• Explosives and Blasting
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• v.28 no.2
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• pp.69-75
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• 2010

Excavations by blasting in urban area have caused lots of complaints. Hence, special attentions need to be paid to controlling the ground vibrations in designing blasting for those areas. In this study, among the various parameters that can affect the propagation characteristics of ground vibrations, the effect of the priming location of explosive on the ground vibration level was studied for two types of emulsion explosives that had different detonation velocities. Three priming locations of top, middle, and bottom were considered in a charged hole. In the experiment on the effect of detonation velocity, the ground vibration caused by the explosive with a lower detonation velocity showed larger attenuation in the amplitude. The priming locations also affected the ground vibrations levels. The ground vibration level produced from middle priming was found to be larger than the other priming methods under the same blast conditions, but the attenuation of amplitude was also larger in this case. In contrast, the ground vibration level from bottom priming was not larger than the middle priming, but the attenuation was smaller so that the ground vibration was detected at a longer distance.

• Explosives and Blasting
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• v.29 no.2
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• pp.51-58
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• 2011

Frequency is a very important factor in discussing the effect on facilities such as precision instruments and therefore, in evaluating the effect of blasting vibration, it is necessary to identity information on frequency in addition to maximum amplitude of vibration. This study collected rock samples in gneiss area to perform an indoor rock test and to identify frequency of blasting vibration according to priming location, performed of single hole test blasting. Then the study decided dominant frequency through FFT and analysed changes according to priming locations. Consequently frequency range according to priming location is indicated top priming is distributed high range, bottom priming is distributed high range, middle priming is distributed evenly range. Frequency trend according to priming location is indicated distance increase with frequency discrease in top priming, distance increase with frequency increase in bottom priming.

• Tunnel and Underground Space
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• v.24 no.1
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• pp.97-109
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• 2014

In order to identify the characteristics of the propagation depending on delay time (20, 25 ms) and priming location (top priming, middle priming, bottom priming), test blasts were carried out a total of 4 times using different spacing, burden, drilling length, charge per delay and was derived the formula to predict blast vibration. This study investigated the characteristics of vibration by analysis of the nomogram and prediction of Peak Particle Velocity (PPV) from delay time and priming location by the formula to predict ground vibration. And it analyzed the trends of vibration increase by standards charge 0.5, 1.6, 5, 15 kg. Standards charge is "Blasting design and construction guidelines to road construction" by the Ministry of Land, Infrastructure and Transport. Depending on the charge in favor of vibration control method is proposed. Thus, when the design was to be used as a variable.

• Journal of Industrial Technology
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• v.18
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• pp.385-397
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• 1998

The firing system for the detonators called ordinary blasting caps have almost completely been substituted by safer and more trust worthy systems that can be classified in two groups ; Electric systems, and Non-electric systems. The characteristics of the different initiation devices for both group will be discussed, along with other useful elements for the correct execution of blastings. These detonators are commercialized in several countries under different names such as HiNEL, Nonel, Anodet, Detaline etc. A great advantage is that they do not initiate blasting agents such as slurries and ANFO, allowing bottom priming to be carried out.

• Journal of the Korean Society of Tobacco Science
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• v.18 no.1
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• pp.5-11
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• 1996

Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.