• Title/Summary/Keyword: C-sequences

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Molecular Phylogenetic Analysis of HIV -1 vif Gene from Korean Isolates

  • Park, Chan-Seung;Kim, Mi-Sook;Lee, Sung-Duk;Kim, Sung-Soo;Lee, Keon-Myung;Lee, Chan-Hee
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.655-659
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    • 2006
  • Phylogenetic studies of nef, pol, and env gene sequences of HIV-1 isolated from Koreans suggested the presence of a Korean clade in which Korean sequences are clustered to the exclusion of foreign sequences. We attempted to identify and characterize the Korean clade using all vif gene sequences isolated from Koreans registered in the NCBI GenBank database (n = 233). Most (77 %) of the Korean isolates belonged to the Korean clade as a large subcluster in subtype B, designated the Korean clade subtype B ($K_{C}B$). $K_{C}B$ sequences were relatively homogenous compared to Korean subtype B sequences that did not belong to the $K_{C}B$ (non-Korean clade subtype B; $NK_{C}B$). Comparison of amino acid frequencies of $K_{C}B$ and $NK_{C}B$ sequences revealed several positions where the amino acid frequencies were significantly different. These amino acid residues were critical in separating $K_{C}B$ from $NK_{C}B$ or from foreign sequences, since substitution of these amino acids in $K_{C}B$ with the $NK_{C}B$ amino acids relocated the $K_{C}B$ sequences to $NK_{C}B$, and vice versa. Further analyses of $K_{C}B$ will help us to understand the origin and evolutionary history of $K_{C}B$.

Bleaching of Oak Kraft Pulp by O, Eo and C/D Stage (O, Eo 및 C/D단계를 이용한 신갈나무 크라프트펄프표백)

  • 김동호;백기현
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.29 no.3
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    • pp.43-50
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    • 1997
  • The purpose of this study was to decrease pollutants of effluents from KP bleaching. Multistage bleaching that combined with O, Eo, and C/D stage was conducted, after optimum bleaching conditions of O, Eo, and C/D stage were determined. Moreover the properties for bleached pulps and pollution level of bleaching effluent were measured by each standard method. Due to increase on the brightness and delignification of pulps by O, Eo, and C/D stage bleaching. It was possible that bleaching sequences were reduced from five stage to four stage. In addition to, OC/DEoD and OC/DEopD bleaching sequences had higher brightness than CEDED bleaching sequence. The pulps bleached by O and Eo stage had higher in tensile index than conventional bleaching sequences with the similar results in burst and tear index. While each bleaching sequences had little differences, bleaching sequences with Eo stage had higher in physical properties than with Eop stage. The pollution level measured by Chemical Oxygen Demand(COD), Biochemical Oxygen Demand(BOD) and Color of bleaching effluents were much lower in combined bleaching sequences of O, Eo and C/D stage than that of CEDED sequence. Particularly, a pollution index of bleaching effluents generated by C/DEoDEB and OC/DEopDED bleaching sequences were lower than that from the other bleaching sequences.

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Genetic Distinctness of Sorex caecutiens hallamontanus (Soricomorpha: Mammalia) from Jeju Island in Korea: Cytochrome Oxidase I and Cytochrome b Sequence Analyses

  • Koh, Hung-Sun;Jang, Kyung-Hee;In, Seong-Teak;Han, Eui-Dong;Jo, Jae-Eun;Ham, Eui-Jeong;Jeong, Seon-Ki;Lee, Jong-Hyek;Kim, Kwang-Seon;Kweon, Gu-Hee
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.215-219
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    • 2012
  • To examine genetic divergences of two endemic Sorex caecutiens subspecies from Korea (S. c. hallamontanus in Korean Jeju Island and S. c. annexus in the mainland Korean Peninsula), we obtained partial cytochrome oxidase I (COI) sequences (429 bp) and complete cytochrome b sequences (1,140 bp) from the two Korean subspecies, and we compared these sequences to the corresponding sequences of S. caecutiens, obtained from GenBank. We found that Jeju S. c. hallamontanus is one of three clades within S. caecutiens, with an average Jukes-Cantor distance of 1.57% in the COI sequences and the distance of 2.07% and 11 fixed site differences in the cytochrome b sequences, indicating that Jeju S. c. hallamontanus is one endemic subspecies with concordant genetic distinctness, although further analyses with nuclear DNA sequences are necessary to confirm these findings. However, S. c. annexus from the mainland Korean Peninsula was not divergent from S. c. macropygmaeus from northeastern China and adjacent Russia, indicating that S. c. annexus from the mainland Korean Peninsula is another endemic subspecies with only morphological differences, although it is necessary to reexamine the subspecies status of S. c. annexus.

Analysis of Partial cDNA Sequence from Human Fetal Liver

  • Kim, Jae-Wha;Song, Jae-Chan;Lee, In-Ae;Lee, Young-Hee;Nam, Myoung-Soo;Hahn, Yoon-Soo;Chung, Jae-Hoon;Choe, In-Seong
    • BMB Reports
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    • v.28 no.5
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    • pp.402-407
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    • 1995
  • Single-run Partial cDNA sequencing was conducted on 1,592 randomly selected human fetal liver cDNA clones of Korean origin to isolate novel genes related to liver functions. Each partial cDNA sequence determined was analyzed by comparing it with the databases. GenBank, Protein Information Resource (PIR) and SWISS-PROT Protein Sequence Data Bank. From a set of 1.592 cDNA clones reported here, 1,433 (90.0% of the total) were informative cDNA sequences. The other 159 clones were identified as DNA sequences which had originated from the cloning vector. Among 1,433 informative partial cDNA sequences, 851 (59.3%) clones were revealed to be identical to known human genes. These known genes have been classified into 225 different kinds of genes. In addition, 340 clones (23.7%) showed various degrees of homology to previously known human genes. Ninety four (6.6%) clones contained various repeated sequences. Twenty four (1.7%) partial cDNA sequences were found to have considerable homology to known genes from evolutionarily distant organism such as yeast, rice, Arabidopsis, mouse and rat, based on database matches, whereas 124 (8.7%) had no Significant matches. Human homologues to functionally characterized genes from different organisms could be classified as candidates for novel human genes of similar functions. Information from the partial cDNA sequences in this study may facilitate the analysis of genes expressed in human fetal liver.

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ESSENTIAL EXACT SEQUENCES

  • Akray, Ismael;Zebari, Amin
    • Communications of the Korean Mathematical Society
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    • v.35 no.2
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    • pp.469-480
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    • 2020
  • Let R be a commutative ring with identity and M a unital R-module. We give a new generalization of exact sequences called e-exact sequences. A sequence $0{\rightarrow}A{\longrightarrow[20]^f}B{\longrightarrow[20]^g}C{\rightarrow}0$ is said to be e-exact if f is monic, Imf ≤e Kerg and Img ≤e C. We modify many famous theorems including exact sequences to one includes e-exact sequences like 3 × 3 lemma, four and five lemmas. Next, we prove that for torsion-free module M, the contravariant functor Hom(-, M) is left e-exact and the covariant functor M ⊗ - is right e-exact. Finally, we define e-projective module and characterize it. We show that the direct sum of R-modules is e-projective module if and only if each summand is e-projective.

Characterization of the Folding Structure of 3'-end of Lactate Dehydrogenase A-mRNA Isolated from Hormone Stimulated Rat $C_{6}$ Glioma cell culture (홀몬으로 처리된 쥐의 $C_{6}$ glioma 세포배양으로부터 분리된 낙산탈수소 효소 A-mRNA의 3'-말단의 2차 구조)

  • 배석철;이승기
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.94-102
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    • 1987
  • Rat liver LDH A-cDNA has been isolated from a .lambda.gt11-rat lover cDNA library and partially characterized. The size of the isolated rat liver LDH A-cDNA if shown to be 1.6Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 682-nucleotide sequence coding for 3'-end of rat liver LDH A-cDNA has been analyzed and compared to the nucleotide sequence of the same region of rat $C_6$-glioma cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terminal 59-amino acids are identical but 505 nucleotide sequences of 3'-nontranslated region of the two LSH A-cDNA exhibit characteristic differences in thier nucleotide sequences. Computer analysis for the folding structures for 3'-end 400 nucleotide sequences of the two LDH A-cDNA shows a possibility implying that the two LDH A-mRNAs isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different. It has been previously demonstrated that isoproterenol stimulated rat $C_6$ -glioma cell cultures produce LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of noninduced LHD A-mRNA. The result therefore support for the idea that hormonally stimulated rat $C_6$-glioma cells may produce LDH A-mRNA containing different nucleotide sequences at the 3'-end nontranslated region by which the hormonally induced LDH A-mRNA could have more stable secondary mRAN structure in comparison to that of noninduced LDH A-mRNA.

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A CHARACTERIZATION OF THE RIORDAN BELL SUBGROUP BY C-SEQUENCES

  • Jin, Sung-Tae
    • Korean Journal of Mathematics
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    • v.17 no.2
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    • pp.147-154
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    • 2009
  • In this paper, we consider a new sequence called by the C-sequence of the Riordan array. It allows us to find a simple proof for several combinatorial identities. Further, we prove that a C-sequence characterizes Bell subgroup of the Riordan group.

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Cloning and Characterization of Highly Repetitive Sequences in the Genome of Allium sativum L. (마늘(Allium sativum L.) 게놈의 고반복서열의 분이와 특성 조사)

  • 이동희
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.49-55
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    • 1996
  • We have studied the DNA of Allium sativum L. with respect to highly repetitive sequences. Fast reassociated DNA fragments expected to be highly repetitive sequences based on $C_{o}t$ curve were isolated and characterized. Their copy numbers were approximately $10^{5}~10^{7}$ per haploid genome. Nucleotide sequences analysis of six candidates reveals that their G/C content were low, 25-40% and typical patterns of repeating sequences exist. Repeat sequences were used as probes to access restriction fragment length polymorphism (RFLP) of genomic DNAs of four local clones, Tanyang, Mungyong, So san, and Uisong. The hybridization pattern were very similar among these four local clones.clones.

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ON "VERY PALINDROMIC" SEQUENCES

  • BASIC, BOJAN
    • Journal of the Korean Mathematical Society
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    • v.52 no.4
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    • pp.765-780
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    • 2015
  • We consider the problem of characterizing the palindromic sequences ${\langle}c_{d-1},\;c_{d-2}\;,{\cdots},\;c_0\rangle$, $c_{d-1}{\neq}0$, having the property that for any $K{\in}\mathbb{N}$ there exists a number that is a palindrome simultaneously in K different bases, with ${\langle}c_{d-1},\;c_{d-2}\;,{\cdots},\;c_0\rangle$ being its digit sequence in one of those bases. Since each number is trivially a palindrome in all bases greater than itself, we impose the restriction that only palindromes with at least two digits are taken into account. We further consider a related problem, where we count only palindromes with a fixed number of digits (that is, d). The first problem turns out not to be very hard; we show that all the palindromic sequences have the required property, even with the additional point that we can actually restrict the counted palindromes to have at least d digits. The second one is quite tougher; we show that all the palindromic sequences of length d = 3 have the required property (and the same holds for d = 2, based on some earlier results), while for larger values of d we present some arguments showing that this tendency is quite likely to change.