Juhyoung Sung;Sungyoon Cho;Da-Eun Jung;Jongwon Kim;Jeonghwan Park;Kiwon Kwon;Young Myoung Ko
Journal of Internet Computing and Services
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v.24
no.1
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pp.61-69
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2023
Recently, as the fishery resources are depleted, expectations for productivity improvement by 'rearing fishery' in land farms are greatly rising. In the case of land farms, unlike ocean environments, it is easy to control and manage environmental and breeding factors, and has the advantage of being able to adjust production according to the production plan. On the other hand, unlike in the natural environment, there is a disadvantage in that operation costs may significantly increase due to the artificial management for fish growth. Therefore, profit maximization can be pursued by efficiently operating the farm in accordance with the planned target shipment. In order to operate such an efficient farm and nurture fish, an accurate growth prediction model according to the target fish species is absolutely required. Most of the growth prediction models are mainly numerical results based on statistical analysis using farm data. In this paper, we present a growth prediction model from a stochastic point of view to overcome the difficulties in securing data and the difficulty in providing quantitative expected values for inaccuracies that existing growth prediction models from a statistical point of view may have. For a stochastic approach, modeling is performed by introducing a Gaussian process regression method based on water temperature, which is the most important factor in positive growth. From the corresponding results, it is expected that it will be able to provide reference values for more efficient farm operation by simultaneously providing the average value of the predicted growth value at a specific point in time and the confidence interval for that value.
Variation in ploidy level of regenerated plants from rice anthers and effective diploidization methods of haploid plants were studied to obtain basic information in rice breeding through anther culture. In a total of 574 plants derived from anther culture using 14F$_1$ hybrids as materials, there were 49.7% haploids, 48.6% diploids and 1.7% polyploids, respectively. The frequency of haploids in Japonica/Indica crosses was 60.6%, and that of Japonica/Japonica crosses was 43.0% in average. Inclusion of 2.4-D or NAA as phytohormone may increase the frequency of haploids, but kinetin may increase the frequency of diploids. The rate of auto-diploidization by tiller separation of haploid plants showed 8.2% in average. The rate of diploidization by leaf-sheath injection of colchicine showed 18.8% in average. Morphological characters of haploids plants showed that 64.6% in culm length, 63.4% in panicle length, 68% in flag leaf length, and 74.4% in flag leaf width compared to diploid plants. These apparent morphological differences will contribute to identify the ploidy of plants derived from rice anther culture.
To obtain basic information on the breeding of early maturing, short plant height rapeseed varieties, the following 7 varieties, Isuzu, Miyuki, Norin 25, Rang, Yongdang, Cresus and Tower were used in diallel crosses in 1979. Maturing date, plant height and grain weight per plant for the parents, $F_1$'s and $F_2$'s of the 7 x 7 partial diallel crosses were measured in 1981 for analysis of their genetic behavior. The results obtained are summarized as follows: 1. The days to maturing of $F_1$'s showed complete dominance for early maturing, and both additive and dominance genetic variances were significant. Number of effective factors in $F_1$'s was 3, but in $F_2$'s was 1. The degree of dominance in $F_1$'s was partial, while in $F_2$'s was complete. Both broad and narrow sense herita-bilities in $F_1$'s was high, while in $F_2$'s was low. 2. Yield per plant in $F_2$'s was controlled by additive component of genetic variance only, but $F_1$'s was different. The degree of dominance in $F_1$'s was complete, while in $F_2$'s was partial. The direction of dominance showed almost complete dominance over high yield and three effective factors was estimated. Yield per plant was controlled by recessive genes. 3. The plant height was controlled by both dominance and additive variance. Dominance was directed toward tall plant height. Number of effective factors was 2, and broad and narrow sense heritability were high in the plant height.
Chun Mae Dong;Mi-Nan Lee;Jae Koo Noh;Jin Woo Park;Young-Ok Kim;Eun-Mi Kim
Journal of Life Science
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v.33
no.8
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pp.623-631
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2023
This study was conducted to develop microsatellite markers in Parapristipoma trilineatum using next-generation sequencing. A total of 402,244,934 reads were generated on the Illumina Hiseq X Ten System, yielding 60,738,985,034 bp of sequences. The de novo assembly resulted in 1,320,995 contigs. A total of 952,326 contigs (0.016%) including 151 microsatellite loci were derived from the 1,320,995 contigs longer than 640 bp. A total of 34 primer sets were designed from the 151 microsatellite loci. As a result, 15 microsatellite loci were chosen and used for assuming population genetic parameters in the wild and farmed populations. The mean number of effective alleles was 12, ranging from 6 to 25. The observed heterozygosity (HO) and the expected heterozygosity (HE) ranged between 0.530 and 0.873, with an average of 0.750, and from 0.647 to 0.895, with an average of 0.793, respectively. According to these results, the developed set of 15 microsatellite markers is expected to be useful for the analysis of genetic characteristics in the population of P. trilineatum in Korea. There are requirements now for further genetic information, fishery resource management, breeding guidelines, support with the selection of breeds and studies on the effects of release, all of which will improve species conservation, and through future research, we aim to offer genetic foundational data with that goal.
Eun Jee Lee;Yoon A Kim;Mi Sun Lee;Ju Hyeok Kim;Young Kyu Choi;Jung Sung Kim;Chang Seob Sin;Yi Lee
Korean Journal of Plant Resources
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v.36
no.4
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pp.290-298
/
2023
Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.
The evaluation of soybean germplasm has mainly been carried out by morphological characters at Genetic Resources Division, Rural Development Administration (RDA). However, this information has been limited serving a diverse information for user and effectively managing the soybean germplasm. To resolve this problem, soybean collection conserved at RDA gene bank was profiled using nine soybean SSR (Simple Sequence Repeat) markers. Soybean SSR allele was confirmed using genescan and genotyper softwares of automatic sequencer for accurate genotyping of each accession and continuous accumulation of data. SSR profiling of soybean germplasm has been carried out from 2,855 (Satt458) to 4,368 (Satt197) accessions by locus. The number of allele revealed 267 with an average of 29.6 in total accession, and varied from a low of 21 (Satt532 and Satt141) to a high of 58 (Sat_074). Although the number of accessions of wild soybean is less than that of soybean landraces, Korean wild soybean is more variable than other soybean landraces populations in total number of alleles. However, Korean soybean landraces were more variable than Korean wild soybeans in 5 loci. In the allele frequency, wild soybean accessions showed an even distribution in all alleles and higher distribution in low ladder than in high ladder. Also, Korean soybean landraces revealed a high condensed frequency in Satt286 (202 bp, 232 bp), Chinese soybean landraces in Satt197 (171 bp) and Satt458 (173 bp), and Japanese soybean landraces in Sat_074 (244 bp) and Satt458 (170 bp). These SSR profile information will be provided as indications of redundancies or omissions of accessions and can aid in managing soybean collection held at RDA gene bank. The information on diversity analysis could help to enlarge the genetic diversity of materials in breeding program, and could be used to develop a core collection of soybean germplasm.
A series of experiments were conducted at Crop Experiment Station from 1968 to 1971 to obtain basic information on effects of selection on yield of wheat varieties. Heritability estimates, correlation and path coefficients computed for yield and yield related characters from fixed variety groups-Korean, U.S., and Japan; early and late-$F_2$ and $F_3$ generations were studied. The same estimates for fixed variety groups grown under different fertilizer levels and years were also studied. The results were summarized as follow: 1. Three variety groups classified by their origins as Korea, United States and Japan showed high heritability estimates for heading date, plant height and spike length. The heritability estimates for grain number per spike and 1, 000 grain weight were moderate and those for the number of spikes per plant and grain yield were low. Very low estimates of heritability were obtained for grain number per spike and yield of variety group from the Unites States. 2. High genotypic correlation coefficients between 1, 000 grain weight and yield were obtained for all variety groups originated from Korea, United States and Japan and early variety group, except late variety group. The genotypic correlation coefficients between grain number per spike and yield were also high for all variety groups except variety group originated from the United States. 3. The direct effects of 1, 000 grain weight in terms of path-coefficients to yield were high for all variety groups except late variety group. 4. High genotypic correlation coefficients were obtained between 1, 000 grain weight and yield in $F_2$ from. two crosses. The same degree of genotypic correlation coefficients were obtained between grain number per spike and yield, although slight differences in its magnitude were found depending upon the cross combination. 5. The analysis of path-coefficients in $F_2$ shows that the direct effects of yield components to yield were negligible. 6. The characters that showed high genotypic correlation coefficients with yield in $F_3$ were 1, 000 gnain weight and grain number per spike. These characters showed also high direct effects to yield. 7. No great variations of heritability estimates for heading date, plant height and spike length were obtained for either fertilizer responsive or non responsive variety group due to fertilizer levels applied. 8. Heritability estimates of 1, 000 grain weight in fertilizer responsive group and yield in fertilizer nonresponsive group were high as level of fertilizer increased. 9. Heritability estimates for grain number per spike and 1, 000 grain weight of fertilizer non-responsive-group were higher than those of fertilizer. responsive group. 10. Genotypic corretation coefficients between yield and 1, 000 grain weight in fertilizer responsive group were getting lower as the level of fertilizer increased and those in fertilizer non-responsive group were vice versa. 11. Genotypic correlation coefficient between yield and spike number per plant in fertilizer responsive group was high. However, the genotypic correlation coefficient between yield and spike number per plant in fertilizer non-responsive group was low. 12. The direct effects of 1, 000 grain weight to yield were higher than other yield components either in fertilizer responsive or non-responsive group regardless of levels of fertilizer applied. The spike number per plant, however, was high only when high level of fertilizers were applied to fitilizer responsive group. 13. Slight variations of heritability estimates for heading dates, plant height, spike length, grain number per spike and 1, 000 grain weight were obtained between years. However, the spike number per plant with low heritabilility showed great variation between years. 14. The character that showed high genotypic correlation coefficients with yield in two years was 1, 000 grain weight, and this character was also high in direct effect to yield in terms of path-coefficients. 15. From the above experimental results, it might be concluded that 1, 000 grain weight would be one of the most important characters to increase the effects of selection for yield in wheat breeding in Korea.
Seo, Dong Won;Sultana, Hasina;Choi, Nu Ri;Kim, Yeon Su;Jin, Shil;Heo, Kang Nyeong;Jin, Seon Deok;Lee, Jun Heon
Korean Journal of Poultry Science
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v.42
no.1
/
pp.1-8
/
2015
Recently, duck meat consumption has been rapidly increased because consumers recognized duck meat for healthy food. In relation to this, Korean duck industry need to develop Korean native duck (KND) breed for both conservation perspective and self-sufficient of the breeding stocks. In this study, 24 microsatellite (MS) markers were investigated for classification of KND and commercial duck (CD) breeds in the Korean market. Using these MS markers, the calculated number of alleles (K), expected heterozygosity (He) values and polymorphic information contents (PIC) were 1~16, 0~0.865 and 0~0.841, respectively. Also, the expected probability of identical values in random individuals (PI), random sib ($PI_{sib}$) and random half-sib ($PI_{half-sib}$) were estimated as $1.64{\times}10^{-16}$, $2.60{\times}10^{-7}$ and $1.30{\times}10^{-12}$, respectively. The results indicated that the expected probabilities of identity powers were enough for the individual identification. However, KND and CD breeds were not fully discriminated well using the 24 MS markers, which may CD and KND has shared same origin or crossbred. Therefore, further studies will be ultimately needed for developing a genetically pure line of KND breed even though the DNA markers used. Finally, these results will provide useful information for individual traceability system in ducks.
This study was conducted to investigate relationships among cow daily milk yield, calf body weight at birth and 3 month of age and calf preweaning daily gain. Cow daily milk yield(DMILK) of 90 days after postpartum measured by weigh-suckle-weight method, calf birth weight(BW), calf body weight at 3 month of age(WT3), and calf preweaning daily gain from birth to 3 month of age(PDG) were collected from June to November on 2002 and April to November on 2003. All traits studied were considered as cow traits and analysed by WOMBAT packages to obtain A-I REML(average information restricted maximum likelihood) estimates. Heritability estimate of daily milk yield uncorrected for cow-calf separation time(DMILK) was 0.39, which was not differ from 0.36 of heritability estimate corrected on the basis of 24 hour cow-calf separation time(CMILK). Heritability estimates for maternal genetic effects of BW, WT3 and PDG were respectively 0.28, 0.17 and 0.18. Genetic correlations between DMILK and maternal effect of BW, WT3 and PDG were respectively 0.59, 0.79 and 0.68. High genetic correlation coefficients of DMILK with WT3 and PDG indicate that selections for maternal breeding value for WT3 or PDG may be effective to enhance the cow’s milk production ability in Hanwoo.
Developing and breeding improved legume-based food resources require collecting useful genetic traits with heritability even though requiring some time-consuming, costly, and labor intensive. We attempted to infer heritability of nine genetic traits-days to flowering, days to maturity, period from flowering to maturity, the number of seeds per pod, 100-seeds weight, and four contents such as crude protein, crude oil, crude fiber, and dietary fiber-using 455 homologous chloroplast gene sets of six species of legumes. Correlation analysis between genetic trait differences and phylogenetic distance of homologous gene sets revealed that days to flowering, the number of seeds per pod, and crude oil content were influenced by genetic factors rather than environmental factors by 62.86%, 69.45%, 57.14% of correlated genes (P-value ≤ 0.05) and days to maturity showed intermediate genetic effects by 62.42% (P-value ≤ 0.1). The period from flowering to maturity and 100-seeds weight showed different results compared to those of some previous studies, which may be attributed to highly complicated internal (epistatic or additive gene effects) and external effects (cultural environment and human behaviors). Despite being slightly unexpected, our results and method can widely contribute to analyze heritability by including genetic information on mitochondria, nuclear genome, and single nucleotide polymorphisms.
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