• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.4
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• pp.199-204
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• 1977

The patterns of fatty acid composition of lipid extracted from eight species of edible marine benthic algae; i. e. two species of green, five of brown and one of red algae, were investigated quantitatively by using gas liquid chromatography. The total lipid contents in average of the algae were $1.51\%$ in the green algae, $2.81\%$ in brown algae and $1.02\%$ in red algae respectively. Upon analyzing fatty acid composition, green algae contained the highest proportion of $C_{16:0}$ acid and comparatively higher content of $C_{18:1},\;C_{18:2}\;and\;C_{18:3}$ acids than the other algae. In brown angae, $C_{16:0}$ acid content was highest, but not more than that of green algae, and $C_{14:0},\;C_{18:1}$ acids were higher than other $C_{16:1},\;C_{18:2}\;and\;C_{18:3}$ acids while the content of $C_{18:0}$ acid was very low. Red algae showed low content of $C_{14:0},\;C_{18:1},\;C_{18:2}\;and\;C_{18:3}$ acids, but the content of $C_{16:0}$ acid was high as a major component. In regard to the composing patterns of carbon number of fatty acid of algae; i. e. $C_{14},\;C_{15},\;C_{16},\;C_{18}\;and\;C_{22}$ and $C_{22}$ acids, the green algae contained $C_{16}$ and $C_{18}$ acids, the brown algae $C_{16},\;C_{18},\;and\;C_{22}$ and $C_{22}$ acids, and the red algae $C_{15},\;C_{16}$, and $C_{18}$ acids as the major component.

• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.54-59
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• 2020

The inconsistent methods of harvest, identification, and preparation of brown algae samples make it difficult to compare the results of certain measurements across different studies, such as total phenolic contents (TPC), total flavonoid contents (TFC), and total antioxidant capacities (TAC). Therefore, this study aims to systematically measure and compare TPC, TFC, and TAC in 10 types of standardized brown algae extracts provided by the Bio-Resource Information System of Korea. Among the ten brown algae extracts, Ishige foliacea exhibited the highest levels of TPC and TAC, followed by Sargassum nigrifolium. TAC was measured by the following four assays: ABTS, DPPH, superoxide radical scavenging activities, and ferric reducing antioxidant power. TAC was found to be positively correlated with TPC (p<0.05) but not TFC, indicating that Ishige foliacea and Sargassum nigrifolium have potential use as marine antioxidative food material.

• Applied Chemistry for Engineering
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• v.20 no.5
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• pp.517-521
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• 2009

In this study, the productivity of bio-ethanol obtained from various brown-algae raw materials was examined. Brown-algae polysaccharide, consisting of alginate and laminaran, is usable for the effective production of bio-ethanol if it is hydrolyzed to monomer unit. The objective of this study is the production of bio-ethanol from brown-algae using a heat-treatment and acid-treatment. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 and Pachysolen tannophilus KCTC 7937 strains. Laminaran japonica was higher than Sagassum fulvellum and Hizikia fusiformis, Laminaran japonica optimum pre-treatment is used to derive the ethanol production of Saccharomyces cerevisiae KCCM1129 and Pachysolen tannophilus KCTC 7937 respectively 9.16 g/L, 9.80 g/L. The maximum output of Sargassum fulvellum and Hizikia fusiformis was very low as 0.22 g/L.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.465-474
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• 2014

This study was performed to investigate the inhibitory effects of brown algae extracts on histamine production in mackerel muscle. First, antimicrobial activities of brown algae extracts against Morganella morganii were investigated using a disk diffusion method. An ethanol extract of Ecklonia cava (ECEE) exhibited strong antimicrobial activity. The minimum inhibitory concentration (MIC) of ECEE was 2 mg/ml. Furthermore, the brown algae extracts were examined for their ability to inhibit crude histidine decarboxylase (HDC) of M. morganii. The ethanol extract of Eisenia bicyclis (EBEE) and ECEE exhibited significant inhibitory activities (19.82% and 33.79%, respectively) at a concentration of 1 mg/ml. To obtain the phlorotannin dieckol, ECEE and EBEE were subjected to liquid-liquid extraction, silica gel column chromatography, and HPLC. Dieckol exhibited substantial inhibitory activity with an $IC_{50}$ value of 0.61 mg/ml, and exhibited competitive inhibition. These extracts were also tested on mackerel muscle. The viable cell counts and histamine production in mackerel muscle inoculated with M. morganii treated with ${\geq}2.5 $ MIC of ECEE (weight basis) were highly inhibited compared with the untreated sample. Furthermore, treatment of crude HD-Cinoculated mackerel muscle with 0.5% ECEE and 0.5% EBEE (weight basis), which exhibited excellent inhibitory activities against crude HDC, reduced the overall histamine production by 46.29% and 56.89%, respectively, compared with the untreated sample. Thus, these inhibitory effects of ECEE and EBEE should be helpful in enhancing the safety of mackerel by suppressing histamine production in this fish species.

• Preventive Nutrition and Food Science
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• v.20 no.3
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• pp.153-161
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• 2015

Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that have important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Marine plants are on the rise for their potential to provide natural products that exhibit remarkable health benefits. In this context, brown algae species have been of much interest in the pharmaceutical field with reported instances of isolation of bioactive compounds against tumor growth and MMP activity. In this study, eight different brown algae species were harvested, and their extracts were compared in regard to their anti-MMP effects. According to gelatin zymography results, Ecklonia cava, Ecklonia bicyclis, and Ishige okamurae showed higher inhibitory effects than the other samples on MMP-2 and -9 activity at the concentrations of 10, 50, and $100{\mu}g/mL$. However, only I. okamurae was able to regulate the MMP activity through the expression of MMP and tissue inhibitor of MMP observed by mRNA levels. Overall, brown algae species showed to be good sources for anti-MMP agents, while I. okamurae needs to be further studied for its potential to yield pharmaceutical molecules that can regulate MMP-activity through cellular pathways as well as enzymatic inhibition.

• Journal of Plant Biology
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• v.26 no.4
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• pp.181-190
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• 1983

Seasonal occurrence of benthic algae and changes of subtidal vegetation were studied for their species composition, diversity and biomass during 1982 and 1983 at several selected sites at Juckdo Island (38$^{\circ}$12'N, 128$^{\circ}$32'E), eastern coast of Korea. Three large brown algae which played a role in change of algal vegetation through their great biomass were investigated with regard to their seasonal growth. Large brown algae such as Undaria pinnatifida, Costaria costata, Laminaria japonica, Agarum cribrosum, Sargassum confusum and S. hornerii constitute the major portion of vegetation in this area throughout the year. Algal vegetation in spring time is characterized by dominance of species U. pinnatifida and C. costata, whereas the summer vegetation by S. confusum and S. hornerii. In autumn large brown algae are shedded and only small algae, such as Chondrus ocellatus and Grateloupia filicina, remain. The vegetation in winter is dominated by the growth of U. pinnatifida and C. costata. Monthly changes in mean length and weight of randomly collected U. pinnatifida, C. costata and S. confusum are as follows; U. pinnatifida occurs from December to June and shows their maximum growth during March (120 cm in length, 201 g/individual in wet weight), its maximum growth rate is 1.4 cm/day, 3.3 g/day in this month. The growth season of C. costata is very similar to U.pinnatifida, but their average maximum length(110 cm) and weight (106 g/ind.) are lower than U. pinnatifida. The greatest growth rate is during March (1.8 cm/day, 2.0g/day). S. confusum is present throughout the year and reaches the maximum growth (102 cm, 63g/ind.) in July. Maximum growth rate (1.5 cm/day, 1.2 g/day) occurs also during this month. U. pinnatifida and C. costata show different months of maximum growth evidently during the two year. This seems to be caused by a considerable damage to the local vegetation followed by heavy storm in February 1983.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.5
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• pp.621-628
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• 1993

Total lipid contents from 10 species of marine algae(2 green, 5 brown, and 3 red algae) collected from different places in Korea were examined and their fatty acid compositions were compared among species and habitats. Total lipid(TL) was prominent in green laver(about 7.3~10.1%) of the green algae, in sea mustard and seaweed fusiforme(about 3.1~4.8%) of the brown algae and purple laver(about 4.9~6.4%) of the red algae. Sea mustard and seaweed fusiforme collected at Chungmu contained a relatively high level of TL than that at Yosu and Kijang. The TL content of purple laver showed the highest portion in that collected at Nakdong. Green algae comprised the majority of n-3 fatty acids(29.0~66.3%), which mainly consisted of 16 : 4(n-3) (or 16 : 3 (n-3)), 18 : 3(n-3) and 18 : 4(n-3). Brown algae accounted for a low level of n-3 fatty acids(17.9~36.5%) mainly 18 : 4(n-3), 18 : 3(n-3) and 20 : 5(n-3), whereas the brown algae contained a significant level of n-6 fatty acids(7.23~26.5%) such as 20 : 4(n-6) and 18 : 2(n-6). In the case of red algae, the n-3 fatty acids consisted mostly of 20 : 5(n-3) which scored 53% of polyenoic acids in purple laver collected at Nakdong. The proportion of n-3 fatty acids in algae belonging to the same species was higher in algae of high TL contents. Consequently, TL and n-3 fatty acid levels from the seaweeds studied in this paper were different from their habitats.

• Food Science and Preservation
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• v.21 no.2
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• pp.294-300
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• 2014

This study was performed to determine the effect of brown algae on the egg quality of laying hens fed a non-supplemented diet (Control) and a diet supplemented with 10% Undaria pinnatifida (A), Laminaria religiosa (B), and Hijikia fusiforme (C), respectively. Isa Brown (28 weeks old) were raised for 8 weeks. The darkness, redness, and blueness of the egg yolks increased with the addition of brown algae, expecially Undaria pinnatifida (A). The fucoxanthin contents were observed to be only 21.3-34.8 mg% level in the brown algae but they were nearly traced in the egg yolks. Among the analyzed minerals, the Ca, Fe, K contents increased to 391.2, 6.3, and 450.5 mg%, respectively, but the amount of P was reduced to 110.5 mg%. The total mineral contents were higher in the egg yolk with Hijikia fusiforme (C) (1,155.1 mg%) than with any other supplement. The brown algae diet reduced the saturated fatty acid level from 38.3 to 32.1% but increased the unsaturated fatty acid level from 61.5 to 69.6% owing to the rise of the level of polyunsaturated fatty acids.

• Analytical Science and Technology
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• v.9 no.4
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• pp.373-381
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• 1996

Algal biomass was used in our study in order to remove some metals. After packing of 40~60 mesh algae powder into column for use of metal adsorbent, the metal solution of 1mL/min of flow rate was eluted to adsorb in algae. More amount of Cu(II) or Zn(II) ion in green algae, Ulva pertusa Kjellman than in brown algae, Sargassum horneri (Turner) C. Agarch were adsorbed and Cu(II) ion was more adsorbed in both algae than Zn(II) ion. Recovery of metal from algae is showing higher in acidic or neutral than in alkalic conditions. Cu(II) ion is recovered relatively higher than Zn(II) ion in our system.

• 한국해양학회지
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• v.10 no.1
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• pp.25-32
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• 1975

Continued to the previous paper, the present investigation deals with stronitium-90 activities and calcium contents among 54 samples of edible marine algae collected along the coast of Korea during September, 1973 and April, 1974. The calcium contents are variable 2.0-17.8%, and 6.38% on an average. Most of the members investigated contain 2-4% in green and red algae, and 8-10% in brown algae. Strontium-90 activities are 0.32-0.37 pCi/l in sea-water, and 0.80-28.66 pCi$\^$90/Sr/g Ca in edible seaweeds. Among the algal phyla, they are 12.49 in green, 3.34 in brown, and 9.39 pCi$\^$90/Sr/g Ca in red algae, while they are 6.25 pCi$\^$90/Sr/g Ca on an average. In a single species collected at the same season, the highest activities appear mostly from the eastern coast, and from the western and southern coasts, in turn. The green algae, Capsosiphon- Enteromorpha complex show about 3 times higher activities, 28.66 pCi$\^$90/Sr /g Ca compared with the other members showing rather higher activities, and would be an indicator plant of strontium-90 activities of the marine algae along the coast of Korea.