• 한국해양바이오학회지
• /
• 제2권3호
• /
• pp.187-191
• /
• 2007

The radical scavenging activity of methanol extracts of seaweeds and their active compounds, alginic acid, fucoidan and phloroglucinol, were investigated under in vitro. Among methanol extracts of seaweeds (sea mustard, sea tangle, seaweed papulosa, fusiforme, sea lettuce, purple laver and chlorella), seaweed papulosa and sea tangle showed strong scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical (${\cdot}OH$). In addition, under in vitro, the scavenging activities on DPPH radical of alginic acid and fucoidan, which are active compounds of brown algae, and phloroglucinol, the active compound from Ecklonia species, were evaluated and compared. Fucoidan and phloroglucinol showed strong DPPH scavenging effect, in particular, phloroglucinol had strongest activity among the active compounds. On the other hand, alginic acid did not exert DPPH scavenging activity. From the present study, we could confirm the antioxidative activity of seaweeds and its active compounds.

• 한국해양바이오학회지
• /
• 제1권3호
• /
• pp.170-177
• /
• 2006

본 연구는 제주산 해조류의 생리활성을 대량검색하기 위해 HTS 기법을 이용한 실험과정의 제어 및 관리를 위한 자동화 시스템을 구축하고자 DPPH 라디칼을 이용하여 HTS를 이용한 자동화 시스템을 구축하고 제주산 해조류의 DPPH 라디칼 소거활성을 대량 검색하고자 하였다. 그 결과 HTS를 위한 효율적인 검색방법을 개발하였고 DPPH 라디칼 소거활성이 높은 해조류를 검색하게 되었다. 녹조류의 경우 납작파래 (20G6)의 $20^{\circ}C$ 추출물(1 mg/ml)에서 약 60%이 DPPH 라디칼 소거활성을 보였을 뿐 나머지 해조류에서는 40% 미만이 낮은 활성만을 보였다. 갈조류의 경우 꽈배기 모자반 (20B17), (70B17), 참그물바탕말 (70B1), 큰잎모자반 (70B16)과 감태 (70B26)에서 DPPH 라디칼 소거활성이 우수하였다. 홍조류 중에서는 왜떨기나무붉은실 (20R24)에서 가장 우수한 DPPH 라디칼 소거활성을 나타내었고 갈래잎 (20R17), 굵은참우뭇가사리 (20R18), 부채분홍잎 (20R23) 또한 높은 소거활성을 보였다. 따라서 우수한 효과를 나타내었던 해조류를 대상으로 용매분획 및 활성물질의 분리, 정제를 통해 해조류 유래의 기능성 신소재를 개발한다면, 해조류의 고부가가치화 및 HTS기술의 보편화에 큰 영향을 미칠 것으로 판단된다.

• 한국축산시설환경학회지
• /
• 제6권3호
• /
• pp.141-151
• /
• 2000

Over-grown seaweeds in Donghae(east sea of Korea peninsula) may impedes ocean environment, however, they can be a good nutrient resources for poultry feeds if they are utilized properly. In this study, seaweeds powder were tested for laying hens\` ration to investigate the effects on egg production rate, egg quality and calcium phosphorus excretion. One hundred 65wks-old brown layers were fed for 5 weeks alotted with seaweeds powder addition to experimental diet by 0(control), 0.5, 1.0 and 2.0%, respectively, and obtained following results; 1. Seaweeds proved a mid-protein low-energy feed resources with planty of K, Na, Ca, Mg, Sr and Fe. 2. Seaweeds addition by 0.5% and 1.0% improved egg production rate and egg-mass output markedly(p<0.05) than control. Seaweeds addition did not alter cholesterol level of yolk and yolk index, however egg shell thickness showed increasing trend by increasing seaweeds addition level. 3. Protein absorption and digestibility in seaweed addition treatments were significantly higher(p<0.05) than control group and protein contents of excreta in 0.5% and 1.0% treatments were reduced(p<0.05), which suggests effective protein metabolism for egg production. Increasing seaweeds addition reduces Ca and P contents in rectum and excreta, suggesting Ca and P utilization improvement in laying hens and lessening soil pollution. 4. In conclusion, seaweeds addition in layers' diet by 1.0% level improves egg-mass production and might be egg quality by increasing metabolism of protein, calcium and phosphorus.

• 대한지역사회영양학회지
• /
• 제15권3호
• /
• pp.361-368
• /
• 2010

This study was carried out to investigate preference and purchase factors of seaweed in some regional residents of Korea. Subjects were residents (n = 1,218) whose residential area was divided into inland and coastal region and the survey was done during December 2007. Especially, purchase factors of seaweeds was conducted only in married females (n = 353). The subjects are composed of 46.5% male and 53.5% female. Regional distribution of subjects was found to be 16~17%, with highest ratio in the age bracket of 20~29 years old. Proportion of students, at 29.8%, was the highest ranking occupation of the subjects. Preference score of seaweeds by region was highest for laver followed by brown seaweed and sea tangle. In terms of preference by gender, female subjects displayed higher preference score for green laver (p < 0.01), seaweed fusiforme, brown seaweed, sea tangle (p < 0.001) than the male subjects. Considerations being made when purchasing seaweeds for each region were in the order of freshness, taste and nutrition. In comparing the inland and coastal region, scores of freshness, convenience, design and color for the coastal region was higher, displaying significant difference.

• ALGAE
• /
• 제18권1호
• /
• pp.71-81
• /
• 2003

• Ocean Science Journal
• /
• 제43권1호
• /
• pp.31-37
• /
• 2008

As a part of an ongoing search for antioxidants from marine sources, antioxidant activities of 24 kinds of seaweeds (4 green algae, 8 brown algae, and 12 red algae) were investigated. The seaweeds were extracted by acetone/dichloromethane and methanol, respectively. The antioxidant properties of both extracts were evaluated using four different activity tests, including degree of occurrence of intracellular reactive oxygen species (ROS), NO, lipid peroxidation, and GSH (glutathione) in mouse macrophage Raw 264.7 cells. The levels of intracellular reactive oxygen species (ROS) and GSH were measured using 2',7'-dichlorofluorescin diacetate (DCFDA) and monobromobimane as fluorescence probe, respectively. Moreover, the generation of NO and lipid peroxidation products were determined by each method based on the Griess reaction and TBARS assay. Solvent extracts from seaweeds such as Scytosiphon lomentaria, Prionitis cornea, Laruencia okamurae, Callophyllis japonica, Sargassum horneri, Dictyopteris divaricata, Lomentaria catenata, Corallina confuse, Ishige okamurae, and Ahnfeltiopsis flabelliformi exhibited high antioxidant activities in cellular oxidizing systems.

• 한국가금학회:학술대회논문집
• /
• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
• /
• pp.38-52
• /
• 2003

Seaweeds are large algae that grow in a saltwater or marine environment. There are over 400 species of seaweeds in the bay of Korea. Most seaweeds are divided into three groups according to their color : the green seaweeds(division Chlorophyta), the brown(Phanphyta) and the red(Rhodophyta), Seaweeds have been harvested for use as food, fertilizer, animal feed and medicines for thousands years. In the past decade, there has been increasing interest in using seaweeds for animal feedstuffs and feed supplements as their health qualities are becoming better known. In this review some perspectives on nutritional values and the role of functionality ingredients of various seaweeds will be also discussed. Ecklonia cava kjellman(EC), a perennial seaweed, is one of the main components of the marine forests along the East and South coasts of Korea. We conducted three experiments to evaluate the nutritional values of EC and the crude lectin extracted residues from EC(LEEC) and to investigate their dietary effects on productivity and immunocompetence in poultry. The crude protein content of EC was relatively low(about 10 ％) and its amino acids were poorly available. But, the results of our study suggested that EC can be used into broiler feed up to 3 ％ without any adverse effect, if its nutritional values are well evaluated prior to use. Similar results of dietary LECC up to 1.0 ％ level on egg production and egg quality have been obtained in layers. The concentrations of serum TNF-${\alpha}$ after Salmonella challenge were rapidly increased in accordance with the level of LEEC. These results indicated that LEEC can be used as a feed additives for enhancing of immunocompetence in poultry.

• 한국수산과학회지
• /
• 제45권6호
• /
• pp.686-693
• /
• 2012

The seaweed community and stomach contents of the dominant herbivorous sea urchins Anthocidaris crassispina and Hemicentrotus pulcherrimus were examined from November 2008 to October 2009, in coastal areas of Tongyeong, Korea. Thirty-five seaweeds including two green, nine brown, and 24 red algae, were found over the study period. Seventeen seaweeds (two green, two brown, and 13 red) and two invertebrates were found in the stomachs of A. crassispina. In H. pulcherrimus, stomach contents were two green, one brown, 11 red seaweeds, and two invertebrates. The shell diameter of A. crassispina and H. pulcherrimus ranged from 22.3 to 62.3 mm and 15.3 to 40.1 mm, respectively. Total body weights ranged from 48.5 to 86.7 g for A. crassispina and from 7.7 to 25.9 g for H. pulcherrimus. The total weight of stomach contents were 5.1 to 25.8 g in A. crassispina and 1.7 to 11.8 g in H. pulcherrimus. The range of gonad weight was 3.6 to 17.0 g in A. crassispina and 0.8 to 4.0 g in H. pulcherrimus. The gonad index (GI) for A. crassispina peaked in July and reached a minimum in December, whereas the GI for H. pulcherrimus was highest in February, and lowest in May.

• 한국수산과학회지
• /
• 제15권4호
• /
• pp.263-270
• /
• 1982

해중의 영양학적인 기초자료를 얻기 위하여 multi-enzyme system을 이용한 체외소화율과 아미노산분석을 기초로 한 단백질수를 측정하였다. 김 (P. tenera)의 체외소화율은 $78.5\sim82.2$로서 산지와 건조조건에 따라 약간의 차이를 보였으며, 잎파래(E. linza)나 다른 갈조류 (미역 U. pinnatifida, 톳 H. fusiforme, 모자반 S. fuuellum)에 비하여 높았고 효소활성 저해물질 (trypsin inhibitor)의 함량은 갈조류에서 높았다. 잎과래는 채외소화율이 김보다 낮음에도 불구하고 효소활성저해물질이 가장 낮은 특이한 결과론 보였다. 전체적으로 해조류의 체의소화을이 다른 연구자들의 생체실험에 의한 소화율 (in vivo digestility)보다 높은 결과를 보인 것은multi-enzyme system을 이용한 체외소화율 측정 방법을 해조류의 정확한 소화율 측정에 적용하기에는 문제성이 있는 것으로 밝혀졌다. 일륜 김에 대한 microwave cooking의 영향은 가열시간이 15분 경과하여도 현저한 소화율 증가는 볼 수 없었으며, 효소활성 저해물질함량은 서서히 감소하는 경향을 보였다. 또한 한국식으로 구운김의 체외소화율은 microwave로 15분 가열한 시료와 비슷하였다. 아미노산 분석 결과를 이용한 단백계수(Factor method)는 김의 경우 6.52로 계산되었고, 잎파래는 6.00, 미역 6.11, 모자반 5.85, 톳은 5.83이었으며, Kjeldahl전소분석결과를 이용한 단백계수(Kjeldahl Method)는 김 6.29, 잎파래 5.83, 미역 5.40, 모자반 5.45, 톳 5.49로 나타나, 종래의 조단백계수(6.25)보다 낮은 결과를 보였다. 해조추의 비단백태질소의 정확한 규명이 없는 상태에서, 해조의 단백질함량 측정에는 아미노산 분석결과를 이용한 새로운 단백계수(Factor Method)를 사용함이 바람직한 것으로 생각되었다.

• 한국환경보건학회지
• /
• 제26권4호
• /
• pp.75-81
• /
• 2000

A matrix modification was studied for the determination of trace manganese in several seaweeds by electrothermal atomic absorption spectrophotometry(ETAAS). The type and quantity of modifiers were investigated to realize the efficient modification. Palladium was chosen as a single modifier. By the addition of palladium(5$\mu\textrm{g}$/$m\ell$) to 2ng/$m\ell$ manganese solution, the temperatures were raised from 1,00$0^{\circ}C$ to 1,20$0^{\circ}C$ for the charring and from 2,10$0^{\circ}C$ to 2,20$0^{\circ}C$ for the atomization as well as the sensitivity and reproducibility were improved. With such optimum conditions, the trace amount of manganese in several seaweeds(laver, tangle and brown seaweed) could be determined by a calibration curve method, and good recoveries of more than 965 were also obtained in the samples in which a given amount of manganese was spiked. The detection limit of this method was about 0.048ng/$m\ell$.