• The Korean Journal of Physiology and Pharmacology
• /
• v.2 no.3
• /
• pp.377-384
• /
• 1998

Gold compounds depress phagocytic cell responses, including chemotaxis, and respiratory burst. However, the effects of gold compounds on the function of phagocytic cells are variable according to the preparation of medicine. In this study, effect of tetrachloroauric acid on activated neutrophil responses, including respiratory burst, lysosomal enzyme release and change of intracellular $Ca^{2+}$ level and on the synthesis of interleukin-8 and granulocyte-macrophage colony stimulating factor by macrophages was studied. This study further examines how gold compounds affect the activation processes. The respiratory burst stimulated by complement C5a, degraded IgG and PMA in neutrophils was inhibited by tetrachloroauric acid. In contrast to C5a and degraded IgG, PMA-stimulated superoxide production was weakly inhibited by tetrachloroauric acid. Staurosporine, genistein, EGTA and verapamil inhibited superoxide and $H_2O_2$ production caused by C5a and degraded IgG. PMA-stimulated superoxide production was inhibited by staurosporine but was not affected by genistein. Tetrachloroauric acid, genistein, EGTA and verapamil inhibited the release of acid phosphatase and myeloperoxidase, while the effect of staurosporine was not detected. The synthesis of interleukin-8 and granulocyte-macrophage colony stimulating factor by $interleukin-1{\beta}$ in macrophages was inhibited by tetrachloroauric acid. Preincubation with tetrachloroauric acid, genistein, EGTA and verapamil, the elevation of [$Ca^{2+}_i$] evoked by C5a was inhibited. Store-regulated $Ca^{2+}$ entry in thapsigargin-pretreated neutrophils was decreased by the addition of tetrachloroauric acid and genistein. The effect of staurosporine on intracellular $Ca^{2+}$ mobilization was not observed. In conclusion, tetrachloroauric acid may suppress neutrophil responses through its inhibitory action on elevation of intracellular $Ca^{2+}$ level and protein kinase C. It might exhibit an inhibitory effect on the action of protein tyrosine kinase. Tetrachloroauric acid depresses cytokine production by macrophages.

• Biomaterials and Biomechanics in Bioengineering
• /
• v.1 no.1
• /
• pp.13-25
• /
• 2014

In the current study, a drug-eluting stent coated with biodegradable polymers and sirolimus was developed by using an ultrasonic nanocoater and characterized in aspects of surface smoothness and coating thickness. In addition, in vitro release profiles of sirolimus by changing top coating layer with different biodegradable polymers were investigated. Smooth surfaces with variable thickness could be fabricated by optimizing polymer concentration, flow rate, nozzle-tip distance, gas pressure, various solvents and ultrasonic power. Smooth surface could be generated by using volatile solvents (acetone, chloroform, and methylene chloride) or post-treating with solvent vapor. Coating thickness could be controlled by varying injection volume or polymer concentration, and higher concentration could reduce the coating time while obtaining the same thickness. The thickness measurement was the most effectively performed by a conventional cutting method among three different methods that were investigated in this study. Release profiles of sirolimus were effectively controlled by changing polymers for top layer. PLGA made the release rate 3 times faster than PDLLA and PLLA and all top layers prevented burst release at the initial phase of profiles. Our results will provide useful and informative knowledge for developing drug-eluting stents, especially coated with biodegradable polymers.

• Journal of Pharmaceutical Investigation
• /
• v.27 no.1
• /
• pp.71-77
• /
• 1997

With the aim of improving periodontal regeneration efficacy, as a biodegradable local drug delivery device, drug releasing chitosan beads were prepared. Chitosan beads were prepared through the formation of intermolecular or intramolecular ionic interaction bewteen chitosan and sodium tripolyphosphate and were loaded with flurbiprofen. The mean diameter of the beads was $250\;{\mu}m$. Drug loading efficiency was improved by regulating the pH of tripolyphosphate solution. The drug release kinetics mainly depended upon the hydrophobic properties of the flurbiprofen, that is, the release of flurbiprofen showed initial burst with rapid release for the first day followed by a levelling off of the release rate. However, the release rate could be controlled by the formulation factor including the pH, concentration of the tripolyphosphate solution, gelation time, drug contents. From these results, flurbiprofen loaded chitosan beads were anticipated as biodegradable local drug delivery devices for periodontal regeneneration.

• MALSORI
• /
• no.53
• /
• pp.1-16
• /
• 2005

This experimental study aims to test the hypothesis that the occurrence of English sentence-final stop release is, at least, partly predictable by examining its phonetic context. 10 native(5 male and 5 female) speakers of American English recorded, in a sound-proof booth, sentences excerpted from novels and the natural documents on the World Wide Web. Based on the waveforms and spectrograms of the recorded sentences, judgements of the release of a sentence-final stop were made. If the aperiodic energy of a given final stop lasted more than .015 second, it was considered to be "released." The result reveals that English sentence-final stops tend to be released when they are 1) velar consonants, 2) preceeded by tense vowels, and 3) coda consonants of content words. The phonetic environment in which final stops are often released can be characterized by the articulatory comfortableness and the need for release burst noise, without which the final stops may not be correctly perceived. By examining the release of English final stops, it is concluded that the phonological events, which had been considered to occur rather "randomly," in fact, reflect the universal tendency of human speech: to minimize the speakers' and hearers' effort.

• Macromolecular Research
• /
• v.13 no.5
• /
• pp.397-402
• /
• 2005

To explore the potential of shell crosslinked micelle (SCM) as a drug carrier, the drug release behavior of poly($\varepsilon$-caprolactone)-b-poly(acrylic acid) (PCL-b-PAA) SCMs was investigated. PCL-b-PAA was synthesized by ring opening polymerization of $\varepsilon$-caprolactone and atom transfer radical polymerization of tert-butyl acrylate, followed by selective hydrolysis of tert-butyl ester groups to acrylic acid groups. The resulting amphiphilic polymer was used to prepare SCMs by crosslinking of PAA corona via amidation chemistry. The drug release behavior of the SCMs was studied, using pyrene as a model drug, and was compared with that of non-crosslinked micelles, especially below the critical micelle concentration (CMC). When the shell layers were crosslinked, the drug release behavior of the SCMs was successfully modulated at a controlled rate compared with that of the non-crosslinked micelles, which showed a burst release of drug within a short time.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.27 no.2
• /
• pp.312-316
• /
• 2013

Caffeine is a thermogenic agent that can be used in weight loss products. In order to achieve a sustained release of caffeine, silk fibroin (SF) film was uses as carrier. It has been shown that the loading method of caffeine into SF film affected the uniform distribution of caffeine in the SF film. When caffeine was added directly into SF solution, gelation has been occurred immediately and prevented the uniform distribution of caffeine. On the other hand, caffeine was dissolved in methanol in order to load the caffeine in SF film and crystallize the SF film at the same time. However, due to the fast evaporation of methanol, caffeine was recrystallized on the surface of SF film rather than penetrating into the film. Finally, caffeine was loaded into pre-crystallized SF film and uniform distribution of caffeine could be achieved. There was an initial burst of caffeine during the first 15 min, but after that a sustained release was achieved.

• YAKHAK HOEJI
• /
• v.47 no.2
• /
• pp.78-84
• /
• 2003

Local anesthetics are used to reduce pain, but they are so frequently injected to patients. So, we prepared lidocaine solid lipid microspheres (SLM) as long acting abdominal injection using spray drying method and evaluated drug entrapment, particle size, SEM, zeta potential and in vitro and in vivo drug release pattern, The particle sizes of SLM were 30∼100$\mu$m and it is enough to inject into abdominal tissue. The entrapment efficiency of SLM was over 95％ as spray drying method. Surfactant and PC decreased the burst effect by 20∼30％. In in vivo test, C-6 showed controlled release concentration profile in plasma for 8 days and C-5 sustained longer than we expected.

• Advances in materials Research
• /
• v.9 no.2
• /
• pp.109-114
• /
• 2020

The purpose of this study was to observe the trend of compounds release from acrylic resin oral prosthesis when used for drug delivery as well as a restoration. In this study, 10 specimens of heat-cured polymethylmethacrylate material were prepared and loaded with methylene blue biological stain. The specimens were then submerged in vials with 5 ml distilled water for 24 hours. The extraction procedure continued for 4 days, each day the specimens were immersed in another 5 ml distilled water vial. All extracted solutions were analyzed by visible light spectroscopy for absorbance comparison. The statistical results showed that the absorbance values were significantly different in the first day of extraction than the following days. However, there was no statistical difference among the 2^nd, 3^rd and 4^th days of extraction. Biological stain loading to acrylic resin at the mixing stage, and then after extraction in distilled water, showed a burst release during the first day followed by a constant release during the following few days.

• Composites Research
• /
• v.34 no.4
• /
• pp.257-263
• /
• 2021

Composite materials have been widely applied for fabricating pressure vessels used for storing gaseous and liquid fuel because of their high specific stiffness and specific strength. Accordingly, the accurate measurement of their mechanical property, particularly the burst pressure or fracture strain, is essential prior to the commercial release. However, verification of the safety of composite pressure vessels using conventional test methods poses some limitations because it may lead to the deformation of the load transferring media or provoke an additional energy loss that cannot be ignored. Therefore, in this study, the segment-type ring burst test device was designed considering the theoretical load transferring ratio and applicable displacement of the vertical column. Moreover, to verifying the uniform distribution of pressure of the segment type ring burst test device, the hoop stress and strain distribution of ring specimens were compared with that of the hydraulic pressure test method via FEM. To conduct a simulation of the fracture behavior of the composite pressure vessel, a Hashin failure criterion was applied to the ring specimen. Furthermore, the fracture strain was also measured from the experiment and compared with that of the result from the FEM.

• Korean Chemical Engineering Research
• /
• v.46 no.2
• /
• pp.205-210
• /
• 2008

Poly(D,L-lactide-co-glycolide) (PLGA) has been widely used as carriers in controlled release delivery systems due to its biodegradability and relatively good biocompatibility. However, Release pattern of carriers fabricated using PLGA have disadvantage an initial burst within a few days, lag time several days and then sudden release changes. To solve these problems of PLGA, we fabricated PLGA wafer including monomer. Also, drug release behavior restraint sudden burst effect using recrystallization of PLGA. Recrystallized PLGA was characterized the morphological difference by SEM and in vitro release behavior measured by HPLC. The PLGA molecular weight analyzed to recognize monomer influence during degradation process of polymer using GPC. In this study, drug release duration cut short up to three days and was eliminated the lag time based on the bulk erosion.