• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.45 no.5
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• pp.14-22
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• 2013

In this study, we investigated retention characteristics of nanocoated GCC that was positively modified by Layer-by-Layer (LbL) multilayering process. Three layers were formed onto GCC particles with poly-DADMAC/PSS/poly-DADMAC (PD3) and C-starch/A-PAM/C-starch (CS3) systems, respectively. Untreated GCC, PD3 GCC (strongly positive charge) and CS3 GCC (weekly positive charge) were retained on pulp fibers under single retention system or microparticle retention system conditions. In single retention system, PD3 particles were not affected by cationic retention aid due to their strong positive charge, whereas CS3 particles reacted with cationic retention aid due to anionic sites on the surface of the weekly positive particles. In a microparticle retention system, positively modified GCC (PD3 and CS3) showed higher retention level than untreated GCC at the same dosage of retention aid. The cationic surface of GCC particles were more reacted with bentonite so the deposition onto pulp fibers was improved. In addition, the retention level of nanocoated GCC was increased with maintaining good formation.

• ALGAE
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• v.27 no.2
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• pp.125-134
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• 2012

Germanium dioxide ($GeO_2$) has been used for many years in the cultivation of red and green algae as a means of controlling the growth of diatoms. Brown algae are sensitive to $GeO_2$, however, the basis of this sensitivity has not been characterized. Here we use embryos of $Fucus$ $vesiculosus$ to investigate morphological and physiological impacts of $GeO_2$ toxicity. Morphometric features of embryos were measured microscopically, and physiological features were determined using pulse amplitude modulated (PAM) fluorometry. At 5 mg $L^{-1}$ $GeO_2$, embryos grew slower than controls and developed growth abnormalities. After 24 h, initial zygote divisions were often oblique rather than transverse. Rhizoids had inflated tips in $GeO_2$ and were less branched, and apical hairs were deformed, with irregularly aligned, spheroidal cells. Minimum fluorescence ($F_0$) showed minor differences over the 10 days experiment, and pigment levels (chlorophylls $a$, $c$ and total carotenoids) showed no difference after 10 days. Optimum quantum yield increased from ca. 0.52 at 24 h to 0.67 at 5 days, and $GeO_2$-treated embryos had higher mean values (significant at 3 and 5 days). Optimum quantum yield of photosystem II (${\Phi}_{PSII}$) was stable in control thalli after 5 days, but declined significantly in $GeO_2$. Addition of silica (as $SiO_2$) did not reverse the effects of $GeO_2$. These results suggest that $GeO_2$ toxicity in brown algae is associated with negative impacts at the cytological level rather than metabolic impacts associated with photosynthesis.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06a
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• pp.61-66
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• 2006

Over the past 20 years there has been a revolution involving the use of nano or macro size particles as drainage and retention systems during the manufacture of paper. More recently a group of patented technologies called Synthetic Mineral Microparticles (SMM) has been invented and developed. This system has potential to further promote the drainage of water and retention of fine particles during papermaking. Prior research, as well as our on preliminary research showed that the SMM system has advantages in both of drainage and retention compared with montmorillonite (bentonite), which one of the most popular materials presently used in this kind of application. In spite of the demonstrated advantages of this SMM system, the properties and activity of SMM particles in the aqueous state have not been elucidated yet. Streaming current titrations with highly charged polyelectrolytes were used to measure the charge properties of SMM and to understand the interactions among SMM particles, fibers, fiber fines, and cationic polyacrylamide (cPAM) as a retention aid. It was found that pH profoundly affects the charge properties of SMM, due to the influence of Al-ions and the Si-containing particle surface. SEM pictures, characterizing the morphology, geometry and size distribution of SMM, showed an broad distribution of primary particle size. Dilution of SMM mixturee appeared to wash out particles smaller than 100 nm from the surface of larger particles, which themselves appeared to be composed of fused primary particles. DSC thermoporometry was used to measure the size distribution of nanopores within SMM particles.

• Biomolecules & Therapeutics
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• v.26 no.5
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• pp.481-486
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• 2018

Cosmetics are primarily applied to the skin; therefore, the association of cosmetic dyes with skin diseases or inflammation is a topic of great interest. Thymic stromal lymphopoietin (TSLP) is an interleukin 7-like cytokine that activates dendritic cells to promote Th2 inflammatory immune responses. TSLP is highly expressed in keratinocytes under inflammatory conditions, which suggests that it may play a critical role in the development of skin diseases, such as atopic dermatitis. Therefore, we investigated whether cosmetic dyes influenced the production of TSLP by keratinocytes. Phloxine O, also known as D&C Red No.27, is one of the most common red synthetic pigments and is widely used in colored cosmetics. Our results showed that Phloxine O downregulated phorbol 12-myristate 13-acetate-induced production of TSLP in a murine keratinocyte cell line (PAM212). Phloxine O also suppressed TSLP expression in KCMH-1 cells, which are mouse keratinocytes that constitutively produce high levels of TSLP. To investigate the in vivo effects of Phloxine O, we induced TSLP expression in mouse ear skin by topically applying MC903, a vitamin D3 analogue that is a well-known inducer of atopic dermatitis-like symptoms. Topical application of Phloxine O prevented MC903-induced TSLP production in mouse ear skin, attenuated the acute dermatitis-like symptoms and decreased serum IgE and histamine levels in mice. Suppression of TSLP expression by Phloxine O correlated with reduced expression of OX40 ligand and Th2 cytokines in mouse ear skin. Our results showed that Phloxine O may be beneficial to prevent dermatitis by suppressing the expression of TSLP and Th2 cytokines in skin.

• Journal of Sensor Science and Technology
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• v.15 no.1
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• pp.58-64
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• 2006

In this study, we describe a low-temperature wafer-level thermocompression bonding using electroplated gold seal line and bonding pads by electroplating method for RF-MEMS devices. Silicon wafers, electroplated with gold (Au), were completely bonded at $320^{\circ}C$ for 30 min at a pressure of 2.5 MPa. The through-hole interconnection between the packaged devices and external terminal did not need metal filling process and was made by gold films deposited on the sidewall of the throughhole. This process was low-cost and short in duration. Helium leak rate, which is measured to evaluate the reliability of bonded wafers, was $2.7{\pm}0.614{\times}10^{-10}Pam^{3}/s$. The insertion loss of the CPW packaged was $-0.069{\sim}-0.085\;dB$. The difference of the insertion loss between the unpackaged and packaged CPW was less than -0.03. These values show very good RF characteristics of the packaging. Therefore, gold thermocompression bonding can be applied to high quality hermetic wafer level packaging of RF-MEMS devices.

• Fisheries and Aquatic Sciences
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• v.12 no.1
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• pp.70-77
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• 2009

Porphyra yezoensis is known to act as a biofilter against nutrient-rich effluent in seaweed-based integrated aquaculture systems. However, few studies have examined its physiological status under such conditions. In this study, we estimated the photosynthetic activity of P. yezoensis by chlorophyll fluorescence of PSII (${\Delta}F/F'm$ and relative $ETR_{max}$) using the Diving-PAM fluorometer (Walz, Germany). In addition, bioremediation capacity, tissue nutrients, and C:N ratio of P. yezoensis were investigated. The ammonium concentration in seawater of seaweed tank 4 decreased from $72.1{\pm}2.2$ to $33.8{\pm}0.4{\mu}M$ after 24 hours. This indicates the potential role of P. yezoensis in removing around 43% of ammonium from the effluents. Tissue carbon contents in P. yezoensis were constant during the experimental period, while nitrogen contents had increased slightly by 24 hours. In comparison with the initial values, the ${\Delta}F/F'm$ and $rETR_{max}$ of P. yezoensis had increased by about 20 and 40%, respectively, after 24 hours. This indicates that P. yezoensis condition improved or remained constant. These results suggest that chlorophyll fluorescence is a powerful tool in evaluating the physiological status of seaweeds in a seaweed-based integrated aquaculture system.

• Genomics & Informatics
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• v.4 no.3
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• pp.97-102
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• 2006

In acute leukemia patients, several successful methods of expression profiling have been used for various purposes, i.e., to identify new disease class, to select a therapeutic target, or to predict chemo-sensitivity and clinical outcome. In the present study, we tested the peripheral blood of 47 acute leukemia patients in an attempt to identify differentially expressed genes in AML and ALL using a Korean-made 10K oligo-nucleotide microarray. Methods: Total RNA was prepared from peripheral blood and amplified for microarray experimentation. SAM (significant analysis of microarray) and PAM (prediction analysis of microarray) were used to select significant genes. The selected genes were tested for in a test group, independently of the training group. Results: We identified 345 differentially expressed genes that differentiated AML and ALL patients (FWER<0.05). Genes were selected using the training group (n=35) and tested for in the test group (n=12). Both training group and test group discriminated AML and ALL patients accurately. Genes that showed relatively high expression in AML patients were deoxynucleotidyl transferase, pre-B lymphocyte gene 3, B-cell linker, CD9 antigen, lymphoid enhancer-binding factor 1, CD79B antigen, and early B-cell factor. Genes highly expressed in ALL patients were annexin A 1, amyloid beta (A4) precursor protein, amyloid beta (A4) precursor-like protein 2, cathepsin C, lysozyme (renal amyloidosis), myeloperoxidase, and hematopoietic prostaglandin D2 synthase. Conclusion: This study provided genome wide molecular signatures of Korean acute leukemia patients, which clearly identify AML and ALL. Given with other reported signatures, these molecular signatures provide a means of achieving a molecular diagnosis in Korean acute leukemia patents.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.7
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• pp.711-716
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• 2012

The purpose of this study was to investigate how the modulation method affects the effectiveness of eliciting tactile sensations by electrical stimulation. Two methods were employed and the results were compared and analyzed; pulse amplitude modulation (PAM) and pulse width modulation (PWM). Thirty-five healthy subjects participated in the experiments to measure the stimulation intensity that began to elicit a tactile sensation - activation threshold (AT). Constant-current monophasic rectangular pulse trains were employed, and the stimulation intensity was varied from zero until the subject felt any uncomfortable sensation. The step size of the stimulation intensity was 100nC/pulse. After each experiment, the subject described the sensation both quantitatively and qualitatively. The two modulation methods did not make a significant difference as far as the AT values were concerned, but most of the subjects showed 'intra-individual' consistency. Also, it was confirmed that our range of the stimulation parameters enabled us to obtain three major tactile sensations; tickling, pressure and vibration. The results suggested that the stimulation parameters and the modulation type should be selected for each individual and that selective electrical stimulation of the mechanoreceptors needs more diversified researches on the electrode design, multi-channel stimulation protocol, waveforms of the pulse train, etc.

• Molecules and Cells
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• v.43 no.10
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• pp.870-879
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• 2020

Dendrites require precise and timely delivery of protein substrates to distal areas to ensure the correct morphology and function of neurons. Many of these protein substrates are supplied in the form of ribonucleoprotein (RNP) complex consisting of RNA-binding proteins (RBPs) and mRNAs, which are subsequently translated in distal dendritic areas. It remains elusive, however, whether key RBPs supply mRNA according to local demands individually or in a coordinated manner. In this study, we investigated how Drosophila sensory neurons respond to the dysregulation of a disease-associated RBP, Ataxin-2 (ATX2), which leads to dendritic defects. We found that ATX2 plays a crucial role in spacing dendritic branches for the optimal dendritic receptive fields in Drosophila class IV dendritic arborization (C4da) neurons, where both expression level and subcellular location of ATX2 contribute significantly to this effect. We showed that translational upregulation through the expression of eukaryotic translation initiation factor 4E (eIF4E) further enhanced the ATX2-induced dendritic phenotypes. Additionally, we found that the expression level of another disease-associated RBP, fragile X mental retardation protein (FMRP), decreased in both cell bodies and dendrites when neurons were faced with aberrant upregulation of ATX2. Finally, we revealed that the PAM2 motif of ATX2, which mediates its interaction with poly(A)-binding protein (PABP), is potentially necessary for the decrease of FMRP in certain neuronal stress conditions. Collectively, our data suggest that dysregulation of RBPs triggers a compensatory regulation of other functionally-overlapping RBPs to minimize RBP dysregulation-associated aberrations that hinder neuronal homeostasis in dendrites.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.1098-1099
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• 2015

${\bullet}$ 시험대상: 영월태양광 4,990kW(998kW ${\times}$ 5호) ${\bullet}$ 13.07.31 연계기술검토의뢰 ${\bullet}$ 13.08.28 한전검토결과: 인근변전소 영월S/S(40MW) 초과로 10.8km 지점의 평창 D/L 평창간 347호 주진S/S에 다음의 보완대책과 연계 권고 ${\cdot}$ 문제점: 적정전압이탈 및 순시전압변동률 유지불가 ${\cdot}$ 연계기준만족을 위해 한전에서 제시한 보완대책 : 계통용량증설(설비보강), 연계용량 감소 및 전용선로 연계 ${\bullet}$ 14.01.09 분산형설치자의 용역사(에이스기술단)에서 송배전용전기설비 이용규정과 연계기준 만족을 위한 보완대책 제안 ${\cdot}$ 태양광발전시스템 인버터 자동전압(역률/무효전력) 조정기능 및 감시설비 구비 ${\cdot}$ 최적 공통연결점 선정: 분산형전원으로 부터 전기적으로 가장 가까운 영월D/L 영월S/S측에 개폐장치설치 하고 주진S/S 측으로 연계 ${\bullet}$ 14.01.29~06.19 한전(전력연구원)의 유효성 검토 ${\cdot}$ 보완대책 기술적 유효(실증시험 시행 후 시행공문 발송 예정) ${\bullet}$ 14.08.08 송배전용전기설비 이용규정 개정에 따른 협의 ${\cdot}$ 변압기 대당 20MW에서 25MW ${\cdot}$ 변전소 전체 60MW에서 75MW까지 허용 ${\cdot}$ 영월 S/S 측 문곡/영월 D/L 연계 공사 협의 ${\bullet}$ 14.10.04 사용전검사, 한전계통연계운전 시작 ${\bullet}$ 인버터 고정역률(99.9~100%) 운전 시험 ${\cdot}$ 14.11.21 22.9kV 계전기(SEL-751) 5대, 15분 간격 자료저장 ${\cdot}$ SEL-751/USB to RS232C/노트북 15분간격 측정(첨부) ${\bullet}$ 14.03.07 인버터 역률 95.0% 조정 15분간격 측정(첨부) ${\bullet}$ 변압기탭 선정과 인버터전압조정협조시험 계획 ${\cdot}$ 변압기탭을 현장에서 상하 1탭씩 변경 ${\cdot}$ 인버터역률을 인버터공급사에서 원격조정 ${\cdot}$ 22.9kV 보호계전기 SEL-751, 15분 간격 측정 ${\cdot}$ 인버터 370V 계전기 K-PAM DG3000/현장감시설비 연계 ${\cdot}$ 15분 간격 측정 저장 ${\cdot}$ 현장감시설비/인터넷 연결 원격측정(첨부예정)