• Korean Journal of Plant Resources
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• v.16 no.2
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• pp.147-154
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• 2003

Agronomic characters of the 361 Korean adzuki beans(Vigna angularis (Willd.) Ohwi & Ohashi) collected in Korea were examined. Korean adzuki beans had predominantly up right growth habit types, followed by the intermediate types (15.3%) and the climbing types (6.9%). Round leaf type was the majority among the collections and followed by elliptical and xiphoid leaf type. Adzuki beans with yellow, light yellow and dark yellow flowers were 89.5％, 9.4％ and 1.1％, respectively. The 60.6% of the collections had red seeds and 26.9% had gray seeds. And collections with green seeds, white seeds mixed with gray spot, brown spots on red seeds, black spots on red seeds and dark gray spots on black seeds were also observed. Korean adzuki beans whose number of days from planting to flowering were 69∼75 days were the majority among 361 Korean adzuki beans. The number of days from flowering to majority were 41∼50 days and the number of days from planting to maturity were 111-120days. Adzuki beans having the stem length of 41-60 cm, the pod number per plant of 16-20 and the 100 seed weight of 8∼10 g were 50.2％, 40.2% and 30.5％ of collections, respectively.

• Korean journal of applied entomology
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• v.19 no.4 s.45
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• pp.221-227
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• 1980

A new disease of strawberry caused by Alternaria was found in Gim Hae, Gyougnam province, in July 1979. The symptoms of the disease occurred mainly on leaves as showing dark brown spots with circular to irregular, $5\~6mm$ in diameter. The pathogen was identified as Alternaria alternate (Fr.) Keissler which has not been described in Korea as a pathogen of strawberry disease. The common name of the disease was given as Gum un mum byung of strawberry. The conidia shape and size of this fungus varied considerably with culture media. The measurements of conidia and beak on corn meal agar were $20.1\times9.3{\mu}\;and\;4.0\mu$, respectively, which were shorter than those on strawberry leaf lesions in the field. Among 49 strawberry varieties tested, Morioca 16 and Robinson were highly susceptible to the disease by artificial inoculation and Catskill, Cyclone, Northwest, Merton princess, Juspa and Daehak I were moderately susceptible whereas 31 varieties were resistant. This fungus could also infect the leaf of Common garden sunflower, Double sunflower and Mexican sunflower among the 14 plants species tested.

• The Plant Pathology Journal
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• v.22 no.2
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• pp.119-124
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• 2006

Kudzu vine(Pueraria montana var. lobata) is an invasive climbing woody vine that envelops trees and shrubs, pressing physically and shutting out sunlight, which needs to be controlled. Kudzu vine pathogens were surveyed as a way to seek its biocontrol agents in 2002. Occurrence of a bacterial halo blight disease of kudzu vine was observed at several localities in Korea including Euiwang and Suwon in Gyeonggi Province, Daejon, and Gochang and Buan in Jeonbuk Province. Symptoms of brown to black spots with a surrounding yellowish halo appeared from June and lasted till the rainy season without much expansion, but accompanying often leaf blight and defoliation. Isolated bacteria were identified as Pseudomonas syringae pv. phaseolicola based on physiological and cultural characteristics, Biolog, fatty acid and 16S rDNA sequencing analyses. In artificial inoculation test, these bacteria produced the same halo spot symptoms on kudzu vine and bean plants. They also induced hypersensitive responses (HR) on tobacco, tomato, and chili pepper leaves. This is the first report of a bacterial disease of kudzu vine in Korea, and the bacterial pathogen can be used as a biocontrol agent against the pest plant.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.157-164
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• 2017

Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2, R. solani AG1-1, and Sclerotinia homoeocarpa. Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens, while ASC-35 was B. subtilis, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.419-426
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• 2006

This study investigated the ecology and growth of Monostroma nitidum Wittrock in both its natural habitat and the laboratory. The maximum length, width, and weight of M. nitidum in March were 9.0$\pm$4.7 cm, 9.6$\pm$3.6 cm, and 1.52$\pm$1.13 g, respectively. Yellowish-green or yellowish-brown reproductive thalli began to appear in January, and over 80% of the thalli matured by March. The male and female spores were ca. 6 $\mu$m long, and elongate and ovoid in shape. The spores had two flagella and one-eye spot, and tended to swim toward light. Maximum number of spores released from matured thalli was 236 cells/mL after 70 minutes at a light intensity of 100 $\mu$mol/m$^2$/s. The zygote diameter ranged from 3.4-6.0 $\mu$m (mean 4.2 $$m) and increased to 69.8 $\mu$m 14 weeks after culture. The mass release of zoospores was observed from thalli in the dark (3 to 12 days), after 30 min under dry conditions in the shade, at 25$^{\circ}C$, and a light intensity of 100 $\mu$mol/m$^2$/s. The maximum number of zoospores released was 109.8 cells/mL after 60 min of induction. M. nitidum fronds on the net increased to 6.8-7.2 cm in length, and 6.6-8.9 cm in width during the winter.

• Korean journal of applied entomology
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• v.23 no.1 s.58
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• pp.61-67
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• 1984

Conidial germination of Septoria glycines Hemmi, brown spot fungus of soybean, was studied by slide germination test. Poor conidial germination of S. glycines was observed on sterile distilled water, but potato dextrose agar(PDA) and distilled water floated with soybean leaf disc furnished a satisfactory medium for conidial germination. Exogenous supply of carbon source was essential for conidial germination, while phosphorous and potassium were not evident as that for carbon. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $5\times10^{-2}mol$. concentration of glucose. Germination was decreased with increment of conidial concentration and was almost completely suppressed in the density of 10,000 conidia per $mm^2$. It suggested existing a self-inhibitor(s). Non-washed conidia germinated more than washed conidia and this was obvious when the conidia density was over $2\times10^3$ conidia per $mm^2$ on the dry agar block.

• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.197-202
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• 2013

This study was aimed to find the storage potential of low temperature adapted shiitake mushroom under freezing temperature ($-3^{\circ}C$). Fresh shiitake mushroom was harvested at late Feb. and cooled to target temperature, $-3^{\circ}C$ in the cooling room for 24 hr. Cooled mushroom were then placed in plastic boxes, wrapped with plastic film ($30{\mu}m$), and stored at $-3^{\circ}C$ for 2 months. Weight loss of mushroom was ranged from 2.1 to 3.2%. Dry weight per unit fresh weight, however, was slightly increased because of moisture loss. Firmness of fruit body increased from $0.95kg/cm^{-2}$ (before storage) to $1.13kg/cm^{-2}$ (after 2 month storage). About half amount of starch was lost during 2 month storage. The amount of total and reducing sugars remained relatively constant. After storage, freshness of mushroom was recovered by thawing treatment. When recovered mushroom were packaged with styrofoam tray and PVC wrapping, and exposed to ambient and $10^{\circ}C$, respectively, brown spot on the gill of fruit body was found and slight decay symptom was also found at ambient temperature only but not at $10^{\circ}C$. Results indicated that low temperature adapted shiitake mushroom has a storage potential under freezing temperature ($-3^{\circ}C$). Freezing storage technology of fresh shiitake mushroom will contribute the increase of storability up to 2 months.

• Korean journal of applied entomology
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• v.18 no.3 s.40
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• pp.119-126
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• 1979

From the sample seeds of rice cultivar Palkoeng with the brown spot symptom, Helminthosporium oryzae was detected in $86\~92\%$ of hulls including empty glumes, lemma, palea, and rachilla in five to seven days of incubation. When the seeds were dehulled, the percentage detection decreased to $74\~83\%$ of pericarps. The fungus sporulated on whole surface of the seed and was detected within about ten cell layers deep of endosperm but not detected in embryo. Fusarium moniliforme was found in embryo as well as pericarp and endosperm. The frequency of F. roseum, Trichoconis padwickii, Curvularia spp., Atternaria tenuis, Cladosporium cladosporoides and Phoma sp. was less than three percent of hulls and pericarps. In seven days of incubation, $60\~87\%$ of the germinating seeds showed the symptom of H. oryzae while $13\~40\%$ were apparently healthy. This fungus in hilum of infected rice transmitted through pericarp to plumule shoot and radicle of the germinating seed.

• Asian Journal of Turfgrass Science
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• v.12 no.1
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• pp.23-30
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• 1998

174 isolates of soil microorganisms were isolated from E-golf club from Apr.1997 through Oct. 1997. And 27 strains of them were selected through the inhihition test of mycelial growth. In the same period, soil-borne diesease pathogens, "Rhizoctonia", causing Large patch, Brown patch, Spring dead spot, and Yellow patch were isolated from the diseased areas in E-golf and S-golf club. The antagonistic activity of the strains against the pathogens was tested to select the excel-lent antagonists. In contact with the fungicides, the survivability of the antagonists was tested to assess the compatibility of the antagonists with the pesticides. The results were as follows: 1.Suppression of Rhizoctonia by Antagonists. Antagonistic activity of 27 strains against the pathogens was: tested in vitro. In the result, 3 isolates(B-7, B-15, B-41) of bacteria and 2 isolates(F-5, F-47) of fungi were superior to the rest. 2.Compatibility of the antagonists: with the fungicides: With 13 kinds of pesticides widely using Golf Club, Compatibility of 5 antagonists: were finally tested to select the strains: that mostly survived in contact with pesticides. In the results:, two of five strains: were selected : one strain was bacteria B-15, the other strain was fungi F-47. 24h after the mixing with pesticides:, these two strains were shown to survive at 90% level and these were identified as Bacillus and Trichoderma, respectively. And the most compatible pesticides: with the antagonists were shown to Polytoxin-D thirarn(s:urvivability 99.4%) and Validamycin-A (survivability 98.6%). Keywords:Antagonist, Large Patch, Trichoderma, Compatibility, Fungicide.Fungicide.

• Proceedings of the Ginseng society Conference
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• 1978.09a
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• pp.43-49
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• 1978

This study was devised to observe the cytotoxic activity of extracts of Panax ginseng root against some cancer cells and to purify the crude extract. Three kinds of cancer cells(leukemic cells L5178Y, HeLa cells and Sarcoma 180 cells) and mouse embryo cells (as normal cells) were used for this study. The ginseng roots were extracted with petroleum ether in soxhlet apparatus, and the crude extracts were purified by the silicic acid column chromatography and thin-layer chromatography methods. The results obtained are summarized as follows; 1. Eight to ten mg of the petroleum ether extract (crude extract) were obtained from 1 g of Panax ginseng root, and its activities per mg were about 1,000 units. 2. Doubling time of the L5178Y cells was increased to two fold by 24 hours incubation in culture medium containing about one ${\mu}g$ of extract per ml, and eight and ten folds higher concentration of ginseng extract were required for the Sarcoma 180 cells and HeLa cells, respectively, than for the leukemic cells(L5178Y) to inhibit the cellular growth to the same degree. 3. When the L5178Y cells were exposed to medium containing various concentration of the extract for 24 hours before initiation of the soft agar cloning procedure, about $99\%$ of the L5178Y cells were killed at concentration of 8 units per ml. 4. The growth rate of mouse embryo cell (as normal cell) was not affected by the culture with media containing various amounts (1.45 to 30.0 ${\mu}g/ml$) of the extract. 5. The crude extract could be purified about four times by silicic acid column chromatography using several solvent systems, and one spot of active compound could be obtained on the thin-layer chromatogram. 6. In the Swiss mice inoculated with Sarcoma 180 cells, a survival time of the experimental group (injection group of active compound) was extended more. 1.5 to 2.0 times than the control group's(no injection group).