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Changes of Leaf Area Index, Physiological Activities and Soil Water in Tricholoma matsutake Producing Pine Forest Ecosystem (송이산 소나무림 생태계에서 엽면적지수와 생리적활동 및 토양수분의 변화)

  • Koo, Chang-Duck;Ka, Kang-Hyun;Park, Won-Chul;Park, Hyun;Ryu, Sung-Ryul;Park, Yong-Woo;Kim, Tae-Heon
    • Journal of Korean Society of Forest Science
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    • v.96 no.4
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    • pp.438-447
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    • 2007
  • The purpose of this study was to understand the ecosystem structure and function and soil water changes in Tricholoma matsutake producing pine stands. The investigated stands were pine forest in Sogrisan National Park in Chungbuk-do of Korea. For the purpose we investigated main vegetation, leaf area index(LAI) as ecosystem structural factors and measured photosynthesis, transpiration, xylem water potential, and soil water changes as ecosystem functional factors. Vertical vegetation structure of the site was composed of Pinus densiflora as a overstory species, Quercus mongolica as midstory, Rhododendron mucronulatum, R. schlippenbachii and Fraxinus sieboldiana as understory ones. In the stands LAI was 3.8 during June to September, 2.6 in October and 2.1 during November to April. Photosyntheses of the trees were 6.0 to $7.0{\mu}mol\;CO_2/m^2/s$ in August, and for P. densiflora about $4.0{\mu}mol\;CO_2/m^2/s$ and for Q. mongolica $2.0{\mu}mol\;CO_2/m^2/s$ in mid October. However, R. mucronulatum stopped fixing $CO_2$ and F. sieboldiana shed off the leaves already in mid October. Transpirations were 2.5 to $3.5mmol\;H_2O/m^2/s$ in late August and about $1.0mmol/H_2O/m^2/s$ in mid October. Plant water potentials were -10 to -22 bars for P. densiflora and -5 to -12 bars for the other woody species. The lowest potentials was in late August and highest in late October. Soil water in the stand was closely related to topography. Soil water contents were 7 to 11% at the ridge, 8 to 15% at the hillside and 11 to 19% at the base. Soil temperatures were 0.2 to $0.4^{\circ}C$ higher in T. matustuake colony than noncolony. Mid September soil temperature decreased to $19^{\circ}C$ at which T. matsutake forms primordia. In T. matsutake colony soil moisture was 0.5 to 2.0% lower due to metabolism for consuming water. We suggest that the complicate relationships between ecosystem structure and function in Tricholoma matsutake producing pine stand need to be further investigated.

The Empirical Exploration of the Conception on Nursing (간호개념에 대한 기초조사)

  • 백혜자
    • Journal of Korean Academy of Nursing
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    • v.11 no.1
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    • pp.65-87
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    • 1981
  • The study is aimed at exploring concept held by clinical nurses of nursing. The data were collected from 225 nurses conviniently selected from the population of nurses working in Kang Won province. Findings include. 1) Nurse's Qualification. The respondents view that specialized knowledge is more important qualification of the nurse. Than warm personality. Specifically, 92.9% of the respondents indicated specialized knowledge as the most important qualification while only 43.1% indicated warm personality. 2) On Nursing Profession. The respondents view that nursing profession as health service oriented rather than independent profession specifically. This suggests that nursing profession is not consistentic present health care delivery system nor support nurses working independently. 3) On Clients of Nursing Care The respondents include patients, family and the community residents in the category of nursing care. Specifically, 92.0% of the respondents view that patient is the client, while only 67.1% of nursing student and 74.7% of herself. This indicates the lack of the nurse's recognition toward their clients. 4) On the Priority of Nursing care. Most of the respondents view the clients physical psychological respects as important component of nursing care but not the spiritual ones. Specially, 96.0% of the respondents indicated the physical respects, 93% psychological ones, while 64.1% indicated the spiritual ones. This means the lack of comprehensive conception on nursing aimension. 5) On Nursing Care. 91.6% of the respondents indicated that nursing care is the activity decreasing pain or helping to recover illness, while only 66.2% indicated earring out the physicians medical orders. 6) On Purpose of Nursing Care. 89.8% of the respondents indicated preventing illness and than 76.6% of them decreasing 1;ai of clients. On the other hand, maintaining health has the lowest selection at the degree of 13.8%. This means the lack of nurses' recognition for maintaining health as the most important point. 7) On Knowledge Needed in Nursing Care. Most of the respondents view that the knowledge faced with the spot of nursing care is needed. Specially, 81.3% of the respondents indicated simple curing method and 75.1%, 73.3%, 71.6% each indicated child nursing, maternal nursing and controlling for the communicable disease. On the other hand, knowledge w hick has been neglected in the specialized courses of nursing education, that is, thinking line among com-w unity members, overcoming style against between stress and personal relation in each home, and administration, management have a low selection at the depree of 48.9%,41.875 and 41.3%. 8) On Nursing Idea. The highest degree of selection is that they know themselves rightly, (The mean score measuring distribution was 4.205/5) In the lowest degree,3.016/5 is that devotion is the essential element of nursing, 2.860/5 the religious problems that human beings can not settle, such as a fatal ones, 2,810/5 the nursing profession is worth trying in one's life. This means that the peculiarly essential ideas on the professional sense of value. 9) On Nursing Services. The mean score measuring distribution for the nursing services showed that the inserting of machine air way is 2.132/5, the technique and knowledge for surviving heart-lung resuscitating is 2.892/s, and the preventing air pollution 3.021/5. Specially, 41.1% of the respondents indicated the lack of the replied ratio. 10) On Nurses' Qualifications. The respondents were selected five items as the most important qualifications. Specially, 17.4% of the respondents indicated specialized knowledge, 15.3% the nurses' health, 10.6% satisfaction for nursing profession, 9.8% the experience need, 9.2% comprehension and cooperation, while warm personality as nursing qualifications have a tendency of being lighted. 11) On the Priority of Nursing Care The respondents were selected three items as the most important component. Most of the respondents view the client's physical, spiritual: economic points as important components of nursing care. They showed each 36.8%, 27.6%, 13.8% while educational ones showed 1.8%. 12) On Purpose of Nursing Care. The respondents were selected four items as the most important purpose. Specially,29.3% of the respondents indicated curing illness for clients, 21.3% preventing illness for client 17.4% decreasing pain, 15.3% surviving. 13) On the Analysis of Important Nursing Care Ranging from 5 point to 25 point, the nurses' qualification are concentrated at the degree of 95.1%. Ranging from 3 point to 25, the priorities of nursing care are concentrated at the degree of 96.4%. Ranging from 4 point to 16, the purpose of nursing care is concentrated at the degree of 84.0%. 14) The Analysis, of General Characteristics and Facts of Nursing Concept. The correlation between the educational high level and nursing care showed significance. (P < 0.0262). The correction between the educational low level and purpose of nursing care showed significance. (P < 0.002) The correlation between nurses' working yeras and the degree of importance for the purpose of nursing care showed significance (P < 0.0155) Specially, the most affirmative answers were showed from two years to four ones. 15) On Nunes' qualification and its Degree of Importance The correlation between nurses' qualification and its degree of importance showed significance. (r = 0.2172, p< 0.001) 0.005) B. General characteristics of the subjects The mean age of the subject was 39 ; with 38.6% with in the age range of 20-29 ; 52.6% were male; 57.9% were Schizophrenia; 35.1% were graduated from high school or high school dropouts; 56.l% were not have any religion; 52.6% were unmarried; 47.4% were first admission; 91.2% were involuntary admission patients. C. Measurement of anxiety variables. 1. Measurement tools of affective anxiety in this study demonstrated high reliability (.854). 2. Measurement tools of somatic anxiety in this study demonstrated high reliability (.920). D. Relationship between the anxiety variables and the general characteristics. 1. Relationship between affective anxiety and general characteristics. 1) The level of female patients were higher than that of the male patient (t = 5.41, p < 0.05). 2) Frequencies of admission were related to affective anxiety, so in the first admission the anxiety level was the highest. (F = 5.50, p < 0.005). 2, Relationship between somatic anxiety and general characteristics. 1) The age range of 30-39 was found to have the highest level of the somatic anxiety. (F = 3.95, p < 0.005). 2) Frequencies of admission were related to the somatic anxiety, so .in first admission the anxiety level was the highest. (F = 9.12, p < 0.005) 0. Analysis of significant anxiety symptoms for nursing intervention. 1. Seven items such as dizziness, mental integration, sweating, restlessness, anxiousness, urinary frequency and insomnia, init. accounted for 96% of the variation within the first 24 hours after admission. 2. Seven items such as fear, paresthesias, restlessness, sweating insomnia, init., tremors and body aches and pains accounted for 84% of the variation on the 10th day after admission.

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Growth and Survival Rate of Abalone, Sulculus aquatilis in a Closed Recirculating Sea Water System (폐쇄식 순환사육수조에서 오분자기, Sulculus aquatilis 치패의 체중별 성장 및 생존율)

  • 강경호;김재우;김용만;김병학
    • The Korean Journal of Malacology
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    • v.16 no.1_2
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    • pp.31-34
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    • 2000
  • A series of rearing experiments have been conducted to determine the growth and survival rate in accordance with body size in closed recirculating sea water system. The abalone, Sulculus aquatilis, spat of 10.24${\pm}$0.85 mm, 0.36${\pm}$0.l g and 24.9${\pm}$1.07 mm 2.07${\pm}$0.57 g in shell length and body weight were used in this study Ranges of water temperature and pH during the rearing period of 90 days were 10.0-24.2$^{\circ}C$ and 7.8-8.2, respectively. Dissolved oxygen during the experiment period was 6.13-7.21 ml/l and inorganic nitrate was 0.68-3.72 $\mu\textrm{m}$ in NH$_4$$\^$+/-N, 0.17-7.79 fm in NO$_2$-N and 0.4-11.52 $\mu\textrm{m}$ in NO $_2$-N, Growth in shell length and body weight of the spat in large group and small group at the end of the experiment were 30.80${\pm}$3.14 mm, 17.98${\pm}$2.61 mm and 3.20${\pm}$0.8 g, 0.36${\pm}$0.1 g, and survival rate of spat in large group and small group at the end of the rearing experiment were 96.0% and 90.0% , respectively.

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Quality Assurance Program of Electron Beams Using Thermoluminescence Dosimetry (열형광선량계를 이용한 전자선 품질보증 프로그램에 관한 연구)

  • Rah Jeong-Eun;Kim Gwe-Ya;Jeong Hee-Kyo;Shin Dong-Oh;Suh Tae-Suk
    • Progress in Medical Physics
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    • v.16 no.2
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    • pp.62-69
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    • 2005
  • The purpose of this study has been performed to investigate the possibility of external audit program using thermoluminescence dosimetry for electron beam in korea. The TLD system consists of LiF powder, type TLD-700 read with a PCL 3 reader. In order to determine a calibration coefficient of the TLD system, the reference dosimeters are irradiated to 2 Gy in a $^{60}CO$ beam at the KFDA The irradiation is performed under reference conditions is water phantom using the IAEA standard holder for TLD of electron beam. The energy correction factor is determined for LiF powder irradiated of dose to water 2 Gy in electron beams of 6, 9, 12, 16 and 20 MeV (Varian CL 2100C). The dose is determined according to the IAEA TRS-398 and by measurement with a PTW Roos type plane-parallel chamber. The TLD for each electron energy are positioned in water at reference depth. In this study, to verify of the accuracy of dose determination by the TLD system are performed through a 'blind' TLD irradiation. The results of blind test are $2.98\%,\;3.39\%\;and\;0.01\%(1\sigma)$ at 9, 16, 20 MeV, respectively. The value generally agrees within the acceptance level of $5\%$ for electron beam. The results of this study prove the possibility of the TLD quality assurance program for electron beams. It has contributed to the improvement of clinical electron dosimetry in radiotherapy centers.

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Development of Decompressed Dryer for Mushroom (버섯의 감압건조기 개발)

  • 김영민;김유호;조영길;최희석;조광환
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2003.07a
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    • pp.267-272
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    • 2003
  • 본 연구에서는 버섯의 건조시간을 줄여 건조 후 버섯의 품질을 향상시키기 위하여 버섯의 감압건조기를 개발하였으며 연구 결과는 다음과 같다. 가. 버섯의 감압건조기의 시작기를 제작하였고, 시작기의 구조는 건조실, 감압블로워, 흡ㆍ배기구 솔레노이드 밸브, 제습장치, 송풍기, 전기히터로 구성되어 있으며, 건조실의 내부 압력이 표준대기압-900hPa, 열풍온도는 상온에서 5$0^{\circ}C$까지 조절할 수 있도록 제작하였다. 나. 시작기를 이용하여 표고버섯을 대상으로 건조특성시험을 실시한 결과, 열풍온도 5$0^{\circ}C$, 건조실 내부압력 900-940hPa에서 건조시간은 11.5h으로 대기압과 비교하여 6.5h 단축되는 것으로 나타났다. 또한, 건조 전ㆍ후의 색차, 수축률, 복원률의 품위가 우수한 것으로 나타났으며 F-test 유의성 검증 결과, 5%의 유의수준에서 갓주름의 색차, 갓직경의 수축률, 복원률에서 유의차가 있는 것으로 나타났다. 다. 열풍온도 5$0^{\circ}C$, 건조실 내부압력 900-940hPa로 표고버섯과 아가리쿠스에 대하여 건조성능시험을 실시한 결과, 표고버섯의 경우 건조시간은 13h으로 열풍식 농산물 건조기와 비교하여 건조시간이 4.5h 단축되는 것으로 나타났으며, 아가리쿠스의 경우 건조시간이 17.5h로 나타났다. 라. 성능시험 결과, 시작기가 열풍식 농산물건조기와 비교하여 건감률, 색택, 수축률, 복원률 등의 품위가 전반적으로 우수한 것으로 나타났다. 마. 시작기의 건조비용을 분석한 결과, 건조시간은 단축되었지만 시설투자비가 높아 건조비용이 표고버섯의 경우 2,461원/kg으로 열풍식 농산물건조기 보다 13% 높은 것으로 나타났지만, 추후 고품질 건조버섯 생산을 위한 기초자료로 활용이 가능할 것으로 판단된다.라갈수록 낮은 온도분포를 나타내고 있는 것은 상부에는 외부공기가 유입되면서 온도가 떨어지는 반면 하부에는 외부공기 유입이 적기 때문으로 사료된다. 또한 열풍실의 길이방향 위치별 온도 분포에서도 같은 현상으로 나타났고, 버너쪽과 송풍기쪽의 온도편차는 나타나지 않아 균일 건조를 기대할 수 있다. 마. 열풍온도를 45$^{\circ}C$로 설정하고 조사거리와 방사체 길이를 각각 119, 1,470mm로 하여 벼의 건조성능시험을 열풍건조기(대비구)와 비교시험 결과 시험구에서 건감률, 건조소요에너지가 각각 0.58%(w.b.), 470kcal/kg - water로 대비구보다 각각 건감율은 23% 높았고, 건조소요에너지는 2%의 절감되었다. 바. 건조기에서 발생되는 소음은 버너쪽 근처에서는 대비구 94.12㏈의 87%에 불과하였으나, 거리가 멀어질수록 차이는 크지 않았다. 이것은 버너에서 멀어질수록 외부적인 요인이 소음에 영향을 미쳤기 때문인 것으로 생각된다. 사. 시작기와 대비구간의 경제성에서 시작기의 구입가격이 20% 비싸기 때문에 시간당 고정비가 높았으나, 건조성능이 우수하여 건조비용이 69,350원/톤으로 대비구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all

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Can the C-14 Urea Breath Test Reflect the Extent and Degree of Ongoing Helicobacter pylori Infection? (C-14 요소호기검사의 정량치가 Helicobacter pylori 감염 정도를 반영할 수 있을까?)

  • Lim, Seok-Tae;Sohn, Myung-Hee;Lee, Seung-Ok;Lee, Soo-Teik;Jeong, Myoung-Ja
    • The Korean Journal of Nuclear Medicine
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    • v.35 no.1
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    • pp.61-68
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    • 2001
  • Purpose: The C-14 urea breath test (C-14 UBT) is the most specific noninvasive method to detect Helicobacter (H) pylori infection. We investigated if the C-14 UBT can reflect the presence and degree of H. pylori detected by gastroduodenoscopic biopsies (GBx). Materials and methods: One hundred fifty patients (M:F=83:67, age $48.6{\pm}11.2$ yrs) underwent C-14 UBT, rapid urease test (CLO test) and GBx on the same day. For the C-14 UBT, a single breath sample was collected at 10 minutes after ingestion of C-14 urea (137 KBq) capsule and counting was done in a liquid scintillation counter for 1 minute, and the results were classified as positive (${\geq}200dpm$), Intermediate ($50{\sim}199dpm$) or negative (<50 dpm). The results of CLO tests were classified as positive or negative according to color change. The results of GBx on giemsa stain were graded 0 (normal) to 4 (diffuse) according to the distribution of H. pylori by the Wyatt method. We compared C-14 UBT results with GBx grade as a gold standard. Results: In the assessment of the presence of H. pylori infection, the C-14 UBT global performance yielded sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of 92.5%, 88.4%, 97.1%, 88.4% and 91.3%, respectively. However, the CLO test had sensitivity, specificity, PPV, NPV and accuracy of 83.2%, 81.4%, 91.8%, 81.4% and 82.7%, respectively. The quantitative values of the C-14 UBT were $45{\pm}27$ dpm in grade 0, $707{\pm}584dpm$ in grade 1, $1558{\pm}584dpm$ in grade 2, $1851{\pm}604dpm$ in grade 3, and $2719{\pm}892dpm$ in grade 4. A significant correlation (r=0.848, p<0.01) was found between C-14 UBT and the grade of distribution of H. pylori infection on GBx with giemsa stain. Conclusion: We conclude that the C-14 UBT is a highly accurate, simple and noninvasive method for the diagnosis of ongoing H. pylori infection and reflects the degree of bacterial distribution.

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Development of Revegetation Methods Using Fresh Woodchip from Construction Works (건설현장 발생재를 활용한 비탈면 녹화에 관한 연구)

  • Nam, Sang-Jun;Kim, Kyung-Hoon;Yeo, Hwan-Joo;Jung, Ji-Jun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.3
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    • pp.86-95
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    • 2004
  • This study was conducted to develop recycle revegetation methods for the restoration of the steep slopes by using fresh wood chip from construction sites. In general, the fresh wood chips can be used as soil media for the restoration works, because they can increase infiltration of rainfall and give enough porous to breathe and elongate for the root growth as well as economic value. The experiment was carried out to compare the effect of fresh wood chips from different mixing with soil, organic material and macromolecular compound which used for slope restoration works conducted by Hyunwoo green(Ltd.). The main results by monitoring for two years are summarized as follows; 1. The soil media made with low percentage of fresh wood chip covered quickly by herb plants. Especially, the soil mixture Type C (wood chip 20%) showed 80 percent ground coverage within two months after seeding. 2. The soil mixture type E (wood chip 40%) and type F (wood chip 50%) which contains more fresh wood chips than soil type C was under 30 percent ground coverage because wood plants are germinated well. If the restoration works aims at making forest, then the soil type E and F would be recommended than using soil type C. 3. Among the woody plants, Ailanthus alfissima, Pinus rigida, Pinus densiflora, and Albizzia julibrissin showed high percentage of germination rates and vigorous growth. In case of shrubs, Lespedeza cyrtobotria and Indigofera pseudo-tintoria scored high percentage of germination rates. 4. In native plants, Chrysanthemum indicum, Artemisia princeps, Lutos corniculatus and Imperata cylindrica showed high percentage of appearance. In case of introduced herbs, Coreopsis lanceolata, Coreopsis tinctoria and Oenothera oborata grew so vigorously. 5. The soil types which including fresh wood chips over 30-40 percentage showed the most diverse plant composition and the most effective germination rates and growth pattern with woody plants. 6. This works to develop recycle revegetation methods using fresh wood chips need more efforts for monitoring the exact effect of fresh wood chips as the soil media.

A Calculation of the Coefficients for Estimating the Regional Radiation in Using the penman Equation (Penman식의 적용에 있어서 지역별 일사량 추정을 위한 계수의 산정)

  • Ko, Heui-Weon;Hwang, Eun;Kim, Shi-Won
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.31 no.4
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    • pp.96-110
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    • 1989
  • To suggest the fundamental data for the estimation of crop evaportranspiration by the ca- lculated coefficients for estimating the radiation suitable to the different regions of korea in application of Penman equcation, the daily data such as sc(skycover), n(actual sunshine hours), N(possible sunshine hours), Rs(horizontal solar radiation) and Ra(extraterrestial solar radiation) for 10 years (from 1977 to 1986) collected from 19 meteorological stations were analysed. The results are summarized as follows : 1. The coefficients a, b and c for estimating the radiation taken by the regression method with the daily and monthly mean data of the skycover and the ratio of Rs to Ra were shown as a=0.619, b= -0.0202, c= -0.0023 and a=0.64, b=0.0377 c=0.0001 in ave- rage respectively. 2. The coefficients a and b for estimating the radiation analysed by the regression and arithmetic method from the daily ratio of sunshine hours and Rs to Ra were shown as a= 0.157, b= 0.529, and a=0.119, b= 0.726 in average, respectively. 3. The coefficients a and b for estimating the radiation calculated by the regression me- thod based on the monthly ratio of sunshine hours and radiation were shown as a=0. 319 and b= 0.557 in average. 4. The values of a and b for estimating the radiation taken from the relationship between the daily ratio of sunshine hours and radiation showed high significance level. 5. The standard deviation and the coefficient of variance between the radiation calculated from the coefficients by the regression and arithmetic method with the daily data and the actual radiation were analysed and compared to the results by the coefficients of the modified Penman method (a=0.18, b=0.55) and by those of the F.A.O inodified Penman method(a=0.25, b=0.5). The standard deviation and the coefficient of varia- nce by the regression method in this study showed the lowest value. 6. From the above results, it is suggested that regression method using the coefficients taken from the relationship between the ratio of sunshine hours and the ratio of radia- tion based on the daily data has the highest accuracy in estimating the radiation. 7. The average reference crop evapotranspiration estimating by the modified Penman me- thod using the coefficients a and b derived by the regression method from the daily meterological data was closer to the actual evapotsranspiration of grass measured in Suwon area than the estimated evapotranspiration by the modified Penman method and the F.A.O modified Penman method.

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Phenolic acids in Panax ginseng inhibit melanin production through bidirectional regulation of melanin synthase transcription via different signaling pathways

  • Jianzeng Liu ;Xiaohao Xu ;Jingyuan Zhou;Guang Sun ;Zhenzhuo Li;Lu Zhai ;Jing Wang ;Rui Ma ;Daqing Zhao;Rui Jiang ;Liwei Sun
    • Journal of Ginseng Research
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    • v.47 no.6
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    • pp.714-725
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    • 2023
  • Background: Our previous investigation indicated that the preparation of Panax ginseng Meyer (P. ginseng) inhibited melanogenesis. It comprised salicylic acid (SA), protocatechuic acid (PA), p-coumaric acid (p-CA), vanillic acid (VA), and caffeic acid (CA). In this investigation, the regulatory effects of P. ginseng phenolic acid monomers on melanin production were assessed. Methods: In vitro and in vivo impact of phenolic acid monomers were assessed. Results: SA, PA, p-CA and VA inhibited tyrosinase (TYR) to reduce melanin production, whereas CA had the opposite effects. SA, PA, p-CA and VA significantly downregulated the melanocortin 1 receptor (MC1R), cycle AMP (cAMP), protein kinase A (PKA), cycle AMP-response element-binding protein (CREB), microphthalmia-associated transcription factor (MITF) pathway, reducing mRNA and protein levels of TYR, tyrosinase-related protein 1 (TYRP1), and TYRP2. Moreover, CA treatment enhanced the cAMP, PKA, and CREB pathways to promote MITF mRNA level and phosphorylation. It also alleviated MITF protein level in α-MSH-stimulated B16F10 cells, comparable to untreated B16F10, increasing the expression of phosphorylation glycogen synthase kinase 3β (p-GSK3β), β-catenin, p-ERK/ERK, and p-p38/p38. Furthermore, the GSK3β inhibitor promoted p-GSK3β and p-MITF expression, as observed in CA-treated cells. Moreover, p38 and ERK inhibitors inhibited CA-stimulated p-p38/p38, p-ERK/ERK, and p-MITF increase, which had negative binding energies with MC1R, as depicted by molecular docking. Conclusion: P. ginseng roots' phenolic acid monomers can safely inhibit melanin production by bidirectionally regulating melanin synthase transcription. Furthermore, they reduced MITF expression via MC1R/cAMP/PKA signaling pathway and enhanced MITF post-translational modification via Wnt/mitogen-activated protein kinase signaling pathway.

Whitening activity of Ficus carica L. fruits extract through inhibition of tyrosinase and MITF expression (무화과(Ficus carica L.) 열매 추출물의 tyrosinase 및 MITF 발현 억제를 통한 미백 활성)

  • Min Ji Kim;Si Eun Park;Geun soo Lee;Jin Hwa Kim;Sunwoo Kwon;Hyung Seo Hwang
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.204-212
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    • 2023
  • Whitening is inhibitory activity of the melanin synthesis of melanocytes. Recently, whitening materials have been developed on natural materials because of its side effects on skin. Figs (Ficus Carica L.) is a fruit belonging to the Moraceae family and whitening activity was reported in focusing on the fig's stem and leaf components, but whitening activity of the figs fruit was not known. Thus, in this study, we tried to observe its anti-melanogenesis as well as antioxidant and anti-inflammation. The radical scavenging activity of figs fruits extract (FFE) was observed as the level of 34.52±1.98%/60.71±1.26% compared to the control in the its maximum concentration in the DPPH/ABTS assay. Cytotoxicity of FFE was observed at 10% concentration by CCK8 assay, so the maximum concentration was set at 5% and applied to all experiments. FFE concentration dependently decreased NO production associated with inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6 and tumor necrosis factor-α gene expression, these strongly suggesting anti-inflammatory activity. In melanin contents assay, FFE significantly down-regulated melanin production in α-MSH-stimulated B16F10 cell as well as tyrosinase inhibition in vitro. In addition, FFE decreased the Microphthalmia-associated transcription factor (MITF) mRNA expression about 94.34% compared to the α-MSH treatment group in RT-PCR. Finally, FFE significantly reduced the MITF, cAMP response element-binding protein and tyrosinase protein expression in the α-MSH stimulated B16F10 cell. Through these results, we found that FFE can not only directly inhibit tyrosinase enzyme activity but also suppress melanogenesis through regulation of MITF gene expression in α-MSH signal transduction.