Browse > Article
http://dx.doi.org/10.12717/DR.2013.17.4.311

The Expression Analysis of Complement Component C3 during Early Developmental Stages in Olive Flounder (Paralichthys olivaceus)  

Lee, Jang-Wook (Genetics and Breeding Research Center, NFRDI)
Lee, Young Mee (Genetics and Breeding Research Center, NFRDI)
Lee, Jeong-Ho (Genetics and Breeding Research Center, NFRDI)
Noh, Jae Koo (Genetics and Breeding Research Center, NFRDI)
Kim, Hyun Chul (Genetics and Breeding Research Center, NFRDI)
Park, Choul-Ji (Genetics and Breeding Research Center, NFRDI)
Park, Jong-Won (Genetics and Breeding Research Center, NFRDI)
Hwang, In Joon (Genetics and Breeding Research Center, NFRDI)
Kim, Sung Yeon (Genetics and Breeding Research Center, NFRDI)
Publication Information
Development and Reproduction / v.17, no.4, 2013 , pp. 311-319 More about this Journal
Abstract
Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival in such an environment. The key component of innate immune system, C3 is central protein of all activation pathways of the complement system, leading to inflammatory reactions, such as opsonisation, chemotaxis, and cell lysis of pathogens. Although, innate mechanisms is essential for survival in the early stage of development, little is known about defence mechanisms. In this study, the alignment analysis showed that amino acid sequence of C3 from olive flounder liver EST homologous to other known C3 sequences with 73-99% identity. Also, we examined the tissue distribution of olive flounder C3 and analyzed expression pattern from the fertilized egg until 28 days post hatching. As a result, olive flounder C3 mRNA was expressed only in the liver and the mRNA level more increased as developmental proceed during the early stage. These results may suggest that olive flounder C3 plays an important function in the early immune response of olive flounder larvae.
Keywords
Olive flounder (Paralichthys olivaceus); Complement; C3; Early developmental stage; Gene expression;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Sunyer JO, Tort L, Lambris JD (1997) Diversity of the third form of complement, C3, in fish: functional characterization of five forms of C3 in the diploid fish Sparus aurata. Biochem J 326:877-881.
2 Thompson JD (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuc Acids Res 22:4673-4680.   DOI   ScienceOn
3 Yazawa R, Hirono I, Aoki T (2005) Characterization of promoter activities of four different Japanese flounder promoters in transgenic zebrafish. Mar Biotechnol 21:625-633.
4 Zapata AG, Torroba M, Varas A, Jimenez E (1997) Immunity in fish larvae. Developments in Biological Standardizations 90:23-32.
5 Zarkadis IK, Sarrias MR, Sfyroera G, Sunyer JO, Lambris JD (2001) Cloning and structure of three rainbow trout C3 molecules: a plausible explanation for their functional diversity. Dev Comp Immunol 25:11-24.   DOI   ScienceOn
6 Zhu Y, Thangamani S, Ho B, Ding JL (2005) The ancient origin of the complement system. EMBO J 24:382-394.   DOI   ScienceOn
7 Lovoll M, Johnson H, Boshra H, Bogwald, Sunyer JO, Dalmo RA (2007) The ontogeny and extrahepatic expression of complement factor C3 in Atlantic salmon (Salmo salar). Fish Shellfish Immunol 23:542-552.   DOI   ScienceOn
8 Lovoll M, Kilvik T, Boshra H, Bogwald J, Sunyer JO, Dalmo RA (2006) Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf and Df to offspring in rainbow trout (Oncorhynchus mykiss). Immunogenet 58:168-179.   DOI
9 Martin SA, Caplice NC, Davey GC, Powell R (2002) EST-based identification of genes expressed in the liver of adult Atlantic salmon (Salmo salar). Biochem Biophys Res 293:578-585.   DOI   ScienceOn
10 Matsushita M, Fujita T (1995) Cleavage of the third component of complement (C3) by mannose-binding protein associated serine protease (MASP) with subsequent complement activation. Immunobiology 194:443-448.   DOI   ScienceOn
11 Nakao M, Mutsuro J, Obo R, Fujiki K, Nonaka M, Yano T (2000) Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio): presence of variants lacking the catalytic histidine. Eur J Immunol 30:858-866.   DOI
12 Nakao M, Yano T (1998) Structural and functional identification of complement components of the bony fish, carp (Cyprinus carpio). Immunol Rev 166:27-38.   DOI   ScienceOn
13 Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener, S. & S.A. Krawetz (Eds). Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press Inc, Totowa (NJ), pp.365-386.
14 Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319-326.   DOI   ScienceOn
15 Parsons JD (1995) Improved tools for DNA comparison and clustering. Comput Appl Biosci 11:603-613.
16 Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45.   DOI   ScienceOn
17 Smith LC, Azumi K, Nonaka M (1999) Complement systems in invertebrates. The ancient alternative and lectin pathways. Immunopharmacol 42:107-120.   DOI   ScienceOn
18 Sunyer JO, Lambris JD (1998) Evolution and diversity of the complement system of poikilothermic vertebrates. Immunol Rev 166:39-57.   DOI   ScienceOn
19 Byon JY, Ohira T, Hirono I, Aoki T (2006) Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a microarray analysis. Vac 9: 921-930.
20 Dalmo RA (2005) Ontogeny of the fish immune system. Fish Shellfish Immunol 19:395-396.   DOI   ScienceOn
21 Del Rio-Tsonis K, Tsonis PA, Zarkadis IK, Tsagas AG, Lambris JD (1998) Expression of the third component of complement, C3, in regenerating limb blastema cells of urodeles. J Immunol 161:6819-6824.
22 Dumrongphol Y, Hirota T, Kondo H, Aoki T, Hirono I (2009) Identification of novel genes in Japanese flounder (Paralichthys olivaceus) head kidney up-regulated after vaccination with Streptococcus iniae formalin-killed cells. Fish Shellfish Immunol 26:197-200.   DOI   ScienceOn
23 Fearon DT, Locksley RM (1996) The instructive role of innate immunity in the acquired immune response. Science 272:50-53.   DOI   ScienceOn
24 Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150-163.   DOI   ScienceOn
25 Gongora R, Figueroa F, Klein J (1998) Independent duplications of Bf and C3 complement genes in the zebrafish. Scand J Immunol 48:651-658.   DOI   ScienceOn
26 Holland MCH, Lambris JD (2002) The complement system in teleosts. Fish Shellfish Immunol 12:399-420.   DOI   ScienceOn
27 Ikeda K, Sannoh T, Kawasaki N, Kawasaki T, Yamashita I (1987) Serum lectin with known structure activates complement through the classical pathway. J Biol Chem 262:7451-7454.
28 Lange S, Bambir S, Dodds WA, Magnadottir B (2004c) An immunohistochemical study on complement component C3 in juvenile Atlantic halibut (Hippoglossus hippoglossus L.). Dev Comp Immunol 28:593-601.   DOI   ScienceOn
29 Lange S, Bambir S, Dodds WA, Magnadottir B (2004b) The ontogeny of complement component C3 in Atlantic cod (Gadus morhua L.)-an immunohistochemical study. Fish Shellfish Immunol 16:359-367.   DOI   ScienceOn
30 Lange S, Dodds AW, Magnadottir B (2004a) Isolation and characterisation of complement component C3 from Atlantic cod (Gadus morhua L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Fish Shellfish Immunol 16:227-239.   DOI   ScienceOn
31 Boshra H, Li J, Sunyer JO (2006) Recent advances on the complement system of teleost fish. Fish Shellfish Immunol 20:239-262.   DOI   ScienceOn
32 Abelseth TK, Stensvag K, Espelid S, Nygaard R, Ellingsen T, Bogwald J, Dalmo RA (2003) The spotted wolffish (Anarhichas minor Olafsen) complement component C3: isolation, characterisation and tissue distribution. Fish Shellfish Immunol 15:13-27.   DOI   ScienceOn
33 Al-Sharif WZ, Sunyer JO, Lambris JD, Smith LC (1998) Sea urchin coelomocytes specifically express a homologue of the complement component C3. J Immunol 160: 2983-2997.
34 Aoki T, Nam B-H, Hirono I, Yamamoto E (1999) Sequences of 596 cDNA clones (565,977 bp) of Japanese flounder (Paralichthys olivaceus) leukocytes infected with Hirame Rhabdovius. Mar Biotechnol 1:477-488.   DOI   ScienceOn
35 Nonaka M (2004) Evolution of the complement system. Mol Immunol 40:897-902.   DOI   ScienceOn