• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.228-235
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• 2006

Pseudomonas aeruginosa $GRC_2$, siderophore-producing strain, inhibited growth of Macrophomina phaseolina in vitro and reduced charcoal rot in seeds of Brassica juncea in field when coated with adaptive strains. P. aeruginosa $GRC_2$ and Azotobacter chroococcum $AC_1$ produced indole-3-acetic acid and solubilized insoluble phosphate. A. chroococcum $AC_1$ fixed nitrogen asymbiotically. Urea and diammonium phosphateadaptive variant strains of P. aeruginosa and A. chroococcum strongly inhibited M. phaseolina in comparison to parental strains. Bacterization of seeds induced seed germination, seedling growth, and enhanced yield of B. juncea by 10.87% as compared to full doses of urea and diammonium phosphate. Both adaptive strains of chemical fertilizers aggressively colonized roots, showing effectiveness to growth and developments of B. juncea.

• Mycobiology
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• v.34 no.1
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• pp.22-29
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• 2006

Forty-one fungal species belonging to 15 fungal genera isolated from Egyptian soil and sugar cane waste samples were tested for their capacity of producing acidity and gluconic acid. For the tests, the fungi were grown on glucose substrate and culture filtrates were examined using paper chromatography analysis. Most of the tested fungi have a relative wide potentiality for total acid production in their filtrates. Nearly 51% of them showed their ability of producing gluconic acid. Aspergillus niger was distinguishable from other species by its capacity to produce substantial amounts of gluconic acid when it was cultivated on a selective medium. The optimized cultural conditions for gluconic acid yields were using submerged culture at $30^{\circ}C$ at initial pH 6.0 for 7 days of incubation. Among the various concentrations of substrate used, glucose (14%, w/v) was found to be the most suitable carbon source for maximal gluconic acid during fermentation. Maximum values of fungal biomass (10.02 g/l) and gluconic acid (58.46 g/l) were obtained when the fungus was grown with 1% peptone as sole nitrogen source. Influence of the concentration of some inorganic salts as well as the rate of aeration on the gluconic acid and biomass production is also described.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.133-139
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• 1993

Common dermatophytosis in upper Egypt were studied at three hospitals in Minia city. Tinea corporis (ringworm of glabrous skin) was diagnosed in 127 patients (84 males and 53 females). Trichophyton was the most common genus and was represented by 6 species among which T. violaceum was the commonest fungus. Microsporum was represented by 4 species and M. canis was the prevalent species. Tinea pedis (ringworm of the foot) was noticed in 22 cases (13 males and 9 females) and also Trichophyton and Microsporum were the causal organisms. Three species of Trichophyton namely: T. violaceum, T. kuryngei and Trichophyton sp. were isolated. Tinea cruis (ringworm of the groin) was diagnosed in 21 patients (16 males and 5 females). Epidermophyton floccosum was the most prevalent fungus associated with tinea cruris whereas Trichophyton and Candida were less frequent. Tinea unguium (ringworm of the nail) was diagnosed in 9 patients, 8 were females and one was male and the age incidence ranged between 5 and 55 years. M. canis occurred in 2 cases and T. mentagrophytes in one case. Out of 86 cases of tinea versicolor, 71 were males and 15 were females. Malassezia furfur was the main causal agent. All patients of candidiasis and paronychia were females and Candida was the most prevalent causal agent.

• Mycobiology
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• v.32 no.3
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• pp.128-133
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• 2004

Twenty plant extracts were tested against mycelial growth, sclerotium formatiom and dry weight of mycelium with sclerotia of Sclerotium rolfsii Sacc. The highest(90 mm) mycelial growth was measured in Adhatoda vasica, Tegetes erecta, Allium cepa, and Curcuma longa. The lowest(25 mm) was in Azadirachta indica. No mycelial growth was found in any concentration of cow, buffalo, and goat urine. The highest(90 mm) and the lowest(15 mm) mycelial growth were measured in Biomil and Urea, respectively. No mycelial growth was observed in Zinc. The highest(60 mm) and the lowest(2 mm) mycelial growth were recorded in Macuprex(Dodine; 65% WP) and Boron(100% Boric acid and 17% Boron) respectively. Mycelial growth was totally inhibited in Rovral(Iprodione; 50% WP).

• Journal of Forest and Environmental Science
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• v.27 no.1
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• pp.1-15
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• 2011

The present study was undertaken in middle altitudinal (1500 to 2500 masl) moist temperate forest of Mandal-Chopta area in the Garhwal region of Uttarakhand, India. The aim of the present study was to assess the variation in species composition and diversity in different vegetation layers viz. herb, shrub and tree, at different altitudes. Shannon-Wiener diversity index ($\bar{H}$), $Nha^{-1}$, total basal cover per hectare (G), Simpson concentration of dominance, Pielou Equitability, species richness (SR), Margalef index, Menheink index of species richness and ${\beta}$-diversity were calculated to understand community composition. Tree G ranged from 84.25 to 35.08 $m^2ha^{-1}$ and total stem density varied from 990 to 1470 Nha-1. Total SR (herb, shrub and trees) among different forest types ranged between 31 and 58. Maximum G of herb and shrub layers was recorded at lower altitudes between 1500 and 1650 masl. ${\beta}$-diversity was higher in herb layers as compared to tree and shrub layers. Dominance-diversity curves were also drawn to ascertain resource apportionment among various species in different forest types. Values of species diversity, $\bar{H}$, $Nha^{-1}$ and G were higher in the study area as compared to similar forests growing in other parts of Uttarakhand Himalaya.

• Journal of Forest and Environmental Science
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• v.35 no.1
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• pp.54-60
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• 2019

Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenlandia umbellata. The leaves developed in vitro after $4^{th}$ subcultures of multiplication phase and after 6 weeks of field transferred plants were used. Statistically significant differences in the number of stomata, veins, raphides, crystals and trichome density per square mm were observed. The improvements in stomatal apparatus and density (decreased from 41.85 to 32.20), developments in leaf architectural parameters and emergence of defense mechanism through increased numbers of raphides (8 to 15), crystals and trichomes (13.5 to 18.2) proved acclimation of tissue culture raised plantlets from in vitro to the in vivo environments lead to 100 % success in field establishment of the plantlets. The in vitro induced foliar abnormalities (changes in stomata, venation pattern, vein density, trichomes, crystals etc.) were repaired while hardening of plantlets in the greenhouse and finally in the field. The observed micromorphological response of leaves under altered environmental conditions could help in determination of proper stage of field transfer and prediction of survival percentage of in vitro regenerated O. umbellata plantlets.

• Korean Journal of Plant Taxonomy
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• v.49 no.1
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• pp.13-27
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• 2019

Numerical taxonomy is employed to determine the phenetic proximity of the Egyptian taxa belonging to the genus Ononis L. A classical clustering analysis and a principal component analysis (PCA) were used to separate 57 macro- and micromorphological characters in order to circumscribe 11 taxa of Ononis. A clustering analysis using the unweighted pair-group method with the arithmetic means (UPGMA) method gives the highest co-phenetic correlation. Results from clustering and PCA revealed the segregation of five groups. Our results are in line, to some certain degree, with the traditional sub-sectional concept, as can be seen in the grouping of the representative members of the subsections Diffusae and Mittisimae together and the representative members of the subsections Viscosae and Natrix. The phenetic uniqueness of Ononis variegata and O. reclinata subsp. mollis was formally established. However, our findings contradict the classic sectional concept; this opinion was suggested earlier in previous phylogenetic circumscriptions of the genus. The most useful characters that provide taxonomic clarity were discussed.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.289-297
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• 2020

The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii (L.f.) Cochrane & Iltis. The study justifies the recognition of the two subpopulations of Corynandra chelidonii from aquatic and terrestrial environments as two distinct varieties, C. chelidonii var. pallae (Reddy & Raju) V.S.Raju and C. chelidonii var. chelidonii, respectively, based on the traditional taxonomic evidence.

• Journal of Forest and Environmental Science
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• v.26 no.2
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• pp.117-129
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• 2010

The present study was undertaken in moist temperate forest of Mandal-Chopta area in the Garhwal region of Uttarakhand, India. The aim of the present study was to assess the physical properties of soils in relation to the forest structure and composition. Twelve forest types according to the altitude, slope aspect and species compositions were selected for the study. Physical properties of soil i.e., soil colour, soil texture (per cent of sand, silt and clay), moisture content, water holding capacity, porosity, bulk density (gm/$cm^3$) and void ratio were analyzed for three different depths viz., (i) 'upper' (0-10 cm), (ii) 'middle' (11-30 cm) and (iii) 'lower' (31-60 cm) in all the selected forest types. Phytosociological and diversity parameters viz. total basal cover ($Gha^{-1}$), stem density ($Nha^{-1}$), tree species richness, Simpson concentration of dominance and Shannon-Wiener diversity index were also calculated for each forest type. This study also provides the comparisons between the results of physical analysis of the present study with numerous other previous studies in the temperate Himalayan region of the Uttarakhand.

• Korean Journal of Plant Taxonomy
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• v.44 no.3
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• pp.178-180
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• 2014

We report a new record of Jasminum albicalyx Kobuski in Vietnam. The plant differs from J. pedunculatum Gagnep., a closely related taxon, by foliar and floral characters such as less number of lateral veins, presence of domatia at vein axils on the abaxial surface of leaf, smaller bracts, white calyx having more number of lobes and less number of lobes in corolla. Morphological description, line drawing and color photographs are provided in addition to the species key of allied species of Jasminum albicalyx in Vietnam.