• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.286-290
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• 2008

Many important traits have been tagged allowing plant breeders to apply marker assisted selection (MAS) in rice. PCR itself is simple to set up, and requires little hands-on time. However, a crucial limiting step of MAS programs is the reliable and efficient extraction of DNA which can be performed on thousands of individuals. In this study, We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract DNA from leaf tissues for suitable MAS in rice. We followed the standard 2% CTAB extraction method in all the procedure. In addition we used the 1.2 ml 8-strip tube instead of 1.5 ml E-tubes to fit the 8-multichannel pipette and employ the 96 well plate to use the swing bucket centrifuge. Our modified CTAB DNA extraction method offers several advantages with respect to traditional and simple methods. 1) adult leaf samples collected in paddy field are applicable. 2) 96 leaf samples can be homogenized only one-time by using tungsten carbonate bead and 96well block. 3) semiautomatic loading method using 8-multichannel pipette from DNA extraction to electrophoresis of PCR products. 4) our system can extract about 400 leaf samples per day by only one technicion. Therefore, this method could be useful for marker assisted breeding in rice.

• Transactions of the KSME C: Technology and Education
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• v.3 no.2
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• pp.131-140
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• 2015

The mono-ski for the paraplegia designed to skiing is formed as seat bucket on the sled. The impact force transferred by snow surface during skiing is absorbed by the leg joints of normal human, but it is transferred to the human body on the seat when using mono-ski. Most of commercially available mono-ski have absorbing device and link mechanism between seat and ski mount in order to complement it. In this study we developed the comfort evaluation model that could provide skiing simulation of mono-ski with hydraulic damper and analyzed vibrational acceleration occurred during skiing uneven surface. The evaluation method used in this study is the international standard BS6841. We evaluated comfort performance of mono-ski in accordance with nozzle adjustment of hydraulic damper.

• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.4
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• pp.19-27
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• 2012

The objective of this study was to test the pollutant reduction effect by the silt fence and vegetated ridge through field monitoring. The experiment plots were established with two replication and three treatments. Each plot was designed with 5 m width, 22 m length, and 3 % slope. Flumes with the floating type stage gages were installed at the outlet of each plot to monitor the runoff. For a rainfall monitoring, tipping bucket rain gage was installed within the experiment site. Cultivated crops were spring daikon and autumn chinese cabbage. Analysis of variance (ANOVA) indicated that the water quality concentration from three experiment plots were not significantly different in 5 % of significant level. The monitoring results showed that the average pollution loads reduced by silt fence compared to control were SS 75.33 %, TN 40.87 %, TP 56.58 %, BOD 52.12 %, COD 36.07 %, TOC 34.99 %; by vegetated ridge compard to control were SS 65.27 %, TN 81.80 %, TP 54.26 %, BOD 67.09 %, COD 46.55 %, TOC 43.30 %. Analysis of Spearman's rank correlation coefficient showed that BOD-SS and SS-Turbidity were highly related at the silt fence and vegetated ridge plots. In all plots, SS-Turbidity and TP-TN relations were relatively high. The monitoring results showed that the silt fence and vegetated ridge were effect method to reduce the pollutant loads from the field runoff. Long-term monitoring is required to obtain more quantitative reduction effect for diverse crops and to increase the reliability of results.

• KIEAE Journal
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• v.5 no.2
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• pp.11-18
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• 2005

The Purpose of this study is development and analysis of Effluence Characteristic of the Rainfall in the IRMA Green Roof System(developed in KICT) Plus 50 program is an internal research project at KICT(Korean Institute of Construction Technology) which has it as an object ; to lengthen the building's life 50-year or more and reduce energy conception 50% than present. Green roof system is one of the most important theme in the Plus 50 program. Generally, a Green Roof System has a positive effect on the thermal conductivity in winter, the micro cooling effect on building and city by evaporation in summer, the flood-control effect by runoff-reduction or the treated rainwater-quality of green roof system and so on. However, inspection of the physical effect of green roof system does not consider in Korea. Above all, long-term monitoring and a whole observation of green roof system is needed to probate the effect. So a new experimental method could be tried in this research, which is never attempted in Korea. The measurement by a bucket with a great volume, 1L, gives a new dimension of measuring green roof effect to measure the permanent running flood from a wide roof. This offers a reasonable result on a long-term measuring of a running water. Additionally, the thermal behavior of the IRMA(Insulated Roof Membrane Assembly), known in the western europe as a reasonable solution at green roof system by economical benefits and easy construction, would be experimented.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.23 no.6
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• pp.1259-1266
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• 2013

Nowadays, the databases are getting larger and larger. As the company has difficulty in managing the database, they want to outsource the database to the cloud system. In this case the database security is more important because their database is managed by the cloud service provider. Among database security techniques, the encryption method is a well-certified and established technology for protecting sensitive data. However, once encrypted, the data can no longer be easily queried. The performance of the database depends on how to encrypt the sensitive data, and on the approach for searching, and the retrieval efficiency that is implemented. In this paper we propose the new suitable mechanism to encrypt the database and lookup process on the encrypted database under control of the cloud service provider. This database encryption algorithm uses the bloom filter with the variable keyword based index. Finally, we demonstrate that the proposed algorithm should be useful for database encryption related research and application activities.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.11
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• pp.2745-2752
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• 2013

The tipping-bucket and weight measuring type precipitation gauge has long been used worldwide for measuring rainfall. However, the conventional gauge has observation errors and its measurement range is limited by the device's resolution. In this paper, a new type of precipitation gauge that uses an innovative method by applying a new ultrasonic flow measuring technique was developed. This is the first time this technique is being used to gauge rainfall. The prototype was tested in the laboratory designated by the Korea Laboratory Accreditation Scheme (KOLAS). The rainfall intensity condition was 20~420 mm/H and the Standard Correction System for Precipitation Gauges was used. Results of the laboratory experiment showed that the proposed gauge has a ${\pm}2%$ margin of error. Consequently, it was proven that the proposed gauge is quite accurate and reliable for measuring precipitation.

• Korean Journal of Veterinary Service
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• v.17 no.3
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• pp.208-226
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• 1994

From January to December 1993, 3,385 bulk milk samples were collected from 293 herds in Inchon area. Standard plate counts(SPC) and somatic cell counts(SCC) were performed by the method of milk collection, the situation of raising management and monthly. The results obtained are summarized as follows : 1, Annual average SPC and SCC were respectively 638,000 cfu /mm and 647,000 cells /mm. SPC showed an abrupt decrease from January-1,088,000 cfu /mm to December-279,000 cfu /mm, but SCC showed a slow change from January-1,017,000 cells /mm to December -673,000 cells /mm 2. Variation on milk quality(annual average SPC) was shown a wide difference between everyday collection-575,000 cfu /mm and every other day collection-1,243,000 cfu /mm ac-cording to frequency of milk collection from dairy farms. However, there was a little difference In SCC. 3. In the raising scale, average SPC were the lowest in 16~25mi1king cows, and average SCC were the lowest in above 25milking cows. 4. According to types of milking machine, average SPC and SCC of dairy farms that are equipped with pipeline system were respectively 361,000 cfu /mm and 591,000 cells /mm. Those of dairy farms with bucket system were 549,000 cfu /me and 559,000 cells /mm. 5. In the types of management, average SPC an SCC of dairy farms with hired herdsman were 288,000 cfu /mm and 559,000 cells /mm. Those of dairy farms with self-management were 526,000 cfu /mm and 568,000 cells /mm.

• Journal of the Korean Geotechnical Society
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• v.36 no.10
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• pp.5-19
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• 2020

With increasing demand for offshore structures, the demand for temporary structures to help the offshore construction work has increased. A cofferdam is a temporary barrier to stop the inflow of water in the construction site and allows working in the dry condition when the construction is done within the water. However, it is a major cause of construction delays and increased costs because additional works are required to block the water inflow. Recently, in order to overcome the limitations of the conventional cofferdam methods and to increase economic efficiency, a large-diameter steel cofferdam method has been proposed which can be installed quickly in the seabed by using the suction pressure. In this circular steel cofferdam method, the top side of the cofferdam including the top-lid is always exposed above the sea level in order to use it as a water barrier, unlike the conventional suction bucket foundation. After installation, the top-lid of the cofferdam is removed and the water filled inside the cofferdam is discharged to make the interior dry condition. In this study, the circular steel cofferdam with a 5 m inner diameter was fabricated and the installation tests were conducted at the Saemaguem test site. During the experiment, variation of suction pressure, leakage between connections, structure deformation, and inclination of the steel cofferdam were measured and post-analyzed. This study verified the new circular steel cofferdam method and confirmed that the suction installation method can be successfully used for various purposes on offshore structures.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.240-240
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• 2011

The first neutral beam injector (NBI-1) has been developed for the Korea Superconducting Tokamak Advanced Research (KSTAR) tokamak. A first long pulse ion source (LPIS-1) has been installed on the NBI-1 for an auxiliary heating and current drive of KSTAR core plasmas. Performance of ion and neutral beam extractions in the LPIS-1 was investigated initially on the KSTAR NBI-1 system, prior to the neutral beam injection into the main plasmas. The ion source consists of a JAEA magnetic bucket plasma generator with multi-pole cusp fields and a set of KAERI prototype-III tetrode accelerators with circular apertures. The inner volume of plasma generator and accelerator column in the LPIS-1 is approximately 123 liters. Final design requirements for the ion source were a 120 kV/ 65 A deuterium beam and a 300 s pulse length. The extraction of ion beams was initiated by the formation of arc plasmas in the LPIS-1, called as an arc-beam extraction method. A stable ion beam extraction of LPIS-1 has been achieved up to an 100 kV/42 A for a 4 s pulse length and an 80 kV/25 A for a 14 s pulse length. Optimum beam perveance of 1.21 microperv has been found at an accelerating voltage of 80 kV. Neutralization efficiency has been measured by using a water flow calorimetry (WFC) method of calorimeter and an operation of bending magnet. The full-energy species of ion beams have been detected by using the diagnostic method of optical multichannel analyzer (OMA). An arc efficiency of the LPIS was 0.6~1.1 A/kW depending on the operating conditions of arc discharge.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.35C no.10
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• pp.69-77
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• 1998

This paper presents a new efficient and stable multilevel partitioning algorithm for VLSI circuit design. The performance of multilevel partitioning algorithms that are proposed to enhance the performance of previous iterative-improvement partitioning algorithms for large scale circuits, depend on choice of construction methods for partition hierarchy. As the most of previous multilevel partitioning algorithms forces experimental constraints on the process of hierarchy construction, the stability of their performances goes down. The lack of stability causes the large variation of partition results during multiple runs. In this paper, we minimize the use of experimental constraints and propose a new method for constructing partition hierarchy. The proposed method clusters the cells with the connection status of the circuit. After constructing the partition hierarchy, a partition improvement algorithm, HYIP$^{[11]}$ using hybrid bucket structure, unclusters the hierachy to get partition results. The experimental results on ACM/SIGDA benchmark circuits show improvement up to 10-40% in minimum outsize over the previous algorithm $^{[3] [4] [5] [8] [10]}$. Also our technique outperforms ML$^{[10]}$ represented multilevel partition method by about 5% and 20% for minimum and average custsize, respectively. In addition, the results of our algorithm with 10 runs are better than ML algorithm with 100 runs.