• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.177-182
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• 2004

A chill unit has been used to estimate chilling requirement for dormancy release and risk of freezing damage. A system that calculates chill units was developed to obtain site-specific estimates of dormancy release date for grapes and evaluated in Baekgu myun near Kimje City, Chunbuk, Korea from September 2002 to March 2003. The system utilized daily minimum and maximum temperature maps generated from spatial interpolation with temperature correction for topography. Hourly temperature was temporally interpolated from the daily data using a sine-exponential equation (Patron and Logan, 1981). Hourly chill units were determined from sigmoid, reverse sigmoid, and negatively increasing sigmoid functions based on temperature ranges and summed for 24 h. Cumulative daily chill units obtained from measurements did not increase until 20 October 2002, which was used as a start date for accumulation to estimate the dormancy release date. As a result, a map of dormancy release date in the study area was generated, assuming 800 chill units as a threshold for the chilling requirement. The chill unit accumulation system, implemented using Microsoft Visual Basic and C++ (Microsoft, Redmond, WA, USA), runs in the Windows environment with ArcView (ESRl Inc., Redlands, CA, USA).

• Proceedings of the KOSOMBE Conference
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• v.1995 no.05
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• pp.95-100
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• 1995

This study investigated the effects of mechanical factors involved in several corneal refractive surgeries on the surgical outcomes. Then we proposed possible new techniques from the mechanical point of a view utilizing finite element method. The models studied are: circumferential keratetomy, combination of excimer laser photorefractive keratectomy and circumferential keratotomy for myopia treatment, arcuate keratotomy for astigmatism treatment. The cornea was assumed to be nonlinear elastic and almost incompressible material as the most soft tissue in the human body. In the circumferential keratotomy the effect of the incision location was investigated. The angle and location of the incision were varied to predict the surgical outcomes in the arcuate keratotomy. The finite element analysis results showed that the location of incision was a critical factor affecting the surgical outcomes in the circumferential keratotomy. In the combination of the excimer laser photorefractive keratectomy and circumferential keratotomy, it was predicted that the circumferential can increase or decrease the refractive power depending on the incision location or it can be used to adjust the overcorrection of undercorrection. In the arcuate keratotomy for astigmatism, the most diopter changes were predicted when the location and the angle of the incision were 3.0mm from the apex and $90^{\circ}$, respectively. In the arcuate keratotomy, the effects of an incision were studied within the incision area as well as outside the incision area. Also, the arcuate keratotomy with two incisions located on the opposite area of the cornea was also studied. As a conclusion, the finite element method is a useful technique in the area of corneal refractive surgeries to develop new techinques.

• The Korean Journal of Physiology
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• v.19 no.2
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• pp.113-125
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• 1985

Electrophysiological difference of the central and peripheral area of the sinoatrial node in the rabbit was studied by glass microelectrode technique. Effects of $K^+,\;Na^+,\;Cs^+,$ adrenaline and ouabain on the action potential of the two areas were investigated, and transient hyperpolarization ($K^+-induced$ hyperpolarization) which developed following readmission of potassium after having pre-treated with $K^+-free$ Tyrode solution for 10 minutes was analyzed. The results obtained were as follows ; 1) The frequency of the spontaneous action potential recorded in the periphery of the SA node was faster than the central area. Reduction by $Cs^+$ and increase by O mM $K^+$, $10^{-6}M$ adrenaline and $10^{-6}M$ ouabain in the frequency of action potential were noticed more prominently in the peripheral than the central area. On the contrary, the frequency in the central area was more decreased than the Peripheral area by 13 mH $K^+$ and 1 mM $Co^{2+}$. 2) The amplitude of the K+_induced hyperpolarization was very small in the central area but large in the peripheral area. Transient hyperpolarization was abolished by ouabain and low sodium, and decreased by cooling the tissue $(17^{\circ}C)$. 3) By changing the concentration of $Ca^{2+}$ in the perfusate, the amplitude and the rate of transient hyperpolarization were increased in the high $Ca^{2+}$ concentration. It could be concluded that the central area of the SA node is less susceptible to the inhibition of Na-Pump and more susceptible to Ca-blocker and high concentration of $K^+$. The Na-Pump activity of the central area measured by means of transient hyperpolarization is found to be much less active than that of the peripheral area.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.284-292
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• 2011

An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were $60^{\circ}C$ and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, ${\beta}$-xylosidase, ${\alpha}$-L-arabinofuranosidase, ${\beta}$-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.

• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.397-404
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• 2013

Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at $40^{\circ}C$ and pH 7.4. Treatment with $Mg^{2+}$ and $Li^+$ showed a slight decrease in XynA activity; however, treatment with 5 mM $Cu^{2+}$ completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.

• Journal of Microbiology and Biotechnology
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• v.28 no.8
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• pp.1293-1298
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• 2018

Phosphomannomutase (ManB) converts mannose-6-phosphate (M-6-P) to mannose-1-phosphate (M-1-P), which is a key metabolic precursor for the production of GDP-D-mannose used for production of glycoconjugates and post-translational modification of proteins. The aim of this study was to express the manB gene from Escherichia coli in Lactococcus lactis subsp. cremoris NZ9000 and to characterize the encoded enzyme. The manB gene from E. coli K12, of 1,371 bp and encoding 457 amino acids (52 kDa), was cloned and overexpressed in L. lactis NZ9000 using the nisin-controlled expression system. The enzyme was purified by Ni-NTA column chromatography and exhibited a specific activity of 5.34 units/mg, significantly higher than that of other previously reported ManB enzymes. The pH and temperature optima were 8.0 and $50^{\circ}C$, respectively. Interestingly, the ManB used in this study had two substrate specificity for both mannose-1-phosphate and glucose-1-phosphate, and the specific activity for glucose-1-phosphate was 3.76 units/mg showing 70% relative activity to that of mannose-1-phosphate. This is the first study on heterologous expression and characterization of ManB in lactic acid bacteria. The ManB expression system constructed in this study canbe used to synthesize rare sugars or glycoconjugates.

• Journal of Surface Science and Engineering
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• v.43 no.1
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• pp.25-30
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• 2010

Titanium and titanium alloys are widely used for orthopedic and dental implants for their superior mechanical properties, low modulus, excellent corrosion resistance and good biocompatibility. In this study, corrosion behaviors of nanotube formed on the bone plate of Ti-6Al-4V alloy for dental use have been investigated. $TiO_2$ nanotubes were formed on the dental bone plates by anodization in $H_3PO_4$ containing 0.6 wt % NaF solution at $25^{\circ}C$. Electrochemical experiments were performed using a conventional three-electrode configuration with a platinum counter electrode and a saturated calomel reference electrode. Anodization was carried out using a scanning potentiostat (EG&G Co, Model 263A USA), and all experiments were conducted at room temperature. The surface morphology was observed using field emission scanning electron microscopy (FE-SEM) and energy dispersive x-ray spectroscopy(EDS). The corrosion behavior of the dental bone plates was examined using potentiodynamic test(potential range of -1500~2000 mV) in a 0.9% NaCl solution by potentiostat (EG&G Co, PARSTAT 2273. USA). The inner diameter of nanotube was about 150~180 nm with wall thickness of about 20 nm. The interspace of nanotube to nanotube was 50 nm. The passive region of the nanotube formed bone plates showed the broad range compared to non-nanotube formed bone plates. The corrosion surface of sample was covered with corrosion products.

• Proceedings of The Korean Society of Health Promotion Conference
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• 1999.07a
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• pp.9-36
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• 1999

21C기를 맞이하면서 미국의 보건소가 당면한 과제는 시민들을 상해질병에서 보호하고 예방하는 것이다. 금세기 초 남녀 평균수명은 50세에 불과하였으나 후반에 이르러서는 남자 평균 수명 72세, 여자 79세로 늘어나게 되었는데 이것은 공중보건의 기여라고 볼 수 있다. 지난 10년 동안 미국의 보건의료정책은 격변기를 맞았으며 지역사회 병원들은 점차 보건소 활동분야인 예방사업에도 침투하고 있어서 지방보건소 활동과 경쟁하게 되었다. 이로 인하여 지방보건소는 지역사회에서 보다 광범위한 보건증진계획을 세워야 했고 동반자의 참여를 절실해하고 있다. 미국 의학연구소는 "공중보건의 장래" 라는 지침서를 발간하였고 10대 필수보건수칙이 지방보건소를 위하여 정해졌다. 이를 수행하기 위해선 지방보건소가 갖추어야 할 사항은 1) 건강 평가 2) 정책수립 3) 행정력 4) 건강증진 계획 5) 시민건강 보호대책 6) 질적 확인 체재수립 7) 직원교육 훈련 8) 지역사회 자발력 및 참여 고취 등이 있다. 보건교육 사업이란 그 과제와 재능과 개념 등이 다양해 이의 포괄적인 수용이 모색되어야하며, 그래서 이 논문은 건강증진 사업에 대한 "새 눈의 시야"와 같다고 볼 수 있다. "보건교육/보건증진" 이란 공중보건의 이론과 실제를 일체화하는데 있다고 볼 수 있다. 미국은 2010년 보건의료 정책수립에 "건강증진/ 보건교육" 역할을 문서화하였고, "보건교육/ 건강증진" 사업은 지역사회 안에서 체계화하여 "건강한 사회에 건강한 시민" 이라는 사회운동의 청사진으로 이어지고 있다. 이 논문은 콜로라도의 "건강한 사회에 건강한시민" 운동을 한 예로 들고 있으며 "보건교육/건강증진" 사업에 대한 표준 설정을 위하여 노스캐롤라이나주 지방보건소의 "보건교육/건강증진" 표준체제를 예로 들었다. 다음으로, 경제적인 효율면에서 볼 때 "보건교육/건강증진" 사업에는 단기 혹은 장기 투자가치가 있는가 하는 것이다. 새로 태어나는 미국 지방 보건소의 "보건교육/건강증진" 활동은 지역 시민 및 그 단체가 광범위하고도 자연 다발적으로 참여할 때만 성공할 수 있다고 결론 지울 수 있다.

• Journal of the East Asian Society of Dietary Life
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• v.10 no.2
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• pp.136-141
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• 2000

This study was conducted to analyze the properties of the roasted dandelion tea prepared after various pre-treatments such as pre-drying, steaming and air blast drying. The dandelion tea was prepared by roasting the pre-treated dandelion leaves and its total yield, pH, soluble solid content, turbidity, browning and sensory value were evaluated. Total yield and soluble solid content were the highest in case of air blast drying. Turbidity and browning degree were similar in all the teas regardless of their pre-treatment including pre-drying, steaming or air blast drying. The best palatability in the sensory evaluation was obtained at the steaming condition for 60 sec without pre-drying. Longer air blast drying time resulted in the increase in the a and b values. turbidity, browning degree, total yield and soluble solid content in the air blast dried tea.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.4
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• pp.442-451
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• 1999

This study was conducted to investigate the antimicrobial activity of Prunus mume extracts. Prunus mume extracts have growth inhibitory activity to various microorganisms. Gram positive bacteria such as Bacillus cereus, Bacillus subtilis, Micrococcus leteus, Staphylococcus epidrimidis were more easily inhibited than Gram negative bacteria tested Vibrio parahaemolyticus, Salmonella typhimurium, Escherichia coli, Proteus vulgaris and Yeasts by Prunus mume extracts. Among several fractions of methanol extract, EtOAc & BuOH fractions were showed strong antibacterial activities, but those fractions were not showed on fungi.