• 생태와환경
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• 제37권1호통권106호
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• pp.122-129
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• 2004

In an effort to develop the biomarker for monitoring the contamination of xenoestrogen in the freshwater environment of Korea, reverse transcription-polymerasechain reaction (RT-PCR) analysis of vitellogenin (VTG) gene expression was optimized in Hearisarsus Iaseo, Based on the homology of the VTG cDNA sequences between the common carp and zebra fish, a set of PCR primers for VTG mRNA amplification for H; labo was designed. VTG mRNA level in livers from female and male fishes was analyzed by RT-PCR following single injection of 17 beta estradiol($E_2$ 10 mg $kg^{-1}$ B.W.). As an internal control, beta actin mRNA was amplified. One us of total liver RNA was subjected to RT-PCR. In female the amount of PCR productof VfC gradually increased in the range from 16 to 34 cycles of amplification. On the contrary, in control male, PCR product first detected at 32 cycles of amplification and linearly increased up to 40 cycles of amplification. In $E_2$ injected male liver, the VTC mRNA level was similar to that in the female. Taken together, this result suggests that liver of male H. labo expresses minute amount of VTG mRNA which are2-l6 equivalent of female and that induction of VTG mRNA occurs in male liver after estrogen treatment. In conclusion, the optimized protocol for RT-PCR analysis of VTG mRNA expression in liver of male H. labo will provide the environmental monitoring method for the xenoestrogen contamination in the rivers in Korea.

• Composites Research
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• 제17권6호
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• pp.52-57
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• 2004

본 연구에서는 환경 챔버를 이용한 극저온 환경에서, 열.하중 사이클에 따른 탄소섬유강화 복합재의 인장 물성 변화를 고찰하였다. Graphite/epoxy 일방향 복합재 시편에 대하여 시편 상온파손하중의 절반을 가한 상태에서, 상온에서 $-50^{\circ}C$, $-100^{\circ}C$, 그리고 $-150^{\circ}C$ 까지 각각 3회, 6회, 그리고 10회의 열-하중 사이클을 수행한 후 복합재의 인장 강도와 강성을 측정하였다. 그 결과, 온도가 낮아질수록 복합재의 인장 강성은 증가한 반면, 인장 강도는 감소함을 보였다. 그러나 복합재의 인장 강성은 저온 사이클 횟수에 거의 영향을 받지 않았으며 인장 강도는 사이클을 수행하지 않았을 때 보다 오히려 저온 사이클 수행 후 증가함을 확인할 수 있었다. 따라서 실험결과의 고찰을 위해 저온에서 복합재 시편의 열팽창계수를 측정하였고, 주사 전자 현미경 사진을 통해 섬유와 모재의 계면을 분석하였다.

• Applied Microscopy
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• 제33권1호
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• pp.49-58
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• 2003

EF-TEM 직접가열 실험을 통하여 titanium의 ${\alpha}-{\beta}$상전이를 연구하였다. 통계적 오차를 줄이기 위해 서로 다른 3군데의 titanium foil의 영역을 관찰하였고, 각각의 영역에 대해 단계별로($RT{\rightarrow}600{\rightarrow}900{\rightarrow}RT$) 회절패턴과 이미지를 기록하였다. 이 연구를 통해 얻은 결과는 다음과 같다. (1) Titanium은 $900^{\circ}C$에서 급격히 상전이가 진행된다. 이 온도에서는 ${\alpha}$와 ${\beta}$-상이 같이 존재한다. (2) 상전이가 일어난 ${\beta}$-상의 영역은 쌍정구조를 가진 plate 형태로 나타나며, 그들은 서로 상호 회전 배열되어 있다. (3) 전자회절도형과 EDS 분석 결과, $600^{\circ}C$ 이상의 가열에서는 열적 산화에 의해 Ti의 산화물이 표면에서 생성되기 시작하며 이들은 냉각 시 Ti의 ${\beta}{\rightarrow}{\alpha}$ 가역 상전이를 저해한다.

• Journal of Welding and Joining
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• 제28권5호
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• pp.45-50
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• 2010

The fatigue crack growth characteristics of base metal and weld joint of 9% Ni steel for LNG storage tank was carried out using CT specimen at room temperature and $-162^{\circ}C$. Fatigue crack growth rate of base and weld metals at RT and $-162^{\circ}C$ was coincided with a single line independent of the change of stress ratio and temperature. In the region of lower stress intensity factor range, fatigue crack growth rate at $-162^{\circ}C$ was slower than that at RT, and the slop of fatigue crack growth rate at $-162^{\circ}C$ increased sharply with propagating of fatigue crack, fatigue crack growth rate at RT and $-162^{\circ}C$ was intersected near the region of $2{\times}10-4\;mm$/cycle, and after the intersection region, fatigue crack growth rate at $-162^{\circ}C$ was faster than that at RT. The micro-fracture mechanism using SEM shows the ductile striation in the stable crack growth region. Also the defects of weld specimen after fatigue testing were detected using the A scan of ultrasonic apparatus.

• 한국식품과학회지
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• 제12권4호
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• pp.285-291
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• 1980

아끼바레 및 밀양 23호의 수화 및 취반속도를 비교하였다. 수화속도는 $10{\sim}40^{\circ}C$ 범위에서 무게증가로서 측정한 결과, 수화시간의 평방근의 비례하였다. 확산 계수는 Arrhenius 방정식으로 표시되었다. 아끼바레의 확산계수는 $3.151{\times}10^{-3}\exp(-4000/RT)$, 밀양 23호는 $5.853{\times}10^{-3}\exp(-5700/RT)$로 표시될 수 있었다. 취반속도는 밀양 23호가 아끼바레보다 빨랐으며 취반활성화에너지는 $90{\sim}100^{\circ}C$에서 밀양 23호가 아끼바레보다 다소 높았다. 밥의 조직특성은 밀양 23호의 부착성이 아끼바레보다 높았고, 아밀로 그라프의 점도도 밀양 23호가 높았다.

• Asian-Australasian Journal of Animal Sciences
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• 제6권2호
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• pp.275-279
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• 1993

A study using dairy heifers was conducted to determine the effect of environmental temperature on heat production differing in feed intake level. The design consisted of three levels of feed intake (low, medium and high) and two environmental chamber temperature (15 and $30^{\circ}C$) with four replications in each treatment. Rectal temperature (RT), respiration rate (RR), heart rate (HR) and heat production (HP) were then measured. At the both environmental temperature, RT, RR and HR increased with the increase in feed intake level. The RT and RR also increased with the elevation of environmental temperature. The HP of $30^{\circ}C$ was significantly higher (4.8-8.9%) than $15^{\circ}C$. The estimated metabolizable energy requirement for maintenance (MEm) was higher (p<0.05) at $30^{\circ}C$ ($554.7kJ/kg^{0.75}$ d) than $15^{\circ}C$ (464.9 kJ/kgd). It was suggested that the decreasing in productive efficiency under hot environmental conditions partly associated with the increase in HP, which associated with the change in heat loss mechanism from sensible path to evaporative path.

• 대한통합의학회지
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• 제7권1호
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• pp.65-74
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• 2019

Purpose : The purpose of this study was to determine the effects of 12 weeks of circuit training on inflammatory markers, blood lipids, and body composition of obese middle-aged women. Methods : Twenty-four obese women were randomly divided into three groups (circuit exercise training, CT; aerobic exercise training, AT; resistance exercise training, RT). All the subjects participated in the exercise program for 60 minutes, 3 times a week for 12 weeks. Results : Significant differences found in the participants pre-and post exercise training C-reactive protein (CRP), fibrinogen, and adiponectin levels. The CT participants experience an expected increase in their adiponectin levels. Significantly improved blood TC, LDL-C, HDL-C, and TG levels were found after 12 weeks of exercise. The AT and CT groups showed greater improvements in their HDL-C, LDL-C, and TG levels than the RT group. Significant differences were found in the participants pre-and post-exercise training body weight, body fat percentage, and body mass index (BMI). Conclusion : The CT participants body fat percentages decreased more than the RT group's percentages. In conclusion, obese women who completed 12 weeks of circuit exercise training (aerobics + resistance exercise) had significant improvements in their inflammatory markers, blood lipids, and body fat percentage.

• Journal of Chest Surgery
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• 제13권2호
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• pp.130-133
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• 1980

Since the first report of an operation for prolapsed intervertebral disk by Mixter and Barr[1934], many thousands of operations have been successfully performed without incident. Linton and White in 1945 reported the vascular complication, but perforation of large vessels is rare complication of operation for prolapsed disk. A medical student, aged 22 years, was performed to a disk operation [L4-5, Rt. on May 1977.] From postoperative 10th day, palpitation, generalized edema and substernal pain were noted, and 2 months later, wide pulse pressure [70-80 mmHg], continuous bruit and thrill on the Rt. low abdomen were followed. Aortography revealed arteriovenous fistula between just proximal to abdominal aortic bifurcation and inferior vena cava. So, fistulectomy [Resection of proximal 2 cm of C.I.A., Rt, including fistula opening and end to end anastomosis] was performed on July, 77. During follow up study, remained fistula between Rt. internal lilac artery and lilac vein was found 2 months later. Re-operation [Double ligation of the Rt. internal lilac artery] was don on January 1978. Postop. results were excellent, except impossible to ejaculation.

• The Plant Pathology Journal
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• 제16권1호
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• pp.48-51
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• 2000

Citrus tristeza virus (CTV) was identified form CTV-infected early satsuma mandarin (Citus unshiu) and yuzu (C.junos) by RT-PCR. The total RNAs were isolated from citrus bark and seaf tissues infected with CTV and reverse transcription was followed with primers designed for amplifying CTV coat protein gene. DNA fragments 738 bp were amplified by RT-PCR and these products were colned for sequence analysis. Based on the sequence analysis, this PCR product has 97% sequence homology to CTV (T-385) CP gene isolated from USA. RT-PCR assay for CTV detection was more sensitivity than ELISA assay which was done with anti-CTV CP antibody. This is the frist report about CTV identification in Cheju island Korea.

• 한국식물병리학회지
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• 제12권3호
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• pp.358-362
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• 1996

감자 잎말림 바이러스를 검정하기 위하여 ELISA 및 전자현미경에 의해 바이러스 감염이 확인된 기내 배양중인 감장의 줄기로부터 RT-PCR 분석을 수행하였다. 분리된 총 RNA들로부터 바이러스 cDNA를 합성하고 감자 잎말림 바이러스 외피단백질의 일부인 465bp를 특이하게 증폭하도록 고안한 두 primer를 사용하여 PCR 반응을 하였다. 증폭된 465pb의 DNA 절편의 염기서열을 분석한 결과 역시 감자 잎말림 바이러스임을 확인하였다. 바이러스 검정에 있어서 EL-ISA 방법과 RT-PCR 방법간의 민감도를 조사한 결과 RT-PCR 방법간의 민감도를 조사한 결과 RT-PCR 방법이 ELISA 방법보다 감자 잎말림 바이러스검정에 있어서보다 정확한 방법인 것으로 사료된다.