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Tag-SNP selection and online database construction for haplotype-based marker development in tomato (유전자 단위 haplotype을 대변하는 토마토 Tag-SNP 선발 및 웹 데이터베이스 구축)

  • Jeong, Hye-ri;Lee, Bo-Mi;Lee, Bong-Woo;Oh, Jae-Eun;Lee, Jeong-Hee;Kim, Ji-Eun;Jo, Sung-Hwan
    • Journal of Plant Biotechnology
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    • v.47 no.3
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    • pp.218-226
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    • 2020
  • This report describes methods for selecting informative single nucleotide polymorphisms (SNPs), and the development of an online Solanaceae genome database, using 234 tomato resequencing data entries deposited in the NCBI SRA database. The 126 accessions of Solanum lycopersicum, 68 accessions of Solanum lycopersicum var. cerasiforme, and 33 accessions of Solanum pimpinellifolium, which are frequently used for breeding, and some wild-species tomato accessions were included in the analysis. To select tag-SNPs, we identified 29,504,960 SNPs in 234 tomatoes and then separated the SNPs in the genic and intergenic regions according to gene annotation. All tag-SNP were selected from non-synonymous SNPs among the SNPs present in the gene region and, as a result, we obtained tag-SNP from 13,845 genes. When there were no non-synonymous SNPs in the gene, the genes were selected from synonymous SNPs. The total number of tag-SNPs selected was 27,539. To increase the usefulness of the information, a Solanaceae genome database website, TGsol (http://tgsol. seeders.co.kr/), was constructed to allow users to search for detailed information on resources, SNPs, haplotype, and tag-SNPs. The user can search the tag-SNP and flanking sequences for each gene by searching for a gene name or gene position through the genome browser. This website can be used to efficiently search for genes related to traits or to develop molecular markers.

Non-Marker Based Mobile Augmented Reality Technology Using Image Recognition (이미지 인식을 이용한 비마커 기반 모바일 증강현실 기법 연구)

  • Jo, Hui-Joon;Kim, Dae-Won
    • Journal of the Institute of Convergence Signal Processing
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    • v.12 no.4
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    • pp.258-266
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    • 2011
  • AR(Augmented Reality) technology is now easily shown around us with respect to its applicable areas' being spreaded into various shapes since the usage is simply generalized and many-sided. Currently existing camera vision based AR used marker based methods rather than using real world's informations. For the marker based AR technology, there are limitations on applicable areas and its environmental properties that a user could immerse into the usage of application program. In this paper, we proposed a novel AR method which users could recognize objects from the real world's data and the related 3-dimensional contents are also displayed. Those are done using image processing skills and a smart mobile embedded camera for terminal based AR implementations without any markers. Object recognition is done from the comparison of pre-registered and referenced images. In this process, we tried to minimize the amount of computations of similarity measurements for improving working speed by considering features of smart mobile devices. Additionally, the proposed method is designed to perform reciprocal interactions through touch events using smart mobile devices after the 3-dimensional contents are displayed on the screen. Since then, a user is able to acquire object related informations through a web browser with respect to the user's choice. With the system described in this paper, we analyzed and compared a degree of object recognition, working speed, recognition error for functional differences to the existing AR technologies. The experimental results are presented and verified in smart mobile environments to be considered as an alternate and appropriate AR technology.

Development and Clinical Evaluation of Wireless Gyro-mouse for the Upper Extremity Disabled to Use Computer (상지장애인의 컴퓨터 사용을 위한 무선 자이로마우스의 개발 및 임상평가)

  • Han Ha-Na;Song Eun-Beom;Kim Chul-Seung;Heo Ji-Un;Eom Gwang-Moon
    • Science of Emotion and Sensibility
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    • v.9 no.2
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    • pp.93-100
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    • 2006
  • This paper aims at the development and clinical evaluation of the wireless gyro-mouse system. The wireless gyro-mouse system is a computer interface with gyro-sensor and wireless communication, for the patients with upper-extremity disabled from the traffic accident or stroke to use the computer software i.e. internet browser. In the development, we focused on, firstly, to make the system wireless for the patients to manipulate the mouse easily even on the bed or wheelchair, secondly, to insert the gyro-sensor into a headband for easy don-and-doff and aesthetic appearance, thirdly, to devise a click switch in case of $C5{\sim}C6$ patients and a head nodding detection in case of C4 patients for sending click message to computer operating system. We performed evaluation experiment for patients with upper-extremities disabled from spinal cord injury. The results show that the displacement error of the cursor position against the target position during linear (vertical/horizontal) movement manipulation decreased with trial number. The click rate per minute also increased with trial number. This indicates the developed wireless gyro-mouse system would be more useful to the patients with repetitive use.

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A Study on the Link Server Development Using B-Tree Structure in the Big Data Environment (빅데이터 환경에서의 B-tree 구조 기반 링크정보 관리서버의 개발)

  • Park, Sungbum;Hwang, Jong Sung;Lee, Sangwon
    • Journal of Internet Computing and Services
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    • v.16 no.1
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    • pp.75-82
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    • 2015
  • Major corporations and portals have implemented a link server that connects Content Management Systems (CMS) to the physical address of content in a database (DB) to support efficient content use in web-based environments. In particular, a link server automatically connects the physical address of content in a DB to the content URL shown through a web browser screen, and re-connects the URL and the physical address when either is modified. In recent years, the number of users of digital content over the web has increased significantly because of the advent of the Big Data environment, which has also increased the number of link validity checks that should be performed in a CMS and a link server. If the link validity check is performed through an existing URL-based sequential method instead of petabyte or even etabyte environments, the identification rate of dead links decreases because of the degradation of validity check performance; moreover, frequent link checks add a large amount of workload to the DB. Hence, this study is aimed at providing a link server that can recognize URL link deletion or addition through analysis on the B-tree-based Information Identifier count per interval based on a large amount of URLs in order to resolve the existing problems. Through this study, the dead link check that is faster and adds lower loads than the existing method can be performed.

Building an SNS Crawling System Using Python (Python을 이용한 SNS 크롤링 시스템 구축)

  • Lee, Jong-Hwa
    • Journal of Korea Society of Industrial Information Systems
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    • v.23 no.5
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    • pp.61-76
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    • 2018
  • Everything is coming into the world of network where modern people are living. The Internet of Things that attach sensors to objects allows real-time data transfer to and from the network. Mobile devices, essential for modern humans, play an important role in keeping all traces of everyday life in real time. Through the social network services, information acquisition activities and communication activities are left in a huge network in real time. From the business point of view, customer needs analysis begins with SNS data. In this research, we want to build an automatic collection system of SNS contents of web environment in real time using Python. We want to help customers' needs analysis through the typical data collection system of Instagram, Twitter, and YouTube, which has a large number of users worldwide. It is stored in database through the exploitation process and NLP process by using the virtual web browser in the Python web server environment. According to the results of this study, we want to conduct service through the site, the desired data is automatically collected by the search function and the netizen's response can be confirmed in real time. Through time series data analysis. Also, since the search was performed within 5 seconds of the execution result, the advantage of the proposed algorithm is confirmed.

A Web-based System for Real-time Monitoring of Dangerous Objects using RFID (RFID를 이용한 웹 기반의 실시간 위험물 모니터링 시스템 구축 사례)

  • Kim, Ju-Il;Lee, Woo-Jin;Chong, Ki-Won
    • The Journal of Society for e-Business Studies
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    • v.13 no.2
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    • pp.101-115
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    • 2008
  • Recently in the human society, the crime using small arms has increased. Also, many accidents happen because of incomplete management of chemicals and radiation. Accordingly, it is necessary to manage dangerous objects by tracing the position of dangerous objects and rapidly providing the correct information for them. This paper presents a web-based system for real-time monitoring of dangerous objects using RFID in order to overcome the limitations and problems of current dangerous objects management techniques. In this paper, we define the architecture for web-based dangerous objects monitoring system and the scheme for storing information of a dangerous object in the RFID tag. We also implement the web-based monitoring system and present the execution result of the system. The proposed real-time monitoring system is composed of the dangerous objects monitoring server which manages information of dangerous objects and controls them, the dangerous objects monitoring middleware which is mediator between dangerous objects and the server, the RFID reader which reads information of dangerous objects from RFID tags attached to the objects and the database which stores information, status and position of dangerous object. The proposed system manages diverse dangerous objects such as small arms, radiation and harmful chemicals based on the position of them using RFID, so the user can check dangerous objects when they are checked in and checked out and the user can acquire the real-time position information of them through the system. Furthermore, the user can visually monitor dangerous objects through web browser from any where and at any time because the system is web-based system and it provides graphical user interface.

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The Effect of New Product Preannouncing Convergence Strategy on Attitude and Judgement Using Facebook Brand Fan Page (페이스북 브랜드 팬 페이지를 활용한 신제품 프리어나운싱 융합전략이 태도와 판단에 미치는 효과)

  • Han, Kwang-Seok;Cho, Jae-Hyun
    • Journal of the Korea Convergence Society
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    • v.8 no.3
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    • pp.163-168
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    • 2017
  • This study empirically examines the differences in attitude and memory effect according to the product knowledge (high knowledge / low knowledge) and temporal distance (near future / far future) in the new product preannouncing strategy of companies through Facebook Respectively. Experiments were conducted by inserting the experiment into a virtual web browser using Facebook 's actual brand fan page and then connecting the experiment to the experimental banner site. As a result of research, when preannouncing new products are released in the near future, low knowledge consumers have made judgments of purchasing decisions because GRM judgments are more favorable than property information (ARM) judgments, but preannouncing is a new product At the time of launch, highly-knowledgeable consumers had a positive effect on the property information (ARM) judgment of the product rather than the comprehensive information (GRM) judgment. In addition, when preannouncing was introduced in the near future, the attitudes of the products between the low-knowledge consumers and the high-knowledge consumers were all positive, but when the new products were launched in the distant future, Product attitude was negative.

A Rapid Region-of-Interest Processing Technique using Mask Patterns for JPEG2000 (JPEG2000에서 마스크 패턴을 이용한 빠른 관심영역 처리 기법)

  • Lee, Jum-Sook;Ha, Seok-Woon;Park, Jae-Heung;Seo, Yeong-Geon;Kang, Ki-Jun;Hong, Seok-Won;Kim, Sang-Bok
    • Journal of the Korea Society of Computer and Information
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    • v.15 no.6
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    • pp.19-27
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    • 2010
  • An region of interest processing technique is to handle preferentially some part of an image dynamically according to region of interest of the users in JPEG2000 image. A small image is not important, but in a big image the specified region that the user indicated has to be handled preferentially because it takes long time to display the whole image. If the user indicates a region of the outline image, the browser masks the region and sends the mask information to the source that transmitted the image. The server which got the mask information preferentially sends the code blocks matching the masks. Here, quickly generating mask information is important, so, in this paper using predefined 48 mask patterns, selecting one of the patterns according to the distribution of ROI(Region-of-Interest) and background, we remarkably reduced the time computing the mask region. Blocks that the patterns are applied are the blocks mixed of ROI and background in a block. If a whole block is an ROI or a background, these patterns are not applied. As results, comparing to the method that precisely handles ROI and background, the quality is unsatisfactory but the processing time remarkably reduced.

Welfare Interface using Multiple Facial Features Tracking (다중 얼굴 특징 추적을 이용한 복지형 인터페이스)

  • Ju, Jin-Sun;Shin, Yun-Hee;Kim, Eun-Yi
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.45 no.1
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    • pp.75-83
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    • 2008
  • We propose a welfare interface using multiple fecial features tracking, which can efficiently implement various mouse operations. The proposed system consist of five modules: face detection, eye detection, mouth detection, facial feature tracking, and mouse control. The facial region is first obtained using skin-color model and connected-component analysis(CCs). Thereafter the eye regions are localized using neutral network(NN)-based texture classifier that discriminates the facial region into eye class and non-eye class, and then mouth region is localized using edge detector. Once eye and mouth regions are localized they are continuously and correctly tracking by mean-shift algorithm and template matching, respectively. Based on the tracking results, mouse operations such as movement or click are implemented. To assess the validity of the proposed system, it was applied to the interface system for web browser and was tested on a group of 25 users. The results show that our system have the accuracy of 99% and process more than 21 frame/sec on PC for the $320{\times}240$ size input image, as such it can supply a user-friendly and convenient access to a computer in real-time operation.

Construction of web-based Database for Haliotis SNP (웹기반 전복류 (Haliotis) SNP 데이터베이스 구축)

  • Jeong, Ji-Eun;Lee, Jae-Bong;Kang, Se-Won;Baek, Moon-Ki;Han, Yeon-Soo;Choi, Tae-Jin;Kang, Jung-Ha;Lee, Yong-Seok
    • The Korean Journal of Malacology
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    • v.26 no.2
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    • pp.185-188
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    • 2010
  • The Web-based the genus Haliotis SNP database was constructed on the basis of Intel Server Platform ZSS130 dual Xeon 3.2 GHz cpu and Linux-based (Cent OS) operating system. Haliotis related sequences (2,830 nucleotide sequences, 9,102 EST sequences) were downloaded through NCBI taxonomy browser. In order to eliminate vector sequences, we conducted vector masking step using cross match software with vector sequence database. In addition, poly-A tails were removed using Trimmest software from EMBOSS package. The processed sequences were clustered and assembled by TGICL package (TIGR tools) equipped with CAP3 software. A web-based interface (Haliotis SNP Database, http://www.haliotis.or.kr) was developed to enable optimal use of the clustered assemblies. The Clustering Res. menu shows the contig sequences from the clustering, the alignment results and sequences from each cluster. And also we can compare any sequences with Haliotis related sequences in BLAST menu. The search menu is equipped with its own search engine so that it is possible to search all of the information in the database using the name of a gene, accession number and/or species name. Taken together, the Web-based SNP database for Haliotis will be valuable to develop SNPs of Haliotis in the future.