• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.360-367
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• 2011

Beta-carotene producing transformants were produced in the background of 'Nagdongbyeo', a Japonica rice cultivar. Introgression of the carotenoid locus in the transformant, PAC4-2 into the elite cultivar 'Ilpumbyeo' was started. To initiate a backcrossing program, we surveyed 220 SSR markers and found that 38% of them were polymorphic between 'Ilpumbyeo' as a recurrent parent and the PAC4-2 as a recipient parent. The selection strategy comprising foreground and background selection was employed. First, foreground selection was practiced in $BC_1$, $BC_2$, and $BC_3$ generations using the transgene specific PCR-based marker in addition to visual scoring of the seed color. Marker-based background selection combined with phenotypic selection was employed from $BC_3F_2$ to $BC_3F_4$ generations. Blast search indicated that the transgene PAC4-2 was located between SSR markers, RM6 and RM482. 240 $BC_3F_3$ and 63 $BC_3F_4$ lines were evaluated for four agronomic traits including days to heading. Most of the lines were similar to Ilpumbyeo in agronomic traits evaluated. The percentage of PAC4-2 genome ranged from 4% to 21% with a mean of 12.5%, which was higher than the expected for an unselected $BC_3$ backcross population. This could be explained by the fact that two genes for beta-carotene and the stripe virus resistance were targeted in this study. We selected 10 representative $BC_3F_5$ lines from 63 $BC_3F_4$ lines based on agronomic traits and carotenoids content. The selection strategy would be appropriate for the introgression of beta-carotene gene in a breeding program.

• Korean Journal of Breeding Science
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• v.43 no.2
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• pp.133-138
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• 2011

The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1618-1618
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• 2001

Predicting quality traits using near infrared (NIR) spectroscopy on whole grain samples has gained wide acceptance as a non-destructive, rapid and cost effective technique. Barley breeding programs throughout southern Australia currently use this technology as a tool for selecting malting quality lines. For the past 3 years whole grain barley calibrations have been developed at VIDA to predict malting quality traits in the early generation selections of the breeding program. More recently calibrations for whole grain malt have been developed and introduced to aid in selecting malted samples at the mid-generation stage for more complex malting quality traits. Using the same population set, barley and malt calibrations were developed to predict hot water extracts (EBC and IoB), diastatic power, free $\alpha$-amino nitrogen, soluble protein, wort $\beta$-glucan and $\beta$-glucanase. The correlation coefficients between NIR predicted values and laboratory methods for malt were all highly significant ($R^2$ > 0.84), whereas the correlation coefficients for the barley calibrations were lower ($R^2$ > 0.57) but still significant. The magnitude of the error in predicting hot water extract, diastatic power and wort $\beta$-glucan using whole grain malt was reduced by 50% when compared with predicting the same trait using whole grain barley. This can be explained by the complex nature of attempting to develop calibrations on whole grain barley utilizing malt data. During malting, the composition of barley is modified by the action of enzymes throughout the steeping and germination stages and by heating during the kilning stage. Predicting malting quality on whole grain malt is a more reliable alternative to predicting whole grain barley, although there is the added expense of micro-malting the samples. The ability to apply barley and malt calibrations to different generations is an advantage to a barley breeding program that requires thousands of samples to be assessed each year.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1483-1503
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• 2011

To advance the effectiveness of the current Hanwoo improvement system, we developed a general simulation that compared a series of breeding schemes under realistic user circumstances. We call this system the Integrated General Model (IGM) and it allows users to control the breeding schemes and selection methods by manipulating the input parameters. The Current Hanwoo Performance and Progeny Test (CHPPT) scheme was simulated with a Modified Hanwoo Performance and Progeny Test (MHPPT) scheme using a Hanwoo Breeding Farm cow population of the Livestock Improvement Main Center (LOMC) of the National Agricultural Cooperatives Federation (NACF). To compare the two schemes, a new method, the Simple Hanwoo Performance Test (SHPT), which uses ultrasound technology for measuring the carcass traits of live animals, was developed. These three models, including the CHPPT, incorporated three types of selection criteria: phenotype (PH), true breeding value (TBV), and estimated breeding value (EBV). The simulation was scheduled to mimic an actual Hanwoo breeding program; thus, the simulation was run to include the years 1983-2020 for each breeding method and was replicated 10 times. The parameters for simulation were derived from the literature. Approximately 642,000 animals were simulated per replication for the CHPPT scheme; 129,000 animals were simulated for the MHPPT scheme and 112,000 animals for the SHPT scheme. Throughout the 38-year simulation, all estimated parameters of each simulated population, regardless of population size, showed results similar to the input parameters. The deviations between input and output values for the parameters in the large populations were statistically acceptable. In this study, we integrated three simulated models, including the CHPPT, in an attempt to achieve the greatest genetic gains within major economic traits including body weight at 12 months of age (BW12), body weight at 24 months of age (BW24), average daily gain from 6 to 12 months (ADG), carcass weight (CWT), carcass longissimus muscle area (CLMA), carcass marbling score (CMS), ultrasound scanned longissimus muscle area (ULMA), and ultrasound scanned marbling score (UMS).

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.720-734
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• 2023

In Korea, Korea Proven Bulls (KPN) program has been well-developed. Breeding and evaluation of cows are also an essential factor to increase earnings and genetic gain. This study aimed to evaluate the accuracy of cow breeding value by using three methods (pedigree index [PI], pedigree-based best linear unbiased prediction [PBLUP], and genomic-BLUP [GBLUP]). The reference population (n = 16,971) was used to estimate breeding values for 481 females as a test population. The accuracy of GBLUP was 0.63, 0.66, 0.62 and 0.63 for carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS), respectively. As for the PBLUP method, accuracy of prediction was 0.43 for CWT, 0.45 for EMA, 0.43 for MS, and 0.44 for BFT. Accuracy of PI method was the lowest (0.28 to 0.29 for carcass traits). The increase by approximate 20% in accuracy of GBLUP method than other methods could be because genomic information may explain Mendelian sampling error that pedigree information cannot detect. Bias can cause reducing accuracy of estimated breeding value (EBV) for selected animals. Regression coefficient between true breeding value (TBV) and GBLUP EBV, PBLUP EBV, and PI EBV were 0.78, 0.625, and 0.35, respectively for CWT. This showed that genomic EBV (GEBV) is less biased than PBLUP and PI EBV in this study. In addition, number of effective chromosome segments (M_e) statistic that indicates the independent loci is one of the important factors affecting the accuracy of BLUP. The correlation between M_e and the accuracy of GBLUP is related to the genetic relationship between reference and test population. The correlations between M_e and accuracy were -0.74 in CWT, -0.75 in EMA, -0.73 in MS, and -0.75 in BF, which were strongly negative. These results proved that the estimation of genetic ability using genomic data is the most effective, and the smaller the M_e, the higher the accuracy of EBV.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.144.2-145
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• 2003

This study was performed to investigate the incidence of Hosta uirus X (HVX), a Potexvirus, from cultivated hosta ornamental plants in Korea and to ascertain seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants. Infection rate of HVX in cultivated hostas was 25.6 ％ (11 out of 43 collected samples contained HVX) based on Western blot and RT-PCR detection methods. Most of HVX-infected hostas showed visible systemic leaf symptoms (mosaic, mottle, curling, stunting or combinations). Variability of HVX was confirmed by sequences of coat protein gene of individual isolates from different hostas. HVX was seed-transmitted on Hosta 'Blue Cadet'. The virus was detected from seeds, and sprouts and seedlings from the virus-contaminated seed sources. Over 7.5 ％ of seeds were HVX-contaminated surveyed in this study, Our data suggest that HVX can be transmitted by seed source, and indexing of the virus should be done for breeding program of Hosta.

• Mycobiology
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• v.32 no.1
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• pp.11-16
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• 2004

Winter mushroom, Flammulina velutipes, needs low temperature during its cultivation. To save on farm costs, especially during summer, a strain adaptable to a higher or elevated-temperature must be developed. At the start of breeding program, parental strains which could endure high temperature were obtained. Seuenty four dikaryotic strains were collected and divided into four groups according to the nature of temperature. They also had different fruiting temperature. Finally we selected three brown strains ASI 4048, 4057 and 4072, and collected their spores. These selected strains can germinate even at a high temperature of $32^{\circ}C$, which were dramatically higher than the other strains. Based on these results, the new white strain adapted to mid-temperature by backcross mating was developed. Molecular markers were applied to select white fruitbody producing strains without cultivation. They showed a specific band which co-segregated with brown fruitbody forming strains in $BC_1F_1$ progenies. Selected white strains were tested under several elevated temperature conditions.

• Korean Journal of Veterinary Service
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• v.27 no.3
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• pp.273-280
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• 2004

The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1. Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448 bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: $95^{\circ}C$ for 30 sec, $45^{\circ}C$ for 30 sec, and $72^{\circ}C$ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were $6.6\%,\;53.3\%$ and breeding farms $65\%,\;65\%\;and\;38.7\%$, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average $21\%$ in domestic farms, $56.2\%$ in breeding farms, and $29.9\%$ in AI center.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1019-1024
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• 1999

Fifth one Nili-Ravi dairy buffaloes were studied in North-west Frontier Province of Pakistan. Rectal examination of reproductive organs was carried out on days 14 and 21 and then fortnightly. Milk samples were collected and analyzed for progesterone levels (MPL). Feed samples were collected fortnightly and analyzed. The buffaloes calving during the normal breeding season (NBS, August to January) (p<0.01) postpartum estrus interval of 55.95 days versus 91.15 days in those calving during the low breeding season (LBS, February to July). MPL in the LBS remained lower than the NBS (p<0.01). Shortest postpartum ovulation interval was noted during autumn(August to October), followed by winter (November to January), summer (May to July) and spring (February to April). The incidence of silent ovulations was during LBS than NBS (70.6% versus 29.4%). In autumn there was minimum intake of crude protein (CPI) and maximum intake of metabolizable energy (MEI, p<0.01). Calcium intake was higher in NBS than LBS calving buffaloes (p<0.01). Phosphorus, copper and magnesium intake was lower (p<0.05) and zinc intake was higher (p<0.01) in autumn, It was (p<0.05) and zinc intake was higher (p<0.01) in autumn, It was concluded that onset of breeding season was associated with increasing MEI and decreasing CPI and minerals intake.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.143-143
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• 2017

Wheat is one of the most important crops in production and consumption. Despite increasing of importance, the self-sufficiency of wheat is less than 2% in Korea. To improve yield potential and broaden the genetic pool of common wheat in Korea, introduction of alien germplasms into the Korean wheat breeding program is suggested. For effective utilization of the germplasm, we introduced total 1,195 germplasms from the world, which were provided by National Plant Germplasm System (NPGS, USDA) and evaluated the yield, field performances and agronomic traits for 8 years. Among 55 countries, germplasms from Canada, Ethiopia, Mexico and United States accounts for 78%, especially germplasms collected from United States accounts for 50%. Yield comparison of germplasms and collected region analysis indicate that the high yielding germplasms are collected from countries or states of particular range of latitude. The combination analysis of the yield and agronomic traits and the geographical information of collected region will be utilized for improving Korean wheat breeding programs.