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Diagnosis of porcine reproductive and respiratory syndrome (PRRS) and its serological survey using the reverse transcription and polymerase chain reaction (RT-PCR) and ELISA  

Chu Keum-Suk (Jangsu-Branch, Jeonbuk Development & Livestock Research Institute)
Han Keu-Sam (Jangsu-Branch, Jeonbuk Development & Livestock Research Institute)
Han Jae-Cheol (Iksan-Branch, Jeonbuk Development & Livestock Research Institute)
Song Hee-Jong (Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.27, no.3, 2004 , pp. 273-280 More about this Journal
Abstract
The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1. Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448 bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: $95^{\circ}C$ for 30 sec, $45^{\circ}C$ for 30 sec, and $72^{\circ}C$ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were $6.6\%,\;53.3\%$ and breeding farms $65\%,\;65\%\;and\;38.7\%$, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average $21\%$ in domestic farms, $56.2\%$ in breeding farms, and $29.9\%$ in AI center.
Keywords
PRRS; RT-PCR; ELISA;
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