• ALGAE
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• v.37 no.2
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• pp.175-183
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• 2022

Polar microorganisms produce physiologically active substances to adapt to harsh environments, and these substances can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica, produced phytol, a natural antimicrobial agent. Furthermore, several polyunsaturated fatty acids (PUFAs), including omega-3, exhibit antioxidant properties. Here statistical methods (Plackett-Burman design and Box-Behnken design) were used to optimize the culture medium of KSF0031 to improve biomass production, and K₂HPO₄, MgSO₄·7H ₂O, and ammonium ferric citrate green (AFCg) were selected as significant components of the culture medium. Changes in the concentration of K₂HPO₄ and MgSO₄·7H ₂O as positive factors and AFCg as a negative factor affected cell growth to a remarkable degree. The biomass production in a 100 L culture using the optimized medium for 24 d at 18℃ was improved by 37.5% compared to that obtained using the original BG-11 medium. The quantities of PUFAs and phytol obtained were 13 mg g^-1 dry cell weight (DCW) and 10.98 mg g^-1 DCW, which represent improved yields of 11.70% and 48.78%, respectively. The results of this study could contribute to an improved production of phytol and fatty acids from Antarctic microalgae in the biomedical industry.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.4
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• pp.567-576
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• 2017

In a performance-based design, the structural safety is estimated from pre- defined damage states and corresponding damage indices. Both damage states and damage indices are well defined for above-ground structures, but very limited studies have been performed on underground structures. In this study, we define the damage states and damage indices of a cut-and-cover box tunnel which is one of typical structures used in metro systems, under a seismic excitation from a series of inelastic frame analyses. Three damage states are defined in terms of the number of plastic hinges that develop within the structure. The damage index is defined as the ratio of the elastic moment to the yield moment. Through use of the proposed index, the inelastic behavior and failure mechanism of box tunnels can be simulated and predicted through elastic analysis. In addition, the damage indices are linked to free-field shear strains. Because the free-field shear strain can be easily calculated from a 1D site response analysis, the proposed method can be readily used in practice. Further studies are needed to determine the range of shear strains and associated uncertainties for various types of tunnels and site profiles. However, the inter-linked platform of damage state - damage index - shear wave velocity - shear strain provides a novel approach for estimating the inelastic response of tunnels, and can be widely used in practice for seismic designs.

• Journal of Ubiquitous Convergence Technology
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• v.2 no.2
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• pp.88-96
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• 2008

IPTV Content delivery systems over wired networks confront scalability problems due to their high network bandwidth requirement for real-time services. Especially, VoD service provides Trick Mode features such as pause, fast forward and similar operations. However, Trick Mode services are delivered by the method of unicast only for controlling of the stream. With a point of views, this paper propose a new real-time multimedia streaming architecture over IP Networks, which tries to achieve bandwidth efficiency and supporting for mass clients better than traditional unicast services. The proposed methods divide the Transport Stream into a series of segments. After that, this divided partial Transport Stream makes multicast streaming periodically. Meanwhile Set-top Box of a client makes a rearrangement orderly by using Presentation Time Stamp field from the served Transport Stream packets. While the current Transport Stream segment is playing, it should be guaranteed that the next segment is downloaded on time. Consequently, the original video content can be played out continuously. The detail introduction of a new real-time multimedia streaming system with analysis and simulation follows as below.

• Journal of the Korean Society of Visualization
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• v.17 no.2
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• pp.48-57
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• 2019

A straightforward strategy for particle image velocimetry (PIV) interrogation and post-processing has been proposed, aiming at reducing errors and clarifying vortex structures. The interrogation window size should be kept small to reduce bias error and improve spatial resolution. A spatial filter is then applied to the velocity field to reduce random error and clarify flow structure. The performance of three popular spatial filters were assessed: box filter, median filter, and local quadratic polynomial regression filter. In order to quantify random uncertainty, the image matching (IM) method is applied to an experimental dataset of homogeneous and isotropic turbulence (HIT) obtained by 2D-PIV. We statistically analyze the uncertainty propagation through the spatial filters, and verify the reduction in random uncertainty. Moreover, we illustrate that the spatial filters help clarify vortex structures using vortex identification criteria. As a result, PIV random uncertainty was reduced and the vortex structures became clearer by spatial filtering.

• Journal of Life Science
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• v.27 no.12
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• pp.1479-1485
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• 2017

Muscle atrophy due to aging, starvation, and various chronic diseases leads to a decrease in muscle fiber area and density due to reduced muscle protein synthesis and increased protein breakdown. This study investigated the effect of dexamethasone and hydrogen peroxide on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes. C2C12 myoblasts were differentiated into myotubes in differentiation medium. During myoblast differentiation, muscle-specific transcription factors, such as myogenin, and MyoD expression increased. Differentiated C2C12 myotubes exposed to noncytotoxic levels of dexamethasone and hydrogen peroxide showed a decrease in myotube diameter, which was associated with up-regulation of muscle-specific ubiquitin ligases, such as muscle atrophy F-box (MAFbx)/atrogin-1 and muscle RING finger-1 (MuRF1), and down-regulation of myogenin and MyoD. These results demonstrated that dexamethasone and hydrogen peroxide induced atrophy through regulation of muscle-specific ubiquitin ligases and muscle-specific transcription factors in C2C12 myotubes. In this study, we confirmed the process of differentiation of C2C12 myoblasts into myotubes in in vitro experiments in the presence of atrophy. This muscle atrophy model of C2C12 cells induced by dexamethasone or hydrogen peroxide seems suited to studies of the mechanism of muscle atrophy suppression and to exploit the experiment for excavating new muscle atrophy.

• Polymer(Korea)
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• v.34 no.2
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• pp.159-165
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• 2010

Dipyridine-terminated polystyrenes and polybutadienes were synthesized by the chain endfunctionalization reaction of polystyryllithium (PSLi) and polybutadienyllithium (PBDLi) with di(2-pyridyl) ketone(DPK) using a living anionic polymerization method in the Ar-glove box. Living polymeric lithiums with low molecular weights (Mw=1000~2000 g/mol) were used to investigate the chain end-functionalization yield with DPK and the degree of coupling reaction by the attack of organolithium to the pyridine ring in the presence of TMEDA using GPC, $^1H$-NMR, $^{13}C$ analysis. DPK-terminated PBD exhibited much higher functionalization yield and less amount of coupling reaction compared with DPK-terminated PS. 86% functionalization yield with 9% degree of coupling was obtained when the PBDLi was added dropwise to DPK solution at room temperature. The functionalization yield was increased as the reaction temperature decreased, however, no LiCl effect was observed in this chain end-functionalization reaction with DPK.

• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.237-242
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• 2009

Pregnancy is a unique event in which a fetus develops in the uterus despite being genetically and immunologically different from the mother, and the underlying mechanisms remain poorly understood. To analyze the differential gene expression profiles in nonpregnant and 7 days post coitus (dpc) pregnant uterus of mice, we performed a global proteomic study by 2-D gel electrophoresis (2-DE) and MALDI-TOF-MS. The uterine proteins were separated using 2-DE, Approximately 1,000 spots were detected on staining with Coomassie brilliant blue. An image analysis using Melanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between pregnant and nonpregnant uterus. Twenty-one spots were identified as differentially expressed proteins, of which 10 were up-regulated proteins such as alpha-fetoprotein, chloride intracellular channel 1, transgelin, heat-shock protein beta-1, and carbonic anhydrase II, while 11 were down-regulated proteins such as X-box binding protein, glutathione S-transferase omega 1, olfactory receptor Olfr204, and metalloproteinase-disintegrin domain containing protein TECADAM. Most of the identified proteins appeared to be related with catabolism, cell growth, metabolism, regulation, cell protection, protein repair, or protection. Our results uncovered key proteins of mouse uterus involved in pregnancy.

• ALGAE
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• v.30 no.2
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• pp.121-137
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• 2015

Studies on carbon flux in the oceans have been highlighted in recent years due to increasing awareness about climate change, but the coastal ecosystem remains one of the unexplored fields in this regard. In this study, the dynamics of carbon flux in a vegetative coastal ecosystem were examined by an evaluation of net and gross ecosystem production (NEP and GEP) and $CO_2$ exchange rates (net ecosystem exchange, NEE). To estimate NEP and GEP, community production and respiration were measured along different habitat types (eelgrass and macroalgal beds, shallow and deep sedimentary, and deep rocky shore) at Gwangyang Bay, Korea from 20 June to 20 July 2007. Vegetative areas showed significantly higher ecosystem production than the other habitat types. Specifically, eelgrass beds had the highest daily GEP ($6.97{\pm}0.02g\;C\;m^{-2}\;d^{-1}$), with a large amount of biomass and high productivity of eelgrass, whereas the outer macroalgal vegetation had the lowest GEP ($0.97{\pm}0.04g\;C\;m^{-2}\;d^{-1}$). In addition, macroalgal vegetation showed the highest daily NEP ($3.31{\pm}0.45g\;C\;m^{-2}\;d^{-1}$) due to its highest P : R ratio (2.33). Furthermore, the eelgrass beds acted as a $CO_2$ sink through the air-seawater interface according to NEE data, with a carbon sink rate of $0.63mg\;C\;m^{-2}\;d^{-1}$. Overall, ecosystem production was found to be extremely high in the vegetated systems (eelgrass and macroalgal beds), which occupy a relatively small area compared to the unvegetated systems according to our conceptual diagram of a carbon-flux box model. These results indicate that the vegetative ecosystems showed significantly high capturing efficiency of inorganic carbon through coastal primary production.

• Journal of Digital Contents Society
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• v.13 no.2
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• pp.225-233
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• 2012

The 3D stereoscopic image contents have been emerging as the blue chip of the contents market of the next generation since the . However, all the 3D contents created commercially in the country have failed to enter box office. It is because the quality of Korean 3D contents is much lower than that of overseas contents and also current 3D post production process is based on 2D. Considering all these facts, the 3D editing process has connection with the quality of contents. The current 3D editing processes of the production case of are using the way that edits with the system on basis of 2D, followed by checking with 3D display system and modifying, if there are any problems. In order to improve those conditions, I suggest that the 3D editing process contain more objectivity by visualizing the depth data applied in some composition work such as Disparity map, Depth map, and the current 3D editing process. The proposed process has been used in the music drama , comparing with those of the film . The 3D values could be checked among cuts which have been changed a lot since those of , while the 3D value of drew an equal result in general. Since the current process is based on an artist's subjective sense of 3D, it could be changed according to the condition and state of the artist. Furthermore, it is impossible for us to predict the positive range, so it is apprehended that the cubic effect of space might be perverted by showing each different 3D value according to cuts in the same space or a limited space. On the other hand, the objective 3D editing by applying the visualization of depth data can adjust itself to the cubic effect of the same space and the whole content equally, which will enrich the 3D contents. It will even be able to solve some problems such as distortion of cubic effect and visual fatigue, etc.

• Journal of Korea Multimedia Society
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• v.21 no.2
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• pp.77-86
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• 2018

Although separation of touching pigs in real-time is an important issue for a 24-h pig monitoring system, it is challenging to separate accurately the touching pigs in a crowded pig room. In this study, we propose a separation method for touching pigs using the information generated from Convolutional Neural Network(CNN). Especially, we apply one of the CNN-based object detection methods(i.e., You Look Only Once, YOLO) to solve the touching objects separation problem in an active manner. First, we evaluate and select the bounding boxes generated from YOLO, and then separate touching pigs by analyzing the relations between the selected bounding boxes. Our experimental results show that the proposed method is more effective than widely-used methods for separating touching pigs, in terms of both accuracy and execution time.