• Title/Summary/Keyword: Brassica pekinensis

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A Co-expression Network of Drought Stress-related Genes in Chinese Cabbage

  • Lee, Gi-Ho;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.35 no.2
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    • pp.243-251
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    • 2017
  • Plants have evolved to adapt to abiotic stresses, such as salt, cold, and drought stress. In this study, we conducted an in-depth analysis of drought resistance mechanisms by constructing a gene co-expression network in Chinese cabbage (Brassica rapa ssp. pekinensis L.). This drought stress co-expression network has 1,560 nodes, 4,731 edges, and 79 connected components. Based on genes that showed significant co-expression in the network, drought tolerance was associated with the induction of reactive oxygen species removal by raffinose family oligosaccharides and inositol metabolism. This network could be a useful tool for predicting the functions of genes involved in drought stress resistance in Chinese cabbage.

Turnip Mosaic Virus Isolated from Rorippa islandica Borb (속속이풀(Rorippa islandica Borb.)에서 분리한 순무 모자이크 바이러스)

  • 최준근;최국선;최장경;유병주;정태성
    • Korean Journal Plant Pathology
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    • v.10 no.2
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    • pp.136-139
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    • 1994
  • Turnip mosaic virus (TuMV) was isolated from Rorippa islandica showing mild mosaic symptom in growing field of Chinese cabbage and radish. Identification of the virus was based on host range, transmission by aphids, electron micrograph, serological reaction and hybridization detection. The virus systemically infected on Chenopodium quinoa, Nicotiana clevelandii, N. glutinosa, Brassica rapa, B. campestris subsp. pekinensis and Raphanus sativus, whereas showed local infection on C. amaranticolor, Gomphrena globosa and Tetragonia tetragonoides. The virus was transmitted by aphid (Myzus persicae). The virus particle was filamentous with 720$\times$12 nm in length, and reacted positively with an antiserum of TuMV in agar gel double duffusion test. In slot-blot hybridization using the digoxigenin(DIG)-labeled RNA probe, TuMV-RNA could be detected in sap of R. islandica infected with the virus. This is the first report of a natural infection of that virus on R. islandica.

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GC-MS Analysis of the Extracts from Korean Cabbage (Brassica campestris L. ssp. pekinensis ) and Its Seed

  • Hong, Eunyoung;Kim, Gun-Hee
    • Preventive Nutrition and Food Science
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    • v.18 no.3
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    • pp.218-221
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    • 2013
  • Korean cabbage, a member of the Brassicaceae family which also includes cauliflower, mustard, radish, and turnip plants, is a crucial leafy vegetable crop. Korean cabbage is harvested after completion of the leaf heading process and is often prepared for use in "baechu kimchi", a traditional Korean food. Many of the components in Korean cabbage are essential for proper human nutrition; these components can be divided into two groups: primary metabolites, which include carbohydrates, amino acids, fatty acids, and organic acids, and secondary metabolites such as flavonoids, carotenoids, sterols, phenolic acids, alkaloids, and glucosinolates (GSLs). Using gas chromatography-mass spectrometry, this study examined the variety of volatile compounds (including isothiocyanates) contained in Korean cabbage and its seed, which resulted in the identification of 16 and 12 volatile compounds, respectively. The primary volatile compound found in the cabbage was ethyl linoleolate (~23%), while 4,5-epithiovaleronitrile (~46%) was the primary volatile component in the seed.

Expression of Glutathione Reductase Gene in Transgenic Tobacco Plant (형질전환 담배 식물체에서 Glutathione Reductase 유전자의 발현)

  • 이효신;조진기
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.2
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    • pp.87-90
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    • 2001
  • BcGRl gene encoding cytosolic glutathione reductase of Chinese cabbage (Brassica campestris var. Pekinensis cv. Seoul) was placed under the control of the CaMV 35S promoter and introduced into tobacco (Nicotiana tabacum L. cv. Samsun) via Agrobacterium-mediated transformation. T$_{0}$ 32 independent plants transformed with BcGRl gene were selected with kanamycin and they were confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Northern blot analysis revealed that the constitutive expression of BcGRl gene and there was no relationship between the copy number of introduced gene and the levels of BcGRl transcripts.

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Effects of Feeding Wastes from Brassica Species on Growth of Goats and Pesticide/Insecticide Residues in Goat Meat

  • Ngu, Nguyen Trong;Ledin, Inger
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.197-202
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    • 2005
  • The effects of feeding Brassica vegetable market wastes on intake, body weight changes and pesticide/insecticide residues in products of goats were evaluated in two experiments. In the first experiment (Exp. 1) 16 goats (Bach Thao, 9 to 10 kg, 3 months old, 9 males and 7 females) were fed four diets with leaves either from cabbage (Brassica oleracea var. capitata), cauliflower (Brassica oleracea var. botrytis) or Chinese cabbage (Brassica campestris subsp. pekinensis) with 30% of Para grass. The control group was fed 100% Para grass. All diets contained soybean waste as a supplement and the experiment lasted for 136 days. In the second experiment (Exp. 2) 24 goats (Bach Thao, 12 to 14 kg, all males) were assigned to three treatments in a completely randomised block design based on initial body weight. The goats were fed cabbage waste supplemented with 200 g or 100 g DM (dry matter) of concentrate. Para grass with 100 g DM concentrate supplementation was used as a control group. The experiment lasted for 90 days and at the end of the study, 12 goats were slaughtered for pesticide/insecticide analysis. Due to low DM content (5.3 and 3.7%, respectively) feed intakes of cabbage and Chinese cabbage groups were lower than those of other groups in the experiment. The highest feed intake and body weight gain was obtained when the goats were fed cauliflower (529 g DM/day and 87.5 g/day, respectively). In Exp. 2 total intake of cabbage and concentrate was similar (484 g and 453 g DM/day) whether the goats were fed 100 or 200 g concentrate/day but lower than that of Para grass and concentrate probably due to the low DM content of the cabbage (5.9%). Crude protein intake (79 g to 86 g/day) and body weight gain (70 g to 88 g/day) was not significantly different between treatments. Adding concentrate consequently resulted in higher DM intake than in Exp. 1 but did not result in any higher growth rate. Three of the pesticide/insecticide residues tested were found in cabbage, Alpha-Cypermethrin, Bassa-Fenobucarb and Dimethoate with levels of 0.175, 0.074 and 0.028 mg/kg fresh cabbage respectively. Weight of livers from goats fed cabbage was about 90 g higher than from goats fed Para grass but no pesticide/herbicide residues were found in meat or liver.

Change of Germination Rate for Chili Pepper and Chinese Cabbage Seed in Relation to Packaging Materials and Storage Conditions over 10 Years (보관용기 및 저장조건에 따른 고추 및 배추종자의 10년간 발아율 추이)

  • Soh, Eun Hee;Lee, Woo Moon;Park, Kee Woong;Choi, Keun Jin;Yoon, Moo Kyoung
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.864-871
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    • 2014
  • Seed viability is affected by storage conditions and rapid loss of viability in storage is the major cause for low germination. This study was carried out to examine the effect of packaging materials and storage temperature on seed germination rate over 10 years in two species (Capsicum annuum L. and Brassica rapa L. ssp. pekinensis) and determine effective storage conditions for maintaining seed viability. Seeds were packaged in aluminum poly pouches under vacuum, polyethylene bottles, and paper bags containing silica gel and stored under one of two controlled conditions ($15^{\circ}C$, RH 40% or $5^{\circ}C$, RH 30%) or at ambient condition. Seed germination was recorded at 6-month intervals for 10 years. The seeds of both species showed no decline in viability until 6.5 years at 15 or $5^{\circ}C$, irrespective of packaging materials. However, under ambient conditions, the seeds of chili pepper and Chinese cabbage in paper bags lost viability after 4 and 5 years, respectively. By contrast, seeds of both species in vacuum-aluminum poly pouches exhibited a 99% germination rate after 6 years under ambient conditions. Pepper seeds in the vacuum-aluminum poly pouches maintained a 93% germination rate after 10 years in ambient conditions. These results indicated that a special seed storage facility for maintaining viability of chili pepper and Chinese cabbage seed might not be essential and seed testing would not be necessary for 10 years, if chili pepper and Chinese cabbage seeds were packed in ambient/vacuum-aluminum poly pouches or $5^{\circ}C$/vacuum-aluminum poly pouches.

Growth-Promoting Effects of Vegetable Extracts on Selected Human Lactic Acid Bacteria

  • Kim, Moo-Key;Kim, Byung-Su;Baek, Bong-Rea;Shin, Dong-Hwa;Lee, Hoi-Seon
    • Preventive Nutrition and Food Science
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    • v.6 no.3
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    • pp.192-196
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    • 2001
  • Ethanol extracts from 36 vegetable samples were assayed for their growth-promoting effects on Bifidobacterium bifidum, B. longum, and Lactobacillus casei The growth-promoting effects varied according to bacterial strain and vegetable species. In modified Gy rgy broth, extracts of Lactuca sativa, Lycopersicon esculentum and L escutentum var. cerasiforme exhibited strong growth-promoting responses toward B. longum, and significant and strong growth- promoting response toward B. bifidum was observed in extracts of Actinidia arguta, Allium cepa, A. sativum, Brassica campestris subsp. napus vats. pekinensis, Capsicum frutescens, Daucus carota var. sativa, L sativa, 1. esculentum and L. esculentum var. cerasforme, Nelumbo nucifera, Cucurbita moschata, Lackca sativa var. capitata, and Rubus coreanus. For L casei, extracts of A. fshlosum, A. hberosum, Cichorium intbus, Cucurbita moschat\ulcorner Ipomoea batatas, 1. sativa var. capitata, L. esculentum, P. brachycarpa, Raphanus sativus, R. coreanus, and S. melongena strongly enhanced the growth of this bacteria. In modified Gy rgy broth, the promoting effect was most pronounced with B. bifidum and L. casei among lactic acid bacteria used. In MRS broth, A. arguta, A. cepa, A. sativum, B. campestris subsp. napus var. pekinensis, C. frutescens, and D. carota var. sativa L. satiw var. capitata, and R. coreanus strongly enhanced the growth of B. bifidum, Growth of B. longum was strongly affected by the addition of extracts from L. sativa var. capitata. For L casei, moderate growth-promoting responses were observed in 9 vegetable extracts. The promoting effect in MRS broth was most pronounced with B. bifidum among lactic acid bacteria used.

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Effect on Yield of Cabbage and Soil Chemical Properties with Nitrogen and Potash (질소(窒素) 및 가리(加里)의 시용(施用)이 배추의 수량(收量)과 토양화학적(土壤化學的) 성질(性質)에 미치는 영향(影響))

  • Oh, Wang-Keun;Kim, Seoung-Bae;Kang, An-Seok
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.3
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    • pp.253-257
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    • 1984
  • A field experiment was conducted in order to secure the basic information on the rational application of nitrogen and potassium for autumn growing Chinese cabbage (Brassica campastris ssp. pekinensis, var.; Miho 70 days). The results of the experiment are summarized as follows: 1. Potassium effect was observed where nitrogen applied either less than 15kg N/10a and heavy dose of 25kg N/10a. No potassium effect was observed where 20kg N/10a applied. In the case of 25kg N/10a, potassium effect was observed only in total weight but failed yield marketable product weighing over 1 kilogram per cabbage. 2. Nitrogen application, in general, tends to lower the soil pH and it is particularly true when heavy dose of over 20kg N/10a is applied. As a result, Chinese cabbage has increasingly removed soil born potassium and reduced exchangeable potassium content of the soil. 3. Oven dried cabbage which received 25kg N/10a plus potassium showed a low concentration of calcium and this phenomena seems to be attributable to the acidification of soils and it further caused failure in producing cabbages of marketing value.

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Enhancement of Shoot Regeneration by Ethylene Inhibitors from Cotyledon Explant of Brassica campestris L. ssp. pekinensis (에틸렌 저해제에 의한 배추 자엽조직의 기내 재분화율 향상)

  • 이혜승;조화진;김병동
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.267-271
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    • 1995
  • To improve regeneration efficiency of Brassica campestris ssp. pekinensis (chinese cabbage) in vitro, the effect of ehtylene inhibitors [AgNO$_3$ and silver thiosulfate (STS)] and optimal age of explantse were investigated. On the effect of ethylene inhibitors either 100 $\mu$M of AgNO$_3$ or 5 $\mu$M of STS enhanced shoot regeneration from cotyledons when it was added in basal shoot induction media(MS salts, B5 vitamine, sucrose 2%, BA 2.0mg/L, NAA 1.0mg/L). But at higher concentrations, AgNO$_3$ induced abnormal shoots, and STS greatly reduced regeneration frequency. On the other hand, the maximum regeneration rate was obtained from the cotyledons taken from 3-day old seedlings. However there was no distinctive effect among the containers used for cultivation. The most optimal condition of root induction was a minimal Murashige and Skoog media containing 0.1 mg/L NAA. In order to induce bolting and flowering from in vitro regenerated chinese cabbage, the plant were healed at 4$^{\circ}C$ for weeks in a cold chamber. When they were planted in pots, the plane produced phenotipically normal flowers and seeds. The overall results suggest that ethylene inhibitors promote regeneration of shoot from cotyledons of chinese cabbage without alleviating fertility.

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Change of Total Glucosinolates Level according to Processing Treatments in Chinese Cabbage (Brassica campestris L. ssp. Pekinensis) from Different Harvest Seasons (수확기간별 배추의 가공처리에 따른 total glucosinolates함량변화)

  • Kim, Mee-Kyung;Hong, Eun-Young;Kim, Gun-Hee
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.593-599
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    • 2010
  • This study was carried out to investigate the level of total glucosinolates in different parts (outer and inner part) of fresh, salted Chinese cabbage ($Brassica$ $campestris$ L. ssp. $Pekinensis$) and Kimchi at different harvesting periods (June-July, August-September, October-November, December-April, and May). For determination of total glucosinolates, Chinese cabbage was used for analytical sample preparation, provided with an anion exchanges column and measured by UV-visible Spectrophotometer. The fresh Chinese cabbage (FCC) that was harvested in June-July contained the highest level of total glucosinolates and was higher in outer part than inner part in all harvesting periods. The salted Chinese cabbage (SCC) that was harvested in May contained the lowest level of total glucosinolates. Total glucosinolates level of SCC in outer part was higher in June-July and August-September. The manufactured Kimchi (K) using harvested Chinese cabbage in June-July and August-September contained the highest level of total glucosinolates while that harvested in May contained the lowest level. The level of total glucosinolates in different parts was higher in inner part than outer part in all harvesting periods except for May. In all harvesting times, the level of total glucosinolates of FCC was higher than processed Chinese cabbage (SCC and K). Based on these results, levels of total glucosinolates are influenced by harvesting periods, parts and processing conditions of Chinese cabbage.