• Title/Summary/Keyword: C-stain

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Scientific Investigation and Conservation of Jocheonillgi (The Dairy of Jocheon) (Treasure No.1007) (보물 제1007호 조천일기(朝天日記)의 과학적 조사와 보존)

  • Ahn, Ji Yoon;Shin, Hyo Young;Son, Mi Kyung;Song, Jung Won
    • Journal of Conservation Science
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    • v.31 no.3
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    • pp.215-225
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    • 2015
  • "Jocheonillgi", one of the articles left by Jo Heon(1544~1592) whose pen name was Jung-bong, is a travelogue which was written in around 1574~1575 when he was dispatched to Ming as a formal envoy(Giljungkwan). The diary-style manuscript was designated as one of the pieces of the National Treasure 1007. Due to the damage of its binding strings, abrasion, fold, stain, insect and damage on the surface, conservation and restoration was needed. In the process of separation, three more binding strings were found, confirming that the travelogue was rebound at least twice in the past. In addition, the page of 'Yeondoillgi', the original title, was bound inside, confirming that the current cover was revised in the past. As the result of the investigation of base fabric, paper mulberry was found to be the cover, inside paper, lining paper, paper strings. The three kinds of binding string including the current ones was defined to be silk.

Effects of Low Power Laser on Pain Response and Axonal Regeneration in Rat Models with Sciatic Nerve Crush Injury

  • Lee, Hong-Gyun;Kim, Yong-Eok;Min, Kyung-Ok;Yoo, Young-Dae;Kim, Kyung-Yoon;Kim, Gye-Yeop
    • Journal of International Academy of Physical Therapy Research
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    • v.3 no.1
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    • pp.345-355
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    • 2012
  • This study purposed to examine the effect of low power laser on pain response and axonal regeneration. In order to prepare peripheral nerve injury models, we crushed the sciatic nerve of Sprague-Dawley rats and treated them with low power laser for 21 days. The rats were divided into 4 groups: normal group(n=10); control group(n=10) without any treatment after the induction of sciatic nerve crush injury; experimental group I(n=10) treated with low power laser(0.21$mJ/mm^2$) after the induction of sciatic nerve crush injury; and experimental group II(n=10) treated with low power laser(5.25$mJ/mm^2$) after the induction of sciatic nerve crush injury. We measured spontaneous pain behavior(paw withdrawal latency test) and mechanical allodynia(von Frey filament test) for evaluating pain behavioral response, and measured the sciatic function index for evaluating the functional recovery of peripheral nerve before the induction of sciatic nerve crush injury and on day 1, 7, 14 and 21 after the induction. After the experiment was completed, changes in the H & E stain and toluidine blue stain were examined histopathologically, and changes in MAG(myelin associated glycoprotein) and c-fos were examined immunohistologically. According to the results of this study, when low power laser was applied to rat models with sciatic nerve crush injury for 21 days and the results were examined through pain behavior evaluation and neurobehavioral, histopathological and immunohistological analyses, low power laser was found to affect pain response and axonal regeneration in both experimental group I and experimental group II. Moreover, the effect on pain response and axonal regeneration was more positive in experimental group I to which output 0.21$mJ/mm^2$ was applied than in experimental group II to which 5.25$mJ/mm^2$ was applied.

A Study on the Development of Large Aluminum Flange using Casting/Forging Process (주조/단조 기술을 이용한 대형 알루미늄 플랜지 개발에 관한 연구)

  • 배원병;왕신일;서명규;조종래
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.905-909
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    • 2001
  • The significance of casting/forging process for reducing the production cost of large components is being noted in these days. This casting/forging process is a method of forging a workpiece preformed by casting into the final shape. In this study, the casting/forging process has been applied in manufacturing a large aluminum flange in order to determine the optimum forging condition of the aluminum flange. The optimum range of forging temperature of Al 5083 was from $420^{\circ}C$ to $450^{\circ}C$. The suitable strain rate was 1.5 $sec^{-1}$. The deformation amount of a preform in a forging process is key role in the mechanical properties of casting/forging products. In order to find the change of mechanical properties according to effective stain of cast aluminum billets, a hot upsetting test were performed with rectangular blocks and then a uniaxial tensile test was performed with specimens cut from the upsetted billets. The tensile strength and the elongation of cast/upsetted aluminum billets were increased largely until the effective strain was 0.7. FE analysis was performed to determine the configurations of cast preform and die for an aluminum flange. In the FE analysis, the forging load-limit was fixed 1500ton for the low equipment cost. The cast preform was designed so that the effective stain around the neck of a flange exceeds 0.7. In the forging experiment for an aluminum flange, it was confirmed that the optimal configuration of the cast preform predicted by FE analysis was very useful. The cast/forged products using designed preform were made perfectly without any defects.

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Development of Semen Transport System for Cryopreservation and Fertility in Bull Sperm (소 정자의 동결 및 수정능력 향상을 위한 정액운반법의 개발)

  • Lee, Sang-Hee;Song, Eun-Ji;Woo, Jea-Seok;Lee, Seung-Hwan;Kang, Hee-Seol;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.37 no.3
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    • pp.97-102
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    • 2013
  • The objective of this study was to develop of semen transport system for cryopreservation and fertility in bull sperm. The ejaculated semen were diluted with Triladyl containing 20% egg-yolk for transportation. Diluted semen was transported by three methods that there were wrapping tissue (Tissue), sinking under $30^{\circ}C$ water (Water) and sinking between warm water and air (Air) methods. Semen was transported within 2 hours in $0.3^{\circ}C$. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk. And frozen-thawed sperm were estimated with SYBR14/PI double stain for viability, FITC-PNA/PI double stain for acrosome reaction analysis and Rhodamine123 double stain for mitochondrial intact assessment. In results, live sperm (SYBR+/PI-) in Air treatment group ($43.3{\pm}4.7%$) was significantly (p<0.05) higher than other treatment groups (Tissue: $16.3{\pm}2.7%$ and Water: $27.5{\pm}3.1%$), dying sperm (SYBR+/PI+) in Air treatment group ($55.6{\pm}4.7%$) was significantly lower than other treatment groups (Tissue: $77.6{\pm}3.2%$ and Water: $67.6{\pm}3.3%$) (p<0.05). Acrosome reaction in Air treatment group ($0.2{\pm}0.1%$) within live sperm (PI negative region) was significantly (p<0.05) lower than other treatment groups (Tissue: $0.7{\pm}0.2%$ and Water: $0.5{\pm}0.1%$), the acrosome reaction in Air treatment group ($28.6{\pm}2.8%$) within all sperm also was significantly lower than other treatment groups (Tissue: $44.2{\pm}1.8%$ and Water: $36.2{\pm}2.0%$) (p<0.05). And mitochondrial intact in Air treatment group within live ($97.1{\pm}0.4%$) and all ($61.9{\pm}3.3%$) sperm were significantly higher than other treatment groups (Tissue: $85.2{\pm}3.3%$, Water: $87.8{\pm}2.9%$ within live sperm and Tissue: $49.28{\pm}3.7%$, Water: $42.0{\pm}3.1%$ within all sperm) (p<0.05). Therefore, we suggest that transportation by sinking method between warm water and air was beneficial to improvement of fertility in frozen-thawed in bull semen.

Chitinimonas naiadis sp. nov., Isolated from a Freshwater River

  • Padakandla, Shalem Raj;Chae, Jong-Chan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1300-1305
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    • 2017
  • A rod shaped, aerobic, Gram-stain-negative, and motile bacterium, strain $AR2^T$, was isolated from a water sample of Yeongsan river, Republic of Korea. Strain $AR2^T$ clustered closely with the members of the genus Chitinimonas and showed the highest 16S rRNA gene sequence similarity with Chitinimonas prasina $LY03^T$ (96.4%), Chitinimonas viridis $HMD2169^T$ (96.4%), Chitinimonas taiwanensis $cf^T$ (96.2%), and Chitinimonas koreensis $R2A43-10^T$ (94.2%). The predominant fatty acids of strain $AR2^T$ were identified to be summed feature 3 (comprising $C_{16:1}{\omega}7c$ and/or $C_{16:1}{\omega}6c$), $C_{16:0}$, and $C_{10:0}3-OH$. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were found to be the major polar lipids. The genomic DNA G+C content was 60.4 mol%. Based on the polyphasic characterization, the isolated strain $AR2^T$ is described as a representative of a novel species in the genus Chitinimonas, for which the name Chitinimonas naiadis sp. nov. (type strain =$AR2^T$ =KCTC $42755^T$ =JCM $31504^T$) is proposed.

Printing of Polyester and Cotton Blends using Diaminoanthraquinone Disperse Dye and Monochlorotriazinyl Reactive Dye Mixtures (디아미노안트라퀴논계 분산염료/모노클로로트리진형 반응염료에 의한 폴리에스테르/면 혼방 직물의 날염)

  • 강숙녀
    • Textile Coloration and Finishing
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    • v.6 no.2
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    • pp.30-39
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    • 1994
  • To study the printing behaviors of Polyester and cotton(P/C) fabrics printed with disperse and reactive dyes, the effects of alkalis on the fixation of reactive dyes and the alkali-stability of disperse dyes in various methods of fixation were examined. The anthraquinone disperse dyes which have diamino derivatives as substituents without hydroxy group, such as C.I. Disperse Violet 1(D.V.1), C.I. Disperse Violet 28(D.V.28) and C.I.Disperse Blue 60(D.B.60) showed good results of fixation without regard to the concentration of NaHCO$_3$. In case of high temperature steaming(HTS) and unsaturated steaming(US)/HTS, D.V. 1 was alkali-stable and effective for P/C printing. A good result was obtained with D.V.1 and C.I.Reactive Orange 13(R.O.13) paste of 4% $K_{2}CO_{3}$. It was found that the unfixed D.V.28 bearing chloro group can hinder the fixation of monochlorotriaxinyl reactive dyes, and D.B.60 made little stain on 100% cotton. In thermosol(Tm), the dye uptake of D.V.1 was not decreased so much, but those of D.V.28 and D.B.60 were greatly decreased.

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Effect of Hwangryunhaedok-tang Extracts on DNCB-induced Allergic Contact Dermatitis (DNCB로 유발된 알레르기성 접촉피부염에 황련해독탕이 미치는 영향)

  • Kim, Bo-Ae;Yang, Jae-Chan;Park, Chan-Ik
    • The Korea Journal of Herbology
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    • v.24 no.2
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    • pp.1-5
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    • 2009
  • Objectives : HRHDT has been known as a useful prescription with antibiotic, anti-inflammatory, antioxidative and immunosuppressive activity. To evaluate anti-inflammatory effect of HRHDT, we treated HRHDT-skin in Balb/c mice model induced contact hypersensitivity. Methods : Contact hypersensitivity, a local inflammatory respinse of skin, was induced by spreading the back skin of Balb/c mice with 1% DNCB. HRHDT-skin was prepared by dissolving 3% 1,3-butylene glycol extract of HRHDT in solution and treated 2 weeks on the back skin. Results: HRHDT-skin significantly reduced TEWL and erythema by 0.4-1% of DNCB treatment compared with control group. HRHDT-skin reduced IgE on serum obtained from blood of DNCB-treated Balb/c mice. Histopathological examination showed that thickening of the epidermis, hyperkeratosis and the infiltration of inflammatory cells were found in Balb/c mice under conventional circumstances. Conclusions : These results showed that HRHDT-skin could be used as a pharmaceutical material with antiinflammatory effects by reducing IgE in contact hypersensitivity dermatitis Balb/c mice by DNCB.

Recrystallization Controlled Deformation of AISI 4140 (AISI 4140 강재의 재결정 제어변형)

  • 조범호
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1999.03b
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    • pp.135-139
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    • 1999
  • The static softening behavior of AISI 4140 could be characterized by the hot torsion test in the temperature ranges of 10$0^{\circ}C$~120$0^{\circ}C$ and strain rate ranges of 0.05/sec~5/sec. Deformation efficiency which was based on dynamic materials model was calculated from flow stress curves obtained continuous deformation. Interrupted deformation was performed with 2 pass deformation in the pass strain ranges of 0.25{{{{ epsilon _p}}}} ~3{{{{ epsilon _p}}}} and interrupted time ranges of 0.5~100sec. The dependences of process variables pass strain ({{{{ epsilon _i}}}}) stain rate ({{{{ {. } atop {$\varepsilon$ } }}}}) temperature (T) and interpass time ({{{{ {t }_{i } }}}}) on static recrystallization (SRX) and metadynamic recrystallization .(MDRX) could be indicidually predicted from the modified Avrami's equations. Comparison of the softening kinetics between MDRX and SRX showed that the rate of MDRX was more rapid than that of SRX for the same deformation variables. Controlled multipass deformations were performed using deformation efficiency static and metadynamic recrystallization of AISI 4140.

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A Study on the Standardization of the Preparation Method for Waxy Rice Nochi (찹쌀 노치 제조법에 관한 연구)

  • 정영선;이혜수
    • Korean journal of food and cookery science
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    • v.7 no.4
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    • pp.9-13
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    • 1991
  • Nochi is a kind of Korean traditional food made from glutinous rice or millet. This study attempted to examine the effects o( the various factors and to clarify the factors which affect the overall eating quality of Noch. The results were summarized as follows. 1. Starch content of waxy rice was 72.4% and IBC was 0.017%. 2. $\alpha$-amylase and $\beta$-amylase activity of malt were 40.13 and 8.94 units respectively. 3. Waxy rice soaked for 3 hours was ground to flour then sifted 20 mesh sieve. The mixture of waxy rice and malt was steamed for 20 minutes. 4. In sensory evaluation on varying the amount of malt and incubation time, the most favorite tendancy of the overall eating quality was at the level of 8hours incubation made by 5% addition of malt. 5. Total sugar contents, reducing sugar contents and the intensities of iodine stain at the incubation temperature of $60^{\circ}C$ were significantly different from those at $50^{\circ}C$.

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The Effect of Dynamic Strain Aging on the High Temperature Plastic Deformation Behaviour of Al-Mg Alloy (Al-Mg 합금의 고온 소성 변형 특성에 미치는 동적 변형 시효의 영향)

  • 이상용;이정환
    • Transactions of Materials Processing
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    • v.5 no.4
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    • pp.327-336
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    • 1996
  • The effect of dynamic strain aging on high temperature deformation behaviour of the A-Mg alloy was investigated by strain rate change tests and stress relaxation tests between 20$0^{\circ}C$and 50$0^{\circ}C$. Yield point, short stress transient and periodic discontinuities on the stress-strain curve were considered as an evidence of the effect of dynamic strain aging. With this criterion two distinct strain rate-temperature regimes could be manifested. Dynamic strain aging was considered to be effective in the high temperature-low strain rate regime, whereas dynamic recovery was a dominant deformation mechanism in the low temperature-high strain rate regime. It was found that dynamic strain aging in the high temperature deformation was governed by the mechcanism of diffusion-controlled, viscous dislocation movement.

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