.

• Korean Journal of Plant Resources
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• v.4 no.1
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• pp.5-12
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• 1991

A combination of acerocarininc-Wright C-banding technique was utilized to identify each chromosomes in durum wheat ,Triticum durum var. Hordeiforme (2n=4x=28 AABB), This technique elucidated qualitativr and quantitative traits of the indi-vidual chromosomes In coinplement. Most comspicuous bands were observed at thecentromere of B-genome chronmosomes. Each chromosomes of A-genome had some-what weak centromeric, proximal and terminal bands. Chromosomes 2A and 4A hasa small subterminal bands. 6A is smallest and metacentric chromosome and , has two faint interstitial band. Chromosomes 1B and 6B showed satellite and constriction lage band. Short arm of 3B has three heavily interstitial bands. Both arms of chromosome 4B has a lagc centromeric band and a very lage proximal band. 5B had heavilycentromeric band and the long arm showed prominent two interstitial bands. Chromo-somes 25 and 7B has a small terminal band of both arms.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.128.1-128
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• 2003

A leaf blight disease was found on Fatsia japonica plants growing in the first author's apartment garden in May, 2003. Major symptoms were leaf blight and petiole rot. A species of Phytophthora was isolated from the lesions. The isolate readily produced sporangia and sex organs on V8 juice agar plates. Sporangia were papillate, ovoid to subspherical and caducous with a pedicel. Sporangia were 33.6-38.4 ${\times}$ 33.6$\mu\textrm{m}$ with 1/b ratio approximately 1.14, papillae 4-5$\mu\textrm{m}$ high, pedicels also 4-5$\mu\textrm{m}$ long. Oogonia were spherical, 28.8$\mu\textrm{m}$ in diameter. Antheridia were globose, 14.4$\mu\textrm{m}$ in diameter and mating with oogonia paragynously. Mycelia grew best at 30$^{\circ}C$ and did not grow at 35$^{\circ}C$ or above, and at 5$^{\circ}C$. The morphological characteristics conformed to P. cactorum (Leb. And Cohn) Schroeter.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.127.3-128
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• 2003

A leaf blight disease was found on peony plants growing in the first author's apartment garden in May, 2003. A species of Phytophthora was isolated from the lesions. The isolate readily produced sporangia and sex organs on V8 juice agar plates. Sporangia were papillate, ovoid to subspherical and caduceus with a pedicel. Sporangia were 33.6-38.4 ${\times}$ 33.6$\mu\textrm{m}$ with 1/b ratio approximately 1.14, papillae 4-5$\mu\textrm{m}$ high, pedicels also 4-5$\mu\textrm{m}$ long. Oogonia were spherical, 28.8$\mu\textrm{m}$ in diameter. Antheridia were globose, 14.4$\mu\textrm{m}$ in diameter and mating with oogonia paragynously. Mycelia grew best at 30$^{\circ}C$ and did not grow at 35$^{\circ}C$ or above, and at 5$^{\circ}C$. The morphological characteristics conformed to P. cactorum.

• Journal of Radiation Industry
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• v.2 no.3
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• pp.97-104
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which leads a variety of secondary metabolites to participate in differentiation and protection of plant against environmental stresses. In this study, we isolated a full-length cDNA of the C4H gene from a black raspberry (Rubus occidentalis L.), using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of the RocC4H gene contained a 1,515 bp open reading frame (ORF) encoding a 504 amino acid protein with a calculated molecular weight of about 57.9 kDa and an isoelectric point (pI) value of 9.1. The genomic DNA analysis revealed that RocC4H gene had three exons and two introns. By multiple sequence alignment, RocC4H protein was highly homologous with other plant C4Hs, and the cytochrome P450-featured motifs, such as the heme-binding domain, the T-containing binding pocket motif (AAIETT), the ERR triad, and the tetrapeptide (PPGP) hinge motif, were highly conserved. Southern blot analysis revealed that RocC4H is a single copy gene in R. occidentalis.