• Geomechanics and Engineering
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• v.14 no.5
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• pp.407-419
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• 2018

In order to investigate flow characteristics after water inrush from the working face in process of karst tunnel construction, numerical calculation for two class case studies of water inrush is carried out by using the FLUENT software on the background of Qiyueshan tunnel. For each class water inrush from the tunnel face, five cases under different water-inrush velocity are simulated and researched. Three probing lines are selected respectively in the left tunnel, cross passage, right tunnel and in the height direction of the tunnel centerline. The variation characteristics of velocity and pressure on each probing line under the five water-inrush velocities are analyzed. As for the selected four groups probing lines in the tunnels, the change rules of velocity and pressure on each group probing lines under the same water-inrush velocity are discussed. Finally, the water flow characteristics after inrush from the tunnel face are summarized by comparing the case studies. The results indicate that: (1) The velocity and pressure change greatly at the intersection area of the cross passage and the tunnels. (2) The velocity nearby the tunnel side wall is the minimum, while it is the maximum in the middle position. (3) The pressure value of every cross section in the tunnels is basically fixed. (4) As water-inrush velocity increases, the flow velocity and pressure in the tunnels also increase. The former is approximately proportional to their respective water-inrush velocity, while the latter is not. The research results provide a theoretical basis for making scientific and rational escape routes.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1492-1497
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• 2005

The goal of the trial was to evaluate the effects of ractopamine (20 ppm in the ration) given pregnant sows during three different pregnancy stages (T1: 25 to 50 d; T2: 50 to 80 d; T3: 25 to 80 d of gestation, and T4: control-no ractopamine) on fetal muscle development (through counts of the number of fibers of the semitendinosus muscle), on the growth and carcass characteristics of the progeny. Forty eight weaned piglets (12 per treatment) were assessed for number of muscle fibers, while performance and carcass characteristics were evaluated on a separate 48 animals (12 per treatment) grown to 100 kg. Animals produced by sows treated from 25 to 50 d of pregnancy (T1) resulted in non-significant increase of 6.85% in the number of muscle fibers in the semitendinosus muscle when compared to animals from the control group. Performance results were significantly different (p<0.05), and animals produced by sows from the T1 group gained more weight during the growth I stage (25 to 50 kg) and during the total period (6 to 100 kg) (991 vs. 903 grams, and 844 vs. 772 grams, respectively) when compared to controls. For carcass characteristics, results showed that animals produced by the T1 group of sows were heavier at slaughter (p<0.05) when compared to the controls (T4) (100.17 vs. 93.09 kg). There was a positive correlation between number of muscle fibers and bodyweights, carcass weights, Longissimus dorsi muscle depth and dressing out (0.80, 0.86, 0.67, and 0.50, respectively). Sows treated with ractopamine between 25 to 50 d of pregnancy produced piglets that performed better and had superior carcass characteristics than those produced by untreated sows. The ractopamine used for pregnancy sows (25 to 50 d) can be indicated as a device to increase the progeny performance.

• Journal of Powder Materials
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• v.18 no.6
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• pp.552-561
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• 2011

This study attempted to manufacture a Cu-15 at.%Ga coating layer via the cold spray process and investigated the effect of heat treatment environment on the properties of cold sprayed coating material. Three kinds of heat treatment environments, $5%H_2$+argon, pure argon, and vacuum were used in this study. Annealing treatments were conducted at $200{\sim}800^{\circ}C$/1 hr. With the cold sprayed coating layer, pure ${\alpha}$-Cu and small amounts of $Ga_2O_3$ were detected in the XRD, EDS, EPMA analyses. Porosity significantly decreased and hardness also decreased with increasing annealing temperature. The inhomogeneous dendritic microstructure of cold sprayed coating material changed to the homogeneous and dense one (microstructural evolution) with annealing heat treatment. Oxides near the interface of particles could be reduced by heat treatment especially in vacuum and argon environments. Vacuum environment during heat treatment was suggested to be most effective one to improve the densification and purification properties of cold sprayed Cu-15 at.%Ga coating material.

• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.94-100
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• 2006

To isolate a bacterium that produces plant growth promoting hormone, a total of 29 bacteria were obtained from the soil in Gyeongsan, Korea. Among these, 14 strains were selected by their positive reaction on Salkowski to produce auxin. All of these were then tested for their property to produce siderophore using CAS (chrome azurol S) blue agar, and one was chosen for its ability to produce both, auxin and siderophore. This strain, denoted, AHl8, showed 1.5 times higher adventitious root induction rates than controls, using mung-beans. The strain also showed efficient biocontrol properties towards Fusarium-wilt of tomatoes in artificial pot assays. The strain was identified as Bacillus subtilis by 16s rDNA comparison and Biolog analyses. Growth and media conditions for Bacillus subtilis AH1 8 to highly produce siderophore were also investigated.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.988-998
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• 2015

The filamentous fungus Aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. To facilitate higher yields of proteins of interest, we constructed the pAsOP vector to express heterologous proteins in A. oryzae. pAsOP carries a selectable marker, pyrG, derived from Aspergillus nidulans, and a strong promoter and a terminator of the amyB gene derived from A. oryzae. pAsOP transformed A. oryzae efficiently via the PEG-CaCl₂-mediated transformation method. As proof of concept, green fluorescent protein (GFP) was successfully expressed in A. oryzae transformed by pAsOP-GFP. Additionally, we identified a novel fungal α-amylase (PcAmy) gene from Penicillium sp. and cloned the gene into the vector. After transformation by pAsOPPcAmy, the α-amylase PcAmy from Penicillium sp. was successfully expressed in a heterologous host system for the first time. The α-amylase activity in the A. oryzae transformant was increased by 62.3% compared with the untransformed A. oryzae control. The PcAmy protein produced in the system had an optimum pH of 5.0 and optimum temperature of 30^oC. As a cold-adapted enzyme, PcAmy shows potential value in industrial applications because of its high catalytic activity at low temperature. Furthermore, the expression vector reported in this study provides promising utility for further scientific research and biotechnological applications.

• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.219-225
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• 2009

A bacterial strain, JE-34, producing a high concentration of red pigment was isolated from a sediment in East China Sea. It was identified as Zooshikella sp. JE-34 based on the 16S rRNA gene sequence analysis. The red pigment was purified by solvent extraction and HPLC was identified as prodigiosin-like compound. Nutritional and cultural conditions were optimized for the production of prodigiosin-like pigment in the flask level. Optimal culture conditions were at initial medium pH $6.0{\sim}7.0$, $30^{\circ}C$ and 4 days incubation. For carbon and, nitrogen sources were soluble starch and malt extract.

• Journal of Microbiology and Biotechnology
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• v.28 no.4
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• pp.534-541
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• 2018

Kimchi (a traditional Korean fermented vegetable) was prepared with a starter, Lactobacillus zymae GU240 producing ${\gamma}$-aminobutyric acid (GABA), and one precursor of GABA (glutamic acid, glutamic acid monosodium salt (MSG), or kelp extract). L. zymae GU240, an isolate from kimchi, can grow at 7% NaCl and low temperature. Five different kimchi samples were fermented for 20 weeks at $-1^{\circ}C$. Kimchi with starter alone could not produce GABA. The GABA content was highest in kimchi with co-inoculation of the starter and MSG (1% (w/w)). Kimchi co-inoculated with the starter and kelp extract powder (3% (w/w)) had the second highest GABA content. Addition of glutamic acid powder (1% (w/w)) caused a reduction in the pH level of kimchi and growth inhibition of lactic acid bacteria and yeasts. Kimchi samples with MSG or kelp extract showed improvement of sensory evaluation scores. The results demonstrate the possibility to produce kimchi with improved functionality and taste by using L. zymae GU240 as a starter along with a suitable precursor such as MSG or kelp extract.

• Journal of the Korean Society of Safety
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• v.35 no.4
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• pp.23-31
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• 2020

In the construction sector, there were 99 fatalities from falls associated with steel tube and system scaffolds for three years from 2016 to 2018 and out of which 27.3 percent (27 workers) were found to have occurred during installing and dismantling procedure. To erect scaffolding which is installed for the work at height, the work platforms are generally installed first and the safety guardrails are installed and vice versa to dismantle. As a result, workers are exposed to the risk of falling because they erect and dismantle scaffolding without guardrails. To minimize the risk of falling, it is necessary to study a new construction method that can erect or dismantle scaffolding while guardrails are installed (called advanced guardrail system). This study analyzed advanced guardrail system in terms of safety regulation, workability and economic efficiency by investigating the domestic situation on the use of scaffolding, reviewing domestic and foreign standards for guardrails and conducting economic feasibility study. The results of this study is expected to greatly contribute to preventing falls in scaffolding installation and dismantlement procedure. As a follow-up study, empirical research is required including physical test of scaffolding with advanced guardrail system and effectiveness analysis after trial applications.

• Microbiology and Biotechnology Letters
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• v.17 no.2
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• pp.88-93
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• 1989

To improve a new yeast strain capable of converting xylan to ethanol directly, we tried protoplast fusion between xylose fermenting yeast (Candida sp. X-6-41) and xylan assimilating yeast (Crypto-coccus sp. XB-33), finally selected the most promising two fusants (XFU-1 and XFU-2). As the optimum conditions for protoplast formation, the yeast cells were cultured to exponential phase in YPD and YPX containing 0.6M KCI, respectively, and then treated with zymolyase (0.25mg/$m\ell$), cellulase(4mg/$m\ell$) and 100mM 2-mercaptoethanol at pH 8 and 3$0^{\circ}C$. The protoplasts of parental auxotrophs were fused in the presence of 20mM CaCl$_2$and 40% polyethylene glycol(M.W.4000). The physiological and morphological characteristics of the fusants, such as assimilation of carbon sources, cell size, growth rate, xylanase activity and xylan fermentation ability were investigated. Xylanase activity of fusants that cultured in chemically minimal medium was higher than that of fusants that cultured in completed medium, because xylanase producing activity of xylose fermenting yeast(X-6-41) was inhibited by isoleucine.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1875-1879
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• 2007

Strain $CH7^T$, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the South Coast of Korea, was subjected to a polyphasic taxonomic study. $CH7^T$ grew between 5 and $37^{\circ}C$, pH 5.3-10.5, and tolerated up to 13% NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $CH7^T$ was associated with the genus Arthrobacter and phylogenetically closely related to the type strains Arthrobacter tumbae (99.4%) and Arthrobacter parietis (99.1%). However, DNA-DNA hybridization experiments revealed 2.1% and 12% between strain $CH7^T$ and Arthrobacter tumbae and Arthrobacter parietis, respectively. Thus, the phenotypic and phylogenetic differences suggested that $CH7^T$ should be placed in the genus Arthrobacter as a novel species, for which the name Arthrobacter subterraneus sp. novo is proposed. In addition, the type strain for the new species is $CH7^T$ ($=KCTC\;9997^T=DSM\;17585^T$).