• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.302-306
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• 2005

This Study was Conducted in 3 glasshouse to examine the Possibility for cultivation of plug seedlings using four growing media containing recycled PET-bottle-particles (PBT) as a medium component. Plug seedlings of 'Seokwang' tomato were grown in media containing $100\%$ PBT, $100\%$ peatmoss, or $50\%$ $PBT+50\%$ peatmoss. A commercial plug medium (Tosilee) was used as the control. All seeds were sown in 128 cell plug trays on July 12, 2004. Seedling growth was measured at 30 days after sowing. Percent seed germination was greater than 95% in all treatments. Plant height, shoot fresh and dry weights, leaf area, and hypocotyl length were the greatest in the control treatment. Root length and weight were the greatest in the $100\%$ PBT treatment, which had a medium pH of 7.3. Leaf area and shoot dry weight were greater in the $50\%$ $PBT+50\%$ peatmoss treatment, than in the $100\%$ PBT treatment, while they were the smallest in the $100\%$ peatmoss treatment. No toxicity symptoms caused by the use or PBT had been observed in any treatment. The results suggest a possibility of recycled PBT, a valuable reusable resource, to be used as a component for medium amendments in horticultural transplant production.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.288-293
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• 2010

'Cham', a new cultivar of Agrocybe aegerita, was bred by crossing between monokaryotic strains isolated from GMAG45021 and 'Beodeulsongi1ho' in Mushroom Research Station, Gyonggi Province A.R.E.S. in 2007. The optimum temperature for the mycelial growth of 'Cham' was 26 to $28^{\circ}C$ on PDA medium, whereas that of 'Beodeulsongi1ho'(control) was 24 to $26^{\circ}C$. The optimum temperature for the primordial formation and fruiting body development of 'Cham', as well as 'Beodeulsongi1ho', was 18 to $20^{\circ}C$. In the bottle cultivation, the spawn run period of 'Cham' at 22 to $23^{\circ}C$ was around 38 days and the period from scratching of inoculum to harvest was 12 days. These characteristics of 'Cham' were not different from those of 'Beodeulsongi1ho'. However, 'Cham' had more dark brown-colored, and thicker and stronger pileus, and longer and thicker stipe compared to that of 'Beodeulsongilho'. Freshness of 'Cham' was maintained for 10 days at the storage temperature of $4^{\circ}C$, while that of 'Beodeulsongi1ho' was maintained for 8 days. Moreover, the yield of fruiting bodies of 'Cham' was $141g/850m{\ell}$ bottle, which was similar to that of 'Beodeulsongi1ho'. Both 'Cham' and 'Beodeulsongilho' have lower resistivity against Trichoderma virens and Trichoderma harzianum.

• Journal of the Korean Wood Science and Technology
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• v.26 no.1
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.

• Journal of Mushroom
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• v.15 no.2
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• pp.73-77
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• 2017

In Korea, oyster mushroom is one of the commonly cultivated mushrooms. In 2013, the cultivation areas and products of oyster mushrooms were 60,039 M/T and 201 ha, respectively. Many species of oyster mushrooms are cultivated in various countries. These include Pleurotus ostreatus, P. florida, P. sajor-caju, P. eryngii, P. citrinopileatus, P. salmoneostramineus and P. cystidiosus. P. citrinopileatus is a yellow oyster mushroom famous for its health benefits such as anti-cancer and anti-oxidant effects. Therefore, a cultivar P. citrinopileatus 'Jangdari' was developed to improve yield and the ability to grow well at lower temperatures. Two parent strains 'Gumbit (KMCC02150)' and 'KMCC02145' were selected based on their morphological characteristics. 'Jangdari' was developed by the method of Mon-Mon crossing between monokaryons derived from 'Gumbit' and 'KMCC02145', and finally selected through continuous cultivation tests. The optimum temperature for mycelial growth was $30^{\circ}C$. The cultivar could grow well at high temperatures, especially $16{\sim}24^{\circ}C$. Fruiting body production per bottle (850 mL) was about 90.0 g. Stipe length and thickness of 'Jangdari' were similar to those of 'Gumbit'. 'Jangdari' was more resistant to low temperature than 'Gumbit', and thus it could be cultivated with oyster mushrooms (P. ostreatus). In addition, while cultivating 'Jangdari', it is not required to scrape out the upper side of bottle's sawdust medium; hence, its cultivation is expected to save energy and time.

• Journal of Mushroom
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• v.16 no.1
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• pp.1-8
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• 2018

For the bottle cultivation of pleurotus nebrodensis, poplar tree sawdust was a better substrate than corncobs in terms of primordia and growth characteristics. The nutrition rich substrate was cottonseed dregs, cottonseed coat, bran, corn powder, and rice bran. The mixing ratio was adjusted such that the nitrogen content was ${\leq}58%$; cotton seed dregs, at the level of 20%, were particularly necessary to adjust the nitrogen content for the substrates that had high total nitrogen content. Additionally, inclusion of calcium carbonate in the substrate was better for primordia and growth characteristics than inclusion of calcium hydroxide. In the absence of calcium carbonate, the elasticity of pileus and stipe decreased and their hardness increased. Therefore, the optimum substrate composition for the cultivation of Pleurotus nebrodensis was 40:20:20:15:3:2 mixing ratio of poplar tree sawdust, cottonseed dregs, cottonseed coat, bran, corn powder, and calcium carbonate.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.150-154
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• 2016

To determine a favorable substrate formulation for Grifola frondosa, physicochemical conditions, culture properties, and yields according to various substrate formulations were investigated. Based on these analyses, T4 (80:5:15 ratio of oak sawdust to dried bean-curd refuse to corn husk) resulted in a shorter cultivation period and higher yields (weight of fresh mushrooms harvested at maturity) than those of other treatments. The physicochemical properties of T4 were pH 5.4, 2.4% crude fat contents, 54 C/N ratio, 74.3% porosity, and 0.26 g/cm3 bulk density. These results emphasize the importance of optimal substrate development on the production efficiency of G. frondosa mushrooms and have implications for commercial applications.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.347-350
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• 2003

Pleurotus eryngii is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of Eryngii is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. At the commercial mushroom cultivation houses, this study was carried out to find out reasonable range of each environmental factor and yield together with economic and safe structures influencing on the optimal productivity of Eryngii. This experiment was conducted from Jan. 26, 2003 to Aug. 2, 2003. Ambient temperature during the experiment period was not predominantly different from that of a normal year. The capacity of the hot water boiler and the piping systems were not enough. Maximum temperature difference between the upper and lower growth stage during a heating time zone was about $6^{\circ}C$. The max. and min. relative humidity were ranged approximately $42{\sim}100%$. The $CO_2$ concentration and the illuminance were lowly maintained during growing period. The average yield per bottle was about $54{\sim}102g$.

• Journal of Mushroom
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• v.20 no.2
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• pp.61-68
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• 2022

We investigated the standard cultivation substrate for Sparassis latifolia "Neowul" bred in Jeollabuk-do Agricultural Research and Extension Services. Cultivation characteristics and yield were assessed by varying the kind of sawdust and additives, and the mixing ratio. The cultivation period in larch sawdust was the shortest at 97 days. The yield was excellent (143.6 g). The findings indicated that larch is a tree species appropriate for the cultivation of S. latifolia. When the additives were varied, the yield and productivity (53.1%) were highest (116.6 g) for the wheat bran treatment. Thus, wheat bran would be an additive appropriate for culturing S. latifolia. Concerning the use of different mixing ratios, larch sawdust:beet-pulp:wheat bran ratios of 80:15:5 and 85:10:5 resulted in yields of 114.4 g and 111.4 g, and productivity of 52.5% and 51.8%, respectively. These yield and productivity values were not statistically different. Thus, the standard cultivation substrate for S. latifolia can comprise larch sawdust, beet pulp, and wheat bran at a ratio of 80:15:5 or 85:10:5. The carbon/nitrogen (C/N) ratio assumed to be appropriate for the cultivation of S. latifolia was 184-223. Pinheading would be difficult below a C/N substrate ratio of 105. Thus, the C/N ratio of the media, as well as the pH, would be vital factors affecting pinheading during S. latifolia cultivation.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.58-62
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• 2008

'Miso', a new variety of oyster mushroom was developed for the bottle culture at the Chungnam Agricultural Research & Extension Services. Its mycelium grew rapidly with $8.4{\sim}8.6\;mm/day $ at $25{\sim}28^{\circ}C$ on PDA medium. The optimum pH of the mycelial growth was pH 5.0. It took 24 days for the primitive primordium formation after inoculation on pine sawdust media mixed with 20% wheat bran. Fruiting body color was white, and the shape of pileus was convex-umbonate. In the bottle culture, the yield was 115.7 g per 850 ml bottle. Stipe length was about 54 mm, the number of stipe per bottle was 18.1, the size of pileus was 28 mm, and gill was crowded. The moisture content of 'Miso' was lower than that of Jangan No.5 and Wonhyeong No.1, and contents of protein, ash and sugar of 'Miso' were higher than those of other varieties, and tannin acid content of 'Miso' was lower than that of others. On the basis of AFLP analysis, the 'Miso' was distinct not only from Wonhyeong No.1, but also from their closest relative, oyster mushrooms.

• Journal of Mushroom
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• v.10 no.1
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• pp.15-20
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• 2012

Ventilation effects of bottles($1,100m{\ell}$) for culturing Flammulina velutipes on its mycelial growth and mushroom production were investigated. The degree of ventilation were controlled with hole positions, upper and under, and hole sizes in the bottle lids. The ventilation effects were measured with the contents of carbon dioxide, free sugars, chitin, moisture in the bottles and with the amount of produced mushrooms from the bottles. Carbon dioxide concentrations within the culturing bottles at exponential mycelial growth period vertex were relatively high in the bottles with lids without both a sponge and an aeration hole, and in those with a smaller hole. Free sugar contents in the mycelia were the highest in those with a 47mm hole on both sides, and in those with 26~33mm holes only underside. Chitin content was the highest in those with a 26mm hole only underside. On the other hand, the lids with 42mm~47mm holes on the both sides greatly lost water and decreased the mushroom production. In conclusion, the most efficient ventilation hole sizes on the lids for bottle($1,100m{\ell}$) cultivation of Flammulina velutipes using $1,100m{\ell}$ polypropylene bottle were 19mm on both sides of the lid and 26mm on only underside. They produced more mushrooms than the control by 6~9 %.