• Journal of Mushroom
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• v.10 no.2
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• pp.68-73
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• 2012

The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture and yield were observed in Pleurotus ostreatus 1,100ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 6~11days cultivation irrespective of cap sizes and types. The upper-under 19~38mm perforation hole and under 26~47mm perforation hole of caps in the 1,100ml bottle were best condition for cultivation of mushroom and increased fruit body, 11.4~23.8% and 6.5~17.9%, respectively. However, the upper-under 33mm perforation hole of fruit body were decreased 23.8%. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.

• Journal of Mushroom
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• v.13 no.2
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• pp.139-144
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• 2015

This study was done the calculation formula for calculating the below item. That is the moisture content of the substrates raw materials, water content of the mixed substrates; the amount of media, dry weight, amount of liquid, bulk density in the bottle; dried materials volume, water volume, mixing ratio in a substrates mixing materials; water requirement amount, replenishment amount for water content control of the substrates and the total amount of substrates. And each was prepared in an Excel file for automatically calculated. The calculation of the Excel file will be able to take advantage for the mushroom growers; to make raw material costs, per bottle nutrient content and moisture content maintained throughout the year and new media composition. At this time we expect, while minimizing the quantity or deterioration in the daily ups and downs continued growing courses throughout the year contribute to stabilizing production.

• Journal of Mushroom
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• v.7 no.4
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• pp.156-162
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• 2009

These experiments were carried out to determine the optimal culture conditions for nine strains of collected Hatakeshimeji, Lyophyllum decastes (Fr.:Fr.) Sing. SPA 202 and SPA 205 strains were selected because mycelium grew fast and showed fine density. All strains showed fast mycelial growth and mycelial density on BC(Burke compost) media for 20 days of incubation. The optimal sawdust species for the mycelial growth were the fermented sawdusts of Quercus aliena and Populus deltoides. Spawn running period on the fermented sawdust substrate required 50 days at 20 to $25^{\circ}C$ and additional 7 days after soil casing. Cultivation period and temperature forprimordia formation and fruitbody development appeared from 10 to 11 days and from 7 to 8 days at 17 to $18^{\circ}C$ respectively. The length of pilei and stipes of SPA 202 harvested in optimal stage showed 60mm and 67mm, respectively. Yield of SPA 202 strain grown on fermented sawdust substrate was 130g per 1,100ml in bottle cultivation. The length of pilei and stipes of SPA 205harvested in optimal stage showed 51mm and 81mm, respectively. Yield of SPA 205 strain grown on fermented sawdust substrate was 129g per 1,100 ml in bottle cultivation. SPA 202 strain and SPA 205 strain in artificial bottle cultivation of Lyophyllum decastes used in fermented sawdust substrate were selected as themost appropriate strain in yield.

• Journal of Mushroom
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• v.18 no.4
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• pp.303-310
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• 2020

The cultivation of Sparassis crispa in the beginning of the 2000s in South Korea, and the cultivar 'Neowul' bred in the Chonbuk Agricultural Research and Extension Service were registered first in 2016. However, there is no manual for the cultivation of Sparassis crispa, and therefore, there remains a big difference in its harvest rate across farms. Herein, we aimed to study the primordium formation conditions of Sparassis crispa 'Neowul' according to the medium pH, medium moisture content, and inoculum volume of liquid spawn and develop a stable production technology. We found the annual yield per bottle relating to the cultivation period, harvest rate, and the weight of fruiting body to be the highest at 363.6 g in the area cultivated at pH 3.8. However, it is thought that cultivation by adjusting the pH to 3.9±1 would be necessary for stable production, considering that at pH 3.6, the yield sharply reduced to 189.5 g. Moreover, the culture period was shorter at pH 4.0 compared with that at pH3.8, and the cultivation period at pH 4.0 was the same as that at pH 3.8. No significant difference in the weight of the fruiting body at different conditions was recognized. Additionally, it is difficult to regulate the pH precisely in practical applications in the farms. It is thought that 341.8 g Sparassis crispa will be produced per bottle annually if the medium moisture content is adjusted to 65%, liquid spawn inoculum volume is equivalent to 4% of the medium volume, and the humidity in the culture room is set to below 50%.

• Korean Journal Plant Pathology
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• v.10 no.3
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• pp.175-180
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• 1994

The fungal pathogen Monosporascus annonballus was first isolated in Korea from the rotted roots of bottle gourd stocks of collapsed watermelon plants in fields near Chochiwon, Choongnam province in July, 1993. Perithecia of M. cannonballus were dark brown to black, globose, 220~570 ${\mu}{\textrm}{m}$ in diam. and had many asci. Asci are hyaline, clavate to pyriform, and 50~120$\times$35~570 ${\mu}{\textrm}{m}$ in size. Ascospores were aseptate, dark brown to black, globose, 25~45$\times$30~50 ${\mu}{\textrm}{m}$ in diam, and borne singly in each ascus. The fungus grew in the temperature range of 4 to 34$^{\circ}C$, best at 3$0^{\circ}C$. The optimum pH for growth was 6.8. Mycelial growth rate of M. cannonballus was 25.5mm/day on PDA at 26$^{\circ}C$. Perithecia began to form after 20-day-growth on PDA and produced mature asci after 30 days or later. In the greenhouse inoculation tests, the fungus developed water-soaked lesions on roots of bottle gourd seedlings and was then reisolated from the lesions. Severed damages on watermelon plants by M. cannonballus are greatly concerned in Korea, since no stocks used for watermelon cultivation have reported to be resistant to the fungus.

• Journal of Mushroom
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• v.19 no.2
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• pp.88-95
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• 2021

This study was carried out to breed new variety of Pleurotus nebrodensis. We have collected and tested characteristics of genetic resources from domestic and abroad since 2015. The varieties of P. nebrodensis from China are grown by farmers, but those have been unstable fruiting and are weak against bacterial diseases. To solve this problem, we bred the unique domestic variety 'Uram' of P. nebrodensis and the results of the characteristic test for the new 'Uram' are as follows. The proper temperature for the mycelial growth was 26~29℃ and fruit body growth temperature was 15~18℃. It was similar to the control variety KME65035 of P. nebrodensis in the pileus form of a flat and white color. The number of days required for initial fruting was 5 days for bottle cultivation and 6 days for bag cultivation which was 2-4 days shorter than that of the control variety. The pileus diameter was 32.6-37.0 mm which was smaller but the fruit body length was 130.4 mm, which was longer than those of the control variety. The effective number of fruit bodies was 1.8 in bottle cultivation and 2.9 in bag cultivation, which was more than those of the control variety. The yield rate was 93.3-100%, which was more stable than those of the control variety. In bottle cultivation and bag cultivation, the yield was 173.1 g/bottle (1100 cc) and 283.4 g/bag (1.2 kg), respectively, which was 25-44% higher than those of the control variety 138.0 g/bottle (1100 cc) and 197.4 g bag (1.2 kg). When incubating the parent and control varieties of 'Uram', the replacement line was clear and as a result of mycelial DNA RAPD-PCR reaction, the band pattern was different from that of the parent and control varieties, confirming the hybrid species.

• Journal of Mushroom
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• v.13 no.2
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• pp.92-96
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• 2015

'Geumhyang', a new variety with short cultivation period and yellowish ivory color in Flammulina velutipes, was bred by crossing between monokaryons isolated from 'Garlmoe' and wild strain 'CBMFV-33'. In bottle cultivation, the period of mycelial growth, primordia1 formation, and growth of fruit body was required 23 days, 7 days, and 17 days, respectively. Total culvivation period was 47 days, showing 7 days shorter than that of 'Garlmoe'. Pileus diameter was greater than that of 'Garlmoe', and pileus color showed yellowish ivory. Fruit body production per 850 ml bottle was 160 g, which was 8% higher than that of 'Garlmoe'. The taste, derived from 'Garlmoe' and wild strain, was excellent because of better chewing feeling than white ones. 'Geumhyang' with differentiated color and taste, has an advantage to target a niche market of white ones.

• Journal of Mushroom
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• v.3 no.3
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• pp.105-108
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• 2005

Main materials used as media for oyster mushroom cultivation are pine sawdust, beet pulp, cotton seed pomace. Increases in the price and the unbalance of demand and supply of cotton seed pomace was often damage to oyster mushroom cultivation farm, so we investigated agricultural by-product to replace the cotton seed pomace for bottle cultivation of oyster mushroom. Treatment of coconut oil meal or coconut pomace delayed incubation period about 3 days compared with cotton seed waste treatment(control), but yield and income index showed similar to each other in three treatment. Consequently coconut oil meal and coconut pomace could select for cotton seed pomace substitute.

• The Korean Journal of Mycology
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• v.31 no.2
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• pp.94-97
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• 2003

In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.67-78
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• 2007

This study was carried out to monitor feed-nutritional components, toxic heavy metals (Cd, Pb and As) and pesticide residues through three cultivation stages (1st initial culture stage, 2nd mycelial growth stage, and 3rd fruit body-harvested stage) of king oyster mushroom (Pleurotus eryngii) produced by bottle type cultivation and oyster mushroom (Pleurotus osteratus) produced by vinyl bag type cultivation. For both cultivation types, compared with the initial culture, the weight reduction rate in spent mushroom substrates (SMS) after fruit body harvest was 29% for total wet mass, 21～25% for dry and organic matters and 19 ～22% for neutral detergent fiber. Two thirds to 3/4 of organic matter degraded and utilized by mycelia and fruit bodies was originated from fiber, of which the primary source (50～70%) was hemicellulose. The effect of mycelial growth stage on chemical compositional change in culture was little (P>0.05) for bottle type cultivation of king oyster mushroom but considerable (P<0.05) for vinyl type cultivation of oyster mushroom. Culture nutrients uptake by fruit bodies was very active for the bottle type cultivation. Compared with SMS, harvested fruit bodies (mushrooms) contained higher (P<0.05) crude protein, non-fibrous carbohydrate, and crude ash and lower (P<0.05) neutral detergent fiber. Regardless of stages, no culture samples were contaminated with toxic heavy metals and pesticide residues. In conclusion, the increase of fiber (neutral and acid detergent fibers) and indigestible protein contents and the decrease of true protein content in SMS indicated that the feed-nutritional value of SMS was significantly reduced compared with that of the initial culture and they were safe from toxic heavy metals and pesticide residues.