• Proceedings of the KIEE Conference
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• 1988.07a
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• pp.644-646
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• 1988

This paper has been aimed to develop M68020 Software Development System on a host computer 3B20S for the TDX-2 fully electronic Switching system by transporting M68020 C Cross Compiler. M68020 C Cross Compiler source code which includes assembler, run-time library and optimizer has been analyzed for the installation on the host computer 3B20S system. Moreover, the linkage editor source file has been analyzed and installed on the3B20S to produce the executable file correctly. Through these procedures, the M68020 object codes could be obtained on the 3B20S computer for the multi-using purposes. It has also been confirmed that the M68020 Software Development System on the 3B20S works correctly.

• Fisheries and Aquatic Sciences
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• v.23 no.2
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• pp.3.1-3.8
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• 2020

Background: The IGF system plays important roles in controlling growth, development, reproduction, and aging of organisms. Methods: To estimate maturation of the Pacific oyster Crassostrea gigas, we investigated the expression of insulin-like growth factor (IGF) system components and sex-specific genes. To determine the role of the IGF system in the growth and spawning period of female and male oysters, we examined mRNA expression levels of the C. gigas insulin receptor-related receptor (CIR), IGF binding protein complex acid labile subunit (IGFBP_ALS), and molluscan insulin-related peptide (MIP), as well as those of vitellogenin (Vg) and receptor-type guanylate cyclase (Gyc76C) in gonads of C. gigas collected between April and October, when sex can be determined visually in this species. Results: We found that MIP, IGFBP_ALS, and CIR mRNA expression levels were dependent on sex and month and were greater in males than in females. CIR and Vg mRNA expression levels were very similar among females, whereas IGF system components and Gyc76C were very similarly expressed among males. The highest expression values were observed in May, when oysters are mature; CIR and Vg mRNA expression levels were highest in females, and those of MIP, IGFBP_ALS, CIR, and Gyc76C were highest in males. Interestingly, we observed a 1:1 proportion of females to males during this period. Conclusion: Our results suggest that IGF system components, as well as Vg and Gyc76C, are associated with sexual maturation in C. gigas.

• Transactions of Materials Processing
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• v.20 no.7
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• pp.473-478
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• 2011

In this study, the performance of a thermo siphon type radiator made of copper for LED lighting system was evaluated by using an inverse heat transfer method. Heating experiments and finite element heat transfer analysis were conducted for three different cases. The data obtained from experiments were compared with the analysis results. Based on the data obtained from experiments, the inverse heat transfer method was used in order to evaluate the heat transfer coefficient. First, the heat transfer analysis was conducted for non-vacuum state, without the refrigerant. The evaluated heat transfer coefficient on the radiator surface was 40W/$m^2^{\circ}C$. Second, the heat transfer analysis was conducted for non-vacuum state, with the refrigerant, resulting in the heat transfer coefficient of 95W/$m^2^{\circ}C$. Third, the heat transfer analysis was conducted for vacuum state, with refrigerant. For the third case, the evaluated heat transfer coefficients were 140W/$m^2^{\circ}C$. Third, the heat transfer analysis was conducted for vacuum state, with refrigerant. For the third case, the evaluated heat transfer coefficients were 140W/$m^2^{\circ}C$ for the radiator body, 5W/$m^2^{\circ}C$. Third, the heat transfer analysis was conducted for vacuum state, with refrigerant for the rising position of radiator pipe, 35W/$m^2^{\circ}C$. Third, the heat transfer analysis was conducted for vacuum state, with refrigerant. For the highest position of radiator pipe, and 120W/$m^2^{\circ}C$ for the downturn position of radiator pipe. As a result of inverse heat transfer analysis, it was confirmed that the thermal performance of the current radiator was best in the case of the vacuum state using the refrigerant.

• Solar Energy
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• v.6 no.2
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• pp.3-14
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• 1986

It is desirable to collect the solar thermal energy at relatively high temperature in order to minimize the size of thermal storage system and to enlarge the scope of solar thermal energy utilization. In this study, to develop a solar collector that has both advantages of collecting solar thermal energy at high temperature and fixing conveniently the collector system for long term period, a cylindrical parabolique concentrating solar collector (M.C.P.C.S.C) was designed, which has several rows of parabolique reflectors and thin thickness such as the flat-plate solar collector, maintaining the optical form of concentrating solar collector. The thermal performance of the M.C.P.C.S.C. newly designed in this study was analysed theoretically and experimentally. The results are summarized as follows: 1) prediction equation for outlet temperature, $T_o$, of heat transfer fluid and for the thermal efficiency, ${\eta}$, of the collector were derived as; o $$T_o=[C+B1_n(\frac{I_c(t)}{pv^3})]T_i$$ o $${\eta}=\frac{A}{A_c}\dot{m}[(C-1)+B1_n(E{\cdot}di^6\frac{I_c(t)}{\dot{m}^3})]\frac{T_i}{I_c(t)}$$ 2) When the insolation on the tilted solar collector surface, $I_c$, was $900-950W/m^2$ and the heat transfer fluid was not circulated in tubular absorber, the maximum temperature on the absorber surface was $100-118^{\circ}C$, this result suggested that the heat transfer fluid could be heated up to $98-116^{\circ}C$. The maximum temperature on the absorber surface was decreased with the increase of the collector shape factor, $L_p/L_w$ 3) There was a good agreement between the experimental and theoretical value of solar collector efficiency, ${\eta}$, which was proportional to the collector shape factor, $L_p/L_w$ 4) It is desirable to continue the study on the relationship between the collector shape factor, $L_p/L_w$, and the thermal efficiency of solar collector.

• Journal of Navigation and Port Research
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• v.30 no.1 s.107
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• pp.113-117
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• 2006

The object qf this experiment was the evaluation of the growth rate of frost layer conditioned by inlet air's velocity, temperature and relative humidity on the copper tube in evaporator. In this experiment, the inlet air's velocity were $0.3^m/_s,\;0.6^m/_s,\;0.9^m/_s,$ temperature were $15^{\circ}C,\;20^{\circ}C,\;25^{\circ}C$ and the variation of relative humidity was $70\%~90\%$. And the brine temperature flowing through the copper tubes was kept $-15{\circ}C$ because generally cooling temperature range is constantly $-15^{\circ}C$ in the heat exchanger for air conditioning system It was found that the amount of frost generation increased so that the relative humidity, velocity and temperature of supply air increased.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.563-570
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• 2004

The insulin-like growth factor(IGF) system, consisting of IGFs-I and -II ligands and their receptors and six IGF-binding proteins(IGFBPs), plays an important role in survival, proliferation and differentiation of a variety of cell types. Lithium is a known modulator of survival and proliferation of many cell types in vitro. The present study was undertaken to investigate the relationship between LiCI-induced changes in cell survival and growth and the expression of the IGF system components in C6 rat glioma cell line which, besides IGF-I and its receptor, is known to express IGFBP-3 as its major IGF carrier. When C6 cells were cultured for 24h in the absence or presence of 2mM or 5mM LiCl in a 10% serwn-containing medium, the viability and the number of cells were not affected by added lithium. In 72-h culture, however, C6 cells clearly exhibited a dose-dependent response to added LiCl. The cells cultured for 72h in the presence of 0, 2mM and 5mM LiCl exhibited a typical mitotic, a growth-arrested and an apoptotic appearances, respectively. Moreover, the apoptotic cells were accompanied by reduced expression of IGF-I, IGF-I receptor and IGFBP-3 as examined by semi-quantitative reverse transcription-polymerase chain reaction. Interestingly, blockade of IGFBP-3 mRNA translation by addition of 101${\mu}M$ IGFBP-3 anti-sense oligodeoxyribonucleotide in serum-free, 24-h culture resulted in a decrease in the number of cells as well as relative abundance of the target mRNA. In summary, results suggest that the cytotoxic effect of lithium in C6 cell is likely to be mediated, in part, by suppression by this agent of the expression of the IGF system components. In this regard, IGFBP-3 may play at least a 'permissive' role in normal proliferation of this cell.

• Magazine of the Korean Society of Agricultural Engineers
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• v.41 no.1
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• pp.60-71
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• 1999

This study was performed to improve underirable warm greenhouse environment by fog cooling system in summer season. The resultsof droplet size analysis and cooling effects for fog cooling system are summarized as follows ; 1. At the pump pressure of 70kgf/$\textrm{cm}^2$ , the mean (SMD) drop size was 22.6${\mu}{\textrm}{m}$ and the maximum and minimum drop size was 45.68${\mu}{\textrm}{m}$ and 1.73${\mu}{\textrm}{m}$ , respectively, and almost all of the drop size was less than 40${\mu}{\textrm}{m}$. 2. The temperature of fog cooling greenhouse with 60% shading was dropped more than 2$^{\circ}C$ below the ambient temperature , while the greenhouse temperature without shading was 1$^{\circ}C$ higher than the ambient temperature. 3. It was found that fog spraying intervals were significantly influential on cooling effect. 4. When the greenhouse was ventilated sufficiently by natural vent system, green house temperature could be maintained by 2.5$^{\circ}C$ lower than the ambient temperature, while it was difficult to drop the greenhouse temperature below ambient temeperature without sufficient ventilation. 5. It was found that the temperature of experimental greenhouse could be maintained 3$^{\circ}C$ to 14$^{\circ}C$ lower that of control greenhouse though there were variations depending on experimental and weather conditions.

• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.296-303
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• 1994

An immobilized Trigonopsis variabilis cells having an high activity of D-amino acid oxidase(DAO) was used to convert CPC into GL-7-ACA. The optimal pH of the reaction system was 8.0-8.5, and the optimal temperature was 40$\circ$C. When immobilized cell was used repeatedly in semi-batchwise reaction, the system retained 80% of the initial activity after used of 12 times for over 12 hours. The storage stability of the immobilized cell was maintained for 30 days at 4$\circ$C. The CPC concentration for the maximal reaction rate was about 30 mM and 40 mM for free and immobilized cells, respectively. Substrate inhibition of CPC concentration more than 50 mM was overcomed by 20~25% by immobilization. Pure oxygen supply into reaction system was most efficient in D-amino acid oxidase reaction. Continuous conversion to GL-7-ACA from CPC has been developed with an bioreactor system containing immobilized T variabilis cells. By opera- tion of the reactor for 5 hours, the average conversion yield of >80% and GL-7-ACA production of 40~45 mM per hour could be obtained.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.32-37
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• 1997

The rate constants and activation energies for formation of two chemical markers, M-1 and M-2 at sterilization temperatures were determined in a meatball system. Destruction rates for bacterial spores were also determined. The rate constants for M-1 and M-2 formation at $121^{\circ}C$ were 0.03 and 0.28 Abs/min, respectively. The activation energies for M-1 and M-2 were 27.9 and 24.6 Cal/mol. M-2 was formed faster than M-1 and reached a maximum and decreased. M-1 formation continued up to 30 min at $121^{\circ}C$ and 10 min at $131^{\circ}C$, which makes M-1 a more useful chemical marker for high $F_0$ values. The D-values for spores (B. stearothermophilus ATCC 12980) at 111, 114.4, 117.7 and $121^{\circ}C$ were 7.5, 4.5, 1.9 and 0.58 respectively. At temperatures between 111 and $121^{\circ}C$, there was a liner correlation between destruction of the spores and the M-1 formation. It was difficult to get accurate D-value at $126^{\circ}C\;and\;131^{\circ}C$, because almost all spores were dead before temperature at the center of the meatball reached $126^{\circ}C$. These data suggest that the chemical marker should be used to evaluate overprocessing as well as microbial lethality in aseptic processing.

• Journal of Life Science
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• v.29 no.9
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• pp.949-954
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• 2019

Membrane topology is a key characteristic of membrane proteins. We previously reported the cloning of the chromosome 4 open-reading frame 32 (C4orf32) gene as a potential membrane protein; however, the cellular localization and membrane topology of C4orf32 was as yet unknown. In this study, we found that green fluorescent protein (GFP) fused to the C-terminus of C4orf32 (C4orf32-GFP) was localized to the endoplasmic reticulum (ER). We applied three tools to identify determinants of C4orf32 topology: protease protection, fluorescence protease protection (FPP), and an inducible system using the ternary complex between FK506 binding protein 12 (FKBP), rapamycin, and the rapamycin-binding domain of mTOR (FRB) (the FRB-rapamycin-FKBP system). Using protease protection and FPP assays, we found that the GFP tag in C4orf32-GFP was localized to the cytoplasmic surface of the ER membrane of HeLa cells. Protease protection and FPP assays are useful and complimentary tools for identifying the topology of GFP fusion membrane proteins. The FRB-rapamycin-FKBP system was also used to study the topology of C4orf32. In the absence of rapamycin, a monomeric red fluorescent protein-FKBP fusion (mRFP-FKBP) and C4orf32-GFP-FRB were localized to the cytoplasm and the ER membrane, respectively. However, in the presence of rapamycin, the mRFP-FKBP was shifted from the cytoplasm to the ER and colocalized with the C4orf32-GFP-FRB. These results indicate that the FRB moiety is facing the cytoplasmic surface of ER membrane. Overall, our results clearly suggest that C4orf32 belongs to the family of type I ER resident membrane proteins.