• Molecular & Cellular Toxicology
• /
• v.3 no.1
• /
• pp.19-22
• /
• 2007

Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

• Journal of Ecology and Environment
• /
• v.30 no.2
• /
• pp.143-149
• /
• 2007

To certify predictability for the times of phenological stages from cumulative air temperature in springtime, the first times of budding, leafing, flower budding, flowering and deflowering for 14 woody plants were monitored and air temperature was measured from 2005 to 2006 at Namsan. Year day index (YDI) and Nuttonson's Index (Tn) were calculated from daily mean air temperature. Of the 14 woody species, mean coefficient of variation was 0.04 in Robinia pseudo-acacia and 0.09 in Alnus hirsuta. However, mean coefficient of variation was 0.30 in Forsythia koreana and Stephanandra incisa and 0.32 in Zanthoxylum schinifolium. Therefore, the times of each phenological stage could be predicted in the former two species but not in latter three species by two indices. Of the five phenological stages, mean coefficient of variation was the smallest at deflowering time and the largest at budding time. In five phenological stages, mean coefficient of variation of YDI was in the range of $0.11{\sim}0.21$ but that of Tn was in the range of $0.15{\sim}0.26$. Therefore, the former was a better index than the latter. Of the species-phenological stage pair, coefficient of variation of YDI was 0.01 in Acer pseudo-sieboldianum - flower budding and below 0.05 in 11 pairs, whereas the YDIs over 0.40 were 4 pairs comprising of Prunus leveilleana - budding (0.51). Coefficient of variation of Tn was 0.01 in A. hirsuta - budding and below 0.05 in 8 pairs. The Tns over 0.40 were 5 pairs comprising of F. koreana - flower budding (0.66).

• Journal of Sericultural and Entomological Science
• /
• v.29 no.1
• /
• pp.12-19
• /
• 1987

This study was carried out to investigate the effects of climatic conditions on the budding of buds and the growth of shoots of mulberry (Morus alba L.) at Suwon for the period of six years from 1978 to 1983. The results are summarized as follows. 1. The period that influenced most greatly on mulberry budding and foliating of it's buds was from late part of March to middle part of April in the district of Suwon, Korea. 2. Temperatures in soil 20cm deep during the period from late part of March to middle part of April had high correlations with budding dates. 3. It was disclosed that the budding date had high correlations with the date of bud shaped swallow-bill (r=0.9861**), date of the third leaf(r=0.97**), and date of the fifth leaf(r=0.96**), respectively. 4. The higher the average temperature of April became, the longer the length of shoots became. 5. The earlier the budding date and foliating date came, the larger the leaf yield became. However, with excessive amount of precipitation after early budding and foliating, the leaf yield was not increased in proportional. 6. The longer the growing period of the shoots became, the larger the leaf yield became.

• The Korean Journal of Mycology
• /
• v.6 no.1
• /
• pp.21-27
• /
• 1978

The growing behavior of Candida albicans in various concentration of glucose and corticosteroid media was studied with the method of modified hanging slide culture. The strains of Candida albicans used in this study were obtained from vaginal swab from outpatients and were isolated from cultured colonies on Sabouraud's glucose agar media. To detect the budding rate of blastospore, the diluted suspension of Candida albicans in normal saline were inoculated into various concentration of glucose (Gl, G2, G3, G4), corticosteroid (S1, S2, S3, S4) and corticosteroid with 10% pepton broth (D1, D2, D3, D4) respectively and cultured at room temperature $(22{\sim}25^{\circ}C)$. The number of budding of blastospore were counted under the high power field of light microscope (400X) at specific time interval, e.g, 1, 2, 3, 6, 9, 12, 18, and 24 hours after inoculation. The results are as following: 1. The most effective budding rate was seen in G4 media (1.25% glucose) in 18 hours aft inoculation (89%). 2. The budding rate in Sabouraud's glucose broth with various concentration of dexamethasone added, was not significantly different from that of simple Sabouraud's glucose broth within 18 hours after inoculation, but there was statistically. significant difference in two budding rate at 24 hours observation. 3. The budding rate in 10% pepton broth media with various concentration of dexamethasone was almost same budding rate in control group, which is normal saline and 10% pepton broth, except on 2 and 24 hours results.

• KSBB Journal
• /
• v.29 no.5
• /
• pp.336-342
• /
• 2014

Asexual benthic polyp reproduction plays a major role in the jellyfish bloom. Recent studies found that temperature is the most important factor to regulate the budding rate of the polyps. We established a simple dynamic model to count the number of polyps depending on the variation of temperature with two data sets from different places. The population of polyps was counted through the budding rate and the number of budding times by Fibonacci sequence. It is assumed that the budding rate depends on the temperature only. The budding rate of the asexual reproduction shows very sensitive to the distribution of the seawater temperature. The model was tested to the temperature data of Ansan located in the west sea of Korea. The results indicate that this model can be useful to predict the blooms of Aurelia aurita polyps, which may have considerable influence on the bloom of medusa. The shape of temperature curve plays a key role in the predicting the bloom of Aurelia aurita polyps.

• Journal of Life Science
• /
• v.9 no.1
• /
• pp.22-25
• /
• 1999

In order to study the mechanism for the adaptation to salt stress, we mutagenized budding yeast Saccharomyces cerevisiae with Ethylmethane sulfonate, and isolated salt-tolerant mutants. Among the salt-tolerant mutants, two strains exhibit additional temperature sensitive phenotype. Here, we report that these two salt-tolerant mutants are specific to {TEX}$Na^{+}${/TEX} rather than general osmotic stress. These mutant strains may contain mutations in the genes involved in {TEX}$Na^{+}${/TEX} home-ostasis.

• Horticultural Science & Technology
• /
• v.17 no.3
• /
• pp.329-332
• /
• 1999

The objective of this study was to determine the influence of grafting timing, rootstock cut timing and leaf removal with promalin ($GA_{4+7}+BA$) treatments on the maiden tree growth, lateral development and flower bud initiation. In mid-March 1997, two-year-old M.9-T337 rootstocks selected with trunk diameter over 1 cm were planted in the field. Chip budding with 'Fuji' scion on M.9-T337 rootstock budded in mid-April was earlier in sprouting than chip budding in mid-June. Late cutting chip budding (LCCB) with 'Fuji' scion on M.9-T337 rootstock was lower in the failed budding percentage with 14% than that of early cutting chip budding (ECCB). Especially, ECCB in April was not suitable for scion growth such as uniformity with high percentage of failed tree. Grafting timing in mid-June and rootstock cutting timing of LCCB induced more branches and flower buds than other treatments. Removal of 8 to 10 uppermost immature leaves on central leader stem and application of Promalin 250 mg/L after every 30 cm of terminal growth produced a 189 cm tall tree with 9 flower buds and 14.2 short lateral shoot from 30 to 35 cm long in length in 1998. Promalin increased branching on second-season growth and, when combined with leaf removal, resulted in uniform distribution of branches along the central leader stem.

• Journal of Microbiology
• /
• v.40 no.3
• /
• pp.245-247
• /
• 2002

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

• Journal of Ecology and Environment
• /
• v.31 no.2
• /
• pp.139-146
• /
• 2008

To clarify the relationship between the timing and the duration of flowering among populations, plants, and individual flowers, the dates of flower budding, flowering and deflowering were monitored for ten woody species from March 1 to June 30, in 2005, 2006 and 2007, in temperate deciduous forests at three sites of Namsan, and individual plants from seven woody species were monitored from March 1 to May 31, in 2006. Total durations of flower budding, flowering, and deflowering varied among the plant species. Three durations of these phenological stages of Stephanandra incisa were the longest (74 days, 109 days, and 101 days, respectively), and those of Prunus serrulata var. spontanea were the shortest (7 days, 7 days, and 4 days, respectively). For each species, phenological durations varied among years but were similar among the study sites in the same year. There was no relationship between flowering time and flowering duration on the population level. On the plant level, the duration of flower budding was over 11 days in all specie; S. incisa had the longest duration (73.3 days), and that of Styrax japonica was long as well (29.0 days), while that of Prunus leveilleana was the shortest (11.3 days). The longer the mean flower budding duration, the greater the difference among the plants within a species. The flowering duration of for S. incisa was 92.2 days, while that of Forsythia koreana was 27.2 days. The flowering durations of all other species were $10{\sim}20$ days. The deflowering duration was 92.0 days in S. incisa and <15 days in all other species. Differences among the plants in deflowering duration were smaller than those of the other phenological stages. In the species that flowered in April, the correlation coefficient between the flowering duration and the first flowering date was negative and significant. However, in the species that flowered in May, the correlation between flowering duration and the first flowering date was not significant. For individual plants of all species except for S. alnifolia, the earlier the flowering time, the longer the flowering duration. Differences between flowering time and flowering duration across years were significant in six species.

• Journal of Sericultural and Entomological Science
• /
• v.5
• /
• pp.1-5
• /
• 1965

The purpose of this work is to investigate the effects of picking leaves in autumn on the non-budding and survival of grafting. The results are as follows. 1) The leaf picking in autumn causes an increase of the non-budding ratio in the following spring. When tue whole leaves picking is compared with the control, the non-budding ratio increases 46 percent. In case of tone leaves on a single stem are picked in various tray, the non-budding ratio of picked portion is higher than that of non-picked. 2) The leaf picking in autumn with winter bud being grafted on stock in the next spring, decrease the survival of grafting. In case some leaves on a single stem are picked in various way, the winter bud of picked part is always poor in survival. 3) In the event of leavf picking in autumn, the yield always decreases 7∼20 percent, especially this phenomenon becomes serious in the under half part of the stem(20%) and whole leaves picking(12％) treatments.