Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Synergistic antibacterial effects of the combination of cephalexin with gentamicin against Staphylococci isolated from dairy cows with clinical mastitis in Gyeongnam province

경남지역에서 유방염 젖소로부터 분리한 포도상구균에 대한 세팔렉신과 겐타마이신 합제의 항균상승효과

  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Yoo, Chang-Yeol (Department of Computer Information, Gyeongnam Provincial Namhae College) ;
  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Son, Song-Ee (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University)
  • 차춘남 (경상대학교 공과대학 산업시스템공학과) ;
  • 유창열 (경남도립남해대학 스마트융합정보과) ;
  • 박은기 (고신대학교 인문사회의학교실) ;
  • 손송이 (경상대학교 수의과대학) ;
  • 김석 (경상대학교 수의과대학) ;
  • 이후장 (경상대학교 수의과대학)
  • Received : 2017.06.15
  • Accepted : 2017.07.01
  • Published : 2017.09.30
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Abstract

This study was investigated the synergistic effects from the combination of cefalexin and gentamicin (CCG) against Staphylococcus spp. isolated from bovine milk. Minimum inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) index were determined for 16 bacteria from 13 bovine farms in Gyeongsangnam province by the National Committee for Clinical Laboratory Standards broth microdilution procedure. Antimicrobial agents tested included cefalexin, gentamicin and CCG. Against Staphylococcus spp. tested, the MIC ranges of cefalexin, gentamicin and CCG were 0.25~1.0, 0.5~0.125 and $0.06{\sim}0.125{\mu}g/mL$, respectively. In addition, the FIC index ranges of CCG against most of the isolates was 0.28~0.43 and the FIC index of CCG against 2 strains of Staphylococcus haemolyticus (S. haemolyticus) was 0.59. In conclusion, CCG has a high antibacterial activity against Staphylococcus isolates, then the combination may be applied for the treatment of bovine mastitis caused by Staphylococcus spp.

Keywords

References

  1. Barkema HW, Schukken YH, Zadoks RN. 2006. The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. J Dairy Sci 89: 1877-1895. https://doi.org/10.3168/jds.S0022-0302(06)72256-1
  2. Bergeron M, Dauwalder O, Gouy M, Freydiere AM, Bes M, Meugnier H, Benito Y, Etienne J, Lina G, Vandenesch F, Boisset S. 2011. Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 30: 343-354. https://doi.org/10.1007/s10096-010-1091-z
  3. Bochniarz M, Wawron W. 2011. Antibiotic susceptibility of methicillin-resistant and methicillin-susceptible coagulasenegative staphylococci isolated from bovine mastitis. Pol J Vet Sci 14: 405-410.
  4. Bradley A. 2002. Bovine mastitis: an evolving disease. Vet J 164: 116-128. https://doi.org/10.1053/tvjl.2002.0724
  5. Bradley AJ, Leach KA, Breen JE, Green LE, Green MJ. 2007. Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Vet Rec 160: 253-257. https://doi.org/10.1136/vr.160.8.253
  6. Buyck JM, Tulkens PM, Van Bambeke F. 2015. Activities of antibiotic combinations against resistant strains of Pseudomonas aeruginosa in a model of infected THP-1 monocytes. Antimicrob Agents Chemother 59: 258-268. https://doi.org/10.1128/AAC.04011-14
  7. Choi MJ, Lee EM, Lee SJ, Reza MA, Lee JS, Gebru E, Rhee MH, Park SC. 2011. The in vitro antibacterial activity of florfenicol in combination with amoxicillin or cefuroxime against pathogenic bacteria of animal origin. Pak Vet J 31: 141-144.
  8. Clinical and Laboratory Standards Institute (CLSI). 2008. Performance standard for antimicrobial disk and dilution susceptibility tests for bacterial isolated from animals; Approved standard. CLSI document M31-A3. 3rd ed. Wayne, Pennsylvania, 2008.
  9. Ericsson UH, Lindberg A, Persson WK, Ekman T, Artursson K, Nilsson-Ost M, Bengtsson B. 2009. Microbial aetiology of acute clinical mastitis and agent-specific risk factors. Vet Microbiol 137: 90-97. https://doi.org/10.1016/j.vetmic.2008.12.005
  10. Idriss SE, Foltys V, Tancin V, Kirchnerova K, Tancinova D, Zaujec K. 2014. Mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Nitra, Slovakia. Slovak J Anim Sci 47: 33-38.
  11. Islam NN, Farzana Z, Msudul Azad Chowdhury AM, Mannan A, Kamaruddin KM, Zonaed Siddiki AMAM, Uddin I. 2014. haracterization of bovine subclinical mastitis caused by Staphylococcus aureus in southern Bangladesh by bacteriological and molecular approaches. Asian J Biol Sci 7: 1-12. https://doi.org/10.3923/ajbs.2014.1.12
  12. Kibebew K. 2017. Bovine mastitis: A review of causes and epidemiological point of view. J Biol Agric Healthc 7: 1-14.
  13. Klimiene I, Virgailis M, Pavilonis A, Siugzdiniene R, Mockeliunas R, Ruzauskas M. 2016. Phenotypical and genotypical antimicrobial resistance of coagulase-negative staphylococci isolated from cow mastitis. Pol J Vet Sci 19: 639-646. https://doi.org/10.1515/pjvs-2016-0080
  14. Kumar R, Yadav BR, Singh RS. 2011. Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. J Bio Sci 36: 175-188.
  15. Lee ES, Kang HM, Chung C, Moon JS. 2007. Antimicrobial susceptibility and prevalence of gram-negative bacteria isolated from bovine mastitis. Korean J Vet Res 47: 67-75.
  16. Lim JH, Hwang YH, Park BK, Yun H. 2003. Combination effects of cephalexin and gentamicin on Edwardsiella tarda and Streptococcus iniae. Int J Antimicrob Agents 22: 67-69. https://doi.org/10.1016/S0924-8579(03)00086-4
  17. Moser A, Stephan R, Ziegler D, Johler S. 2013. Species distribution and resistance profiles of coagulase-negative staphylococci isolated from bovine mastitis in Switzerland. Schweiz Arch Tierheilkd 155: 333-338. https://doi.org/10.1024/0036-7281/a000468
  18. Nam HM, Kim JM, Lim SK, Jang KC, Jung SC. 2010. Infectious aetiologies of mastitis on Korean dairy farms during 2008. Res Vet Sci 88: 372-374. https://doi.org/10.1016/j.rvsc.2009.12.008
  19. Nam HM, Lim SK, Jang GC, Joung DY, Kim H, Lee CS, Jung SC. 2013. Culture results from quarter milk samples submitted to veterinary diagnostic laboratories during January-November 2012 in Korea. J Prev Vet Med 37: 111-119. https://doi.org/10.13041/jpvm.2013.37.3.111
  20. Nam HM. 2010. Current situation of mastitis and relative frequency of pathogens isolated from subclinical mastitis in dairy cattle in Korea. Kor J Vet Publ Hlth 34: 265-272.
  21. Piddock LJ. 1996. Does the use of antimicrobial agents in veterinary medicine and animal husbandry select antibiotic-resistant bacteria that infect man and compromise antimicrobial chemotherapy? J Antimicrob Chemother 38: 1-3.
  22. Pillar CM, Goby L, Draghi D, Grover P, Thornsberry C. 2009. Evaluating the in vitro susceptibility of bovine mastitis pathogens to a combination of kanamycin and cefalexin: Recommendations for a disk diffusion test. J Dairy Sci 92: 6217-6227. https://doi.org/10.3168/jds.2009-2282
  23. Rajala-Schultz PJ, Torres AH, Degraves FJ, Gebreyes WA, Patchanee P. 2009. Antimicrobial resistance and genotypic characterization of coagulase-negative staphylococci over the dry period. Vet Microbiol 134: 55-64. https://doi.org/10.1016/j.vetmic.2008.09.008
  24. Rysanek D, Zouharova M, Babak V. 2009. Monitoring major mastitis pathogens at the population level based on examination of bulk tank milk samples. J Dairy Res 76: 117-123. https://doi.org/10.1017/S0022029908003816
  25. Taponen S, Nykasenoja S, Pohjanvirta T, Pitkala A, Pyorala S. 2016. Species distribution and in vitro antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitic milk. Acta Vet Scand 58: 12.
  26. Thomas V, de Jong A, Moyaert H, Simjee S, El Garch F, Morrissey I, Marion H, Valle M. 2015. Antimicrobial susceptibility monitoring of mastitis pathogens isolated from acute cases of clinical mastitis in dairy cows across Europe: VetPath results. Int J Antimicrob Agents 46: 13-20. https://doi.org/10.1016/j.ijantimicag.2015.03.013
  27. Unal N, Askar S, Macun HC, Sakarya F, Altun B, Yildirim M. 2012. Panton-Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants. Trop Anim Health Prod 44: 573-579. https://doi.org/10.1007/s11250-011-9937-7
  28. Yang X, Ouyang W, Li X, Zhang W, Liu Y. 2009. Combination antibacterial effect of compound amoxicillin and levofloxacin hydrochloride nanoemulsion against three main pathogenic bacteria of cow mastitis in vitro. J Zhejiang Univ - Agric Life Sci 35: 585-590.