• 한국임상수의학회지
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• 제16권1호
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• pp.19-25
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• 1999

A total of 137 strains of Staphylococcus aureus were isolated from dairy cow's milk with subclinical mastitis from 33 herds in 5 provinces and 36 strains of S aureus from clinical mastitis from 4 herds where the mastitis were severe problem. Arbitrary primed polymerase chain reactions with 10 bp oligonucleotide primer were performed and the PCR products were analysed with image analyzer, The S aureus strains were genotyped into 20 distinct DNA fingerprinting profiles. The size of PCR products ranged from 163 to 2,479 bp and PCR products of 506, 770, 784 and 2,479 bp were the most prevailing bands. Genotype 3 was founded in all 5 provinces. The various genotypes were identified in newly founded dairy herds, however, only one or two genotypes were identified in the closed herds. In clinical mastitis, only a limited number of different S aureus genotype was founded in each of the herds in comparision with subclinical mastitis. The results demonstrated that PCR-based DNA fingerprinting analysis of S aureus strain can be used to study epidemiology of mastitis, in addition, common genotype in geographic region can be useful for the development of an effective S aureus bacterin.

• 대한수의학회지
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• 제15권1호
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• pp.93-99
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• 1975

In order to compare the diagnostic value of Modified California Mastitis Test (MCMT), Modified Whiteside Test (MWT) and Resazurin Reduction Test (RRT) using the direct microscopic leucocyte count (DMLC) as standard, a total of 739 quarter milk samples were examined. The results obtained were as follows: 1. Of the 739 samples, 24.4% had positive DMLC value (over 500,000 leucocytes per mI.), 32.6% positive MCMT reaction, 34.9% positive RRT reaction and 39.9% positive MWT reaction. 2. The identical ratings of the three mastitis screening tests with DMLC values were 60.7% (MWT), 61. 8% (MCMT) and 72.1% (RRT). 3. The mean reaction values of the predicted mastitis screening tests were $1.09{\pm}0.01$ (MWT), $1.12{\pm}0.06$ (MCMT) and $1.25{\pm}0.40$ (RRT). The efficiency ratings of them were 34.8% (MWT), 49.3% (RRT) and 55.0% (MCMT) respectively.

• 대한수의학회지
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• 제28권2호
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• pp.397-404
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• 1988

A total of 593 mastitic pathogens were isolated from the clinical mastitic milk of dairy cattle in Gyeonggi area from March 1984 to February 1988. The mastitic pathogens were further studied bacteriologically and examined for susceptibility patterns to 10 antibiotics. The results obtained were summerized as follows: 1. One hundred and seventy three pathogens were isolated in the first year of studies, 205 pathogens in the second year, 122 pathogens in the third year and 93 pathogens in the last year. 2. The pathogens isolated from clinical mastitis were more in summer than other seasons. 3. Staphylococcus spp (50%) and Streptococcus spp (18%) were the main pathogens in the first year of studies but coliforms (15%) and other bacteria (40%) were the main pathogens in the last year of studies. 4. Coliform mastitis was occurred only in summer and autumn. 5. Antibiotic susceptibility patterns of the same genuses from clinical mastitis infections in different herds and in different years varied greatly. Therefore, treatment should be selected on the basis of susceptibility test results.

• 대한수의사회지
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• 제23권8호
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• pp.537-543
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• 1987

In order to compare the diagnosis values the Rolling Ball Viscometer(RBV) and the California Mastitis Test(CMT) for detection of bovine subclinical mastitis, A total of 2204 quarter milk samples from 565 heads of Holstein in Gyeongnam area were examined d

• Asian-Australasian Journal of Animal Sciences
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• 제16권6호
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• pp.889-893
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• 2003

Immunomodulatory and therapeutic potential of Enrofloxacin was studied in bovine sub clinical mastitis (SCM). The therapeutic efficacy was adjudged by Somatic cell count and Total bacterial count of the milk, whereas, the immuno modulatory potential of the drug was assessed by measuring myeloperoxidase (MPO) and acid phosphates (ACP) enzyme level in the milk leukocytes. Forty-five cows were divided into three equal groups. Gr I consisting 15 cows served as healthy control, whereas, 30 cows (SCM), Gr II and Gr III, selected on the basis of California Mastitis Test (CMT) positive reaction. Gr II cows received 150 mg of Enrofloxacin, once a day for three days and Gr.III received sterile 5 ml PBS (pH 7.4) for 7days, both the treatment were given by intramammary route. The observation was made up to 30 days post-treatment (PT). The CMT of the healthy milk was negative (0), whereas, it ranged between 1 point score and 2 point score in SCM. The Somatic cell count (SCC) and Total bacterial count (TBC) decreased significantly (p<0.05) on day 3 PT in GrII cows in Enrofloxacin treated group, however, such changes were insignificant in PBS treated group. Traces of MPO and ACP enzyme were found in the healthy milk. The mean ACP level enhanced by 70% on day 3 PT in GrII and only 18.7% in Gr. III cows. The mean MPO level enhanced to 32% in Gr. II and 18 % in Gr. III cows on day 3 PT. Concomitant use of Enrofloxacin in SCM at sub optimal dose was found to reduce the bacterial load by increasing the bactericidal enzyme level in the milk polymorphonuclear cells (PMNs) in bovine SCM, which indicates its immunomodulatory potential in mastitis.

• Journal of Veterinary Science
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• 제25권1호
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• pp.6.1-6.11
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• 2024

Background: Chronic bovine mastitis is linked to biofilm-producing Staphylococcus aureus (bp-Sa) or Staphylococcus coagulase-negative (bp-Scn). Objectives: Bp-Sa and bp-Scn were treated with intramammary preparations of either enrofloxacin HCl·2H₂O-dimethyl-sulfoxide-chitosan (enro-C/DMSO/chitosan) or enro-C alone. Their potential to inhibit and degrade biofilm formation in vitro was also assessed. Methods: Milk samples were obtained from the affected quarters in a herd. Phenotypical and genotypical identifications as biofilm-producing Staphylococcus species were carried out. Enro-C/DMSO/chitosan and enro-C alone were assessed to determine their in vitro efficacy in interfering with biofilm formation and their bactericidal effects. A prolonged eight-day treatment with a twice-daily intramammary insertion of 10 mL of enro-C/DMSO/chitosan or enro-C alone was set to evaluate the clinical and bacteriological cures on day 10 in 15 cows per group and the biofilm-inhibiting ability. Results: Fifty-seven percent of the isolates were identified as Staphylococcus spp., of which 50% were bp-Sa, 46% bp-Scn, and 4% Staphylococcus pseudintermedius. One hundred percent of the S. aureus isolated and 77% of Staphylococcus coagulase-negative were biofilm producers. In both groups, the icaA and icaD biofilm-producing genes were identified. The experimental preparation could inhibit biofilm formation, degrade mature biofilms, and have well-defined microbicidal effects on planktonic and biofilm bacteria. The respective clinical and bacteriological cure rates were 100% and 80% for enro-C/DMSO/chitosan and 41.7% and 25% for enro-C alone. Conclusions: Enro-C/DMSO/chitosan eliminates bp-Sa and bp-Scn from cases of chronic bovine mastitis.

• 한국환경농학회지
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• 제27권4호
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• pp.435-440
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• 2008

E. coli, S. agalactiae, S. aureus는 젖소의 유방염을 유발하는 주요 원인균들이다. 이 균들은 분변 혹은 우유에 존재하며, 감염되지 않은 다른 개체로의 감염을 유발한다. 자외선은 소독제로서 이미 산업계 및 의료계에서 쓰레기 및 물의 살균에 사용되고 있으므로, 자외선을 이용하여 젖소의 유방염 확산을 방지하는 것의 실효성을 검증하였다. 분변의 함수율은 젖소 유방염 유발균의 증식에 영향을 미치지 않았고, 144시간 이상의 장시간 생존이 가능함을 확인하였다. 자외선의 조사 시, 조사하는 동안 우분뇨 및 톱밥을 교반하는 것이 교반하지 않는 것보다 균의 증식을 억압하는 것을 확인할 수 있었다. 한편, 자외선의 살균력은 함수율이 낮을수록, 조사시간이 길수록, 조사거리가 짧을수록 더 증가하는 것을 확인할 수 있었다. 본 실험을 통해 얻은 함수율, 조사시간, 조사거리, 교반 여부에 대한 결과는 환경 유래, 특히 우분뇨 유래 젖소 유방염의 감염을 예방하고자 할 때 자외선 살균기의 적용이 가능할 것이며, 현장에 적용할 살균기의 제작에 중요한 기초 자료로서 활용될 것이다.

• 대한수의학회지
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• 제21권2호
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• pp.161-165
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• 1981

A total of 210 dairy cows of Seongwhan area were epidemiologically investigated for mastitis by Milk Quality Test(MQT) method. Milk samples from cows infected with mastitis were also bacteriologically studied. Seventy four (42.5%) of 174 dairy cows were positive for mastitis and fifty nine (79.7%) among them were infected with subclinical mastitis and fifteen (20.3%) were clinical mastitis. High infective rate were shown on August (48.7%) and July (45.7%) and it was lowered on December (35.8%). The incidence rate of subclinical mastitis for herd shown tendency of increase at the barn in which located on lower place (30.7~43.9%), whereas, the barn in which was on higher place and with bucket type milking system shown tendency of decrease causative organisms. A total of 230 strains of etiological agents were isolated from 578 infected duarters which shown MQT score (+) or more. The relevant causative organisms isolated in this study were Staphylococcus aureus (18.3%), Staphylococcus epidermidis (16.9%), fungi (16.5%), Pseudomonas aeruginosa (15.2%), Escherichia coli (7.8%) and Serratia marcescens (2.27), respectively.

• 한국동물위생학회지
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• 제40권3호
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• pp.219-225
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• 2017

This study was investigated the synergistic effects from the combination of cefalexin and gentamicin (CCG) against Staphylococcus spp. isolated from bovine milk. Minimum inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) index were determined for 16 bacteria from 13 bovine farms in Gyeongsangnam province by the National Committee for Clinical Laboratory Standards broth microdilution procedure. Antimicrobial agents tested included cefalexin, gentamicin and CCG. Against Staphylococcus spp. tested, the MIC ranges of cefalexin, gentamicin and CCG were 0.25~1.0, 0.5~0.125 and $0.06{\sim}0.125{\mu}g/mL$, respectively. In addition, the FIC index ranges of CCG against most of the isolates was 0.28~0.43 and the FIC index of CCG against 2 strains of Staphylococcus haemolyticus (S. haemolyticus) was 0.59. In conclusion, CCG has a high antibacterial activity against Staphylococcus isolates, then the combination may be applied for the treatment of bovine mastitis caused by Staphylococcus spp.

• 한국임상수의학회지
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• 제10권1호
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• pp.141-145
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• 1993

The present study was performed in order to clarify the significance of serum amyloid A(SAA) estimation for the diagnosis of bovine amyloidosis and SAA as a useful parameter for the health status in herds. Twelve dutch dairy cows with final diagnosis(2 with amyloidosis, 3 with acute inflammatory disease and 7 with chronic inflammatory disease) were used to charify the significance of SAA determination for the diagnosis of bovine amyloidosis. The SAA concentration in the group of inflammatory disease was higher than that of amyloldotic group. Further the SAA value in the group of acute inflammatory disease was higher than that of chronic ones. To clarify the significance of SAA estimation as a useful parameter for the health status in herds, two Korean dairy farms(A and B) were selected and the SAA concentration was determined in total 76 cows(49 from A farm and 27 from B farm). The SAA concentration in cows from A farm was ranged with 0～169%. The cows with high level of SAA(31～169%) had the disease histories(1 with retained placenta, 3 with chronic mastitis, 2 with acute mastitis, 1 with abortion and acute mastitis, 1 with ovarian dysfunction, 1 with downer cow syndrome and 1 with laceration of the teat). The SAA value in the cows from B farm was ranged with 0～29% and disease history was not detected. In conclusion the SAA determination only is thought to be difficult for the diagnosis of bovine amyloidosis. Furthermore SAA estimation is thought to be a useful parameter for the health status in herds.