• Title/Summary/Keyword: Botany

Search Result 804, Processing Time 0.021 seconds

Sporocarp development of Pleurotus tuber-regium Fr. under different watering systems

  • Okhuoya, J.A.;Ajerio, C.
    • The Korean Journal of Mycology
    • /
    • v.16 no.4
    • /
    • pp.207-209
    • /
    • 1988
  • Sporocarps of Pleurotus tuber-regium Fr. were subjected to aerial and substrate(soil) watering sytems. Although aerial watering enhanced quick enlargment of pilei, those watered indirectly through the soil developed more naturally pigmented sporophores and with higher overall yield. Thus, substrate(soil) watering after emergence is preferred to direct watering of fruits.

  • PDF

Calcium-Boron Interaction in Exopolysaccharide Production by the Cyanobacterium, Nostoc spongiaeforme

  • Singh;Netu;Asthana, R.K.;Singh, S.P.
    • Journal of Microbiology and Biotechnology
    • /
    • v.10 no.3
    • /
    • pp.381-385
    • /
    • 2000
  • The effect and interaction of Ca and B on exopolysaccharide (EPS) synthesis in the diazotrophically growing cyanobacterium. Nostoc spongiaeforme, was investigated. The absence of B inhibited EPS synthesis 1.56-fold ($16\mu\textrm{g}$ glucose equivalent/mg dry weight, 16 d) over the control cells ($25\mu\textrm{g}$ glucose equivalent) grown in medium containing 0.5 mM Ca and $8{\mu}{\textrm}{m}$ B. When B concentration was raised to $40{\mu}{\textrm}{m}$, EPS production was stimulated 1.8-fold. Reduction of Ca concentraion to one-half (0.25 mM) resulted in increased B demand (16$\muM$) by the cells for EPS production at par with the normal sets. However, without Ca, EPS production also increased as B increased. Addition of B to a Ca-free medium stimulated cyanobacterial diazotrophic growth as well as synthesis of Chl a and phycocyanin (0-8 d). The data suggest B-dependent diazotrophic growth during Ca-deficiency and point to and important interactive role of Ca and B in regulation of cyanobacterial EPS synthesis.

  • PDF

Forest Structure and Composition in the Vicinity of Srinagar Hydroelectric Power Project in Alaknanda Valley, Garhwal Himalaya, India

  • Ballabha, Radha;Kuniyal, Archana;Tiwari, Prabhawati;Tiwari, Jay Krishan
    • Journal of Forest and Environmental Science
    • /
    • v.36 no.2
    • /
    • pp.78-90
    • /
    • 2020
  • We studied forest structure and composition in the vicinity of Srinagar Hydroelectric Power Project in Alaknanda Valley, Garhwal Himalaya, India to provide baseline data for the management of forests. Eight sites were selected in the vicinity of power project based on elevation and species composition. Density varied from 650 to 340 ind ha-1 for trees and from 4,360 to 6,480 ind ha-1 for shrubs. TBC ranged from 35.02 to 54.02 ㎡ ha-1 for trees and from 0.875 to 2.628 ㎡ ha-1 for shrubs. On the basis of density and IVI, Pinus roxburghii was found dominant tree species in most of the sites, whereas among the shrubs, Carissa opaca was dominant. Dominance of Pinus roxburghii in most of the sites is an indication towards possible threat to associated species in the area. The dominance-diversity curve of trees showed a geometric distribution, whereas the shrubs displayed log-normal curves. The forest has rich and diverse species composition however; habitat degradation caused by the construction of Power Project might lead to reduction of plant species from the area. The information obtained from this study will be helpful in predicting possible changes in the forest ecosystem properties in near future after completion of the power project.

Cultural conditions affect somatic embryogenesis in Catharanthus roseus L. (G.) Don

  • Aslam, Junaid;Mujib, A.;Fatima, Samar;Sharma, M.P.
    • Plant Biotechnology Reports
    • /
    • v.2 no.3
    • /
    • pp.179-189
    • /
    • 2008
  • We established an efficient plant regeneration system for Catharanthus roseus L. (G.) Don through somatic embryogenesis. Embryogenic callus was induced from hypocotyl of seed germinated in vitro. Somatic embryogenesis in Catharanthus has been categorized into three distinct stages: (1) initiation and proliferation of embryo; (2) maturation, and; (3) germination or plantlet conversion. Beside plant growth regulators, various stages of embryogenesis were screened for their response to a wide variety of factors (pH, gelrite, light, sugar alcohols, polyethyleneglycol and amino acids), which affect embryogenesis. All of the tested factors had a small to marked influence on embryogeny and eventual conversion to plantlets. The plantlets were acclimatized successfully in a greenhouse. To our knowledge, this is the first report describing a detailed study of various cultural factors which regulate embryogenesis in C. roseus. The results discussed in this paper may be used in mass propagation to produce medicinal raw material, and the embryo precursor cells could be used in genetic modification programmes that aim to improve the alkaloid yield as well.

Plant Inositol Signaling - Biochemical Study of Phospholipase C and D-myo-inositol -1,4,5-trisphosphate receptor

  • Martinec, Jan;Feltl, Tomas;Nokhrina, Katerina;Zazimalova, Eva;Machackova, Ivana
    • Korean Journal of Plant Tissue Culture
    • /
    • v.27 no.5
    • /
    • pp.375-377
    • /
    • 2000
  • It is now generally accepted that a phosphoinositide cycle is involved in the transduction of a variety of signals in plant cells. In animal cells, the hydrolysis of phosphatidyl-4,5-bisphosphate catalysed by phosphatidylinositol - specific phospholipase C yields to D-myo-inositol - 1,4,5-trisphosphate and diacylglycerol, which are well known second messengers. The binding of InsP$_3$to a receptor located on the endoplasmic reticulum triggers a calcium release from the endoplasmic reticulum. We have detected and partially characterised key components of phosphoinositide signaling. First, tobacco microsomal fraction and plasma membrane PI-PLC. Consecutively, using a radioligand binding assay we have identified a $Ca^{2+}$ -dependent high affinity InsP$_3$binding site in microsomal membrane fraction vesicle preparation and then we have measured inositol-1,4,5-trisphosphate induced calcium release from tobacco microsomal fraction. These findings suggest that phosphoinositide signaling system is present and operates in the tobacco suspension culture.e.

  • PDF

Agrobacterium tumefaciens Mediated Genetic Transformation of Pigeonpea [Cajanus cajan (L.) Millsp.]

  • Kumar, S.Manoj;Syamala, D.;Sharma, Kiran K.;Devi, Prathibha
    • Journal of Plant Biotechnology
    • /
    • v.6 no.2
    • /
    • pp.69-75
    • /
    • 2004
  • Optimal protocol for efficient genetic transformation has been defined to aid future strategies of genetic engineering in pigeon pea with agronomically important genes. Transgenic pigeonpea plants were successfully produced through Agrobacterium tumefaciens-mediated genetic transformation method using cotyledonary node explants by employing defined culture media. The explants were co-cultivated with A. tumefaciens strain C-58 harboring the binary plasmid, pCAMBIA-1301 [con-ferring $\beta$-glucuronidase(GUS) activity and resistance to hygromycin] and cultured on selection medium (regeneration medium supplemented with hygromycin) to select putatively transformed shoots. The shoots were then rooted on root induction medium and transferred to pots containing sand and soil mixture in the ratio of 1:1. About 22 putative TO transgenic plants have been produced. Stable expression and integration of the transgenes in the putative transgenics were confirmed by GUS assay, PCR and Southern blot hybridization with a transformation efficiency of over 45%. Stable integration and expression of the marker gene has been confirmed in the TO and T1 transgenics through PCR, and Southern hybridization.

Dermatophyte and Cyclohexamide-Resistant Fungi Isolated from Patients with Tinea Capitis and from Air in Hospitals in Minia, Egypt

  • Moubasher, A. H.;El-Naghy, M. A.;Maghazy, S. M.;El-Gendy, Z.
    • The Korean Journal of Mycology
    • /
    • v.21 no.1
    • /
    • pp.77-84
    • /
    • 1993
  • Out of 210 tinea capitis cases studies, 16 were negative when examined with KOH, among the remaining 194 cases, 123 were males (63.4%) and 71 were females (36.6%) and the age of incidence ranged between 7 and 15 years. Microsporum was the main causal agent being identified in 82 cases (42.3%) and was represented by 5 species among which M. canis was common in Egypt (55 cases, 28.4%). Trichophyton constituted 32% and was represented by 8 species among which T. violaceum was the most common (24.2% of total cases). Candida were isolated from 3.6% of total cases. The 47 species and twenty-five genera from nondermatophyte-cyclohexamide resistant fungi were recovered from the diseased skin tissue. Penicillium and Aspergillus were the most abundant followed by Scopulariopsis, Alternaria, Thermoascus, Chrysosporium and Cladosporium. Studies of the air-borne fungi in-door the hospital wards revealed the occurrence of 57 species belonging to 28 genera, among which Aspergillus flavus, Penicillium chrysogenum, p. corylophilum, A. niger, Tritirachium rosum and Alternaria alternata were the most common. Results of the out-door experiments were basically similar to those of the in-door experiments.

  • PDF