• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권5호
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• pp.2338-2356
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• 2019

Vehicular ad-hoc network (VANET) is the name of technology, which uses 'mobile internet' to facilitate communication between vehicles. The aim is to ensure road safety and achieve secure communication. Therefore, the reliability of this type of networks is a serious concern. The reliability of VANET is dependent upon proper communication between vehicles within a given amount of time. Therefore a new formula is introduced, the terms of the new formula correspond 1 by 1 to a class special ST route (SRORT). The new formula terms are much lesser than the Inclusion-Exclusion principle. An algorithm for the Source-to-Terminal reliability was presented, the algorithm produced Source-to-Terminal reliability or computed a Source-to-Terminal reliability expression by calculating a class of special networks of the given network. Since the architecture of this class of networks which need to be computed was comparatively trivial, the performance of the new algorithm was superior to the Inclusion-Exclusion principle. Also, we introduce a mobility metric called universal speed factor (USF) which is the extension of the existing speed factor, that suppose same speed of all vehicles at every time. The USF describes an exact relation between the relative speed of consecutive vehicles and the headway distance. The connectivity of vehicles in different mobile situations is analyzed using USF i.e., slow mobility connectivity, static connectivity, and high mobility connectivity. It is observed that $p_c$ probability of connectivity is directly proportional to the mean speed ${\mu}_{\nu}$ till specified threshold ${\mu}_{\tau}$, and decreases after ${\mu}_{\tau}$. Finally, the congested network is connected strongly as compared to the sparse network as shown in the simulation results.

• 약학회지
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• 제52권3호
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• pp.225-231
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• 2008

To clarify effects of the structural changes of Fur protein on the resistance to metronidazole (Mtz), the mutational analysis of structure and function of the protein in Helicobacter pylori (Hp) was undertaken. It was identified that some changes in Hp Fur protein resulted in increase of resistance to Mtz, and other changes resulted in decrease of resistance. Increase of Mtz resistance came from the enzyme's decreased ability of reducing prodrug Mtz to the form of bactericidal agent. Some sites that affects Mtz resistance (i) in Fur's N terminal extension, and (ii) in its central region, which links DNA binding and Fe-binding modules were identified. It was also found that the addition of FLAG tag to Fur's C terminus also significantly impairs Fur function.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.363-370
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• 2012

The outer dense fiber 2 (ODF2) protein is an important component of sperm tail outer dense fiber and localizes at the centrosome. It has been reported that the RO072 ES cell derived homozygote knock out of ODF2 results in an embryonic lethal phenotype, and XL169 ES cell derived heterozygote knock out causes severe defects in sperm tail development. The ODF2s splicing variant, Cenexin1, possesses a C-terminal extension, and the phosphorylation of serine 796 residue in an extended C-terminal is responsible for Plk1 binding. Cenexin1 assembles ninein and causes ciliogenesis in early stages of the cell cycle in a Plk1-independent manner. Alternatively, in the late stages of the cell cycle, G2/M phase, Cenexin1 binds to Plk1 and results in proper mitotic progression. In this study, to identify the in vivo function of Plk1 binding to phosphorylated Cenexin1 S796 residue, and to understand the in vivo functional differences between ODF2 and Cenexin1, we generated ODF2/Cenexin1 S796A/Cenexin1 WT expressing transgenic mice in a RO072 ES cell derived $ODF2^{+/-}$ knock out background. We observed a severe defect of sperm tail development by ectopic expression of Cenexin1 S796A mutant and no phenotypic differences between the ectopic expression of ODF2/Cenexin1 WT in $ODF2^{+/-}$ background and in normal wild type mice.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.34-35
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• 2003

Before making of tea, the number of leaf, plant height and node length in young shoot were 5.2, 14.9cm and 1,9cm respectively. These contents caused problems in leaf rolling and uniformity. No significant difference in quality and comoposition of roasted and steamed were observed, external shape and internal quality, however, were good from 1st to 3rd leaf. Chemical nutrition consists of leaf position, Total nitrogen content of terminal leaf was 4.88%, total free amino acid 21.12%, and caffein 3%. Vitamin C content was increased with increasing of leaf age. Making of roasted tea was required long time because camellia leaf was very hard and smoothly. Products had lower water color, perfume and taste. Internal quality of steamed tea was good in water color and taste. The contents of total nitrogen, total free amino acid, catechin, caffeine and vitamin C were 4.24%, 1.01, 17.7%, 2.6% and 75.7mg/ml.(중략)

• 기본간호학회지
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• 제23권2호
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• pp.172-183
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• 2016

Purpose: The purpose of this study was to explore the subjective experience of life-sustaining treatment care among nurses in intensive care units. Method: A phenomenology was used for the study. Data were collected from October to December, 2015 using open-ended questions during in-depth interviews. Participants were nurses working in intensive care units and were contacted through purposive techniques. Eight nurses participated in this study. Results: Four categories emerged from the analysis using Colaizzi's method: (a) difficulties due to life-sustaining treatment care, (b) dilemma of extension or cessation of life-sustaining treatment, (c) repressed feelings and emotional exhaustion, and (d) forming values for life-sustaining treatment from nursing experience. Conclusion: Provision of clearer guidelines on life-sustaining treatment which reflect a family-oriented culture is important for nurses in ICU and will promote nurses involvement in the decision-making process of life-sustaining treatment of patients.

• BMB Reports
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• 제34권6호
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• pp.502-508
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• 2001

A cDNA, encoding the human growth hormone (hGH), was synthesized based on the known 191 amino acid sequence. Its codon usage was optimized for a high level expression in Escherichia coli. Unique restriction sites were incorporated throughout the gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoRI and HindIII sites. The whole fragment was synthesized by an overlapped extension of eight long synthetic oligonucleotides. The four-short duplexes of DNA, which were first formed by annealing and filling-in with a Klenow fragment, were assembled to form a complete hGH gene. The hGH was cloned and expressed successfully using a pET17b plasmid that contained the T7 promoter. Recombinant hGH yielded as much as 20% of the total cellular proteins. However, the majority of the protein was in the form of insoluble inclusion bodies. N-terminal amino acid sequencing also showed that the hGH produced in E. coli contained formyl-methionine. This study provides a useful model for synthesis of the gene of interest and production of recombinant proteins in E. coli.

• 한국미생물·생명공학회지
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• 제41권4호
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• pp.391-397
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• 2013

본 연구에서 584개의 아미노산(68.7 kDa)으로 구성된 cyclomaltodextrinase (LLCD)의 유전자를 Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435)로부터 클로닝하였다. LLCD는 일반적인 CDase 계열 효소들과 약 40% 전후의 아미노산 서열 상동성을 나타내었다. C-말단에 6개의 히스티딘 잔기를 가진 재조합 효소는 dimer의 형태로 대장균에서 발현되고 정제되었다. LLCD는 pH 7.0 및 $37^{\circ}C$에서 최대의 ${\beta}$-CD 가수분해 활성을 나타내었다. 특히, 이 효소는 starch 및 pullulan에 대해 극히 낮은 활성을 보였으나, 반면에 CD에 대한 가수분해 활성은 starch에 비해 약 80배 이상 높았다. 이처럼 높은 CD에 대한 활성을 근거로 LLCD는 CDase 계열 효소로 분류될 수 있으나, starch, pullulan, 그리고 acarbose에 대한 매우 낮은 활성은 다른 유사효소와 비교하여 차별화되는 특징이다.

• 정보처리학회논문지C
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• 제9C권3호
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• pp.343-350
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• 2002

본 논문에서는 영상회의 시스템에서의 사용자 인증 및 미디어 스트림의 암호화를 지원하기 위한 H.235 프로토콜의 구현에 대하여 기술한다. H.235 프로토콜은 ITU-T에서 권고된 H.323 영상회의의 보안 프로토콜로써 불법적인 공격자에 의한 도청 및 조작 방지를 위한 프로토콜이다. 본 논문의 구현에서는 패스워드 기반의 대칭키 암호 인증 방법을 사용하고, Diffie-Hellman 키 분배 알고리즘과 대칭키 암호 알고리즘인 RC2, DES, Triple-DES를 사용하여 미디어 스트림의 암호화를 구현한다. 또한, 추후의 확장성을 고려하여 차세대 표준 암호인 128비트 AES와 한국형 암호인 128비트 SEED를 포함시켜 구현한다. 구현된 인증 방법과 미디어 스트림의 암호화는 네트워크 상에서 개인적인 정보를 노출시키지 않으면서 터미널의 사용자를 확인하는 것이 가능하고, 영상회의의 기밀성을 유지시켜줌을 알 수 있다. 또한, 미디어 스트림의 암호/복호화를 지원해 주면서도 암호화에 따른 지연시간과 메모리가 증가하지 않음으로써 영상회의 시스템의 성능을 저하시키지 않음을 알 수 있다.

• Clinics in Shoulder and Elbow
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• 제8권2호
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• pp.97-104
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• 2005

Purpose: To report the results of surgical treatment of comminuted fractures of distal humerus and to identify factors that affect the results. Materials and Methods: Thirty-two patients who were treated with open reduction and internal fixation for comminuted fracture of distal humerus were enrolled. According to the AO classification, A2.3 was 1 case, A3.2, 2 cases, A3.3, 8 cases, B1.3, 1 case, B2.3, 1 case, C2.2, 5 cases, C2.3, 4 cases, C3.2, 3 cases and C3.3, 7 cases. As fixation technique, 17 cases were fixed by double plates, 4 cases by only K-wires, 4 cases by only screws, 3 cases by K-wires and screws and 4 cases by one plate and screws. The mean age at the time of the operation was 49 years(range, $19{\sim}77$ years). The mean follow-up period was 16 months(range, $8{\sim}51$ months). Results: At the last follow-up, the mean maximum flexion was $116.4^{\circ}\;(range,\;85{\sim}140^{\circ})$ and the mean loss of terminal extension was $11.8^{\circ}\;(range,\;0{\sim}40^{\circ})$. The average Mayo elbow performance score was $91.4^{\circ}\;(range,\;55{\sim}100^{\circ})$. Overall 29 cases(91%) showed good to excellent results. The mean range of motion of extraarticular and intraarticular fracture group was $105.5^{\circ}\;(range,\;65{\sim}140^{\circ})$ and $104^{\circ}\;(range,\;55{\sim}140^{\circ})$, respectively. The average elbow score of both groups was$93^{\circ}\;(range,\;70{\sim}100^{\circ})$ and $90.7^{\circ}\;(range,\;55{\sim}100^{\circ})$. Over 90% showed more than good results. 30 cases(94%) showed complete bony union but two cases, nonunion. One case of the nonunion cases underwent replating with bone graft as revision surgery and total elbow arthroplasty was performed in the other case. At the last follow-up, 27 patients(84.4%) showed subjective satisfaction. Conclusion: Open reduction and internal fixation with appropriate surgical technique for comminuted fractures of distal humerus showed good results, which were not affected by age at the time of operation, fixation methods and anterior transposition of the ulnar nerve. Transolecranon approach may be considered as good choice for intraarticular comminuted fractures of distal humerus.

• 생명과학회지
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• 제28권3호
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• pp.275-283
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• 2018

인간 섬유아세포 성장인자는 조직 생성 및 상처 치료 효과로 인해 상업적으로 중요한 치료제 또는 화장품소재로서 가능성이 높다. 피부 조직에 침투성을 부여하여 치료효과를 높이기 위해서 단백질 전달 도메인인 PTD를 FGF에 융합을 시도하고 있으며 피부로의 투과능력과 그로인한 치료 효과에 대한 연구도 진행되고 있다. 그러나, PTD가 FGF 단백질의 N- 또는 C-말단에 결합 되었을 때 PTD의 위치가 대장균 발현시스템에서 재조합단백질 접힘 및 안정성, 그리고 결국 피부로의 도입능력에 상당한 영향을 미치는지에 대해서는 알려져 있지 않다. 여기에서 우리는 대조군으로 PTD가 융합되지 않은 인간 염기성 섬유아세포 성장인자(FGF2)와 PTD가 FGF2의 N-말단 또는 C-말단에 융합된 FGF2 복합체를 중합효소연쇄반응(OE-PCR)을 통해 클로닝 하였다. 그 다음 이들 재조합 FGF2의 단백질 발현 및 특성을 확인하고 마우스 등 피부를 이용하여 조직 내로의 도입 능력을 조사 하였다. 결과적으로, 불용성 PTD-FGF2 (N 말단 융합)와는 달리 대조군 FGF2와 FGF2-PTD 융합 단백질(C- 말단 융합)은 가용성 형태로 발현되어 재조합단백질 획득이 용이하였고, 마우스 피부 도입능력은 FGF2-PTD 융합단백질에서만 나타내 보였다. 우리의 결과는 C-말단에 융합된 FGF2-PTD 융합단백질이 발현, 정제, 피부 투과능력의 측면에서 다른 두 옵션들보다 노동, 비용, 시간면에서 보다 더 효율적일 수 있음을 시사한다.