• Title/Summary/Keyword: C center

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The Expression Analysis of Complement Component C3 during Early Developmental Stages in Olive Flounder (Paralichthys olivaceus)

  • Lee, Jang-Wook;Lee, Young Mee;Lee, Jeong-Ho;Noh, Jae Koo;Kim, Hyun Chul;Park, Choul-Ji;Park, Jong-Won;Hwang, In Joon;Kim, Sung Yeon
    • Development and Reproduction
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    • v.17 no.4
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    • pp.311-319
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    • 2013
  • Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival in such an environment. The key component of innate immune system, C3 is central protein of all activation pathways of the complement system, leading to inflammatory reactions, such as opsonisation, chemotaxis, and cell lysis of pathogens. Although, innate mechanisms is essential for survival in the early stage of development, little is known about defence mechanisms. In this study, the alignment analysis showed that amino acid sequence of C3 from olive flounder liver EST homologous to other known C3 sequences with 73-99% identity. Also, we examined the tissue distribution of olive flounder C3 and analyzed expression pattern from the fertilized egg until 28 days post hatching. As a result, olive flounder C3 mRNA was expressed only in the liver and the mRNA level more increased as developmental proceed during the early stage. These results may suggest that olive flounder C3 plays an important function in the early immune response of olive flounder larvae.

Selection and Analysis of Genomic Sequence-Derived RNA Motifs Binding to C5 Protein

  • Kim, Kwang-sun;Ryoo, Hye-jin;Lee, June-Hyung;Kim, Mee-hyun;Kim, Tae-yeon;Kim, Yool;Han, Kook;Lee, Seol-Hoon;Lee, Young-hoon
    • Bulletin of the Korean Chemical Society
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    • v.27 no.5
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    • pp.699-704
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    • 2006
  • Escherichia coli RNase P is a ribonucleoprotein composed of M1 RNA and C5 protein. Previously, analysis of RNA aptamers selected for C5 protein from a synthetic RNA library showed that C5 protein could bind various RNA molecules as an RNA binding protein. In this study, we searched cellular RNA motifs that could be recognized by C5 protein by a genomic SELEX approach. We found various C5 protein-binding RNA motifs derived from E. coli genomic sequences. Our results suggest that C5 protein interacts with various cellular RNA species in addition to M1 RNA.

The Effect of Slurry and Wafer Morphology on the SiC Wafer Surface Quality in CMP Process (CMP 공정에서 슬러리와 웨이퍼 형상이 SiC 웨이퍼 표면품질에 미치는 영향)

  • Park, Jong-Hwi;Yang, Woo-Sung;Jung, Jung-Young;Lee, Sang-Il;Park, Mi-Seon;Lee, Won-Jae;Kim, Jae-Yuk;Lee, Sang-Don;Kim, Ji-Hye
    • Journal of the Korean Ceramic Society
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    • v.48 no.4
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    • pp.312-315
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    • 2011
  • The effect of slurry composition and wafer flatness on a material removal rate (MRR) and resulting surface roughness which are evaluation parameters to determine the CMP characteristics of the on-axis 6H-SiC substrate were systematically investigated. 2-inch SiC wafers were fabricated from the ingot grown by a conventional physical vapor transport (PVT) method were used for this study. The SiC substrate after the CMP process using slurry added oxidizers into slurry consisted of KOH-based colloidal silica and nano-size diamond particle exhibited the significant MRR value and a fine surface without any surface damages. SiC wafers with high bow value after the CMP process exhibited large variation in surface roughness value compared to wafer with low bow value. The CMPprocessed SiC wafer having a low bow value of 1im was observed to result in the Root-mean-square height (RMS) value of 2.747 A and the mean height (Ra) value of 2.147 A.

A Fluorescence-based cDNA-AFLP Method for Identification of Differentially Expressed Genes

  • Park, Sook-Young;Jwa, Nam-Soo;Chi, Myoung-Hwan;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.25 no.2
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    • pp.184-188
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    • 2009
  • Identification of differently expressed genes under specific tissues and/or environments provides insights into the nature and underlying mechanisms of cellular processes. Although cDNA-AFLP (Amplified Fragment Length Polymorphism) is a powerful method for analyzing differentially expressed genes, its use has been limited to the requirement of radioactive isotope use and the difficulty of isolating the bands of interest from a gel. Here, we describe a modified method for cDNA-AFLP that uses a fluorescence dye for detection and isolation of bands directly from a small size polyacrylamide gel. This method involves three steps: (i) preparation of cDNA templates, (ii) PCR amplification and differential display, and (iii) identification of differentially expressed genes. To demonstrate its utility and efficiency, differentially expressed genes during vegetative growth and appressorial development of Magnaporthe oryzae were analyzed. This method could be applied to compare gene expression profiles in a diverse array of organisms.

Response of Germination Rate to Variable Drying Conditions and Moisture Contents for Storage of Dehisced Korean Ginseng Seeds

  • Lee, Young-yi;Rajametov, Sherzod;Kim, Young-chang;Yi, Jung-yoon;Lee, Gi-an;Gwak, Jae-gyun;Son, Eun-ho;Park, Hong-jae
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.670-678
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    • 2016
  • We compared the germination rate of dehisced ginseng (Panax ginseng) seeds that were dried under two different conditions, slowly at $15^{\circ}C$ [relative humidity (RH) 10-12%] and rapidly under a laminar airflow cabinet at $25^{\circ}C$ (RH 22-25%). The measurements showed that drying rate and seed moisture content (SMC) play important roles in storage ability and vigor. The seeds that were dried rapidly at $25^{\circ}C$ showed high GR compared with the seeds that were dried at $15^{\circ}C$ after 6 and 12 months of storage at $-80^{\circ}C$ irrespective of MC. Seeds dried slowly at $15^{\circ}C$ with MC higher than 7.0% showed high GR maintenance after storage at $-18^{\circ}C$ and at $4^{\circ}C$ in comparison with rapidly dried seeds. However, the GR of the slowly desiccated seeds decreased as mean SMC was reduced to less than 5.0%, whereas the rapidly dried seeds were distinguished by significantly high GR irrespective of the storage conditions. The ginseng seeds desiccated under different conditions showed differences in storage performance. Seeds with 7-9% MC that were dried slowly at $15^{\circ}C$ for 5-7 days showed high GR after $4^{\circ}C$ and $-18^{\circ}C$ storage; however, longer periods of desiccation decreased the germination level remarkably compared with that of rapidly dried seeds.

Performance of Mobile WiMAX Femtocell AP (Mobile WiMAX Femtocell 기지국의 성능 분석)

  • Hwang, Inyong;Kim, Hyungkee;Kim, Seokjoong
    • Proceedings of the Korea Information Processing Society Conference
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    • 2013.11a
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    • pp.230-232
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    • 2013
  • 본 논문에서는 실내 음영지역에서 사용되는 모바일 와이맥스의 펨토셀 기지국(WFAP; WiMAX Femtocell AP)에 대한 성능을 분석하였다. 시뮬레이션을 통해 와이맥스 펨토셀 기지국의 패킷에러율(PER), Outage, throughput을 확인하였다.

Development of the Printed Top Gate Organic Thin Film Transistor (OTFT)

  • Kang, H.S.;Kang, H.C.;Lee, M.H.;Park, S.Y.;Kim, M.J.;Heo, J.S.;Kim, D.W.;Noh, Y.H.;Lee, S.;Kim, J.Y.;Kim, C.D.;Kang, I.B.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.113-116
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    • 2008
  • The active layer thickness and curing condition dependent performance of an organic thin film transistor (OTFT) with inkjetted organic semiconductor (OSC) layer is studied The best performance of the OTFT was found when the thickness of ose was ~120 nm cured at $60^{\circ}C$. The performance enhancement of the OTFT with inkjetted OSC layer was discussed by comparing the OTFT with spin-coated ose layer.

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