• Proceedings of the Korean Geotechical Society Conference
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• 2010.09a
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• pp.349-356
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• 2010

In this study, a new design method of pile bent structure considering plastic hinge was proposed on the basis of the beam-column model. Based on the analysis results, it is found that the positioning of plastic hinge on the pile bent structure was influenced by nonlinear behavior of material and p-$\Delta$ effect. Moreover, concrete cracking began to occur at the joint section between the pile and column in case of pile bent structure with different cross-sections. The plastic hinge can be developed on the pile bent structure when large displacement was occurred, and pile bent structures can be maintained well only if it is developed on the column part. Therefore, in this study, the optimized cross-section ratio between column and pile was analyzed to induce the plastic hinge at the joint section between the pile and column. Based on this, the optimized diameter ratio of pile and column can be obtained below the inflection point of the bi-linear curve depending on the relations between column-pile diameter ratio($D_c/D_p$) and normalized lateral cracking load ratio($F/F_{Dc=Dp}$). And through this study, it is founded that in-depth limit($L_{As}$=0.4%) normalized by the pile length($L_P$) are proportionally decreased as the pile length($L_P/D_P$) increases up to $L_P/D_P$=17.5, and beyond that in-depth limit converges to a constant value. Finally, it is found that the proposed limit depth by taking into account the minimum concrete-steel ratio would be more economical design of the pile bent structure.

• Journal of the Korean Chemical Society
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• v.39 no.4
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• pp.257-265
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• 1995

Free amino acids were isolated from milk and their absolute amounts were determined by reversed phase high performance liquid chromatography after derivatization with dansyl chloride. The determination of D- and L-amino acids was based on achiral separation on a C18 column. It was found that milk contained totally 41.00 mg DL-amino acids in 100 mL milk. The level of D-amino acids to L-amino acids was determined by a column-switching system combining an achiral reversed phase separation and chiral chelate additive. The chiral separation was carried out with addition of the chiral Cu(N-benzyl-L-proline)2 chelate to the mobile phase in reversed phase liquid chromatography. It was found that the determination of 16 different amino acids is feasible in the milk sample with a C18 column separation and 12 D-amino acids out of the 16 amino acids can be determined via the column-switching system with chiral separation. 2.05% of D-glutamic acid and 2.93% of D-alanine were found in milk.

• Steel and Composite Structures
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• v.28 no.4
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• pp.449-460
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• 2018

This paper reports the experimental results of three through bolt beam-column connections under pure shear forces using modified push-out tests. The investigated specimens include extended end-plates and six through-bolts connecting square concrete-filled steel tubular column (S-CFST) to steel beams. The main goal of this study is to investigate if and how the mechanical shear connectors, such as steel angles and stud bolts, contribute to the shear transfer mechanisms in the steel-concrete interface of the composite column. The contribution of shear studs and steel angles to improve the shear resistance of steel-concrete interface in through-bolt connections was investigated using tests. The results showed that their contribution is not significant when the beam-column connection is included in the push-out tests. The specimens failed by pure shear of the long bolts, and the ultimate load can be predicted using the shear resistance of the bolts under shear forces. The predicted values of load allowed obtaining a good agreement with the tests results.

• Computers and Concrete
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• v.12 no.4
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• pp.393-411
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• 2013

In the analysis and design of reinforced concrete frames beam-column joints are sometimes assumed as rigid. This simplifying assumption can be unsafe because it is likely to affect the distributions of internal forces and moments, reduce drift and increase the overall load-carrying capacity of the frame. This study is concerned with the relevance of shear deformation of beam-column joints, in particular of exterior ones, on the quasi-static behavior of regular reinforced concrete sway frames. The included parametric studies of a simple sub-frame model reveal that the quasi-static monotonic behavior of unbraced regular reinforced concrete frames is prone to be significantly affected by the deformation of beam-column joints.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.506-514
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• 1987

Peroxidase was purified to a homogeneous state from Castanea Semen by ammonium sulfate precipitation, DEAE-cellulose column chromatography, gel filtration on sephadex G-100 and HPLC, and the purification fold was 65.3. The molecular weight of the enzyme was estimated to be about 35,000 by HPLC. In properties of the enzyme which was purified up to sephadex G-100 column chromatography, the optimum pH and temperature were 5.0 and $50^{\circ}C$, respectively. By heating the enzyme at $80^{\circ}C$ for 1.73 min., the enzyme activity was decreased to 10%. The enzyme was active toward aromatic amines such as o-phenylenediamine and p-phenylendiamine. Kinetic studies indicated a Km of 2.6mM for o-phenylenediamine at an optimal hydrogen-peroxide concentration and a Km of 10mM for hydrogenperoxide at an optimal o-phenylenediamine concentration. Among the reagents tested, L-ascorbic acid and sodium L-ascorbate inhibited significantly the enzyme, while $Ca^{++}$ and $Ba^{++}$ activated the enzyme at the concentration of 1mM and 5mM.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.1
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• pp.138-143
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• 1996

After oral administration of 14C-labelled $N^G$-mono[methyl-14C]-L-arginine into rats, 38.2 % and 14.7 % of the administered radioactivity bad been recovered in the urine and stool during 10 days. In the urine, 59.4 % of the radioactivity was recovered in the first 24-hours and used for the indentification of the formation of methylamine. The strong cation-exchange resin column chromatography showed 6.3 %, 7.4 %, 4.9 %, and 81.5 % of the distributions of radioactivity of the neutral, monomethylamine, basic, and uneluted portions, respectively. The radioactivity of monomethylamine portion reeluted into the column chromatography was 39.5 %. The radioactivities corresponding monomethylamine in the column chromatography, thin-layer chromatography, and thin-layer electrophoresis were 39.5 %, 37.3 %, and 28.8 % of the recovered radioactivity, respectively.

• Journal of the Korea institute for structural maintenance and inspection
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• v.3 no.1
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• pp.147-155
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• 1999

The purpose of this study is to investigate the strength and ductility of reinforced concrete columns subject to axial load experimentally for several variables of reinforcements and propose foundational research date for reinforcement design of column. In the test a total of eleven specimens, which are all $20{\times}20{\times}60cm$ in size and differently reinforced with steel plate, has been used. The main variables of reinforcement considered in the test are the width of steel plate, the thickness of steel plate. Based on the test results, the effect of the main variables on the strength and ductility of reinforced concrete column have been scrutinized. The strength of reinforced concrete columns are that C-2 series on strengthed with 2mm thickness steel plate are smaller than C-4 series on strengthed with 4mm steel plate. Thick steel plate of reinforced expected utilizer than the other on strength increase and specimens to be large width steel plate of each system are the utiltzer on strength increase. Ductility of C-0 specimen is 1.60, C-2 series is 2.38, C-4 series 2.63 Compare efficiency of ductility increase with each specimens, in narrow width condition (2cm, 4cm) C-2 series is more efficiency, in wide width condition (8cm, 10cm) C-4 series is more efficiency.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.398.2-398.2
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• 2002

A column switching HPLC assay was developed to allow the separation and quantitation of cetirizine in human plasma by ultraviolet (UV) detection. Plasma samples were prepared by liquid-liquid extraction. After drying, the residue was reconstituted in 20 mM phosphate buffer (pH 2.8) containing 15% acetonitrile. The samples were initially injected onto a clean-up Capcell Pak MF C18 column. (50 mm $\times$ 4.6 mm I.D.), and the chromatographic region containing the peaks of interest was followed in an analytical C18 microcolumn (250 mm$\times$1.5 mm I. D.) via column switching device. (omitted)

• The Korean Journal of Pesticide Science
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• v.2 no.2
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• pp.59-67
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• 1998

토양중 침투성 살충제 carbofuran과 제초제 petilachlor의 용탈행적을 구명하기 위하여 물리화학적 성질이 상이한 2종의 논토양으로 충전된 토양 column (내경 5cm ${\times}$ 길이 30cm)에 $^{14}C$-표지 화합물을 각각 처리한 후 벼 (Oryza sativa L.)를 생육시키면서 벼를 심지 않은 경우를 대조구로 하여 8주 동안 주당 95.2 ml씩 용탈시켰다. Carbofuran의 경우 토양 column에서 용탈된 $^{14}C$ 방사능의 양은 벼를 재배하지 않은 토양 A와 B에서 각각 총처리 방사능의 74.8와 92.3%였으며, 벼를 재배한 토양에서는 각각 45.1%와 69.7%였다. 반면에 petilachlor의 경우 벼를 재배한 토양 column에서는 각각 총처리 방사능의 2.4%와 5.0%가 용탈되었으며, 벼를 재배한 경우는 각각 3.1%와 8.2%가 용탈되었다. 토양에 처리한 [$^{14}C$]화합물 모두 벼의 생육유무에 관계없이 양이온치환용량, 유기물 및 점토의 함량이 적은 토양 B에서 $^{14}C$ 방사능의 용탈이 증가되었다. 토양 column중 carbofuran의 이동성은 매우 높은 반면에 petilachlor는 매우 낮았으며, 이는 그들의 토양흡착과 수용성에 기인된 것으로 판단된다.

• Applied Biological Chemistry
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• v.24 no.3
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• pp.186-193
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• 1981

Three fractions of carboxymethyl-cellulase (F-I, F-II, and F-III) and ${\beta}-glucosidase$ form Aspergillus niger were partially purified by ammonium sulfate fractionation. Sephadex G-150 and DEAE-Sephadex column chromatography. The optimum conditions such as pH and temperature and thermal inactivation properties of the enzymes were investigated. Arrhenius plots of F-II and F-III appeared as straight lines, whereas that of F-I was biphasic. The Z-values of F-II and F-III were $8^{\circ}C$ and $10^{\circ}C$ respectively, while that of F-I was $4^{\circ}C$ over $60{\sim}70^{\circ}C$ and $383^{\circ}C$ over $70{\sim}98^{\circ}C$. Three fractions and the crude extract of carboxymethyl-cellulase exhibited a similar optimum pH 4.3 and temperature of $60^{\circ}C$, while Z-value of crude extract $(21.5^{\circ}C)$ was much higher than that of the purified enzyme. Maximum activity of both purified and crude extract of ${\beta}-glucosidase$ was shown at pH 4.7 and $60^{\circ}C$, and z-value of the enzyme was $7^{\circ}C$.