• Title/Summary/Keyword: Breeding Program

Search Result 420, Processing Time 0.03 seconds

Reaction of Near-Isogenic Lines with Resistance to Bacterial Blight to Korean and Japanese isolates of Xanthomonas oryzae pv. oryzae (한국 및 일본 균주에 대한 벼흰잎마름병 저항성 근동질 유전자 계통의 반응)

  • Kim, Bo-Ra;Yang, Cheol-Woo;Jin, Xuan-Ji;Han, Jin-Soo;Lee, Eun-Jeong;Choi, Jae-Eul
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.52 no.1
    • /
    • pp.89-99
    • /
    • 2007
  • To develop durable and stable breeding strategies far the control of rice bacterial blight (BB) disease, the near-isogenic lines (NILs) with diverse resistance genes to BB isolates were evaluated in plant at three different growth stages using four Korean ($K_1,\;K_2,\;K_3,\;K_{3a}$) and three Japanese isolates (I, II, III). The resistance of the NILs to seven BB isolates tended to increase with plant aging. At the seedling stage, the NILs with single resistance genes were mostly resistant to $K_1$ race but they showed partial or no resistance to other isolates. A NILs (IRBB5) possessing xa5 had full resistance to the four Korean isolates, illustrating that it is a useful source to give enhancement to Korean breeding program. At the maximum tillering stage, resistance of NILs to $K_2,\;K_3$, I and II isolates considerably increased while resistance to $K_1,\;K_{3a}$ and III were similar to those of seedling stage. NILs with resistance gene of Xa4, xa5 and Xa7 proved to be the most stable to BB isolates at the maximum tillering stage. At the heading stage, most resistance genes of NILs were effective against BB isolates, and xa5 showed the consistent resistance to all the BB isolates including $K_{3a}$ and III isolates, demonstrating that resistance genes of Xa4, xa5 and Xa7 can be used either alone or combined to enhance resistance to BB disease in Korea.

EST Profiling for Seed-hair Characteristic and Development of EST-SSR and SNP Markers in Carrot (당근 종모 형질 관련 EST profiling과 이를 이용한 EST-SSR 및 SNP 마커 개발)

  • Oh, Gyu-Dong;Hwang, Eun-Mi;Shim, Eun-Jo;Jeon, Sang-Jin;Park, Young-Doo
    • Horticultural Science & Technology
    • /
    • v.28 no.6
    • /
    • pp.1025-1038
    • /
    • 2010
  • Carrot ($Daucus$ $carota$ L. var. $sativa$) is one of the most widely used crops in the world. Moreover it is an important crop because of its high content of ${\beta}$-carotene, well-known as the precursor of vitamin A carotenoid. However, seed-hair which is generated in epidermal cell of seeds inhibits absorption and germination. For that reason, carrot seeds are commercialized after mechanical hair removal process. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-ATR615 OP 666-13line and hairy seed CT-ATR615 OP-CK1-9 line were constructed and expression patterns related to generation of seed-hair were analyzed by comparison of EST sequences. Differential EST sequence results between two lines were classified into FunCat functional categories based on the results of BlastX search. Higher expression quantities belonging to metabolic category were shown on short-hair seed line than hairy-seed one. Differential expression quantities between those two lines in the protein folding and stabilization, subcellular localization categories were supposed to contribute variously on the generation of seed-hair. We confirmed 50 and 59 SSR sites, and 2 SNP sites by analyzing EST sequences in two lines; thereafter, we designed SNP and SSR primer sets from these EST sequence information as a molecular marker. These markers are thought to be used in research of molecular markers for classification of carrot family and related to various traits, as well as seed-hair characteristic.

Relationship Between Ultrasonic and Carcass Measures for Meat Qualities in Hanwoo Steers (한우 거세우에 있어서 생체단층조사기법에 의한 육질판정과 도축 육질 측정치와의 관계)

  • Kim, H. C.;Lee, D. H.;Choi, S. B.;Jeon, G. J.
    • Journal of Animal Science and Technology
    • /
    • v.45 no.2
    • /
    • pp.183-190
    • /
    • 2003
  • Data from live animal real time ultrasound (RTU) measures from 127 Hanwoo steers were used to assess nutritional physiology and relationship between RTU measures and real carcass measures represented meat quality. Traits considered were longissimus muscle area, fat thickness, and marbling score imaged and scanned using RTU by month and those of carcass measures at about 24 month of age. On this study, increasing pattern for longissimus muscle area would be closely related to growth pattern (p<0.01) and it of fat thickness would be related to quadratic effect of ages. Marbling score would be related to aging, that is, individual deviates of scores were distinctly characterized after 17 mo. even though they were started at about 11 mo. Correlation estimates between RTU measures and corresponding carcass traits were over 0.6 after 17 month of age for all traits considered and these estimates were high as 0.90 after 22 month of age. From this study, Real-time ultrasound measurement would be valuable after 15-17 mo. and measures at about 22 mo. would be helpful to decide age for slaughtering. To utilize these techniques for beef cattle breeding via progeny testing program, 15 mo. RTU measures would be efficiency rather than 12 mo. of other beef breeds. Further study to find genetic variation of RTU measures was needed to utilize these technologies for beef breeding.

Current status of sweetpotato genomics research (고구마 유전체 연구현황 및 전망)

  • Yoon, Ung-Han;Jeong, Jae Cheol;Kwak, Sang-Soo;Yang, Jung-Wook;Kim, Tae-Ho;Lee, Hyeong-Un;Nam, Sang-Sik;Hahn, Jang-Ho
    • Journal of Plant Biotechnology
    • /
    • v.42 no.3
    • /
    • pp.161-167
    • /
    • 2015
  • Sweetpotato [Ipomoea batatas (L.) Lam] grows well in harsh environmental conditions, and is cultivated as one of the top seven food crops in the world. Recently, sweetpotato is drawing interest from people as a healthy food because it is high in dietary fiber, vitamins, carotenoids and overall nutrition value. However, few studies have been conducted on sweetpotato genome sequencing in spite of its importance. This review is aimed at increasing the efficiency of sweetpotato genome sequencing research as well as establishing a base for gene utilization in order to control useful traits. Recently, animal and plant genome sequencing projects increased significantly. However, sweetpotato genome sequencing has not been performed due to polyploidy and heterogeneity problems in its genome. Meanwhile research on its transcriptome has been conducted actively. Recently, a draft of the diploid sweetpotato genome was reported in 2015 by Japanese researchers. In addition, the Korea-China-Japan Trilateral Research Association of Sweetpotato (TRAS) has conducted research on gene map construction and genome sequencing of the hexaploid sweetpotato Xushu 18 since 2014. The Bill & Melinda Gates Foundation launched the 'sweetpotato genomic sequencing to develop genomic tools for Sub-Sahara Africa breeding program'. The chloroplast genome sequence acquired during sweetpotato genome sequencing is used in evolutionary analyses. In this review, the trend of research in the sweetpotato genome sequencing was analyzed. Research trend analysis like this will provide researchers working toward sweetpotato productivity and nutrient improvement with information on the status of sweetpotato genome research. This will contribute to solving world food, energy and environmental problems.

Genotype and Environment Influence on Raffinose and Stachyose Content of Soybean Seed (콩 종자의 Raffinose 및 Stachyose 함량에 대한 유전자형과 환경의 영향)

  • Sung, Mi Kyung;Han, Sung Jin;Seo, Hyung Jin;Choi, Sang Woo;Nam, Sang Hae;Chung, Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.59 no.3
    • /
    • pp.319-324
    • /
    • 2014
  • Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was $2.68{\pm}0.21-5.87{\pm}2.43$ in year 1 and was $3.24{\pm}0.37-9.05{\pm}0.16$ in year 2. The stachyose content (g/kg) was $4.23{\pm}0.98-27.68{\pm}9.90$ at year 1 and was $5.11{\pm}1.09-25.32{\pm}0.35$ in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation ($R^2=0.1985^*$) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.

Evaluation of Potato Genetic Resources and Development of Potato Varieties with Diverse colors (감자 유전자원 평가 및 다양한 컬러 감자 품종 개발)

  • 임학태;이규화;구동만;양덕춘;전익조
    • Korean Journal of Plant Resources
    • /
    • v.16 no.3
    • /
    • pp.264-274
    • /
    • 2003
  • Many potato genetic resources have been collected and improved for their diverse traits over the years using breeding program in KPGR. To select potential varieties for table and processing in Korea, 58 elite potato breeding lines and several 'Valley' varieties were cultivated and harvested at Korea Alpine area in 2001. The cultivated lines and varieties were evaluated using their cultural adaptability in the environment and tuber characteristics, such as the depth of tuber eye, tuber shape, skin color, flesh color, scab resistance, yield, and the resistance of hollow heart and internal brown spot disease. Additionally, in the selection of potential processing varieties, reducing sugar content (sum of glucose and fructose concentration) of tubers is critically considered, because it mainly influence on the chip color of processing potato tuber. For table stock varieties with white skin color, 'Early Valley', 'Summer Valley', 'Winter Valley', and 'Taebok Valley' were selected. In the aspect of diverse potato tuber color, several varieties were selected such as 'Golden Valley' for its yellow fresh and skin color, 'Gogu Valley', 'Juice Valley', and 'Rose Valley' for their red skin color, and 'Purple Valley' for its purple skin. Compared with world wide known processing cultivar 'Atlantic', 24 lines (or varieties) were selected for the potential potato processing industry due to their low reducing sugar contents (below 0.3%), high yield (above 4.0 ton/ha), and unique chip colors. Selected white chipping varieties were 'Taedong Valley', 'Kangshim Valley', and 'Kangwon Valley', which have 0.23%, 0.27%, and 0.29% of reducing sugar contents, respectively. 'Bora Valley', having deep purple color in both skin and fresh, was selected for purple chip variety and has 0.26% of reducing sugar content. Light yellow chip varieties (lines) were 'Rose Valley' and Valley 54, having 0.19% and 0.269% of reducing sugar content, respectively. For French frying potatoes, 'Stick Valley' of 0.22% and Valley 72 of 0.151% in reducing sugars were selected. All of these selected lines and 'Valley' varieties can be used as parents to improve potato genetic resources and to develop better varieties with unique traits and colors.

A New Auricleless Barley Cultivar "Dami" for Whole Crop Forage (밀식적응 양질 다수성 무엽이 청보리 품종 "다미")

  • Park, Tae-Il;Seo, Jae-Hwan;Han, Ouk-Kyu;Park, Ki-Hun;Choi, Jae-Seong;Kim, Jung-Gon;Park, Jong-Chul;Kim, Hong-Sik;Heo, Hwa-Young;Baek, Seong-Bum;Kwon, Young-Up;Park, Hyoung-Ho;Kang, Moon-Seok;Park, Kwang-Geun;Suh, Sae-Jung
    • Korean Journal of Breeding Science
    • /
    • v.41 no.3
    • /
    • pp.349-353
    • /
    • 2009
  • We developed a new barley cultivar "Dami" (Hordeum vulgare L.) with the auricleless gene lig (al, li, aur-a). The characteristic of auricleless is a spontaneous mutant type which has known as a monogenic recessive gene. The plant with the gene has erect leaf blades because of no auricle. The cultivar was derived from a cross between 'BGS60' and 'Kangbori'. 'BGS60' has the auricleless gene (li), while 'Kangbori' showed a high biomass with winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus). Subsequent generations were handled by the bulk method in a pedigree selection program. A promising line showed both high yield and lodging resistance in the yield trials at Iksan in 2003 to 2004, and designated as Iksan414. The line was subsequently evaluated for winter hardiness, earliness, and yield in the seven locations around Korea for three years from 2005 to 2007 and was designated as "Dami" and released. It has the growth habit of III, erect plant type, green leaf and stem similar to the check cultivar 'Sunwoo' Its heading date was April 30, and maturing date May 31 in paddy field conditions, which were similar to those of 'Sunwoo' respectively. The cultivar Dami was 97 cm in culm length, had 643 spikes per $m^2$ and higher leaf dry weight, and better adaptability to dense planting, winter hardiness, and resistance to BaYMV than the check cultivar did. The average forage yield of "Dami" was about 12 ton $ha^{-1}$ in dry matter (33 ton $ha^{-1}$ in fresh matter) in paddy field. "Dami" also showed 7.5% of crude protein content, 28.5% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.4% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. This cultivar would be suitable for the area where the daily minimum temperature of January is above $-8^{\circ}C$ in Korean peninsula.

Studies on Growth Characteristics and Propagation Method of Introduced Hop (Humulus lupulus L.) Cultivars (홉(Humulus lupulus L.) 도입 품종의 생육특성 및 영양번식 연구)

  • Tae Hyun Ha;Jae Il Lyu;Jun-Hyung Lee;Jaihyunk Ryu;Sang Hoon Park;Si-Yong Kang
    • Korean Journal of Plant Resources
    • /
    • v.36 no.2
    • /
    • pp.181-190
    • /
    • 2023
  • Domestic hop (Humulus lupulus L.) production has been suspended since the early 1990s due to foreign imports, but interest in local production is rising due to the recent craft beer boom in Korea. This study was conducted focusing on the development of growth characteristics and propagation technology for 6 introduced hop cultivars as a basic study for domestic hop production and breeding program. In the hop growth survey conducted in 2021 and 2022, the 5-year-old plants after planting generally showed a tendency to increase the height of strobile setting, strobile size, number and weight of strobile per hill compared to the 4-year-old plants. As a result of the experiment with hop vine cuttings, the average rooting rate of all cultivars was as high as 88% even in only water treatment that were not added with Atonik (Atonik, Arysta, Japan), a rooting agent. There were differences between cultivars in rooting length and rooting rate according to the Atonik treatment method. When checking the survival rate of the rooted cuttings seedlings after transplanting into the soil, it was confirmed that the survival rate of the cuttings in the tissue culture room was significantly lower than that of the cuttings in the greenhouse. However, in transplanting step, cutting plants from culture room condition was strongly inhibited plant growth because of changing environment conditions. As a results of tissue culture, the thidiazuron (TDZ) 1 ㎎/L treatment in the media generated 6 to 9 shoots/explant, while the 6-benzylaminopurine (BAP) 1 ㎎/L treatment generated only 1 to 2 shoots/explant. Therefore, it is more effective to culture by adding TDZ rather than BAP. These results indicated that the development of technology to manage stably after transplanting of cutting or micropropagating plants into potting soil is important for mass propagation of hops.

National Genetic Evaluation (System) of Hanwoo (Korean Native Cattle)

  • Park, B.;Choi, T.;Kim, S.;Oh, S.H.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.26 no.2
    • /
    • pp.151-156
    • /
    • 2013
  • Hanwoo (Also known as Korean native cattle; Bos taurus coreanae) have been used for transportation and farming for a long time in South Korea. It has been about 30 yrs since Hanwoo improvement began in earnest as beef cattle for meat yield. The purpose of this study was to determine the trend of improvement as well as to estimate genetic parameters of the traits being used for seedstock selection based on the data collected from the past. Hanwoo proven bulls in South Korea are currently selected through performance and progeny tests. National Hanwoo genetic evaluations are implemented with yearling weight (YW), carcass weight (CW), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS). Yearling weights and MS are used for selecting young bulls, and EMA, BF, and MS are used for selecting proven bulls. One individual per testing room was used for performance tests, and five individuals per room for progeny tests. Individuals tested were not allowed to graze pasture, but there was enough space for them to move around in the testing room. Feeds including roughages and minerals were fed ad libitum, and concentrates were provided at the rate of about 1.8% of individual weight. Overall means of the traits were $352.8{\pm}38.56$ kg, $335.09{\pm}44.61$ kg, $77.85{\pm}8.838\;cm^2$, $8.6{\pm}3.7$ mm and $3.293{\pm}1.648$ for YW, CW, EMA, BF and MS. Heritabilities estimated in this study were 0.30, 0.30, 0.42, 0.50 and 0.63 in YW, CW, EMA, BF and MS, respectively, which are similar to results from previous research. Yearling weight was 315.54 kg in 1998, and had increased to 355.06 kg in 2011, resulting in about 40 kg of improvement over 13 yrs. YW and CW have improved remarkably over the past 15 yrs. Breeding values between 1996 and 2000 decreased or did not change much, but have moved in a desirable direction since 2001. These improvements correspond with the substantial increase in use of animal models since the late 1990s in Korea. Hanwoo testing programs have practically contributed to the improvement in aspects of quality and quantity. In sum, the current selection system is good enough to accommodate circumstances where fewer sires are used on many more cows. Although progeny tests take longer and cost more, they seem to be appropriate under the circumstances of the domestic market with its higher requirement for better meat quality. Consequently, accumulative data collection, genetic evaluation model development, revision of selection indices, as well as cooperation among farms, associations, National Agricultural Cooperative Federation, universities, research institutes, and government agencies must be applied to the Hanwoo selection program. All these efforts will assist the domestic market to secure a competitive position against imported beef under Free Trade Agreement trade system and will provide farmers with higher profits as well as the public with a higher quality of beef.

Geographical Variation in Bud-burst Timing of Zelkova serrata Provenances (느티나무 산지별 개엽시기의 지리적 변이)

  • Kim, In Sik;Han, Sang Urk;Lee, Wi Young;Na, Sung Joon
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.15 no.3
    • /
    • pp.191-200
    • /
    • 2013
  • This study was conducted to examine the geographic variation of bud phenology of Zelkova serrata provenances. Data were collected from Gangneung, Yilmsil, Hwaseong and Jinju plantations which were parts of the 6 provenance trials established by Korea Forest Research Institute in 2009. The 16 provenances were included in these trials. The starting date of bud burst and finishing date of leaf expansion were investigated from April to May every other day. The four geographic factors and fifteen climatic factors of the test sites and provenances were considered in this study. Canonical correlation analysis was conducted to examine the major factors affecting the bud phenology between test sites and provenances. The study results suggested that the major factors affecting the timing of bud burst were the differences of extremely high temperature (March-October), annual mean temperature, mean temperature (March-October), extremely high temperature (July-August) and mean humidity (June-October) between test site and provenance. The provenances with lower mean or high temperature than those of plantation showed the earlier bud burst and leaf expansion. It showed a typical north-south or low-high temperature cline. Finally, we discussed the implication of the tree breeding program of Z. serrata based on these results.