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Effects of SDN® as a Microbial Culture on Milk Production, Milk Composition and Somatic Cell Counts of Lactating Cows (미생물 배양액 SDN®의 급여가 착유우의 유생산, 유성분 및 체세포수에 미치는 영향)

  • Kim, Youg-Kook;Lee, S.R.;Back, W.H.;Lee, I.D.;Bae, H.C.;Back, S.H.
    • Journal of Animal Science and Technology
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    • v.49 no.3
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    • pp.359-368
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    • 2007
  • Holstein cows(n=12) were assigned to one of three diets containing control diet and diets containing a microbial culture, 50ml(T1) and 100ml(T2) SDN(R)(amicrobial culture), per day. The basal diet containing concentrate mixture, corn silage and timothy hay were fed midlactating cows for 12 weeks. Milk production tended to be higher for cows fed T2 diet (20.8kg/day) than fed T1(19.7kg/d) and control diet (19.2kg/day).  There was a tendency of an increase in 4% FCM for cows fed T2 diet(19.6kg/day) than T1(18.8kg/d) and control diet(18.4kg/day). Milk components were not found to be different between cows fed control diet and SDN(R) diets. There was a tendency an increase in milk protein for cows fed control diet(3.43%) compared with microbial diets, T1 and T2(3.08% and 3.20%). However, milk protein production was not significantly different between control diet(0.65kg/d) and T1(0.61kg/d) or T2(0.67kg/d). Somatic cell counts for cows fed T1(72,000) and T2(60,000/ml) were lower than cows fed control diet (108,000/ml) (P<0.05). In conclusion, the cows that were fed diets containing SDN(R) as a microbial culture resulted a tendency of an increase in milk production and a reduction of somatic cell counts which indicates improved milk quality and hygiene.

Fermentative Properties and Immunomodulating Activity of Low-sodium Kimchi Supplemented with Acanthopanax senticosus and Glycyrrhizae uralensis Extracts (가시오가피와 감초 추출물을 첨가한 저 나트륨 김치의 발효특성과 면역 활성능)

  • Yu, Kwang-Won;Suh, Hyung-Joo;Hwang, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.25 no.4
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    • pp.878-887
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    • 2012
  • This study investigated the fermentative characteristics and immunomodulating activity in Kimchi added with various salts (salt replacement and herb-salt with Acanthopanax senticosus and Glycyrrhizae uralensis) for the reduction of Na concentration in Kimchi. Kimchi using a salt replacement and herb-salt showed a higher level of acidity (0.8~0.84%) than that of the control (0.7%) at 7-day fermentation. Kimchi using a salt replacement and herb-salt showed a lower level of salinity (1.72~1.98%) than that of control (2.3~2.57%) during fermentation. The growth of Lactobacillus spp. and Leuconostoc spp. recorded the highest level ($2.3{\times}10^8$ and $2.8{\times}10^6cfu/g$, respectively) in control at 6 day-fermentation. However, those levels in Kimchi prepared with salt replacement and herb-salt were $3.5{\sim}5.4{\times}10^8$ and $6.1{\times}10^6cfu/g$, respectively. It is assumed that the high level of acidity of Kimchi prepared with salt replacement and herb-salt was caused by the increase in the growth of Lactobacillus spp. and Leuconostoc spp.. When the macrophage stimulating activity of salt replacement kimchi (Salt-R kimchi) supplemented with hot-water extract from Acanthopanax sentisus (AS) or Glycyrrhiza uralensis (GU) was investigated on aging period, Salt-RA kimchi with AS 5% at 6 days (2.78-fold of saline control at $100{\mu}g/m{\ell}$) and Salt-RG kimchi with GU 5% at 9 days (2.02-fold) significantly increased compared to the Salt-RA kimchi without AS or GU. In addition, Salt-RAG kimchi with AS 3% and GU 3% improved the bitter taste of Salt-RA and potently stimulated the macrophage at 6 days (1.28-fold of Salt-R kimchi) even though its activity was lower than Salt-RA (5%, 1.39-fold).

Genesis and Mineralogical Characteristics of Acid Sulfate Soil in Gimhae Plain -II. Genesis and Distribution of the Soil Clay Minerals (김해평야(金海平野)에 분포(分布)한 특이산성토(特異酸性土)의 생성(生成)과 광물학적(鑛物學的) 특성(特性) -II. 점토광물(粘土鑛物)의 분포(分布) 및 생성(生成))

  • Jung, Pil-Kyun;Yoo, Sun-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.3
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    • pp.168-178
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    • 1994
  • Acid sulfate soils occur extensively in Gimhae area where they have been formed from the brackish alluvial sediments along the sea coast and river estuary. The strong acid environment enhances silicate weathering and thus affects the soil clay minerals. The minerals were identified through chemical, X-ray diffraction and thermal methods. The ratio of $SiO_2$ and $Al_2O_3$ in the clay fractions ranged from 3.14 to 3.77, indicating that the distribution of the clay minerals were 1 : 1 and 2 : 1 minerals. Cation exchange capacity in the clay fractions was low due to high contents of 1 : 1 minerals and hydroxy interlayered vermiculite(HIV). The B and C horizon rich in jarosite have large amounts of yellow streaks which reflect high content of $Fe_2O_3$ and $K_2O$. Vermiculite and illite were quantified from thermogravimetry(TG), kaolin minerals from both TG and differential thermal analysis(DTA), and HIV from X-ray diffraction analysis. The dominant clay minerals were kaolin minerals, vermiculite, illite and HIV. HIV considered to be formed, especially, in acid soil environments. The minor minerals were quarts, feldspar, jarosite, pyrite, hematite and goethite. Kaolin minerals were the most abundant clay minerals throughout the acid sulfate soil. Kaolin minerals, however, increased towards the top of horizons throughout the soils and HIV decreased towards the top of horizons in the soil of Gimhae series and Haecheog series. Alteration of HIV to kaolin minerals during weathering of low pH condition in deep soil horizons may explain the high quantities of kaolin minerals and the relatively low quantities of HIV in the soil at top horizons.

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Bush Growth and Fruit Quality of 'Duke' Blueberry Influenced by Nutritional Composition in Unheated Plastic House (블루베리 '듀크' 품종의 무가온 하우스 재배에서 질소비율 조절에 따른 수체생육 및 과실품질 변화)

  • Cheon, Mi Geon;Kim, Yeong Bong;Hong, Kwang Pyo;Kumar, H.M. Prathibhani C.;Kim, Jin Gook
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.319-325
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    • 2018
  • The aim of the present study was to determine the influence of different fertilizer combinations on the growth, yield, and fruit quality of 'Duke' blueberry cultivar and the water quality of growth medium. The experiment was carried out with three year old 'Duke' blueberry bushes which were cultivated in containers ($60{\times}80{\times}40cm$) filled with 130 L peat moss and 40 L pearlite (v/v). Sawdust was used as the mulch in growth containers. Three different fertilizer combinations (FC) i.e., FC-1 consisted with standard solution, FC-2 consisted with nitrogen reduced by 10% from FC-1, and FC-3 consisted with nitrogen reduced by 20% from FC-1 were tested while, the ground water used as the control. The effects of different fertilizer combinations on shoot diameter, shoot length, number of shoots, leaf length, SPAD value (the relative content of chlorophyll), berry weight, soluble solids content, titratable acidity, and yield per bush in 'Duke' blueberry were examined. Also, the effects of different fertilizer combinations on pH, EC, $NH_4$ and $NO_3$ in 'Duke' blueberry growth medium were monitored. The highest pH and lowest EC, $NH_4$ and $NO_3$ in growth medium was recorded with control treatment during the experiment period. The maximum shoot diameter (3.7 mm) and shoot length (35.7 cm) was recorded for the FC-1. Highest number of shoots (47%) were recorded from 'Duke' blueberry bushes supplemented with FC-1 compared to other treatments. The fertilizer combinations supplemented with nitrogen showed significant influence on leaf length and SPAD value compared to control 'Duke' blueberry bushes. However, the fruit quality attributes, i.e., berry weight, soluble solids content, and titratable acidity were not significant different among fertilizer treatments. The significantly highest yields per bush were recorded for FC-1, FC-2, and FC-3, as 2.2, 2.9, and 2.7 kg, respectively compared to control (0.2 kg). Although, the FC-1 was supplemented with highest nitrogen content it resulted low yield per bush while having high number of shoots and vigorous growth.

Quality Change of Pork Patty Containing Lotus (Nelumbo nucifera) Leaf and Root Powder during Refrigerated Storage (냉장 중 연근 및 연잎분말을 첨가한 돈육 패티의 품질변화)

  • Park, Kyung-Sook;Park, Hyun-Suk;Choi, Young-Joon;Moon, Yoon-Hee;Lee, Kyung-Soo;Kim, Min-Ju;Jung, In-Chul
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1732-1739
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    • 2011
  • This study was carried out to investigate the effect of lotus root and leaf powder on the quality characteristics of pork patty stored at $3^{\circ}C$ for 9 days. The pork patties were of four types: nothing added(control, T0), 0.5% lotus root powder added (T1), 0.25% lotus root and 0.25% lotus leaf powder added (T2), and 0.5% lotus leaf powder added (T3). The $a^*$ value tended to decrease with longer storage period (p<0.05). The $L^*$ and $a^*$ value of T3 had the lowest value among the samples, the $b^*$ value T2 and T3 were higher than those of T0 and T1 (p<0.05). Water holding capacity decreased with longer storage period (p<0.05), the water holding capacity, cooking loss, increase rate of thickness and decrease rate of diameter were not significantly different among the samples. Hardness and chewiness increased and springiness decreased with longer storage period (p<0.05). The pH creased with longer storage period (p<0.05), but the VBN content not changed during storage. The TBARS values increased with longer storage period (p<0.05), and those of T0, T1, T2 and T3 were 4.57, 1.85, 0.43 and 0.41 mg MA/kg, respectively, after 9 days of the storage. The result suggest that the addition of lotus root and leaf powder at the same time, or addition of lotus leaf powder can be applied to pork patty to its functionality.

Comparison of Antioxidant Activity According to Silkworm Cultivars (품종에 따른 국내산 누에의 항산화 활성 비교)

  • Park, Jong Woo;Lee, Chang Hoon;Jeong, Chan Young;Kang, Sang Kuk;Ju, Wan-Taek;Kim, Seong-Wan;Kim, Nam-Suk;Kweon, Hae Yong;Kim, Kee Young
    • Journal of Life Science
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    • v.31 no.11
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    • pp.1010-1018
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    • 2021
  • Although varieties of silkworms, which have recently attracted attention as a health functional food, are being produced, studies on the differences in the functionality of different silkworm varieties are insufficient. Therefore, in this study, the antioxidant activities of different silkworm breeds bred in domestic farms were analyzed, and the potential for their cultivation as specialized varieties with excellent antioxidant function was investigated. To compare antioxidant activity, four varieties of silkworms, white Bakokjam, Golden silk, Yeonokjam, and Hanseongjam, were bred; water and ethanol extracts of these silkworms were prepared on the 3rd and 5th days of the 5th instar larval stage. The highest extraction yield was seen for the water extract from the Golden silk variety on the 3rd day of the 5th instar; the highest total phenolic compound and flavonoid contents were observed for the water extract (86.11±4.04 ㎍/mg GE) and 70% (v/v) ethanol extract (46.70±2.81 ㎍/mg QE). Bakokjam and Yeonokjam exhibited DPPH radical scavenging activity of up to 78% and showed the highest nitrite scavenging activity (85%) at pH 1.2. The maximum SOD-like activity of Yeonokjam was about 47%. Furthermore, 48 ㎍/ml of the Yeonokjam extract showed a reducing power of 0.7 abs, which was the best among the four varieties. Considering these results, the Yeonokjam (on the 3rd day of the 5th instar) had antioxidant activity and represents a silkworm cultivar that would be suitable for cultivation as a health food.

Quality Changes in Various Heat-treated Market Milks during Storage (열처리를 달리한 시유의 저장중 품질 변화)

  • Kwon, Soon-Ha;Ahn, Joung-Jwa;Kwak, Hae-Soo
    • Journal of Dairy Science and Biotechnology
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    • v.16 no.2
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    • pp.90-97
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    • 1998
  • This study was carried out to investigate changes of microbiological and sensory properties in various heat-treated market milks (LTLT, HTST, and UHT milks) stored at 10$^{\circ}C$ during 15d. Titratable acidity (TA) increased with storage, while pH tended to decrease. During the initial 9d, no difference was found in TA, however, after 9d, it was slightly higher in HTST and UHT milks than that in LTLT milk. In LTLT and HTST milks, total viable cells and psychrotrophs were dramatically increased during storage, In addition coliform and pathogenic bacteria were found at 12 and 15d. In UHT milk, total viable cells were found only at 15d. In sensory evaluation, LTLT and HTST milks developed a negligible off-flavor until 9d. At 12d, it became stronger in HTST milk than that in LTLT milk. In UHT milk, off-flavor was detected at 9d and increased rapidly there-after. The degree of off-flavor was little higher in HTST and UHT milks, compared with that of LTLT milk after 9d storage. These observations indicated that LTLT and HTST milks may not be microbiologically acceptable after 5d, while off-flavor was not detectable until 9d, In comparison, UHT milk keeps a good quality in microorganism until 15d, however, it may not be accepted in sensory aspect after 9d storage.

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Effect of Level and Feeding Period of Dietary Monascus Culture on Cholesterol Content of Serum and Meat of Broilers and Growing Ducks (Monascus 배양물의 첨가 급여 수준 및 기간이 육계와 육용오리의 혈청 및 고기의 콜레스테롤 함량에 미치는 영향)

  • Kim S. I.;Lee W. J.;Lee K. H.
    • Korean Journal of Poultry Science
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    • v.32 no.3
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    • pp.171-177
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    • 2005
  • Two experiments were carried out to investigate the effects of level and feeding period of dietary Monascus culture on the performance and cholesterol content of serum and meat in broilers and growing ducks.0.0(C), 0.5(T1), 1.0(T2) and $1.5\%(T3)$ of iwonascus culture which contained 0.5% monacolin-k was added to commercial broiler diets, respectively, and ffd during 2~6 weeks of age in Experiment 1. In Experilnent 2, commercial broiler diet added $1.0\%$ of Monascus culture was fed during $6\~6(C),\;4\~6(T1),\;2\~6(T2)\;and\;0\~6(T3)$ weeks of age, respectively. Three replicates of 16 day-old broilers and 10 day-old growing ducks each were randomly assigned to floor pen, respectively. In Experiment 1, as the level of dietary Monascus culture increased, body weight and feed intake of broilers significantly decreased (P<0.05), whereas those of growing ducks tended to increase without significant differences among treatments. Feed/gain ratio both in broilers and growing ducks showed a trend to increase. Cholesterol contents of serum both in broilers and growing ducks significantly decreased(P<0.05), and those of breast and thigh meat tended to decrease as the level of dietary Monascus culture increased. In Experiment 2, as the feeding period of dietary Monascus culture increased, body weight gain(P<0.05) and feed intake of broilers tended to decrease whereas those of growing ducks increased without significant differences anions treatments. Feed/gain ratio also increased without significant differences. Cholesterol contents of serum both in broilers and growing ducks significantly decreased(P<0.05), and those of breast and thigh meat tended to decrease as the feeding period of dietary Monascus culture increased without significant differences.

Effects of Various Diets on Growth and Body Composition of Juvenile Parrot Fish, Oplegnathus fasciatus (먹이 종류가 돌돔 Oplegnathus fasciatus 치어의 성장 및 체성분에 미치는 영향)

  • Moon Lee, HaeYoung;Nam, Myung-Mo
    • Korean Journal of Ichthyology
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    • v.27 no.4
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    • pp.293-299
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    • 2015
  • The feeding experiment was conducted to investigate the effects of one experimental diet (EDP) and five different commercial diets (CEPs) on growth and body composition for juvenile parrot fish, Oplegnathus fasciatus. An EDP was formulated to contain 50% crude protein (CP) from fishmeal, casein, zein and wheat flour and 15% crude lipid (CL) from squid liver oil. Five CEPs for seawater fish were two domestic E commercial diet (DECD) and C commercial diet (DCCD), three imported H commercial diet (IHCD), L commercial diet (ILCD) and O commercial diet (IOCD) containing 53.1~66.6% CP and 10.7~14.6% CL, respectively. Each diet was fed to triplicate groups of juvenile parrot fish initially weighing $1.14{\pm}.01g/fish$ (mean${\pm}$SD) in a flow-through seawater system with a water temperature of $19.0{\sim}25.0^{\circ}C$. Weight gain (WG) and feed efficiency (FE) were significantly greatest in fish fed the DCCD and IOCD; intermediate responses were observed for fish fed the ILCD, while the IECD, IHCD, and the EDP produced the lowest WG and FE values. Survival with no significant difference approached 100% for fish fed the all six diets in this experiment. Whole-body moisture, protein, lipid and ash contents were not affected by the different type of diets. Therefore, type of diets appeared to be important factor in influencing WG and FE of juvenile parrot fish; the best diets for juvenile parrot fish was determined to be the domestic commercial C and the imported commercial O diets containing high protein (61.3, 66.6%) and lipid (14.6, 13.0%) in natural seawater based on highest WG, and FE, respectively. This study indicates that the two commercially formulated diets containing two highest proteins and lipids used in this experiment could be practical diets for juvenile parrot fish; these differences of growth performance between experimental diet and commercial diets may be reason for different dietary protein and lipid levels.

Structural Analysis of the Unusual Sugar-Containing Oligosaccharides Formed by the Selective Cleavage of Weakly Acidic Polysaccharide (약산성 다당의 선택적 분해 과정에서 얻어진 특이당 함유 Oligo당의 구조적 분석)

  • Shin, Kwang-Soon;Lee, Ho
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1105-1112
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    • 1997
  • By sequential degradation using partial acid hydrolysis of a weakly acidic polysaccharide (GL-4IIb2'), two acidic oligosaccharide fragments, PA-2' and PA-1-III were isolated and their structures were characterized. PA-2' consisted of almost equal proportion of a rhamnose (Rha) and an unusual sugar, 3-deoxy-D-manno-2-octurosonic acid (Kdo). When permethylated oligosaccharide-alditol derived from PA-2' was analyzed by GC-MS, the peak gave the fragment ions at m/z 189 $(bA_1,\;6-deoxyhexose)$ and at m/z 308 $(aJ_2,\;alditol\;from\;Kdo)$. The peak also gave the characteristic ion at m/z 162 but it did not give the fragment ion at m/z 177, suggesting that Kdo is substituted at C5 but not at C4. Methylation analysis also indicated that PA-2' was composed mainly of terminal Rhap and 5-substituted Kdo. When the reduced product from PA-2' was analyzed by $^1H-NMR$, it gave a signal at 5.09 ppm due to an anomeric proton of ${\alpha}-L-Rha$. These results indicated that PA-2' mainly contained ${\alpha}-L-Rhap-(1{\rightarrow}5)-Kdo$. On the other hand, PA-1-III mainly comprised Rha and Kdo in addition to small proportions of arabinose (Ara) and 3-deoxy-D-lyxo-2-heptulosaric acid (Dha). MS analysis of permethylated oligosaccharide-alditols from PA-1-III suggested that the major peak 1P was $Rhap-(1{\rightarrow}5)-Kdo$ whereas the minor peaks 2P and 3P possessed $Araf-(1{\rightarrow}5)-Dha$ unit and these peaks were produced as epimers during reduction of carbonyl groups in Dha.

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