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Enzymatic Method for Measuring ATP Related Compounds in Fish Sauces (효소법에 의한 액젓중의 ATP 관련물질 측정)

  • CHO Young Je;IM Yeong Sun;KIM Sang Moo;CHOI Young Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.385-390
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    • 1999
  • HPLC method usually has been used for the determination of ATP and its related compounds in fish muscle and fish sauce. But, total amount of ATP related compounds in fish sauce is determined less than that of fish muscle. In order to establish the extract analysis method for ATP related compounds in fish sauce, a new enzymatic method was developed and compared with existing HPLC method. Fish sauce was extracted with chilled perchloric acid and neutralized to Ph 7.0 with potassium hydroxide solution, the extract was used as sample analyzed by HPLC as usual. On the other hand, for sample analyzed by enzymatic method, 1 ml extract solution was pipetted into test tube. To the tube, 0.5ml of mixed suspension adenosinedeaminase (4U), nucleosidephosphorylase (0.02U) and xanthineoxidase (0.03U) suspended in 2.0ml of 1/15 M sodium phosphate buffer solution pH 7.6 and 1.5ml deionized water wereadded for the decomposition of IMP, HxR and Hx to uric acid at $37^{\circ}C$ for 40 minutes. Total uric acid was determined by measuring optical density at 290nm. In HPLC method, salt decreased the total amount of ATP related compounds by $13.6\~16.2\%$ at $2.5\%$ concentration, but no effect in enzymatic method. IMP, HxR and Hx were detected at 254nm, while uric acid at only 290nm. The ratio of the total amount of ATP related compounds by HPLC method was about $45\%$ of that by enzymatic method in fish sauce. Form these results, enzymatic method is more accurate and simple than HPLC method for analysis of ATP related compounds in fish sauce.

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A Phytosociological Study of the Forest Communities in Mt. Palgong(I) -Pinus densiflora Forests- (팔공산(八公山) 삼림군락(森林群落)의 식물사회학적연구(植物社會學的硏究)(I) -소나무림(林)에 대해서-)

  • Cho, Hyun Je;Hong, Sung Cheon
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.144-161
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    • 1990
  • Pine forest communities developed in Mt. Palgong, southeastern Korea, were studied phytosociologically, with special reference to multiple management of local forests, and were classified into two communities, Pinus densiflora - Quercus mongolica community(I : mountain forest) and P. densiflora-Climbing plants community (II : valley forest) and six subgroups accompanied by several subgroups. Judging from the coincidence method, the division of communities (vegetation units) was closely related to altitude and topography. Based on vegetation units, a vertical distribution map of pine forest communities was prepared. The species composition(%) of pine forest communities under stratification, in upper and lower tree layer, teas I higher than II, in middle and shrubs lacer II higher than I (Total : Upper 15.5%, Middle 28.4%, Lower 34.6%. Shrubs 21.5%. Sum of mean coverage%i of understory vegetation was II twice as high as I (Total. shrubs 28.4%. forbs 11.4%, Graminoids 11.8%, ferns 1.0%). Based on constance, coverage and d.b.h. class etc., an actual growth and occurrence table of tree species and understory vegetation by vegetation unity were prepared, and could assume a criterion for judging potential dominance-growth conditions.

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A Study on the Health Status of Dental Technicians by Todai Health Index(THI) (THI에 의한 일부 치과기공사의 건강상태에 관한 조사 연구)

  • Kwon, Soon-Suk;Moon, Hee-Jung;Shin, Myung-Suk;Kim, Yoon-Shin
    • Journal of dental hygiene science
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    • v.9 no.2
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    • pp.169-179
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    • 2009
  • This study aims to present a fundamental data base to figure out the mental and the physical conditions that the dental technicians are facing and ultimately to develop a health care program to deal with their health related problems. To this end, we took an analysis on the health status among the subjects of 895 dental technicians currently working at the dental lab around the nation from January 15 to March 31, 2009 by way or Todai Health Index(THI). Of the average scale point in accordance with 12 scale scores of the physical and the mental subjective symptom, the results revealed that the physical appeals (21.10) were higher than the mental appeals (18.49) and the multiple subjective symptom was marked as 38.44 followed by the mental irritability (25.92). In gender differences, the females proved to be higher than the males in both physical appeals and mental appeals while the physical appeals were dominant in both genders. The physical appeals were higher than the mental appeals with regard to the general characteristics. In the case or the group or age twenties as shown in the physical and mental average scale point, the other groups showed 21.55% of the physical appeals among the married whereas the mental appeals showed the highest point as 18.70 in the unmarried group. In job position, the other groups marked the highest, in working condition, below average group marked the highest, in frequency of break time, none group marked the highest. We drew a conclusion form this study that the dental technicians gained the higher points in the item or the multiple subjective symptom, the menial irritability, and the irregular life. More research on th is phenomena should be followed along with the development of various and practical health care programs to promote the health or dental technicians.

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Performance Analysis of Photovoltaic System for Greenhouse (태양광 발전시스템의 발전 성능 분석)

  • Kwon, Sun-Ju;Min, Young-Bong;Choi, Jin-Sik;Yoon, Yong-Cheol
    • Journal of agriculture & life science
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    • v.46 no.5
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    • pp.143-152
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    • 2012
  • This study was performed to reduce the operating cost of a greenhouse by securing electric energy required for greenhouse operation. Therefore, it experimentally reviewed the performance analysis of photovoltaic system in terms of maximum amount of generated electric power based on the amount of horizontal solar radiation during daytime. That is to say, the maximum solar radiation at 300, 400, 500, 600, 700, 800 and 900 W. $m^{-2}$, respectively. The amount of momentary electric power of the photovoltaic system at any was about 970 W and we found that the momentary efficiency of the photovoltaic system that was used for this experiment was 97%. In the case of this system, we found that electric power will be generated when amount of horizontal solar radiation is more than 200 W. $m^{-2}$, at minimum. If the amount of horizontal solar radiation is increased, the maximum power generation is also increased. At that time, the maximum efficiencies were 30, 78, 86 and 90%, respectively. However, when the amount of insolation was about 800 W. $m^{-2}$, the maximum power generation tended to be lower than 700 W. $m^{-2}$. The efficiency which caused the maximum electric power was decreased to less than 97% of the momentary generated electric power. When the total amounts of horizontal solar radiation per day were 3.24, 8.10, 10, 90, 12.70, 14.33, 19.53 and $21.48MJ{\cdot}m^{-2}$ respectively, the total amounts of power energy were 0.03, 0.40, 3.60, 4.37, 4.71, 4.70 and 4.91 kWh. And it represented that the total amounts of power energy were either decreased or increased a bit on the border between some solar radiations. The temperature at the back of the array tended to be higher than the temperature at the front but it demonstrated an increased when the amount of solar radiation increased. In the case of this system, the performance of the module in terms of degradation has not been shown yet.

Effect of Supplying Volume and Frequency of Nutrient Solution on Growth and Fruit Quality of Blueberry (블루베리 양액재배시 공급량 및 공급횟수가 수체생육 및 과실품질에 미치는 영향)

  • Cheon, Mi Geon;Lee, Young Suk;Chung, Yong Mo;Kim, Hee Dae;Hong, Kwang Pyo;Kumarihami, H.M. Prathibhani C.;Kim, Jin Gook
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.447-453
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    • 2019
  • In this study, the effect of supplying volume and frequency of a nutrient solution consisted with $NO_3-N$ 4.6, $NH_4-N$ 3.4, $PO_4-P$ 3, K 3, Ca 4.6 and Mg $2.2mmol{\cdot}L^{-1}$ on growth and fruit quality of 'Duke' blueberry was investigated. Three years old 'Duke' blueberry bushes cultivated in containers ($60{\times}80{\times}40cm$) filled with 130L peat moss and 40L pearlite (v/v) were selected for the experiment. The growth containers were mulched with sawdust. Two different volumes (4L and 8L) of nutrient solution was tested at three different supplying frequencies (one time, two times, and three times) per week and the drainage quality of nutrient solution and fruit quality of 'Duke' blueberry was evaluated. The optimal drainage rate for the vegetable cultivation is known to be 20-30%. The results revealed that the average drainage rates of 27% and 29% for the nutrient solution supplied in 'Duke' blueberry growth medium at 4L, 2 times/7 days and 4L, 3 times/7days, respectively. The highest shoot diameter (4.2mm) and shoot length (31cm) of 'Duke' blueberry was recorded with the 8L of nutrient solution supplied at 3 times per 7 days. According to the analysis of inorganic components in the drainage of nutrient solution, there was a tendency of absorbing nitrogen at the early stage of growth. The supplying volume and frequency of nutrient solution was not significantly affected on 'Duke' blueberry fruit weight, soluble solids content, and titratable acidity. The highest yield per bush (2.7kg) was recorded for the nutrient solution supplied with 4L at three times per 7 days, while the 4L nutrient solution supplied at one time per 7 days resulted the lowest yield of 1.4kg per bush. Consequently, the tested nutrient solution can be applied for the 'Duke' blueberry bushes with the volume of 4L at three times per week for the better crop growth.

Methods of Incorporating Design for Production Considerations into Concept Design Investigations (개념설계 단계에서 총 건조비를 최소로 하는 생산지향적 설계 적용 방법)

  • H.S.,Bong
    • Bulletin of the Society of Naval Architects of Korea
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    • v.27 no.3
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    • pp.131-136
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    • 1990
  • 여러해 전부터 선박의 생산실적이나 생산성 관련 자료를 기록하고 보완하는 작업을 꾸준히 개선토록 노력해온 결과중 중요한 것 하나는, 선박의 여러 가지 설계 검토과정에서 충분히 활용할 수 있는 함축성 있고 믿을만한 형태의 생산정보를 제공해줄 수 있게 되었다는 것이라고 말 할 수 있겠다. 이러한 자료들은 생산계획상 각 단계(stage)에서의 작업량, 예상재료비와 인건비의 산출등이 포함될 수 있으며, 선박이나 해상구조물의 전반적인 설계방법론(design methodology)을 개선코자 한다면 ''생산지향적 설계(Design for Production)''의 근간이 되는 선박건조전략(build strategy), 구매정책(purchasing policy)과 생산기술(production technology)에 대한 폭넓은 지식이 한데 어우러져야 한다. 최근에는 CIMS의 일부분에서 보는 바와 같은 경영관리, 설계 및 생산지원 시스템의 도입으로 이와 같은 설계 프로세스의 추진을 가능케하고 있다. 이와 병행하여 설계를 지원하기 위한 전산기술, 특히 대화형 화상처리기술(interactive graphics)의 발달은 설계자가 선박의 형상이나 구조 배치를 여러 가지로 변화시켜 가면서 눈으로 즉시 확인할 수 있도록 설계자의 능력을 배가시키는데 크게 기여하고 있다. 여러 가지의 설계안(alternative design arrangement)을 신속히 만들어내고 이를 즉시 검토 평가할 수 있는 능력을 초기설계 단계에서 가질 수 있다면 이는 분명히 큰 장점일 것이며, 더구나 설계초기 단계에 생산관련인자를 설계에서 고려할 수 있다면 이는 더욱 두드러진 발전일 것이다. 생산공법과 관련생산 비용을 정확히 반영한 각 가지의 설계안을 짧은 시간내에 검토하고 생산소요 비용을 산출하여 비교함으로써, 수주계약단계에서 실제적인 생산공법과 신뢰성있는 생산실적자료를 기준으로 하여 총 건조비(total production cost)를 최소로 하는 최적의 설계를 선택할 수 있도록 해 줄 것이다. 이제 이와 같은 새로운 설계도구(design tool)를 제공해 주므로써 초기설계에 각종 생산관련 정보나 지식 및 실적자료가 반영가능토록 발전되었다. 본 논문은 영국의 뉴카슬대학교(Univ. of Newcastle upon Type)에서 위에 언급한 특징들을 반영하여 새로운 선박구조 설계 방법을 개발한 연구결과를 보여주고 있다. 본 선계연구는 5단계로 구분되는데; (1) 컴퓨터 그라픽스를 이용하고 생산정보 데이타베이스와 연결시켜 구조형상(geometry)을 정의하고 구조부재 칫수(scantling) 계산/결정 (2) 블럭 분할(block division) 및 강재 배치(panel arrangement)의 확정을 위해 생산기술 및 건조방식에 대한 정보 제공 (3) 상기 (1) 및 (2)를 활용하여 아래 각 생산 단계에서의 생산작업 분석(work content assessment) a) 생산 준비 단계(Preparation) b) 가공 조립 단계(Fabrication/Assembly) c) 탑재 단계(Erection) (4) 각각의 설계(안)에 대하여 재료비(material cost), 인건비(labour cost) 및 오버헤드 비용(overhead cost)을 산출키 위한 조선소의 생산시설 및 각종 품셈 정보 (5) 총 건조 비용(total production cost)을 산출하여 각각의 설계안을 비교 검토. 본 설계 방식을 산적화물선(Bulk Carrier) 설계에 적용하여 구조배치(structural geometry), 표준화의 정도(levels of standardisation), 구조생산공법(structural topology) 등의 변화에 따른 설계 결과의 민감도를 분석(sensitivity studies)하였다. 전산장비는 설계자의 대화형 접근을 용이하도록 하기 위해 VAX의 화상 처리장치를 이용하여 각 설계안에 대한 구조형상과 작업분석, 건조비 현황 등을 제시할 수 있도록 하였다. 결론적으로 본 연구는 설계초기 단계에서 상세한 건조비 모델(detailed production cost model)을 대화형 화상 처리방법에 접합시켜 이를 이용하여 여러가지 설계안의 도출과 비교검토를 신속히 처리할 수 있도록 함은 물론, 각종 생산 실적정보를 초기설계에 반영하는 최초의 시도라고 믿으며, 생산지향적(Design for Production) 최적설계분야의 발전에 많은 도움이 되기를 기대해 마지 않는다. 참고로 본 시스템의 설계 적용결과를 부록에 요약 소개하며, 상세한 내용은 참고문헌 [4] 또는 [7]을 참조 요망한다.

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Effects of modified atmosphere packaging (MAP) and vaporized ethyl pyruvate (EP) treatment for the shelf life of 'Seolhyang' strawberries (딸기 '설향' 품종의 MAP 및 ethyl pyruvate 처리의 유통기한 연장 효과 연구)

  • Kim, Jinse;Park, Jong Woo;Park, Seok Ho;Choi, Dong Soo;Kim, Yong Hoon;Lee, Soo Jang;Park, Chun Wan;Lee, Jung Soo;Cho, Byoung-Kwan
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.351-360
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    • 2017
  • We have studied the technology to extend the storage period of 'Seolhyang' strawberries using modified atmosphere package (MAP) and ethyl pyruvate (EP) treatment for domestic distribution and export. The selected ripe strawberries harvested on December 28, 2016 at the Sancheong farmhouse were transported to the laboratory for 2 h and tested. After a day's precooling at $4^{\circ}C$, the strawberries were divided into seven experimental groups. These groups were control, active MAP using low density polyethylene (LDPE), active MAP using polyamide (PA), active MAP using PA with EP treatment, passive MAP using LDPE, passive MAP using PA and passive MAP using PA with EP treatment. Quality analysis was carried out every 4 days during the storage period of 16 days. During the storage period of 16 days, MAP decreased from 3.5% to less than 1.1% in weight loss ratio compared with control, and decreased from 36% to less than 7% in fungal incidence. In the case of fungi in the EP treatment group, hyphae did not grow on the outside of the strawberry but grew to the inside. This tendency was similar to that in the low oxygen and high carbon dioxide environment of the MAP, the mycelium of the fungus did not grow outside of the strawberry. Fungi are the biggest problem in the distribution and export of strawberries, and these results suggest that MAP alone could inhibit mold and increase shelf life.

Effects of Rhizobium Inoculation on the changes of Ureide-N and Amide-N Concentration in Stem and Root exudate of Soybean Plant (대두근류균(大豆根瘤菌) 접종(接種)이 뿌리와 줄기 즙액중(汁液中) Amide-N 및 Ureide-N 농도(濃度)에 미치는 영향(影響))

  • Ko, Jae-Young;Suh, Jang-Sun;Lee, Sang-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.329-336
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    • 1989
  • A series of green house experiment was conducted to find but the effect of fertilizer application and inoculation of rhizobium on the changes of amide-N, ureide-N and $NH_4-N$ concentration in stem and root exudates of soybean plant growth. The results obtained were summarized as follows ; 1. Five strains of indigenous Rhizobium japonicum-nitrogen fixing activity($C_2H_2$-reducing activity) was more than 6.4 to 20.1 nmole/hr/tube-were identified from 37 soil samples in 22 areas of farmers field throughout country. 2. These identified 5 strains of rhizobium were obtained high nitrate reductase but low ammonium and nitrite oxidase activities. Among 5 strains of rhizobium the Rhizobium japonicum RjK-134 was applied for this green house experiment. 3. Dry matter yield was increased by the combination of inoculation of Rhizobium japonicum RjK-134 with no fertilizer and without nitrogen fertilizer application. However, dry matter yield was decreased with application of N and NPK with inoculation of rhizobium. 4. The concentrations of amide-N and ureide-N were increased in xylem sap than that of root exudate and higher concentration was obtained ar 30 days after planting than flowering stage (45 days after planting). 5. The combination of NPK application with inoculation of Rhrizobium japonicum RjK-134 enhanced the increase of amide-N and ureide-N concentration in xylem sap and root exudate. 6. High ammonium-N concentration in xylem sap and root exudate were obtained in combination with without-fertilizer under no inoculation of rhizobium and N and NPK application with inoculation of rhizobium.

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Numerical Analysis of the Grand Circulation Process of Mang-Bang Beach-Centered on the Shoreline Change from 2017. 4. 26 to 2018. 4. 20 (맹방해빈의 일 년에 걸친 대순환과정 수치해석 - 2017.4.26부터 2018.4.20까지의 해안선 변화를 중심으로)

  • Cho, Young Jin;Kim, In Ho;Cho, Yong Jun
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.31 no.3
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    • pp.101-114
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    • 2019
  • In this study, we carry out the numerical simulation to trace the yearly shoreline change of Mang-Bang beach, which is suffering from erosion problem. We obtain the basic equation (One Line Model for shoreline) for the numerical simulation by assuming that the amount of shoreline retreat or advance is balanced by the net influx of longshore and cross-shore sediment into the unit discretized shoreline segment. In doing so, the energy flux model for the longshore sediment transport rate is also evoked. For the case of cross sediment transport, the modified Bailard's model (1981) by Cho and Kim (2019) is utilized. At each time step of the numerical simulation, we adjust a closure depth according to pertinent wave conditions based on the Hallermeier's analytical model (1978) having its roots on the Shield's parameter. Numerical results show that from 2017.4.26 to 2017.10.15 during which swells are prevailing, a shoreline advances due to the sustained supply of cross-shore sediment. It is also shown that a shoreline temporarily retreats due to the erosion by the yearly highest waves sequentially occurring from mid-October to the end of October, and is followed by gradual recovery of shoreline as high waves subdue and swells prevail. It is worth mentioning that great yearly circulation of shoreline completes when a shoreline retreats due to the erosion by the higher waves occurring from mid-March to the end of March. The great yearly circulation of shoreline mentioned above can also be found in the measured locations of shoreline on 2017.4.5, 2017.9.7, 2017.11.7, 2018.3.14. However, numerically simulated amount of shoreline retreat or advance is more significant than the physically measured one, and it should be noted that these discrepancies become more substantial for the case of RUN II where a closure depth is sustained to be as in the most morphology models like the Genesis (Hanson and Kraus, 1989).

Growth Characteristics and Ingredient Analysis According to the Transplanting Date and Distance of Dendranthema (L.). (감국 정식시기와 거리에 따른 생육특성과 성분 분석)

  • Jung, J.H.;Park, N.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.1
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    • pp.65-77
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    • 2020
  • In order to industrialize of Dendranthema indicum (L.) DesMoul., which is a lot of commercially available and is synonymous with chrysanthemum tea, in the autumn of 2018, Dendranthema indicum (L.) DesMoul. seeds were collected from its own native region, and the seeds were germinated after refrigerated storage. Young seedlings were subjected to experiments in February, March, and April in the open field to examine the effects on the harvesting of leaves by distance and the growth of leaves and stems. The results of analyzing the components by collecting the leaves+stem after collecting the flower of Dendranthema indicum (L.) DesMoul. are as follows. 1. When D. indicum (L.) DesMoul. seedlings were planted according to the transplanting date, the number of flowers was 17.1 in the transplanting date in April. The diameter of the flower was 2.9cm, 16ea, 6.5~6.6g in the fresh weight, and the dry weight of the case was 1.1~1.2g. The leaves were 46~47ea in March and April in the planted area, 5.2~5.3cm in leaf length and 3.5~3.6cm in leaf width. 2. When planted D. indicum (L.) DesMoul. seedlings according to transplanting distance, the number of flowers was 16.2 when planted at 20×20cm intervals and, 16.8~17.1 at 30×30~50×50cm intervals. The diameter of the flower was 2.7~2.8cm, the number of petals was 8, the length of the petal was 0.8 cm, and fresh weight was 6.5~6.6g per flower. Leaves had the largest number of 47 of 30×30cm and 40×40cm, and leaf length appeared at the longest 6.2cm in the 50×50cm treatment area, but 5.2cm in the other treatment areas. 3. The extraction yield of D. indicum (L.) DesMoul. leaves+stems was 7.93%, and the extraction solvent colors were light green at 50, 60% and green at 70, 80, 90, 100%. The extraction yield of D. indicum (L.) DesMoul. flowers was 7.58%, the color of the extraction solvent was light yellow at 50, 60 and 70%, yellow at 80 and 90%, and dark yellow at 100%. 4. We confirmed 11 kinds of ingredients such as in D. indicum (L.) DesMoul. flowers are gallic acid, 4-hydroxy benzoic acid, methyl gallate, 4-hydroxy-3-methoxy benzoic, caffeic acid, salicylic acid, p-coumaric acid, sinapic acid, naringin, 4-melthoxyben, flavone. The content was 29.200-36.900ppm. 5. The components contained in the D. indicum (L.) DesMoul. leaf+stem, salicylic acid appeared at 6,129.526ppm, and the next 4-methoxyben was 1,966.714ppm. It was methyl gallate 8.197ppm, 4-hydroxy-3-methoxy benzoic 6.994ppm, caffeic acid 5.566ppm, flavone 4.522ppm, p-coumaric acid 3.787ppm, gallic acid 1.893ppm that appeared in the content below 10ppm.