Ocean Science Journal

The Korean Society of Oceanography (한국해양학회)
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Antioxidant Efficacy of Extracts from a Variety of Seaweeds in a Cellular System

  • Kim, You-Ah (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Kong, Chang-Suk (Research Institute of Marine Science and Technology, Korea Maritime University) ;
  • Um, Young-Ran (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lee, Jung-Im (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
  • Published : 2008.03.30
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Abstract

As a part of an ongoing search for antioxidants from marine sources, antioxidant activities of 24 kinds of seaweeds (4 green algae, 8 brown algae, and 12 red algae) were investigated. The seaweeds were extracted by acetone/dichloromethane and methanol, respectively. The antioxidant properties of both extracts were evaluated using four different activity tests, including degree of occurrence of intracellular reactive oxygen species (ROS), NO, lipid peroxidation, and GSH (glutathione) in mouse macrophage Raw 264.7 cells. The levels of intracellular reactive oxygen species (ROS) and GSH were measured using 2',7'-dichlorofluorescin diacetate (DCFDA) and monobromobimane as fluorescence probe, respectively. Moreover, the generation of NO and lipid peroxidation products were determined by each method based on the Griess reaction and TBARS assay. Solvent extracts from seaweeds such as Scytosiphon lomentaria, Prionitis cornea, Laruencia okamurae, Callophyllis japonica, Sargassum horneri, Dictyopteris divaricata, Lomentaria catenata, Corallina confuse, Ishige okamurae, and Ahnfeltiopsis flabelliformi exhibited high antioxidant activities in cellular oxidizing systems.

Keywords

References

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