• Title/Summary/Keyword: Brassica juncea

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Anxiolytic-like activity of leaf extract of traditionally used Indian-Mustard (Brassica juncea) in diabetic rats

  • Thakur, Ajit Kumar;Chatterjee, Shyam Sunder;Kumar, Vikas
    • CELLMED
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    • v.3 no.1
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    • pp.7.1-7.7
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    • 2013
  • Brassica juncea is a polyphenols enriched edible plant, with diverse medicinal uses of different parts of which have been mentioned in the Ayurveda. The effects of 10 daily oral doses (100, 200, and 400 mg/kg/day) of a methanolic Brassica juncea leaf extract in rat models of anxiety using nondiabetic and alloxan-diabetic rats were quantified. In all the three behavioural tests used, i.e. elevated plus maze, open field, and social interaction tests, anxiolytic-like activity of the extract was observed in the diabetic animals only. Quantitatively, the efficacy of the highest tested dose of the extract in these tests was always less than those observed after its lower ones. These observations provide further experimental evidences for the conviction that Brassica vegetables could as well be useful for combating diabetes associated mental health problems.

A Flavonol Diglucoside from the Leaves of Brassica juncea

  • Choi, Jae-Sue;Kim, Jung-Eun;Kim, Jae-I;Cheigh, Hong-Sik;Yokozawa, Takako
    • Natural Product Sciences
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    • v.6 no.4
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    • pp.199-200
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    • 2000
  • A flavonol diglucoside was isolated from the leaves of Brassica juncea L. The structure of the compound was elucidated as isorhamnetin $3,7-di-O-{\beta}-D-glucopyranoside$ (1) on the basis of chemical and spectral evidence.

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Isolation and Antimicrobial Activity of Antimicrobial Substance Obtained from Leaf Mustard(Brassica juncea) (갓(Brassica juncea)의 항균물질의 분리 및 항균성)

  • 강성구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.5
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    • pp.695-701
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    • 1995
  • The ethanol extract of leaf mustard(Brassica juncea) exhibiting high antimicrobial activities was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The highest antimicrobial activity for the bacteria tested was found in the ethylacetate fraction, but a lesser extent in the butanol fraction. In contrast to antimicrobial activity for the bacteria, both ethylacetate and butanol fractions showed weak antimicrobial activity for yeasts. Unknown compound A in the ethylacetate fraction which exhibited a strong antimicrobial activity was isolated by silica gel column chromatography and HPLC, and exhibited 9 times more antimicrobial activity than the ethylacetate fraction.

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Anti-obesogenic Effect of Brassica juncea Extract on Bisphenol-A Induced Adipogenesis of 3T3-L1 Cells (비스페놀 A (Bisphenol-A)로 유도된 지방세포 분화에 미치는 갓 추출물의 항오비소겐 효과)

  • Lee, Se-jeong;Na, Uoon-Joo;Choi, Sun-Il;Han, Xionggao;Men, Xiao;Lee, Youn Hwan;Kim, Hyun Duk;Kim, Yoon Jung;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.36 no.6
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    • pp.528-536
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    • 2021
  • The purpose of the study was to investigate the content of sinigrin, an index component, in Brassica juncea extract and to evaluate the differentiation of lipocytes, inhibition of production of reactive oxygen species (ROS) and reduction of protein production by lipogenic factors (PPARγ, C/EBPα, aP2) in the processing of Brassica juncea extract and sinigrin in 3T3-L1 preadipocytes which induces Bisphenol A (BPA), an endocrine disrupting environmental hormone. From the investigation, the content of sinigrin in Brassica juncea extract, measured by HPLC, is found to be 21.27±0.2 mg/g. The XTT assay result on BPA-derived 3T3-L1 adipocytes shows there is no cytotoxicity found from 180 µM of sinigrin and 300 ㎍/mL of Brassica juncea extract. Moreover, both intracellular lipid accumulation and ROS production during differentiation of lipocyte are significantly reduced in cells processed with Brassica juncea extract and sinigrin. Lastly, it was also found that the production of transcription factors of lipocyte differentiation, PPARγ, C/EBPα and aP2, were found to be suppressed by the application of Brassica juncea extract and sinigrin. Such results reveals that Brassica juncea is effective in not only suppressing lipid accumulation in the environmental hormone bisphenol A-derived lipocyte, but also in reducing the ROS. The sinigrin-containing Brassica juncea is highly expected to be used in natural functional supplements that prevents the lipid metabolism disorders caused by BPA. There are necessities for additional clinical research and follow-up studies on the in vivo model to verify the relevant mechanisms.

Phytoremediation on the Heavy Metal Contaminated Soil by Hyperaccumulators in the Greenhouse (식물경작장에서의 중금속 고축적종 식물을 이용한 중금속 오염토의 정화 연구)

  • Park, Sang-Hean;Choi, Sang-Il;Park, Jong-Bu;Han, Ha-Kyu;Bae, Sei-Dal;Sung, Il-Jong;Park, Eung-Ryeol
    • Journal of Soil and Groundwater Environment
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    • v.16 no.5
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    • pp.1-8
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    • 2011
  • This study was performed to evaluate the remediation efficiency by Helianthus annuus, Brassica juncea and Brassica campestris on the soil contaminated with nickel, zinc and lead, respectively. The growth rates fell down under 60% in the condition of over 700 mg/kg of zinc for Brassica campestris, 300 mg/kg of lead for Helianthus annuus, and 150 mg/kg of nickel for Brassica juncea on the basis of heavy metal concentration in the soil, because of its toxicity. Also, the hyperaccumulators showed the maximum heavy metal contents in their biomass after 90 days of cultivation. The accumulated heavy metal content per kilogram of hyperaccumulator was 0.65 mg of nickel in Brassica juncea, 0.14 mg of zinc in Brassica campestris, and 0.06 mg of lead in Helianthus annuus, respectively. Additionally, 73.2% of nickel accumulated in Brassica juncea and 95.1% of zinc accumulated in Brassica campestris were concentrated in the upper site of crop like stem and leaves. However, in the case of Helianthus annuus, 83.7% of lead was accumulated in the root.

Effect of Chemical Fertilizer-adaptive Variants, Pseudomonas aeruginosa GRC2 and Azotobacter chroococcum AC1, on Macrophomina phaseolina Causing Charcoal Rot of Brassica juncea

  • Joshi, Kishore Kumar;Kumar, Varun;Dubey, Ramesh Chand;Maheshwari, Dinesh Kumar;Bajpai, Vivek K.;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.25 no.3
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    • pp.228-235
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    • 2006
  • Pseudomonas aeruginosa $GRC_2$, siderophore-producing strain, inhibited growth of Macrophomina phaseolina in vitro and reduced charcoal rot in seeds of Brassica juncea in field when coated with adaptive strains. P. aeruginosa $GRC_2$ and Azotobacter chroococcum $AC_1$ produced indole-3-acetic acid and solubilized insoluble phosphate. A. chroococcum $AC_1$ fixed nitrogen asymbiotically. Urea and diammonium phosphateadaptive variant strains of P. aeruginosa and A. chroococcum strongly inhibited M. phaseolina in comparison to parental strains. Bacterization of seeds induced seed germination, seedling growth, and enhanced yield of B. juncea by 10.87% as compared to full doses of urea and diammonium phosphate. Both adaptive strains of chemical fertilizers aggressively colonized roots, showing effectiveness to growth and developments of B. juncea.

Antioxidant and Antibacterial Activity of Extracts from Brassica juncea czerniak et coss., Celosia cristata L., and Beta vulgaris L. (맨드라미, 비트, 홍갓 색소 추출물의 항산화 및 항균 효과)

  • Kim, Mi-Hye
    • Journal of the Korean Society of Food Culture
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    • v.27 no.6
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    • pp.719-729
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    • 2012
  • We sought to study the qualities and scientific benefits of Dongchimi, a traditional Korean food. We compared and analyzed ingredients used for the appearance and storability of dongchimi - honggot (Brassica juncea czerniak et coss), cockscomb (Celosia cristata L.), and beet Beta vulgaris L.). We specifically examined the antioxidative and antibacterial activity of pigments from extracts of these ingredients. Distilled water ($H_2O$) and 1% citric acid were used to safely extract pigments. The antioxidative activity of the pigments was then measured for total phenolic compounds, SOD (Super Oxide Dismutase), and EDA (Electron Donation Ability) by DPPH. The antibacterial activity of was also assessed by a Paper disc solution. Our results show that the pigments had sufficient antioxidative activity and had antibacterial properties against Gram positive and negative bacteria. In particular, Cockscomb (used for enhancing color) contained the highest amount of polyphenol compounds and had the most efficient antioxidative activity.

Studies on the Anthocyanins in Brassica juncea -Part I. Identification of Anthocyanins- (재래종(在來種)갓의 Anthocyanin 색소(色素)에 관한 연구(硏究) -[제일보(第一報)] Anthocyanin 의 구조추정(構造推定)-)

  • Park, Kun-Hyung
    • Applied Biological Chemistry
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    • v.22 no.1
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    • pp.33-38
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    • 1979
  • The Anthocyanins in the Korean native Brassica juncea, which were favorably used directly for food or as condiments, were investigated. Two kinds of anthocyanin were separated from Korean native Brassica juncea by T.L chromatography and then Robinson test, spectrophotometry, T.L. chromatography, partial hydrolysis were tested for the structural elucidation of these two anthocyanins. It was known that two pigments were peonidin-3-glucoside and peonidin-3-galactoside.

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Plant growth promoting rhizobacteria that decrease chromium toxicity in Brassica juncea

  • M. Rajkumar;Lee, Kui-Jae;Park, Jun-Sik;Park, In-Suk;Lee, Wang-Hui
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.10b
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    • pp.45-45
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    • 2003
  • The aim of the present study was to assess the importance of siderophore producing rhizobacteria on the growth of Brassica juncea under chromium stress. Pseudomonas sp. (A4) produced an iron chelating substance siderophores in iron deficient medium. Under chromium stress condition Pseudomonassp. (A4) markedly increased the root and shoot length and also biomass of Brassica juncea as compared to Pseudomonas sp. (A3). This plantgrowth promotion has been related to the microbial production of siderophore.

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Studies on Compositions and Antifungal Activities of Essential Oils from Cultivars of Brassica juncea L. (갓 정유의 조성 및 항진균작용에 관한 연구)

  • Kang, Chan-Ah;Shin, Seung-Won
    • Korean Journal of Pharmacognosy
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    • v.32 no.2 s.125
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    • pp.140-144
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    • 2001
  • The composition of essential oils in the leaves of three cultivars (Ban-Chung-Gat, Chung-Gat and Dolsan-Gat) of Brassica juncea L. were analyzed and their antifungal activity were investigated in this study. Allyl isothiocyanate, 2-phenyl ethyl isothiocyanate, 4-isothiocyanato-1-butene, 5-methyl isothiazole, benzene acetaldehyde, benzene propane nitrile and beta-ionone have been identified in all of the experimented oils. The main component of the oils from Ban-Chung-Gat and Chung-Gat was 2-phenyl ethyl isothiocyanate while allyl isothiocyanate was the representing compound in the oil of Dolsan-Gat. The antifungal activities of the oils were tested by micro broth dilution method and disc diffusion method. As the result the oils exhibited significant inhibiting activities against Aspergillus niger, A. flavus, Trichoderma viride, Candida albicans, C. utilis, C. tropicalis, Cryptococcus neoformans, Trichosporon mucoides, Trichophyton tonsurans and Geotrichum capitatum.

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