Isolation and Characterization of Salt Tolerant Mutations in Budding Yeast Saccharomyces cerevisiae

  • Kim, Yung-Jin (Department of Molecular Biology, Pusan National University) ;
  • Seo, Soo-Boon (Department of Molecular Biology, Pusan National University) ;
  • Park, Shi-Young (Department of Molecular Biology, Pusan National University)
  • Published : 1999.06.01

Abstract

In order to study the mechanism for the adaptation to salt stress, we mutagenized budding yeast Saccharomyces cerevisiae with Ethylmethane sulfonate, and isolated salt-tolerant mutants. Among the salt-tolerant mutants, two strains exhibit additional temperature sensitive phenotype. Here, we report that these two salt-tolerant mutants are specific to {TEX}$Na^{+}${/TEX} rather than general osmotic stress. These mutant strains may contain mutations in the genes involved in {TEX}$Na^{+}${/TEX} home-ostasis.

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