• Journal of Food Hygiene and Safety
• /
• v.5 no.3
• /
• pp.159-164
• /
• 1990

To detect and quantitation residual antibiotics and antibacterial agents in meats, we performed a biological assay employing the three microorganisms Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778 for the screening purpose and developed a Gas Chromatography-mass Spectrometry(GC/MS) analysis for the confirmation and quantiation. In the biological assay (paper disk method), three test solution are used depending on the character of the residual antibiotics and antibacterial agents, follow by a simple clean up procedure which includes homogenization with Mcilvaine buffer, defatting with includes homogenization with Mcilvaine buffer, defatting with hexane, extraction with chloroform, clean-up by Sep-Pak $C_{18}$ and Bakerbond SPE carboxylic acid column. The chloroform layer is used for the analysis of sulfa agents. macrolides antibiotics and antibacterial agents, Adsorbed materials in the Sep-Pak $C_{18}$ were also employed for th analysis of penicillins and tetracyclines. Effluents from the Sep-Pak $C_{18}$ were cleaned-up one more by Bakerbond 10 SPE COOH column and employed for the analysis of aminoglycosides. In the instrumental analysis by using the GC/MSD, residual antibiotics and antibacterial agent were quantitated by selected ion monitoring (SIM) mode after derivatization. A simultaneous analysis of six residual antibiotic and antibacterial agent such as oxytetracycline, penicillin, ampicillin, choliraphenicol and thiamphenicol was developed with simple cleanup procedures revealing good recovery and reproducibility. Also, simultaneous detection of macrolides antibiotics such as erythromycin, spiramycin, and oleandomycin was developed after acid hydrolysis due to their large molecular structures. Because of the high reproducibility and selectivity of these two methods, it is very desirable that the combination of the two methods be used in the bioassay for the screening of residual antibiotics and antibacterial agent and that GC/MSD analysis be used for the confirmation and quantitation.

• The Korean Journal of Zoology
• /
• v.33 no.4
• /
• pp.468-475
• /
• 1990

An In vitro protein sulfation in the soluble fraction of rat brain was charaderized further by an improved method of alkaline hydrolysis and thin layer ceflulose electrophoresis TLE) The protein sulfation was carried out in a reaction system containing [35 S] 3'-phosphoadenosine-5'-phosphosulfate (PAPS), Tris-maleate buffer (pH 8), MgCI$_2$, and soluble proteins from rat brain. The sulfated proteins were precipitated by acetone and alkaline hydrolysis was performed to obtain sulfated amino acids. The hydrolysate was separated further by TLE and the separated residues were identified by fluorography. The Iluorography of one-dimensional The showed at least nine sulfated residues including tryosine-O-sulfate. The other spots were not identified yet positively. General properties of protein sulfotransferases (PST) using this method were re-examined such as effects of concentrations of PAPS, pH, incubation temperature and $Mg^2$+. These results suggest a possible occurrence of several PST corresponding to each sulfated residue in rat brain and that the sulfation can occur not only in tyrosine but also in other residues as well.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.6A
• /
• pp.651-659
• /
• 2004

The Fast Handover protocol ［1］ provides seameless handover in wireless If networks by minimizing handover latency, which uses anticipation based on layer 2 (L2) trigger information. Therefore, it incurs higher signaling costs compared with the basic Mobile U protocol. Furthermore, since the L2 trigger is based on fluctuating wireless channel states, the handover anticipation may sometimes be incorrect. In the case of incorrect anticipation, unnecessary buffer space may be used for the purpose of providing a smooth handover. Therefore, it is essentical to analyze these overhead costs, in order to evaluate and compare the performance of Fast Handover with that of the basic Mobile U protocol. In this paper, we analyzed the overhead associated with Fast Handover including the signaling cost and the packet delivery cost. We formulated these costs based on a timing diagram and compared Fast Handover with basic Mobile Ipv6 in terms of their packet loss rates and buffer requirements. Also, we studied the impact of the L2 triggering time on the total overhead cost.

• Journal of Institute of Control, Robotics and Systems
• /
• v.19 no.7
• /
• pp.653-660
• /
• 2013

This paper deals with the problem of unfairness among uplink TCP (Transmission Control Protocol) flows associated with frame aggregation employed in IEEE 802.11n WLANs (Wireless Local Area Networks). When multiple stations have uplink TCP flows and transmit TCP data packets to an AP (Access Point), the AP has to compete for channel access with stations for the transmission of TCP ACK (acknowledgement) packets to the stations. Due to this contention-based channel access, TCP ACKs tend to be accumulated in the AP's downlink buffer. We show that the frame aggregation in the MAC (Medium Access Control) layer increases TCP ACK losses in the AP and leads to the serious unfair operation of TCP congestion control. To resolve this problem, we propose the TAC (TCP ACK Compression) mechanism operating at the top of the AP's interface queue. By exploiting the properties of cumulative TCP ACK and frame aggregation, TAC serves only the representative TCP ACK without serving redundant TCP ACKs. Therefore, TAC reduces queue occupancy and prevents ACK losses due to buffer overflow, which significantly contributes to fairness among uplink TCP flows. Also, TAC enhances the channel efficiency by not transmitting unnecessary TCP ACKs. The simulation results show that TAC tightly assures fairness under various network conditions while increasing the aggregate throughput, compared to the existing schemes.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.8 no.2
• /
• pp.307-314
• /
• 1998

PZT thin films were fabricated by the Sol-gel method. Starting materials used for the preparation of the stock solution were Pb-acetate trihydrate, Zr-normal propoxide and Ti-isopropoxide. 2-Methoxyethanol and iso-propanol were used for solution. For studying the diffusion of Pb ion into the substrates. We used bare Si substrate, $SiO_2/Si$ substrates which was produced by thermal oxidation and $TiO_2/SiO_2/Si$ which was mad by Sol-gel method. Densification and adhesion of thin films were observed by SEM. Phase formation of thin films and diffusion of Pb ion into the substrate were examined by XRD and ESCA, respectively. In the case of bare Si and $SiO_2/Si$ substrate, we obtained the perovskite phase at $700^{\circ}C$ and restricted a little the diffusion of Si ion into the film with $SiO_2$ buffer layer. In the case of $TiO_2/SiO_2/Si$, perovskite phase were obtained at $500^{\circ}C$ and the diffusion of Pb ion and Si ion were restriced.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.18 no.2
• /
• pp.87-90
• /
• 2008

Co-Pt alloy thin films were galvanostatically electrodeposited on Ru (30 nm)/Ta (5 nm)/Si (100) substrates from a amino-citrate based electrolyte. We used Ru(0002)-oriented buffer layers to control the crystallinity and orientation of the Co-Pt alloy thin films. The effect of solution temperature on the microstructure and magnetic properties of the Co-Pt alloy thin film was investigated. The samples were characterized by EDS, FESEM, XRD diffractometer using Cu $K{\alpha}$ radiation. The magnetic properties of these films were analyzed by a VSM and torque magnetometer. The Co-Pt alloy thin films were exhibited very high out-of-plane coercivity and squareness of the multilayer were 6527 Oe and 0.93, respectively, without heat treatment.

• The Transactions of the Korea Information Processing Society
• /
• v.7 no.8
• /
• pp.2543-2554
• /
• 2000

Packet schedulers for real-time communication must provide bounded delay and efficient use of network resources such as bandwidth, buffers and so on. In order to satisfy them, a large number of packet scheduling methods have been proposed. Among packet scheduling methods, an EDF (Earliest Deadline First) scheduling is the optimal one for a bounded delay service. A disadvantage of EDF scheduling is that queued packets must be sorted according to their deadlines, requiring a search operation whenever a new packet arrives at the scheduler. Although an RPQ (Rotating Priority Queue) scheduler, requiring large size of buffers, does not use such operation, it can closely approximate the schedulability of an EDF scheduler. To overcome the buffer size problem of an RPQ scheduler, this paper proposes a new scheduler named MRPQ (Multiple Rotating Priority Queue). In a MRPQ scheduler, there are several layers with a set of Queues. In a layer, Queues are configured by using a new strategy named block Queue. A MRPQ scheduler needs nearly half of buffer size required in an RPQ scheduler and produces schedulability as good as an RPQ scheduler.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2010.08a
• /
• pp.259-259
• /
• 2010

Periodically polarity inverted (PPI) ZnO structures on (0001) Al2O3 substrates are demonstrated by plasmas assisted molecular beam epitaxy. The patterning and re-growth methods are used to realize the PPI ZnO by employing the polarity controlling method. For the in-situ polarity controlling of ZnO films, Cr-compound buffer layers are used.[1, 2] The region with the CrN intermediate layer and the region with the Cr2O3 and Al2O3 substrate were used to grow the Zn- and O-polar ZnO films, respectively. The growth behaviors with anisotropic properties of PPI ZnO heterostructures are investigated. The periodical polarity inversion is evaluated by contrast images of piezo-response microscopy. Structural and optical interface properties of PPI ZnO are investigated by the transmission electron microcopy (TEM) and micro photoluminescence ($\mu$-PL). The inversion domain boundaries (IDBs) between the Zn and the O-polar ZnO regions were clearly observed by TEM. Moreover, the investigation of spatially resolved local photoluminescence characteristics of PPI ZnO revealed stronger excitonic emission at the interfacial region with the IDBs compared to the Zn-polar or the O-polar ZnO region. The possible mechanisms will be discussed with the consideration of the atomic configuration, carrier life time, and geometrical effects. The successful realization of PPI structures with nanometer scale period indicates the possibility for the application to the photonic band-gap structures or waveguide fabrication. The details of application and results will be discussed.

• Progress in Superconductivity
• /
• v.8 no.2
• /
• pp.175-180
• /
• 2007

High temperature superconductor $YBa_2Cu_3O_{7-x}$ (YBCO) films have been grown by in-situ electron beam evaporation on artificial metal tapes such as ion-beam assisted deposition (IBAD) and rolling assisted biaxially textured substrates (RABiTS). Deposition rate of the YBCO films is $10{\sim}100{\AA}/sec$. X-ray diffraction shows that the films are grown epitaxially but have inter-diffusion phases, like as $BaZrO_3\;or\;BaCeO_3$, at their interfaces between YBCO and yttrium-stabilized zirconia (YSZ) or $CeO_2$, respectively. Secondary ion mass spectroscopy depth profile of the films confirms diffused region between YBCO and the buffer layers, indicating that the growth temperature ($850{\sim}900^{\circ}C$) is high enough to cause diffusion of Zr and Ba. The films on both the substrates show four-fold symmetry of in-plane alignment but their width in the -scan is around $12{\sim}15^{\circ}$. Transmission electron microscopy shows an interesting interface layer of epitaxial CuO between YBCO and YSZ, of which growth origin may be related to liquid flukes of Ba-Cu-O. Resistivity vs temperature curves of the films on both substrates were measured. Resistivity at room temperature is between 300 and 500 cm, the extrapolated value of resistivity at 0 K is nearly zero, and superconducting transition temperature is $85{\sim}90K$. However, critical current density of the films is very low, ${\sim}10^3A/cm^2$. Cracking of the grains and high-growth-temperature induced reaction between YBCO and buffer layers are possible reasons for this low critical current density.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2010.08a
• /
• pp.232-232
• /
• 2010

Recently light emitting diodes (LEDs) have been expected as the new generation light sources because of their advantages such as small size, long lifetime and energy-saving. GaN, as a wide band gap material, is widely used as a material of LEDs and GaN nanorods are the one of the most widely investigated nanostructure which has advantages for the light extraction of LEDs and increasing the active area by making the cylindrical core-shell structure. Lately GaN nanorods are fabricated by various techniques, such as selective area growth, vapor-liquid-solid (VLS) technique. But these techniques have some disadvantages. Selective area growth technique is too complicated and expensive to grow the rods. And in the case of VLS technique, GaN nanorods are not vertically aligned well and the metal catalyst may act as the impurity. So we just tried to grow the GaN nanorods on Si substrate without catalyst to get the vertically well aligned nanorods without impurity. First we deposited the AlN buffer layer on Si substrate which shows more vertical growth mode than sapphire substrate. After the buffer growth, we flew trimethylgallium (TMGa) as the III group source and ammonia as the V group source. And during the GaN growth, we kept the ammonia flow stable and periodically changed the flow rate of TMGa to change the growth mode of the nanorods. Finally, as the optimization, we changed the various growth conditions such as the growth temperature, the working pressure, V/III ratio and the doping level. And we are still in the process to reduce the diameter of the nanorods and to extend the length of the nanorods simultaneously. In this study, we focused on the shape changing of GaN nanorods with different growth conditions. So we confirmed the shape of the nanorods by scanning electron microscope (SEM) and carried out the Photoluminescence (PL) measurement and x-ray diffraction (XRD) to examine the crystal quality difference between samples. Detailed results will be discussed.