• 제목/요약/키워드: C.E.P.

검색결과 5,775건 처리시간 0.038초

교란점토의 압축지수 및 팽창지수의 관계에 대해서 (Relationship Between Compression Index and Swelling Index for Remolded Clays)

  • 박종화;고우모또 타츠야
    • 한국농공학회:학술대회논문집
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    • 한국농공학회 2002년도 학술발표회 발표논문집
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    • pp.317-320
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    • 2002
  • The compression index, $C'_c$ and the swelling index, $C'_s$ which are obtained from the e-log p curve of the consolidation test, are used to calculate the consolidation settlement. In this research, $C'_c$ and the two new methods of $C'_s$ i.e. $C'_{s1}$, $C'_{sm}$ for remolded clays were examined with the $({\omega}_0-{\omega}_p)$ and $(e_0-e_p)$ which are satisfied with the condition and the type of soil. Moreover, the relationships of $C'_{s1}$, and $C'_{sm}$ versus $C'_c$ were obtained.

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진해만 해산 지각류의 계절적인 소장 (Seasonal Distribution of Marine Cladocerans in Chinhae Bay, Korea)

  • 유광일;김세화
    • 한국해양학회지
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    • 제22권2호
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    • pp.80-86
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    • 1987
  • 1982년에서 1983년에 걸쳐 진해만에서 채집, 동정된 해산 지각류는 다음의 5 종이였다 : Evadne nordmanni, Evadne tergestina, Podon leuckarti, Podon polyphemoides, Penilia avirostris. 계절적인 출현양상을 보면 충계에는 E. nordmanni(3-5월), P. leuckarti(4-5월),그리고 하계에서 추계에 걸쳐서는 Evadne tergestina(6-12월), P. avirostris(6-11월)가 탁월하였다. 한편 P. Polyphemoides는 2월을 제외하고 년중 출현하였다. 개체수의 분포는 P. avirostris, P. polyphemoides 그리고 E. tergestina의 순서로 많았고 E. nordmanni나 P. leuckarti는 1,000개체/㎥ 미만이었다. 체장조성의 계절적인 변화를 보면 E. tergestina와 P. avirostris는 수온 의 상승에 따라 체장이 큰 폭으로 증가하는 경향을 보이고 있으나, 특히 P. polyphemoides에서만은 수온 15$^{\circ}C$이하에서 체장의 증가를 보였다.

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Development and Characterization of Expression Vectors for Corynebacterium glutamicum

  • Lee, Jinho
    • Journal of Microbiology and Biotechnology
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    • 제24권1호
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    • pp.70-79
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    • 2014
  • In an attempt to develop a variety of expression vector systems for Corynebacterium glutamicum, six types of promoters, including $P_{tac}$, $P_{sod}$, $P_{sod}$ with a conserved Shine-Dalgarno (SD) sequence from C. glutamicum, $P_{ilvC}$, $P_{ilvC}$ with a conserved SD-1 ($P_{ilvC-M1}$), and $P_{ilvC}$ with a conserved SD-2 ($P_{ilvC-M2}$), were cloned into a modified shuttle vector, pCXM48. According to analysis of promoter strength by quantitative reverse transcription PCR, $P_{sod}$ and $P_{sod-M}$ were superior to tac and ilvC promoters in terms of transcription activity in C. glutamicum. All of the promoters have promoter activities in Escherichia coli, and $P_{sod-M}$ displayed the highest level of transcriptional activity. The protein expression in constructed vectors was evaluated by measuring the fluorescence of green fluorescent protein (GFP) and SDS-PAGE. C. glutamicum harboring plasmids showed GFP fluorescence with an order of activity of $P_{ilvC}$ > $P_{ilvC-M1}$ > $P_{sod}$ > $P_{ilvC-M2}$ > $P_{sod-M}$, whereas all plasmids except pCSP30 with $P_{sod}$ displayed fluorescence activities in E. coli. Of them, the strongest level of GFP was observed in E. coli with $P_{sod-M}$, and this seems to be due to the introduction of the conserved SD sequence in the translational initiation region. These results demonstrate that the expression vectors work well in both C. glutamicum and E. coli for the expression of target proteins. In addition, the vector systems harboring various promoters with different strengths, conserved SD sequences, and multiple cloning sites will provide a comfortable method for cloning and gene expression, and consequently contribute to the metabolic engineering of C. glutamicum.

Effects of Dietary Vitamins C and E on Egg Shell Quality of Broiler Breeder Hens Exposed to Heat Stress

  • Chung, M.K.;Choi, J.H.;Chung, Y.K.;Chee, K.M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권4호
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    • pp.545-551
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    • 2005
  • A feeding trial was conducted to determine whether dietary vitamin C (200 mg/kg) and vitamin E (250 mg/kg) prevent any drops in egg shell quality under heat stress in broiler breeder hens. One hundred and sixty molted Ross broiler breeders were housed randomly in an individual cage at 83 weeks of age. Four dietary treatments with forty hens and four replications per treatment were control (no additional vitamins), vitamin C-, or vitamin E-supplemented and combined supplementation of the two vitamins. After a tenday-adaptation period at 25$^{\circ}C$, the ambient temperature was kept at 32$^{\circ}C$ for a three-week-testing period. Egg production dropped dramatically over week but it did not show a significant change among treatments (p<0.05). However, egg quality parameters such as egg weight, specific gravity, shell thickness, SWUSA, puncture force and shell breaking strength from the birds fed the diet with the combined vitamins C and E were significantly improved over those of the control group during the heat stress period (p<0.05). The hens fed the vitamin C diet improved tibia breaking strength (37.16 kg), statistically higher than the birds fed the control and the vitamin E diets (p<0.05). The hens fed the control diet showed higher serum corticosterone levels, a mean of 5.97 ng/ml, than those of the other treatments (p<0.05). The heat stress resulted in elevated heterophils and decreased lymphocytes in serum, increasing the H/L ratios for all the treatments. However, the increases in H/L ratios were alleviated by feeding the diets containing vitamin C alone or together with vitamin E, although there were no significant differences in the ratio between the two groups (p<0.05). In conclusion, vitamins C (200 mg/kg) and/or E (250 mg/kg) supplemented to the diets for broiler breeder hens could prevent drops in egg shell quality and tibia bone strength under highly stressful environmental temperatures.

고추 논 재배시 일라이트부직포 터널을 이용한 막덮기 재배효과 (Effect of Illite Non-Woven Fabric Tunnels on the Growth and Yield of Pepper (Capsicum annuum L.) in Paddy Culture)

  • 장길수;김찬용;권오훈;전수경;황지은;권태영
    • 원예과학기술지
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    • 제33권3호
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    • pp.326-330
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    • 2015
  • 본 시험은 노지 논고추 터널 재배시 피복용 재료에 따른 고추의 생육 및 수량에 미치는 영향을 조사하기 위하여 수행되었다. 터널 피복용 재료는 일라이트 부직포, P.E 비닐을 사용하였다. 기온과 지온은 일라이트 부직포 및 P.E 비닐 터널 처리구에서 노지보다 각각 $7-8^{\circ}C$, $2-3^{\circ}C$ 높았고, 상대 습도도 터널 설치구가 노지보다 30% 더 높게 유지되었다. 고추의 생육과 수량은 일라이트 부직포 및 P.E 비닐 터널 설치구에서 높았고, 10a당 수량은 일라이트 부직포 및 P.E 비닐을 이용한 터널 재배시 노지 재배 대비 각각 30%, 26% 증수되어 고추 논재배시 수량확보를 위한 재배법으로 적용이 가능할 것으로 판단된다.

Effect of Different Raising Techniques on In vivo Performance and Carcass and Meat Traits of Ischia Grey Rabbit

  • Bovera, Fulvia;Di Meo, Carmelo;Nizza, Sandra;Piccolo, Giovanni;Nizza, Antonino
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권9호
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    • pp.1361-1366
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    • 2008
  • One-hundred and twenty Ischia grey rabbits, traditionally raised in pits, were equally divided after weaning (32 days) into three groups: group C, housed in cages (4 rabbits/cage) and fed a commercial concentrate; group GF, housed as C group and fed grasses collected on the island and crushed faba beans supplemented with an appropriate mineral vitamin mix; group P, housed in pits (8 rabbits/pit) and fed as GF group. Feed intake was recorded daily and live weight monthly up to slaughter (92 days). At slaughter, 10 rabbits per group were used to measure carcass and meat traits. The carcasses were weighed and measured according to the standard procedures and meat samples from the Longissimus dorsi and left hind leg were analysed for water holding capacity and chemical composition, respectively. During the entire trial, group C consumed significantly (p<0.01) a higher quantity of feed than the other groups (126.1 vs. 63.4 and 66.5 g/d, resp. for groups C, GF and P) and at slaughter showed a significantly (p<0.01) higher body weight (2,529.7 vs. 1,324.4 and 1,375.4 g, resp. for groups C, GF and P). Significant differences (p<0.01) were found also for dressing out percentage (68.6 vs. 66.6 and 66.9%, resp. for groups C, GF and P) and for meat chemical composition, in particular lipid percentage (4.13 vs. 1.84 and 1.93%, resp., for groups C, GF and P, p<0.01) and moisture (73.7 vs. 76.4 and 76.3%, resp. for groups C, GF and P, p<0.01). The results suggest the opportunity to obtain heavier animals raised in the pits if their diets were integrated with commercial feed.

양송이 버섯의 MAP및 CA저장 효과 (Effect of M.A.P. and C.A. storage on quality of Mushrooms(Agaricus bisporus) during storage)

  • 김준한;김종국
    • 한국식품저장유통학회지
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    • 제2권2호
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    • pp.225-232
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    • 1995
  • In order to study the effect of modified atmosphere packaging(M.A.P.) and controlled atmosphere(C. A.) storage on keeping freshness of mushrooms (Agricus-bisporus). Mushrooms was packaged with polyethylene(P.E.) film (40${\mu}{\textrm}{m}$, 60${\mu}{\textrm}{m}$, 80${\mu}{\textrm}{m}$) and C.A. conditions(CO2 concentration of 2%, O2 concentration of 2%) and storage at 0$\pm$1$^{\circ}C$, RH(relative hummidity) 92$\pm$1%. Gas composition in film was changed rapidly at early of storage, but it kept a constant level after 14 days of storage, and then kept at the level of 6~9% CO2 and 2~5% O2. Weight loss was 5% in non-packed mushrooms after 3 days of storage, but P.E. film, CO2 treated, C.A. conditions were 5% after 21 days of storage. Flesh firmness of the mushrooms was continuously decreased throughout storage period and the lowest of flesh firmness changed was 80${\mu}{\textrm}{m}$-P.E. film packing. Discoloration of the piteous of mushrooms appears to be the most Important factors to determine its marketability, L value of it appears to be of the high values at P.E. film packing and C.A. conditions during period. Large amount of ethanol and acetaldehyde were produced from the 7 days during storage, large contents of mannitol and trehalose were at the 14 days and 7 days during storage.

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Effect of Vitamin C and E on Hepatic Biliary and Microsomal Function in Hepatic Ischemia/reperfusion

  • Kim, Soon-Ae;Seo, Min-Young;Cho, Tai-Soon;Lee, Sun-Mee
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1996년도 춘계학술대회
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    • pp.205-205
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    • 1996
  • 본 실험은 간장허혈 및 재관류시 야기되는 간장 손상에 대해 vitamin C와 E 각각의 효과와 이들의 병용효과를 알아보고자 하였다. 실험군은 흰쥐에 vitamin E(25mg/kg)를 실험전 3일간 투여한 군, vitamin C(100mg/kg)를 실험 5분전 경정맥주사한 군 및 vitamin C와 E의 병용 투여군등의 3군으로 하여 각각에 허혈을 유발시킨 후 (60분) 재관류 1시간, 5시간에 간세포 손상정도(AI.T, AST, liver wet-weight to dry-weight ratio), 지질과산화(MDA), 담즙분비변동(bile flow, bilirubin, cholate output) 및 약물대사효소계의 변동(cytochrome P$_{450}$, aminopyrine-N-demethylase, aniline p-hydroxylase activity) 등을 관찰하였다. 실험결과로는 허혈 및 재관류로 인한 ALT, AST MDA는 재관류 5시간에 최고치를 이루었으며 이는 vitamin C와 vitamin E의 각각 투여로 억제되었고, 특히 vitamin C와 E의 병용투여로 더욱 현저하게 억제되었다. 간세포 부종의 지표인 liver wet-weight to dry-weight ratio도 vitamin C와 E의 병용투어로 유의성있게 억제되었다. 담즙분비량 및 담즙산량은 vitamin C 투여와 vitamin C와 E 병용투여로 허혈 및 재관류로 감소된 양을 증가시켰고, 특히 vitamin C와 E의 병용투여는 담즙분비량에 있어 현저한 상승을 나타내었다. 허혈 및 재관류로 인한 cytochrome P$_{450}$양의 감소와 aminopyrine N-demethylase 활성의 억제는 vitamin C 투여와 vitamin C와 E의 병용투여에 의해 유의성 있게 증가하였다. 이상의 결과로 보아 vitamin C와 vitamin E는 각각 허혈 및 재관류로 인한 간장손상을 완화시켰으며 특히 vitamin C와 E의 병용투여는 상승적으로 적용하여 간세포손상을 더욱 억제시킴을 알 수 있었다.

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한국 담수산 복모류 Chaetonotidae과의 분류 (Freshwater Chaetonotid Gastrotrichs in Korea)

  • 이지민;장천영
    • Animal Systematics, Evolution and Diversity
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    • 제16권1호
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    • pp.87-104
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    • 2000
  • 1994년 5월부터 남한의 다향한 담수역 65개 지점에서 채집한 표본들을 관찰한 결과 모두 10종의 담수산 복모류를 동정하였다.: Polymerurus rhomboides(Stokes), P. nodicaudus(Voigt), Lepidodermella squamata (Dujardin), Chaetonotus(Zonochaeta) succinctus Voigt, C. (Z.) bisacer Greuter, C. (Euchaetonotus) polyspinosus Greuter, C. (E.) heterospinosus Balsamo, C. (E.) heideri Brehm, C. (E.) zelinkai Grunspan, and C.(Hystricochaetonotus) persetosus Zelinka. 저자들은 이들을 재기재하고 도판과 검색표를 작성하였다. 본 연구는 한국 담수산 복모류에 관한 최초의 기록이다.

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윤충란 정량검사를 위한 Stoll 희석충란계산법과 Kato 후층도말변법의 비교 (Comparative Study on the Effectiveness of Modified Kato's Cellophane Thick Smear and Stoll's Dilution Egg Counting Technique for Quantitative Fecal Examination of Helminth Eggs)

  • 홍성종;우호춘
    • Parasites, Hosts and Diseases
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    • 제30권2호
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    • pp.141-146
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    • 1992
  • Kato후층도말변법 (M.C.T.S.)과 Stoll 회석충란계산법(D.E.C.T)으로 회충, 괸충, 간흡 충란 양성자 197명에 대하여 충란 정량검사를 실시하고 그 결과를 비교하여 상관함수식을 도출하였다. M.C.T.S.법에 소요된 검경시간은 표본 1매당 평균 12.6분으로 D.E.C.T.법의 14.6분보다 짧았으며, 층계부하가 낮은 감염자에 있어서도 위음성이 적었다. 각 윤충류 충란에 있어서 M.C.T.S.으로 얻은 결과를 대변 1 g당 충란수(E.P.G.)로 바꾸어 주는 전환함수식은 회충의 경우 E.P.G.=47.86×100.87 logM.C.T.S., 편충은 E.P.G.=41.69×100.83 logM.C.T.S., 간흡충은 E.P.G=63.10×100.55 logM.C.T.S.이었다. 장내 윤충류 감염에 대한 정량적 대변검사법으로 Kato후층도말변법이 Stoll희석충란계산 법보다 더 유용하다고 생각되었다.

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