• Journal of Sericultural and Entomological Science
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• v.15 no.2
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• pp.45-53
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• 1973

1. The sericin solubility increased rapidly as the increase of water M-alkalinity. 2. The acidity of the treated water was nutralized at the over 25ppm of M-alkalinity. 3. The more M-alkalinity of the sample water is, the more M-alkalinity was found after cocoon treat. 4. The total hardness of sample water seemed to be droped as M-alkalinity increased. 5. The sericin solubility also seemed to be droped as the increase of water acidity. 6. In case of treat finish with cocoon, the acidity and total hardness seemed to increase as the acidity of the water increased, but M-alkalinity was nutralized at 20~40 ppm of water acidity or the M-alkalinity could not be found in case over 40ppm of acidity. 7. In case increase of iron component with sample water, sericin solubility seemed to drop down, and mangan component showed the same nature but dull drop. 8. After cocoon was treated with water, acidity, M-alkalinity and total hardness were increased by the extraction from cocoon shell because of pH and treating temperature but not because of iron componnent. Mangan component, however, affected as to increase of acidity and total hardness but to decrease for M-alkalinity. 9. In case change of M-alkalinity and total hardness, sericin solubility has increased also. 10. In case constant pH and total hardness, the more M-alkalinity is, the more sericin solubility was found. 11. In case constant pH, total hardness, and M-alkalinity, the more acidity is, the less sericin solubility was found. 12. In case constant pH(6.8) and M-alkalinity, the more total hardness is, the less sericin solubility was found. 13. Though the combination of water, high solubility water, medium solubility water and low solubility water were prepared. The high solubility water desolved sericin 2.2% more than low solubility water. And the medium solubility water desolved sericin as much as 2.4~2.9%. 14. It was found that the most important factors for filature water are pH, M-alkalinity, acidity and total hardness which may need more words for optimum filature water development. 15. In case of repeat use of water, the buffer action of water has increased so that the sericin solubility to be decreased.

• Applied Microscopy
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• v.24 no.4
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• pp.61-77
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• 1994

The calcium-binding proteins (CaBP), parvalbumin (PV) and calbindin-D 28K (calbindin) are particularly abundant and specific in their distribution, and present in different subsets of neurons in many brain regions. Although their physiological roles in the neurons have not been elucidated, they are valuable markers of neuronal subpopulations for anatomical and developmental studies. This study is designed to characterize dorsal lateral geniculate nucleus (dLGN) neurons and axon terminals in terms of differential expression of immunoreactivity (IR) for two well-known CaBPs, PV and calbindin. The experiments were carried out on 6 adult monkeys. Monkeys were perfused under deep Nembutal anesthesia with 2% paraformaldehyde and 0.2% glutaraldehyde in 0.1M phosphate buffer. After removal, the brains were postfixed for 6-8 hr in 2% paraformaldehyde at $4^{\circ}C$ and infiltrated with 30% sucrose at $4^{\circ}C$. Thereafter, they were frozen in dry ice. Serial sections of the thalamus, at $20{\mu}m$, were made in the frontal plane with a sliding microtome. The sections were stained for PV and calbindin with indirect immunocytochemical methods. For electron microscopy, after infiltration with 30% sucrose the blocks of thalamus were serially sectioned at $50{\mu}m$ with a Vibratome in the coronal plane and stained immediately by indirect ABC methods without Triton X-100 in incubation medium. Stained sections were postfixed in 0.2% osmium tetroxide, dehydrated and flat-embedded in Spurr resin. The block was then trimmed to contain only a selected lamina or interlaminar space. The dLGN proper showed strong PV IR in fibers in all laminae and interlaminar zones. Particularly dense staining was noted in layers 1 and 2 that contain many stained fibers from optic tract. Neuronal cell body stained with PV was concentrated only in the laminae. In these laminae staining was moderate in cell bodies of all large and medium-sized neurons, and was strong in cell bodies of some small neurons together with their processes. Calbindin IR was marked in the neuronal cell body and neuropil in the S layers and interlaminar zones whereas moderate in the neuropil throughout the nucleus. Regional difference in distribution of PV and calbindin IR cell is distinct; the former is only in the laminae and the latter in both the S layer and interlaminar space. The CaBP-IR elements were confined to about $10{\mu}m$ in depth of Vibratome section. The IR product for CaBP was mainly associated with synaptic vesicle, pre- and post-synaptic membrane, and outer mitochondrial membrane and along microtubule. PV-IR was noted in various neuronal elements such as neuronal soma, dendrite, RLP, F, PSD and some myelinated or unmyelinated axons, and was not seen in the RSD and glial cells. Only a few neuronal components in dLGN was IR for calbindin and its reaction product was less dense than that of PV, and scattered throughout cytoplasm of soma of some relay neurons, and was also persent in some dendrite, myelinated axons and RLP. The RSD, F, PSD and glial elements were always non-IR for calbindin. Calbindin labelled RLP were presynaptic to unlabeled dendrite or dendritic spine and PSD. Calbindin-labeled dendrite of various sizes were always postsynaptic to unlabeled RSD, RLP or F. From this study it is suggested that dLGN cells of different functional systems and their differential projection to the visual cortex can be distinguished by differential expression of PV and calbindin.

• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.366-377
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• 2002

This study investigated the ecological environment of Dongguneung, which royal tombs of the Joseon Dynasty aye in. The aim of this study is to do an effective preservation, management and restoration of the royal tomb and garden of the Joseon Dynasty distributed in Seoul and Kyunggi Province through using the data of Dongguneung. In general, Dongguneung contains the predominant Oak class such as Quercus serrata-Quercus mongolica community, while a flatland surrounding its control office, which is often flooded with the rainy season in summer, is mainly Alnus japonica community, Pinus densiflora community ranges around the royal tomb. The subcommunity of Quercus serrata -Quereus mongolica community is distributed into Robinia pseudo-acacia, Pinus rigida, Pinus koraiensis, Carpinus laxiflora and typical subcommunity and so on. In particular, Robinia pseudo -acacia, Pinus rigida and Pinus koraiensis subcommunity, and Alnus japonica community were forested. The soil class of Dongguneung was mainly a sandy loam and its pH was an average of 4.67 (from 4.36 to 5.68). The content of heavy metals including Cu, Pb and Zn etc. in the soil was about twice as much as the natural content in the forest soil. The content of organism and total nitrogen in the topsoil layer was the average of 4.87% and 0.21% respectively, slightly higher than those (organism； 4.55%, total nitrogen； 0.20%) of the forest soil generated from granite bedrock. Cation exchange capacity as the indicator of soil fertility was 15.0 cmol $kg^{-1}$, higher than that in the granite forest soil. However among base exchangeable cations, contents of $Ca^{2+}$ (2.07 cmol $kg^{-1}$), $Mg^{2+}$ (0.40 cmol $kg^{-1}$) and K+ (0.25 cmol $kg^{-1}$) were slightly lower than that. The above results could reflect the need of soil fertilization and liming for the improvement of nutritional status and buffer process.

• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.498-511
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• 2012

Mountainous wetland have many species such as II grade endangered species of wild flora and fauna(Drosera rotundifolia) and environmental indicator species(Utricularia racemosa, Habenaria linearifolia, Parnassia palustris, Molinia japonica, etc.). Accordingly, the mountainous wetlands is very important. However, most mountainous wetlands will disappear by natural or artificial aridness processes. Thus, it needs to manage mountainous wetland for protecting from aridness. This study has found out the wetland status of the environmental ecology and aridness processes moreover, it has suggested ways of improving wetland conservation plan and wetland aridness management plan. According to the results of topography structure survey, Hwaeom wetland's altitude is ranged within 750~810m(87.4%), and slope is less than $10^{\circ}$. There was ideally suited mountainous wetland. However, the water supply(1.6 meters depth and 0.8 meters wide) was built on under the wetland. For that reason, there was concerned about the aridness processes by sweeping away peat layer and dropping the water level. The distribution area of hygrophyte was narrowed to 6.7% whereas, woody plants and xerophytic plants was achieved a dominant position. If it leaves the situation as it is, the mountainous wetland will be developed next succession as forest ecosystem. Therefore, in order to sustain the mountainous wetland from aridness, it is set to the base direction of conservation and management as main schemes. Moreover, we have suggested that setting the vegetation conservation and management area which considering a ecological vegetation characteristics, managing the ecotone vegetation, setting the buffer zone for protection of ecological core areas, protecting the mountainous wetland indicator species and designating the management vegetation. In conclusion, in order to sustain and maintain a soundly wetland ecosystem, it needs to several management of wetlands damage factors. 1) suppression of the excessive groundwater to basin, 2) stabilization of wetland via hydrologic storage, 3) suppression of changing and transforming wetland into forest by succession via management of xerophytic plants.

• Applied Microscopy
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• v.15 no.2
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• pp.31-68
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• 1985

Authors are trying to unveil the ultrastructural organization of olfactory bulb, which has been summerized under light microscopic level or communicated only in some detail in different view point until now. For the critical point of view, since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases (Brodal, 1969), the present study was carried out light and electron microscopic analyses of the structures of the neurons and synaptic organizations in olfactory bulbs from different animals in phylogenetical scale. We selected each one species from five animal classes: the house rabbit(Oryctolagus cuniculus var. domesticus [Gmelin]) from Mammalia, the domestic fowl (Gallus gallus domesticus Brisson) from Aves, the viper (Agkistrodon hylys [G.P. Pallas]) from Reptilia, a frog (Bombiana orientalis Boulenger) from Amphibia and the crussian carp (Carassius carassius [Linne]) from Pisces. For light microscopic study, samples were fixed in 10% formalin and paraffin sections were stained with hematoxylin-eosin. For the electron microscopic study, the tissues were fixed by perfusion through the heart or immersion with 1% paraform-aldehyde-glutaraldehyde mixture (phosphate buffer, pH 7.4), and final tissue block trimmed under dissecting microscope were osmicated (1% OsO4), they were embedded in Araldite or Epon 812, and ultrathin sections were made by LKB-V ultratome following the inspection of semi-thin sections stained with toluidine blue-borax solution. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100CX electron microscope. We have summerized our morphological analyses as follows: 1. The olfactory bulb of rabbit, viper and frog shows the eight layers of fila olfactoria, glomerular, external granular, external plexiform, mitral cell, internal plexiform, internal granular, medullary but domestic fowl shows the five layers of glomerular, fibrillar, mitral, granular and medullary and the three layers of fibrilla, glomerular and medullary in crussian carp. The sharpness of demarcation between the layers shows deferential tendency according to phylogenetical order. 2. Mitral cells of vertebrate have large triangular or oval shape with spherical nuclei which contain not so much chromatin. The cytoplasm contains numerous cell organelles, of which Nissl's bodies or granular endoplasmic reticula arranged as parallel strands. Development of granular endoplasmic reticula were declined as the phylogentical grade is going lower. 3. Tufted cells of all animal are mostly spindle or polygonal contour and contain oval nuclei which located in periphery of cytoplasm. The nuclei of rabbit, fowl, viper and frog has relatively space chromatin, but a nucleus of crussian carp contain irregularly aggregated chromatin in karyoplasm. Their cytoplasmic volume and cell organelle contents are in between those of mitral cell and granular cell. They contain moderate amount of mitochondria, granular endoplasmic reticula, a few Golgi complex, polysomes, lysosome, etc. 4. Granule of cells of all the vertebrate amimals studied exhibit similar features; cells and their dense nuclei show spherical or oval contour, and they have the thin rim of cytoplasm which contain only a few cell organelles. 5. In rabbit, the soma of mitral cells were in contact with boutons with two types of synaptic vesicles, that is, round and flat vesicles, especially flat vesicles in boutons were showing reciprocal synapses. However, in domestic fowls, vipers, frogs and crussian carps, there were found boutons showing only spherical synaptic vesicles. 6. The boutons containing round synaptic vesicles were made contact with the some of tufted cell of olfactory bulb in the rabbits, fowls, vipers and frogs, but no synaptic boutons were observed in soma of tufted cells in crussian carps. In the frogs, there were observed dendrites were contact with the soma of tufted cells. 7. In the neuropils of plexiform, granular and glomerular layers olfactory bulbs in the vertebrate, the synapses were axo-large dendrites, axo-median and small dendrites, dendrodendritic, and axo-axonal contacts. However, in the neuropil of crussian carps, synapses were observed only in glomerular layer.

• Journal of Life Science
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• v.33 no.4
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• pp.357-362
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• 2023

This report looks at an agar-degrading marine bacterium and characterization of its agarase. Agar-degrading marine bacterium JS-1 was isolated with Marine agar 2216 media from seawater from the seashore of Sojuk-do, Changwon in Gyeongnam Province, Korea. The agar-degrading bacterium was named as Agarivorans sp. JS-1 by phylogenetic analysis based on 16S rRNA gene sequencing. The extracellular agarase was prepared from the culture media of Agarivorans sp. JS-1 and used for characterization. Relative activities at 20℃, 30℃, 35℃, 40℃, 45℃, 50℃, 55℃, and 60℃ were 70%, 74%, 78%, 83%, 87%, 100%, 74%, and 66%, respectively. Relative activities at pH 5, 6, 7, and 8 were 91%, 100%, 90%, and 89%, respectively. Its extracellular agarase showed maximum activity (207 units/l) at pH 6.0 and 50℃ in 20 mM Tris-HCl buffer. The residual activity after heat treatment at 20℃, 30℃, and 50℃ for 30 minutes was 90%, 70%, and 50% or more, respectively. After a 2-hour heat treatment at 20℃, 30℃, 35℃, 40℃, and 45℃, the residual activity was 80%, 68%, 65%, 63%, and 57%, respectively. At 50℃ and above, after heat treatment for 30 minutes, the residual activity was below 60%. Thin layer chromatography analysis suggested that Agarivorans sp. JS-1 produces extracellular β-agarases as they hydrolyze agarose to produce neoagarooligosaccharides such as neoagarohexaose (20.6%), neoagarotetraose (58.5%), and neoagarobiose (20.9%). Agarivorans sp. JS-1 and its thermotolerant β-agarase would be useful in the production of neoagarooligosaccharides, showing functional activity such as inhibition of bacterial growth and delay of starch degradation.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.32 no.1
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• pp.93-106
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• 2014

In this study, Analyze environment of location, investigation into vegetation resources, survey management status and establish to classify the management area for Natural monument No.374 Pyengdae-ri Torreya nucifera forest. The results were as follows: First, Torreya nucifera forest is concerned about influence of development caused by utilization of land changes to agricultural region. Thus, establish to preservation management plan for preservation of prototypical and should be excluded development activity to cause the change of terrain that Gotjawal in the Torreya nucifera forest is factor of base for generating species diversity. Secondly, Torreya nucifera forest summarized as 402 taxa composed 91 familly 263 genus, 353 species, 41 varieties and 8 forms. The distribution of plants for the first grade & second grade appear of endangered plant to Ministry of Environment specify. But, critically endangered in forest by changes in habitat, diseases and illegal overcatching. Therefore, when establishing forest management plan should be considered for put priority on protection. Thirdly, Torreya nucifera representing the upper layer of the vegetation structure. But, old tree oriented management and conservation strategy result in poor age structure. Furthermore, desiccation of forest on artificial management and decline in Torreya nucifera habitat on ecological succession can indicate a problem in forest. Therefore, establish plan such as regulation of population density and sapling tree proliferation for sustainable characteristics of the Torreya nucifera forest. Fourth, Appear to damaged of trails caused by use. Especially, Scoria way occurs a lot of damaged and higher than the share ratio of each section. Therefore, share ratio reduction Plan should be considered through the additional development of tourism routes rather than the replacement of Scoria. Fifth, Representing high preference of the Torreya nucifera forest tourist factor confirmed the plant elements. It is sensitive to usage pressure. And requires continuous monitoring by characteristic of Non-permanent. In addition, need an additional plan such as additional development of tourism elements and active utilizing an element of high preference. Sixth, Strength of protected should be differently accordance with importance. First grade area have to maintenance of plant population and natural habitats. Set the direction of the management. Second grade areas focus on annual regeneration of the forest. Third grade area should be utilized demonstration forest or set to the area for proliferate sapling. Fourth grade areas require the introduced of partial rest system that disturbance are often found in proper vegetation. Fifth grade area appropriate to the service area for promoting tourism by utilizing natural resources in Torreya nucifera forest. Furthermore, installation of a buffer zone in relatively low ratings area and periodic monitoring to the improvement of edge effect that adjacent areas of different class.