• Title/Summary/Keyword: Brilliant green

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Diagnosis of Salmonella dubin in Korean Native Calves using PCR and Nucleotide Sequences of rfb5 Gene (송아지에 감염된 Salmonella dublin의 PCR 진단과 rfbS 항원단백 유전자의 염기서열분석)

  • 김철민;이영준;박명규;최경성;김민석
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.464-469
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    • 2000
  • An epizootic of calf diarrhea occurred in a Korean native cattle farm located in Chonbuk province. The calves that had either bloody or watery diarrhea were 1 to 30 days old. Some of these animals died during the acute course of the disease. Five calves with predominant clinical signs were examined in more detail. Hematological and serum chemical findings were suggestive of dehydration and nutritional insufficiency. Fecal material from the calve was cultured on/in brilliant green agar (BGA), xylose-lysine deoxycholate (XLD) medium, MacConkey agar, eosin methylene blue (EMB) agar and triple sugar iorn (TSI) A bacteria was isolated. which was subsequently identificed as belonging to Salmonella spp. To differentiate Salmoenlla serotype, rfbs gene of S. dublin was amli- find (720 bp) by multiplex (PCR). The rfbS gene sequences of S, dublin ficld isolate(SDC-1) was com- pared with that off S. dublin(S-37) S, dublin(Ahn et al, 1996), S enteritidis(Ahn et al 1996)and S. typhi (Generbak accession No M29682). The identities of nucleotide sequences were 100%. 99.6%, 99.6%, 97.5% respectively.

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Facile Synthesis of MoS2-C60 Nanocomposites and Their Application to Catalytic Reduction and Photocatalytic Degradation

  • Li, Jiulong;Ko, Weon Bae
    • Elastomers and Composites
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    • v.51 no.4
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    • pp.286-300
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    • 2016
  • $MoS_2$ precursors were synthesized by reacting thioacetamide ($C_2H_5NS$) with sodium molybdate dihydrate ($Na_2MoO_4{\cdot}2H_2O$) in aqueous HCl solution. $MoS_2$ nanoparticles were prepared from dried $MoS_2$ precursors by calcination in an electric furnace at $700^{\circ}C$ for 2 h under an inert argon atmosphere. $MoS_2-C_{60}$ nanocomposites were obtained by heating $MoS_2$ nanoparticles and fullerene ($C_{60}$) together in an electric furnace at $700^{\circ}C$ for 2 h. Their morphological and the structural properties were characterized by powder X-ray diffraction and scanning electron microscopy. The $MoS_2$ nanoparticles and $MoS_2-C_{60}$ nanocomposites were used as catalysts in the reductions of 2-, 3-, and 4-nitrophenol in the presence of sodium borohydride. The photocatalytic activities of the $MoS_2$ nanoparticles and $MoS_2-C_{60}$ nanocomposites were evaluated in the degradation of organic dyes (brilliant green, methylene blue, methyl orange, and rhodamine B) under ultraviolet light (254 nm).

Floral Image Make-up - Centered on Georgia O'Keeffe's Paintings - (Floral Image Make-up에 관한 연구 - 조지아 오키프(Georgia O'Keeffe)의 작품을 중심으로 -)

  • Kim, Hyo-Sook;Kang, In-Ae
    • Journal of the Korean Home Economics Association
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    • v.43 no.11 s.213
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    • pp.97-107
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    • 2005
  • Ed- the file appears to be corrupted, and in many sections (these are highlighted) I cannot read it. I presume it has been copied from another format, maybe Hangul 2004. The purpose of this study was to determine a method of make-up image extraction from specific paintings and also to create cyber make-up models according to the images. For this study, Georgia O'Keeffee's floral paintings were analyzed and their colors were compiled to make color palettes. This study attempted to approach floral image make-up which applies specific paintings through the digital mode in the manner of computer graphics. The results of this study were as follows: First, we found romantic images, including feminine, lovely and soft images by Y, GY and RP group colors, in 'Two Calla Lillies on Pink'. Second, we found modem images, including urban, up-to-date and cool images by G, GY and B group colors, in 'Blue and Green Music'. Third, we found sexy images, including brilliant, tempting and daring by R, B and G group colors, in 'Music-Pink and Blue'. To summarize, the images of the paintings were similar to those of the make-up models.

Comparison of Gold Biosensor Combined with Light Microscope Imaging System with ELISA for Detecting Salmonella in Chicken after Exposure to Simulated Chilling Condition

  • Mi-Kyung Park
    • Journal of Microbiology and Biotechnology
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    • v.33 no.2
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    • pp.228-234
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    • 2023
  • In this study, the performance of a gold biosensor combined with light microscope imaging system (GB-LMIS) was comparatively evaluated against enzyme-linked immunosorbent assay (ELISA) for detecting Salmonella under simulated chilling condition. The optimum concentration of antiSalmonella polyclonal antibodies (pAbs) was determined to be 12.5 and 100 ㎍/ml for ELISA and GBLMIS, respectively. GB-LMIS exhibited a sufficient and competitive specificity toward three tested Salmonella among only. To mimic a real-world situation, chicken was inoculated with Salmonella cocktail and stored under chilling condition for 48 h. The overall growth of Salmonella under chilling condition was significantly lower than that under non-exposure to the chilling condition (p < 0.05). No significant differences in bacterial growth were observed between brain heart infusion and brilliant green broth during the enrichment period (p > 0.05). Finally, both GB-LMIS and ELISA were employed to detect Salmonella at every 2-h interval. GB-LMIS detected Salmonella with a competitive specificity by the direct observation of bacteria on the sensor using a charge-coupled device camera within a detection time of ~2.5 h. GB-LMIS is a feasible, novel, and rapid method for detecting Salmonella in poultry facilities.

A Comparative Study of Plant Patterns Found in the Textiles of Goryeo and its Neighbor Countries (고려와 주변국 직물에 표현된 식물무늬 비교 연구)

  • Yeom, Ha-Ryoung;Cho, Hyo-Sook
    • Journal of the Korean Society of Costume
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    • v.59 no.9
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    • pp.71-86
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    • 2009
  • This study examines plant patterns expressed on the fabrics during the Goryeo Dynasty and many countries in the Chinese continent and analyzes their symbolism and formativeness. This study deals with clothes which is one of the most fundamental aspects in a culture and plant patterns on them; it examines the overall formativeness of plant patterns in East Asia from the 10th century to the 14th century by studying plant patterns in the Goryeo Dynasty and the contemporaneous dynasties in the Chinese continent - Five Dynasties, Song Dynasty, Western Xia Dynasty, Liao Dynasty, Jin Dynasty and Yuan Dynasty. The plant patterns of each country were categorized by the type of plant. The composition and expression of plant patterns were examined and statistically analyzed. Small flowers were found in Goryeo fabrics twice more than other types that could be clearly identified, and peony was the most popular flower used in Chinese fabrics. In terms of composition of plant patterns, both Goryeo and Chinese fabrics had plant only patterns more frequently than the patterns mixed with animals or jewels. Regarding expression methods of plant patterns, the most common one in Goryeo fabrics was the petal-type, while the branch-type is the most common one in Chinese fabrics. The plant patterns of the Goryeo Dynasty show beauty of simplicity with minimalism and simplification while expressed with brilliant sold threads on dark background, such as purple or light green, so the overall feeling of fabrics was simple yet nobel.

Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil

  • Park, Mi-Kyung;Chin, Bryan A.
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2051-2059
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    • 2016
  • To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was $3,219{\pm}755Hz$, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.

식품내의 미생물 분리를 위한 dryfilm 방법의 평가연구

  • 하상도
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.178-184
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    • 1996
  • Dryfilm method by using 3M Petrifilm$^{TM}$ has been examined to replace conventional agar method for isolation of microorganisms from foods. The objectives of the present study were to evaluate suitability of dryfilm method as a microbial isolation method and to determine the effect of antimicrobial agent on dryfilm for isolation of microorganisms from foods. Five different foods, milk, ground beef, fishery surimi, Takju and wheat flour were used to isolate the natural microflora in foods and the inoculated Escheri chia coli. Standard method agar (SMA, Difco) and Petrifilm$^{TM}$ aerobic count (PAC, 3M) were used to isolate total microorganisms from foods. Violet red bile agar (VRBA), brilliant green lactose bile (BGLB) broth and Petrifilm$^{TM}$ coliform count (PCC, 3M) were used to isolate coliforms from foods. E. coli broth (EC broth) and Petrifilm$^{TM}$ E. coli count (PEC, 3M) were used to isolate E. coli from foods. Acidified potato dextrose agar (APDA) and Petrifilm$^{TM}$ yeast & mold count (PYMC, 3M) were used to isolate yeasts and molds from foods. Total aerobic plate counts isolated from five different foods by SMA and PAC (3M) were riot significantly different each other at P<0.05 level and were highly correlated each other ($\geq$0.96). Mugwort extract as an antimicrobial agent did not affect microbial enumeratiion of Dryfilm. Significantly higher number of coliform colonies were formed on VRBA than PCC (3M) from ground beef, but they were not significantly different in coliform colonies from milk samples. PCC (3M) and BGLB were not significantly different for enumeration of coliforms in milk and beef samples. Significantly higher number of E. coli were isolated by EC broth than PEC from ground beef, but these were not significontly different for enumeration of E. coli from milk. Yeast and mold counts isolated from Takju and wheat flour by APDA and PYMC (3M) were not significantly different at P<0.05 level. These data indicate that dryfilm method by using 3M Petrifilm$^{TM}$ can be successively used as an alternative to conventional agar method for enumeration of microorganisms in various foods.

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Natural Dyeing Using Tea Extract II - The Effect of Dyeing Condition on Dyeing Characteristics of Coffee Extract Dyed Silk Farbic-

  • Yoo, Hyun-Geun;Kim, Sin-Hee
    • Journal of Fashion Business
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    • v.10 no.3
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    • pp.78-86
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    • 2006
  • Coffee extract dyeing on silk fabric using various mordanting agents at different mordanting condition was studied in our previous research. Coffee extract dyeing can exert beautiful colors and have several advantages over the conventional natural dyeing. Since most of natural dyes are expensive for mass production, strongly season and source dependent, and hard to store, and therefore, coffee waste was used as natural dye to create beautiful colors in this study. Coffee waste is inexpensive and easy to store, and provide a comparatively regular quality regardless of season and source. The effects of dyeing condition such as dyeing temperature, dyeing time, and liquor ratio on dyeing characteristics of silk fabrics were investigated. Coffee extract dyed silk showed brilliant colors, and the colors were different with the kind of mordant. Cu, Sn, and Al mordanted silks showed golden yellow with subtle change of hue and value, while Fe mordanted silk showed a brownish green color upon coffee extract dyeing. As the dyeing temperature increased, the dyeability generally increased in all mordanted silk fabrics except Fe mordanted silk until $45^{\circ}C$. The dyeing temperature did not affect the dyeability of Fe mordanted silk fabric. As the liquor ratio increased, the dyeability showed a gradual increase in all mordanted silk except Cu mordanted silk. Cu mordanted silk fabric showed an abrupt increase in dyeability at liquor ratio1:150. The dyeing time effect can be separated into 2 stages. During the initial half an hour, the dyeability did not change significantly, and then, increased after 45 min. dyeing time duration. A further increase in dyeing time after 45 min. did not affect the dyeing efficiency in all mordanted silk fabrics except the Cu mordanted.

The ionization energy and lattice parameters of Co- and Ce-doped cubic zirconia (YSZ) single crystal (Co와 Ce를 첨가한 큐빅지르코니아(YSZ) 단결정의 이온화에너지 및 격자상수)

  • Seok, Jeong-Won
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.20 no.4
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    • pp.159-163
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    • 2010
  • Co-(0.5 mol%) and Ce-(0~0.3 mol%) doped cubic zirconia ($ZrO_2:Y_2O_3$=64:36 mol%) single crystals grown by a skull melting method were heat-treated in $N_2$ at $1200^{\circ}C$ for 3 hrs. The brown-colored as-grown single crystals were changed into either green or blue color after the heat treatment. Before and after the heat treatment, the YSZ (yttriastabilized zirconia) single crystals were cut for wafer form (${\phi}7mm{\times}t2mm$) and round brilliant cut ($\phi$ 12 mm). The optical and structural properties were examined by UV-VIS spectrophotometer and X-ray diffraction. Absorption by $Ce^{3+}(^2F_{5/2},\;_{7/2}(4f){\rightarrow}^2T_g(5d^1)),\;Co^{2+}(^4A_2(^4F){\rightarrow}^4T_1(^4F)$ or $^4T_1(^4P))$ and $Co^{3+}$, change of ionization energy and lattice parameter were confirmed.

A Study on Wadding Dresses for Women in the Latter Period of Chosun (조선후기 여자 혼례복에 관한 연구)

  • 전혜숙;김숙경
    • The Research Journal of the Costume Culture
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    • v.10 no.2
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    • pp.160-177
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    • 2002
  • Wedding ceremony is a most basic and significant rite of religion. Clothing fur the ceremony is also assumed religious in essence. Thus this study focuses on ideas and religious qualities implied in wedding dresses for women in the latter period of Chosen. Among those wedding dresses for women in the public class, in this study, Yeom-Eui(염의), Won-Sam(원삼) in green and Hwal-Ot(활옷) are discussed. Yeom-Eui seemed preferred by only some of the nobel class who still considered manners and customs as very important. The rest people often wore a brilliant Hwal-Ot rather than Yeom-Eui under influences of a loosened social position system and sumptuous moods. Since a wedding is the reflection of social condition and at the same time a religious rite, the above mentioned difference in wedding dresses between the class of scholars obsessed with Confucianism and the rest public seems attributable to differences in values and religious views between the two groups. Of course, Hwal-Ot was also transmitted from the Chinese nation of Tang, so it complied with a contemporary flunkeyism about Chinese culture. Won-Sam and Hwal-Ot were designed with patterns representing the very significance of wedding and those wishing worldly blessings more children and more sons, longevity and wealth and prosperity. The fact that wishes of more children and more sons were more often implied by patterns of wedding dress in the latter Chosen indicates the legitimate oldest son-oriented patriarchical family system at that time influenced to such contemporary dresses. Meanwhile, those patterns used for Won-Sam and Hawl-Ot were influenced mainly by Confucianism, but sometimes based on Buddhism and Taoism. It suggests that the Chosun dynasty emphasized Confucian manners and customs to restore previous values which had been about to be collapsed since wars with the Chinese Ching and Japan, but nevertheless in the public class, Buddhism and Taoism were more deeply prevailed. This was supported by patterns and colors shown in wedding clothing.

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