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Studies on Determination of Larval Critical Weight in Silkworm, Bombyx mori L. - An Index of Quality

  • Saha, Atul Kumar;Chaudhuri, Anath Bandhu;Moorthy, S Monthira;Roy, Subrata
    • International Journal of Industrial Entomology and Biomaterials
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    • 제19권2호
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    • pp.243-248
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    • 2009
  • The Larval critical weight is the minimal mass at which further growth in not necessary for a normal time course to pupation. Larval critical weight (Lcw), pupal critical weight (Pcw) and adult critical weight (Acw) of different breeds of Bombyx mori L. were determined through starvation (food deprivation). The Lcw was found to be about 938.46, 2397.26, 2283.57 and 2220.97 mg in males and 1118.15, 2681.04, 2604.9 and 2455.88 mg in females of the multivoltine breed (Nistari), Bivoltine breeds (P5 & NB18) and their hybrid (P5$\times$NB18) respectively. Bivoltine breed P5 took more time (3.35 days) followed by NB18 (3.13 days) & P5$\times$NB18 (3.02 days) to attain larval critical weight (In $5^{th}$ Instar) than the multivoltine breed Nistari (2.42 days). Decrease in weight from larval maximal weight to pupal weight and to adult weight was also observed more in multivoltine than bivoltines, which may be due to more latent feeding period in bivoltines. Since Lcw is a stable character and independent of environment, it could be utilized for characterization of silkworm breeds to assess the quality of an insect.

A Least Squares Regression Model to Detect Quantitative Trait Loci with Polar Overdominance in a Cross of Outbred Breeds: Simulation

  • Kim, Jong-Joo;Dekkers, Jack C.M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권11호
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    • pp.1536-1544
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    • 2013
  • A least squares regression interval mapping model was derived to detect quantitative trait loci (QTL) with a unique mode of genomic imprinting, polar overdominance (POD), under a breed cross design model in outbred mammals. Tests to differentiate POD QTL from Mendelian, paternal or maternal expression QTL were also developed. To evaluate the power of the POD models and to determine the ability to differentiate POD from non-POD QTL, phenotypic data, marker data and a biallelic QTL were simulated on 512 F2 offspring. When tests for Mendelian versus parent-of-origin expression were performed, most POD QTL were classified as partially imprinted QTL. The application of the series of POD tests showed that more than 90% and 80% of medium and small POD QTL were declared as POD type. However, when breed-origin alleles were segregating in the grand parental breeds, the proportion of declared POD QTL decreased, which was more pronounced in a mating design with a small number of parents ($F_0$ and $F_1$). Non-POD QTL, i.e. with Mendelian or parent-of-origin expression (complete imprinting) inheritance, were well classified (>90%) as non-POD QTL, except for QTL with small effects and paternal or maternal expression in the design with a small number of parents, for which spurious POD QTL were declared.

Implications of High Temperature and Low Humidity on the Hatching in the Silkworm, Bombyx mori L. Under LD 12 : 12 Condition

  • Reddy P. Lakshminarayana;Naik S. Sankar;Reddy N. Sivarami
    • International Journal of Industrial Entomology and Biomaterials
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    • 제11권2호
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    • pp.139-143
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    • 2005
  • The implications of temperature (25, 30 and $35^{\circ}C$) and relative humidity (RH; 60, 70 and $80\%$) on the hatching rhythmicity and hatching parameters (percentage and duration) were studied in the silkworm, Bombyx mori L. under natural photoperiod (LD 12 : 12). Disease free layings (DFLs) of two pure silkworm breeds, Pure Mysore (PM, a multivoltine breed) and $NB_4D_2$ (a bivoltine breed), and their hybrid, $PM{\times}NB_4D_2$ were introduced into the experimental conditions on the $3^{rd}$ day of oviposition till completion of hatching. The hatching rhythm was predominantly diurnal under all temperature and humidity conditions, with peaks just after 'lights-on' phase (6 hrs). Extreme temperature and humidity conditions did not alter the hatching rhythmicity, but prolonged the hatching durations, extending it to the next day, coupled with reduced hatching percentage in PM and $PM{\times}NB_4D_2{\cdot}In\;NB_4D_2$, on the other hand, hatching did not extend to the next day. Hatching percentage in this breed, however, reduced below the economic level under high temperature and low humidity conditions. The high temperature and low humidity together, though did not alter the rhythmicity, seems to exert synergetic effect on the hatching percentage and its duration in the silkworm, B. mori.

Extensive Rib Osteosarcoma with Intraperitoneal Invasion in a Small Breed Dog: Clinical and Histopathologic Findings

  • Lim, Chae-Young;Yoo, Jong-Hyun;Park, Chul;Jung, Dong-In;Kim, Ju-Won;Kang, Byeong-Teck;Kim, Ha-Jung;Sur, Jung-Hyang;Park, Hee-Myung
    • 한국임상수의학회지
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    • 제25권3호
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    • pp.182-186
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    • 2008
  • An 11-year-old mixed breed male dog was presented with a thoracoabdominal wall mass of 3-year duration. Initial tentative diagnosis of osteosarcoma was made. Despite chemotherapy treatment, 72 days following the date of presentation, the dog was euthanized. Based upon necropsy and histopathologic findings, the tumor was definitely diagnosed as a combined type osteosarcoma of the rib. At necropsy examination, the tumor extended the left kidney and diaphragm, but distant metastasis was not found. The tumor's weight was 2.3kg and that was 38.3% of the dog's weight. This case report describes the clinicopathological, computed tomographic, histopathologic and immunohistochemical findings of extensive rib osteosarcoma in a small breed dog.

Variations in Karyotypic Characteristics of Different Breed Groups of Water Buffaloes (Bubalus bubalis)

  • Bondoc, O.L.;Flor, M.C.G.T.;Rebollos, S.D.N.;Albarace, A.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권3호
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    • pp.321-325
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    • 2002
  • Karyotype analysis was carried out on blood samples of 30 water buffaloes belonging to different breed groups (i.e. Philippine Carabao (PC), Indian Murrah (IM), Bulgarian Murrah (BM), "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC" and "75% IM-25% PC"), using the modified Leucocyte Culture Technique. The modal chromosome numbers of the PC, "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC", IM, BM and "75% IM-25% PC" were 2n=48, 49, 49, 50, 50 and 50, respectively. The water buffalo chromosomes are mostly acrocentric (79.67%) and the remainder submetacentric (20.33%). Results of the ordinary least square analysis showed significant breed effects (p<0.01) on other karyotypic characteristics (i.e. relative length, arm ratio and centromeric index). Significant correlation between karyotypic characteristics and some animal performance traits were also found. The significant correlation values imply that karyotypic characteristics can be used as important criteria to select potentially productive young water buffaloes. In the future, more production and reproduction traits from non-institutional herds should be included in the analysis to reveal meaningful correlations with various karyotypic characteristics.

Evaluation of Single Nucleotide Polymorphisms (SNPs) Genotyped by the Illumina Bovine SNP50K in Cattle Focusing on Hanwoo Breed

  • Dadi, Hailu;Kim, Jong-Joo;Yoon, Du-Hak;Kim, Kwan-Suk
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권1호
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    • pp.28-32
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    • 2012
  • In the present study, we evaluated the informativeness of SNPs genotyped by the Illumina Bovine SNP50K assay in different cattle breeds. To investigate these on a genome-wide scale, we considered 52,678 SNPs spanning the whole autosomal and X chromosomes in cattle. Our study samples consists of six different cattle breeds. Across the breeds approximately 72 and 6% SNPs were found polymorphic and fixed or close to fix in all the breeds, respectively. The variations in the average minor allele frequency (MAF) were significantly different between the breeds studied. The level of average MAF observed in Hanwoo was significantly lower than the other breeds. Hanwoo breed also displayed the lowest number of polymorphic SNPs across all the chromosomes. More importantly, this study indicated that the Bovine SNP50K assay will have reduced power for genome-wide association studies in Hanwoo as compared to other cattle breeds. Overall, the Bovine SNP50K assay described in this study offer a useful genotyping platform for mapping quantitative trait loci (QTLs) in the cattle breeds. The assay data represent a vast and generally untapped resource to assist the investigation of the complex production traits and the development of marker-assisted selection programs.

Effects of Breed of Sire, Percentage of Bos Taurus Inheritance and Season of Birth on Calving Performance of Crossbred Dairy Cattle

  • Japri, B.M.;Majid, A.M.;Fauziah, H.E.;Adrien, K.R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제10권3호
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    • pp.313-317
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    • 1997
  • Data collected from crossbred dairy cattle at the Institut Haiwan, Kluang, were used to evaluate the effects of breed of sire, percentage of Bos taurus inheritance and season of birth on some calving traits. Breed of sire effect was significant on age at first service (p < 0.01), age at first calving (p < 0.01) and postpartum interval to first service (p < 0.05) but not on calving interval (p > 0.05). Linear contrasts indicate that crossbred-sired cows had first service 593 days earlier and first calving 508 days earlier than purebred-sired cows. Cows sired by Friesians, however, had the earliest first service (466 days) and were among the earliest at first calving (917 days). However, the crossbred-sired cows had slightly longer post-partum interval to first service (13 days longer) and calving interval (20 days longer). Percentage of B. taurus inheritance affected (p < 0.01) all the calving traits studied. In general, the cows with 50% inheritance had the best calving performance. Season of birth had no effect (p > 0.05) on them.

Dairy Potential of Pabna Cows and Crossbreds with Sahiwal and Friesian and Within- and Between-Breed Sire Effects

  • Hoque, M.A.;Amin, M.R.;Hussen, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권2호
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    • pp.161-164
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    • 1999
  • Dairy performances of Pabna cows and its crossbreds with Sahiwal (SL) and Friesian (F) in the Baghabarighat dairying area of Bangladesh were evaluated. Four SL and three F sires were used for the crossbreeding. The exotic sire effect of individuals on their daughters were also evaluated in this study. It showed that the genetic group had a significant (p<0.05) effect on birth weight, age at first heat, calving interval, lactation length and lactation yield. The lowest birth weight (17.9 kg), longest age at first heat (39.2 m) and calving interval (16.3 m) shortest lactation length (199 d) and lowest lactation yield (728 L) were found in Pabna cows. The highest birth weight (22.5 kg). shortest age at first heat (25.5 m) and calving interval (13.7 m), longest lactation length (253 d) and highest lactation yield (1936 L) were found in the F crossbreds. Within the same breed, the effect of individual sire was significant (p<0.05) on age at first heat, lactation length and lactation yield. Individual sire effects of SL bulls differed significantly (p<0.05) but F sires had a non-significant (p>0.05) effect on calving interval in their progeny. The fat % and SNF % in milk and number of services required for conception did not differ (p>0.05) among inter-and intra- breed sire groups.

Chinese Holstein Cattle Shows a Genetic Contribution from Native Asian Cattle Breeds: A Study of Shared Haplotypes and Demographic History

  • Ferreri, Miro;Gao, Jian;Wang, Zhi;Chen, Liben;Su, Jingliang;Han, Bo
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권8호
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    • pp.1048-1052
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    • 2011
  • The Chinese Holstein cattle breed, an introduced breed in China, has been crossbred with native cattle breeds. We hypothesised that the Chinese Holstein local population in Beijing share haplotypes with native Asian cattle breeds, the result of a sudden population expansion in the recent past. We also hypothesised that crossbreeding and population expansion left traces that shaped the genetic makeup of the breed. Evaluation of this was performed by mitochondrial DNA (mtDNA) sequence analysis of Chinese Holstein cattle from Beijing (n = 41) and a comparison of them with the published mtDNA sequences (n = 293) of 14 Asian breeds with an emphasis on Chinese native cattle breeds. Three shared common haplotypes between Chinese Holstein cattle and native Asian cattle were found. Moreover, a high level of haplotype diversity in Chinese Holstein cattle (h = 0.9557) and low nucleotide diversity (${\pi}$ = 0.0052) was found, indicating a past population bottleneck followed by rapid population growth. These findings are supported by the significantly negative deviation of Tajima's D (-1.82085), the star-like pattern of dominant haplotypes and the pairwise mismatch distribution analysis, which showed a unimodal pattern.

종돈의 사회성 위해행동 발현에 관한 연구 (A Study on the Expression of Harmful Social Behaviours in Breeding Pig)

  • 김두환;하덕민;전중환;송준익;문상호
    • 한국축산시설환경학회지
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    • 제19권2호
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    • pp.149-154
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    • 2013
  • This study was conducted to investigate the expression of harmful social behaviours in breeding pigs. Totally 64 pure breed pigs were assigned to determine the effect of breed (Yorkshire and Duroc) and sex (male and female) on the expression of each harmful social behaviours at the GGP level commercial breeding farm. Sucking, bothering, fighting and pushing behaviour were selected the notable harmful social behaviour. The most prevailing and the highest proportion of time spent in harmful social behaviours were bothering and sucking, respectively. Total frequency of bothering and fighting were more in Duroc than Yorkshire. Yorkshire had more proportion of time spent in sucking whereas fighting was more in Duroc. According to sex, female pigs had more frequency of sucking and pushing whereas fighting was significantly more in male pigs. The female pigs showed more time spent in sucking whereas bothering and fighting was more in male pigs.