• Title/Summary/Keyword: Brassica crop

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Phytoremediation on the Heavy Metal Contaminated Soil by Hyperaccumulators in the Greenhouse (식물경작장에서의 중금속 고축적종 식물을 이용한 중금속 오염토의 정화 연구)

  • Park, Sang-Hean;Choi, Sang-Il;Park, Jong-Bu;Han, Ha-Kyu;Bae, Sei-Dal;Sung, Il-Jong;Park, Eung-Ryeol
    • Journal of Soil and Groundwater Environment
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    • v.16 no.5
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    • pp.1-8
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    • 2011
  • This study was performed to evaluate the remediation efficiency by Helianthus annuus, Brassica juncea and Brassica campestris on the soil contaminated with nickel, zinc and lead, respectively. The growth rates fell down under 60% in the condition of over 700 mg/kg of zinc for Brassica campestris, 300 mg/kg of lead for Helianthus annuus, and 150 mg/kg of nickel for Brassica juncea on the basis of heavy metal concentration in the soil, because of its toxicity. Also, the hyperaccumulators showed the maximum heavy metal contents in their biomass after 90 days of cultivation. The accumulated heavy metal content per kilogram of hyperaccumulator was 0.65 mg of nickel in Brassica juncea, 0.14 mg of zinc in Brassica campestris, and 0.06 mg of lead in Helianthus annuus, respectively. Additionally, 73.2% of nickel accumulated in Brassica juncea and 95.1% of zinc accumulated in Brassica campestris were concentrated in the upper site of crop like stem and leaves. However, in the case of Helianthus annuus, 83.7% of lead was accumulated in the root.

Molecular characterization of BrRZFPs genes encoding C3HC4 type RING zinc finger protein under abiotic stress from Chinese cabbage (Brassica rapa L.)

  • Jung, Yu Jin;Lee, Kye Dong;Cho, Yong Gu;Nou, Ill Sup;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.40 no.2
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    • pp.102-110
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    • 2013
  • The novel BrRZFPs genes encoding C3HC4-type RING zinc finger protein were identified from FOX (full length cDNA over-expressing) library of Brassica rapa. Ten full-length cDNAs obtained from the library encode zinc-finger protein containing 346 amino acids, designated BrRZFPs. These genes were classified into four groups by phylogenic analysis showing conserved protein sequences at both termini. The tissue distribution of BrRZFPs transcription was examined by qRT-PCR revealing ubiquitous expression pattern. However, each gene was strongly expressed in the specific tissue. Transcriptional analysis showed that those acquired 10 genes were inducible under abiotic stresses. Likewise, the transcript of BrRZFP3 was strongly induced (~12-folds) by exogenous abscisic acid, whereas the transcripts of BrRZFP1, BrRZFP2 and BrRZFP3 were (> 9-folds) induced by cold. We suggest that these BrRZFPs that function as signal or response to abiotic stress are useful for crop improvement.

Resistance to Turnip Mosaic Virus in the Family Brassicaceae

  • Palukaitis, Peter;Kim, Su
    • The Plant Pathology Journal
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    • v.37 no.1
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    • pp.1-23
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    • 2021
  • Resistance to diseases caused by turnip mosaic virus (TuMV) in crop species of the family Brassicaceae has been studied extensively, especially in members of the genus Brassica. The variation in response observed on resistant and susceptible plants inoculated with different isolates of TuMV is due to a combination of the variation in the plant resistome and the variation in the virus genome. Here, we review the breadth of this variation, both at the level of variation in TuMV sequences, with one eye towards the phylogeny and evolution of the virus, and another eye towards the nature of the various responses observed in susceptible vs. different types of resistance responses. The analyses of the viral genomes allowed comparisons of pathotyped viruses on particular indicator hosts to produce clusters of host types, while the inclusion of phylogeny data and geographic location allowed the formation of the host/geographic cluster groups, the derivation of both of which are presented here. Various studies on resistance determination in particular brassica crops sometimes led to further genetic studies, in many cases to include the mapping of genes, and in some cases to the actual identification of the genes. In addition to summarizing the results from such studies done in brassica crops, as well as in radish and Arabidopsis (the latter as a potential source of candidate genes for brassica and radish), we also summarize work done using nonconventional approaches to obtaining resistance to TuMV.

Advances in in vitro culture of the Brassicaceae crop plants

  • Park, Jong-In;Ahmed, Nasar Uddin;Kim, Hye-Ran;Nou, Ill-Sup
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.13-22
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    • 2012
  • Plant regeneration has been optimized increasingly by organogenesis and somatic embryogenesis using a range of explants with tissue culture improvements focusing on factors, such as the age of the explant, genotype, media supplements and $Agrobacterium$ co-cultivation. The production of haploids and doubled haploids using microspores has accelerated the production of homozygous lines in Brassicaceae crop plants. Somatic cell fusion has facilitated the development of interspecific and intergeneric hybrids in sexually incompatible species of $Brassica$. Crop improvement using somaclonal variation has also been achieved. Transformation technologies are being exploited routinely to elucidate the gene function and contribute to the development of novel enhanced crops. The $Agrobacterium$-mediated transformation is the most widely used approach for the introduction of transgenes into Brassicaceae, and $in$ $vitro$ regeneration is a key factor in developing an efficient transformation method in plants. Although many other Brassicaceae are used as model species for improving plant regeneration and transformation systems, this paper focuses on the recent technologies used to regenerate the most important Brassicaceae crop plants.

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

  • Lee, Yong-Hwa;Kim, Kwang-Soo;Jang, Young-Seok;Choi, In-Hu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.2
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    • pp.128-133
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    • 2014
  • Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The 'Tamla' variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. $M_1$ plants were selfed and subsequent generations ($M_2$, $M_3$, and $M_4$ mutants) were analyzed to identify mutants having increased levels of oleic acid. $M_2$ mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv 'Tamla'. We selected two $M_3$ mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected $M_4$ mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.

The Agronomic Growth Characteristics and Fatty Acid Composition in Genetics Resources of Rapeseed (유채(Brassica napus L.) 유전자원의 생육특성과 지방산 조성)

  • Kwang-Soo Kim;Ji Eun Lee;Young Lok Cha;Da Hee An;Dong Chil Chang
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.83-83
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    • 2022
  • 유채(Brassica napus L.)는 가을에 파종하여 이듬해에 초여름에 수확하는 겨울작물로 종실 수량이 많고 종자의 조지방 함량이 높아 주로 기름을 생산하기 위해서 재배되고 있다. 우리나라에서 유채는 경관을 목적으로 주로 재배되며 면적은 약 5,000ha 정도 재배되고 있다. 최근에는 유채유 생산을 목적으로 전남 등 남부지방에서 재배면적이 증가하고 있다. 유채유의 대량 생산을 위해서는 재배과정의 생력기계화에 유리한 논 재배가 주로 이루어지고 있다. 따라서 유채의 육종은 논 재배 적응성이 뛰어나며, 벼와의 작부가 가능하며 봄 파종 재배가 가능한 조숙품종의 육성이 필요하며, 식용유로 이용이 가능한 지방산 조성이 우수한 품종의 육성도 필요하다. 본 연구는 농촌진흥청 농업유전자원센터에서 보유하고 있는 유채 유전자원 350점을 대상으로 작물학적 생육특성을 평가하였고 종자를 수확한 후 지방산 조성을 분석하였다. 생육특성은 경장 등 12항목을 유채 유전자원 특성조사 및 관리요령(RDA, 2011)을 기준으로 조사하였다. 가을에 파종하여 재배할 때 개화소요일수는 파종 후 137일부터 210일까지 소요되었으며, 봄 파종 재배 시에는 파종 후 65일부터 150일까지 개화가 진행되었고 개화가 되지 않은 계통이 67계통이었다. 경장은 85 ~ 211cm, 수장은 28 ~ 79cm, 분지수는 5 ~ 21개, 수당 협수는 29 ~ 106개, 협당 종자수는 18 ~ 35개 및 협장은 2.7 ~ 8.8cm로 다양하였다. 유채 유전자원의 지방산 중 올레산과 에루스산 함량은 각각 9.7 ~ 70.4% 및 0 ~ 54.7% 범위였다. 공시계료 중 IT 279089 등 3자원은 개화기가 빨라 조생종 육성에, IT279125 등 3자원은 올레산 함량이 68%이상으로 양질 지방산 품종 육성재료로 활용할 예정이다.

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