• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.2
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• pp.49-57
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• 1980

A $F_1$ hybrid out of 143 crosses of rape (Brassica napus) using cytoplasmic-genetic male sterility yielded 5.18 tons per hectare. European and native varieties used, were found out to have 78% and 58% of genes restoring fertility, respectively. The $F_1$ hybrids of three way cross using non-isogenic maintainer showed higher heteroses than those of double cross. To produce F 1 hybrids of three way cross, three stages of crossing operations are needed and seeds produced from about 60 hectares can be covered to 60, 000 hectares.

• Journal of Plant Biology
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• v.25 no.2
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• pp.73-81
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• 1982

The changes in activities of glyoxysomal and peroxisomal enzymes during the transition from fat degradation to photosynthesis were investigated with the cotyledns of rape (Brassica napus L.) seedlings. The development and disappearance of glyoxysomal enzyme (isocitrate lyase, EC 4.1.3.1; malate dehydrogenase, EC 1.1.1.37; catalase, EC 1.11.1.6) activities took place independently of light. It is concluded that the mobilization of storage fat is independent of photomorphogenesis. During early periods of development in the dark of light (days 1 through 3), the glyoxysomal enzyme activities were relatively high and the enzyme activities rose to a peak at 3rd day after sowing. Thereafter, the activities decreased gradually. While glyoxysomal enzyme activities were dropping, the peroxisomal enzyme (glycolate oxidase, EC 1.1.3.1) activities were increasing rapidly during the transition period in the light. Moreover, the changes of enzyme activities of the common microbody marker, catalase, indicated both functional patterns. The enzyme patterns in rape cotyledons indicate that the glyoxysomal function of microbodies is replaced by the peroxisomal function of these organelles during the transition from fat degradation to photosynthesis.

• Journal of Plant Biology
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• v.27 no.2
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• pp.43-50
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• 1984

Effects of IAA on the elongation and cell wall hlysocidase activities were investigated in excised rape (Brassica napus L. cv. Yongdang) hypocotyl segments. IAA promoted the elongation of rape hypocotyl segments. In rape hypocotyls, the first 10-mm segments from the hook exhibited maximal elongation and the capacity of elongation was gradually decreased with increasing distance of each 10-mm from the hook. A good correlation has been obtained between the magnitude of endogenous growth and the activities of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase. However, exogenous application of IAA did not seem to enhance the tissue with IAA resulted in acidification of the incubation medium. From these data, we can conclude that IAA seems to enhance elongation of the tissue segments, at least in part, by releasing hydrogen ion into cell wall, some of which may participate in the cell wall extension process, but does not seem to trigger the activation of $\alpha$, $\beta$-glucosidase and $\alpha$, $\beta$-galactosidase.

• Proceedings of the Korean Society of Crop Science Conference
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• 1994.10a
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• pp.22-23
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• 1994

• Proceedings of the Korean Society of Crop Science Conference
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• 1994.10a
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• pp.24-25
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• 1994

• Proceedings of the Korean Society of Crop Science Conference
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• 1994.10a
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• pp.26-27
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• 1994

• Animal cells and systems
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• v.1 no.1
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• pp.135-142
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• 1997

Random sequencing of expressed sequence tags in roots of Chinese cabbage led to isolation of a partial cDNA clone, BR77, which encoded a putative protein kinase. Using the BR77 cDNA as a probe, we isolated a full-length cDNA encoding the Brassica campestris protein kinase 1 (Bcpk1). The Bcpt1 cDNA contained one open reading frame encoding a polypeptide of 439 amino acids. The putative polypeptide consisted of a short N-terminal region and a protein kinase catalytic domain. The catalytic domain of Bcpkl showed a high homology to cAMP- and calcium- phospholipid-dependent subfamilies of serine/threonine protein kineses. Eleven major catalytic domains in protein kineses were well conserved in Bcpk1. However, Bcpk1 contained a unique nonhomologous intervening sequence between subdomains VII and VIII, which was not found in protein kineses of animals and lower eukaryotes. Genomic DNA gel blot analysis showed that Bcpt1 genes might be present as three copies in the Chinese cabbage genome. These imply that Bcpk1 belongs to a plant-specific serine/threonine protein kinase subfamily.

• Plant Breeding and Biotechnology
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• v.2 no.3
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• pp.289-300
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• 2014

A tissue-specific and developmentally expressed gene was isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis), designated BrREF (B. rapa Rubber elongation factor). BrREF transcripts were expressed at high levels in seedlings and at low levels in flower buds and roots. To study the activity of this promoter, the 2.2 kb upstream sequence of BrREF gene was fused to a β-glucuronidase (GUS) reporter gene and was introduced into Arabidopsis thaliana and B. napus by Agrobacterium-mediated transformation. Strong expression of GUS driven by the BrREF promoter was detected in the cotyledons and hypocotyls of transgenic plant seedlings, but GUS expression was weak in roots, excluding the root tips. GUS expression in the cotyledons and hypocotyls decreased dramatically as the seedlings matured and was not detected in the tissues of mature plants. During floral development, GUS expression was observed in immature anthers. These findings suggest that the BrREF promoter can modulate the tissue-specific and developmental expression of gene at the early stages of growth and development.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.2
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• pp.140-144
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• 2018

To investigate the impact of nitrogen (N) mineral on reproductive potential of Brassica napus L, plants were treated with different levels of N treatment ($N_0$; $N_{100}$; $N_{500}$). The half of N content for each treatment were applied at the beginning of the early vegetative stage and the rest was applied at the late vegetative stage. Nitrogen content in plant tissues such as root, stem and branch, leaf, pod and seed was analyzed and harvest index (HI) was calculated as percentage of seed yield to total plant weight. Biomass and nitrogen content were significantly affected by different levels of N supply. Biomass was significantly decreased by 59.2% in nitrogen deficiency ($N_0$) but significantly increased by 50.3% in N excess ($N_{500}$), compared to control ($N_{100}$). Nitrogen content in all organs was remarkably increased with nitrogen levels. N distribution to stem and branches, and dead leaves was higher in N-deficient ($N_0$) and N excessive plants ($N_{500}$) than in control ($N_{100}$). However, nitrogen allocated to seed was higher in control ($N_{100}$) than in other treatments ($N_0$ or $N_{500}$), accompanied by higher HI. These results indicate that the optimum level of N supply ($N_{100}$) improve HI and N distribution to seed and excessive N input is unnecessary.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.231-237
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survival or regrowth yield. Forage rape (Brassica napus L.) was sown by three seeding densities of 5, 15 and 25cm interval among plants on Sep. 1, 1994. Field-grown plants were sampled on the before wintering (Dec. 4) and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. The dry matter yields from the plots of 5, 15 and 25cm seeding interval were 1,270, 1.01 9 and 1,062 kg/lOa respectively, on the before wintering. The similar pattern wa5 observed in the crude protein yields affected by seeding density. On the before wintering, both of nitrogen and starch contents per plant significantly increased as the seeding density was lowered. Starch content was relatively higher than that of nitrogen in all plots. On the wintering period, the contents of nitrogen reserves were 6.5, 41.2 and 121.7 mglplant, those of starch reserves were 1.0, 5.4 and 185.1 mg/plant, respectively, in the plots of 5, 15 and 25cm seeding interval. Nitrogen reserves on the wintering period increased while starch reserves highly decreased in all plots comparing to the before wintering. 'lhe rates of winter survival were 10.2, 20.6 and 37.1%, and regrowth yields were 76, 96 and 178 kgD.M/ IOa, respectively, in the plots of 5, 15 and 25cm seeding interval. These results cleariy showed that seeding density have a close influence on the level of nitrogen and non-structurd cahohydrate reserves, and that the rate of winter survival and regrowth yield were controlled by reserves level on the wintering period.