KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

  • Lee, Yong-Hwa (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Kwang-Soo (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Jang, Young-Seok (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, In-Hu (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration)
  • Received : 2013.07.24
  • Accepted : 2014.03.10
  • Published : 2014.06.30
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Abstract

Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The 'Tamla' variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. $M_1$ plants were selfed and subsequent generations ($M_2$, $M_3$, and $M_4$ mutants) were analyzed to identify mutants having increased levels of oleic acid. $M_2$ mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv 'Tamla'. We selected two $M_3$ mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected $M_4$ mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.

Keywords

References

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