• 제목/요약/키워드: Bone Characteristics

검색결과 941건 처리시간 0.029초

Inhibitory Effect of Curcumin on MDR1 Gene Expression in Patient Leukemic Cells

  • Anuchapreeda, Songyot;Thanarattanakorn, Pattra;Sittipreechacharn, Somjai;Tima, Singkome;Chanarat, Prasit;Limtrakul, Pornngarm
    • Archives of Pharmacal Research
    • /
    • 제29권10호
    • /
    • pp.866-873
    • /
    • 2006
  • When patients with cancers are treated with chemotherapeutic agents a long time, some of the cancer cells develop the multidrug resistance (MDR) phenotype. MDR cancer cells are characterized by the overexpression of multidrug resistance1 (MDR1) gene which encodes P-glycoprotein (Pgp), a surface protein of tumor cells that functions to produce an excessive efflux and thereby an insufficient intracellular concentration of chemotherapeutic agents. A variety of studies have sought potent MDR modulators to decrease MDR1 gene expression in cancer cells. Our previous study has shown that curcumin exhibits characteristics of a MDR modulator in KB-V1 multidrug-resistant cells. The aim of this study was to further investigate the effect of curcumin on MDR1 gene expression in patient leukemic cells. The leukemic cells were collected from 78 childhood leukemia patients admitted at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, in the period from July 2003 to February 2005. There were 61 cases of acute lymphoblastic leukemia (ALL), 14 cases of acute myeloblastic leukemia (AML), and 3 cases of chronic myelocytic leukemia (CML). There were 47 males and 31 females ranging from 1 to 15 years old. Bone marrows were collected. The leukemic cells were separated and cultured in the presence or absence of $10{\mu}M$ curcumin for 48 hours. MDR1 mRNA levels were determined by RT-PCR. It was found that curcumin reduced MDR1 gene expression in the cells from 33 patients (42%). Curcumin affected the MDR1 gene expression in 5 of 11 relapsed cases (45%), 10 of 26 cases of drug maintenance (38%), 7 of 18 cases of completed treatment (39%), and 11 of 23 cases of new patients (48%). The expression levels of MDR1 gene in leukemic patient cells as compared to that of KB-V1 cells were classified as low level (1-20%) in 5 of 20 cases (25%), medium level (21-60%) in 14 of 32 cases (44%), and high level (61-100%) in 14 of 20 cases (70%). In summary, curcumin decreased MDR1 mRNA level in patient leukemic cells, especially in high level of MDR1 gene groups. Thus, curcumin treatment may provide a lead for clinical treatment of leukemia patients in the future.

교정용 미니스크류의 디자인에 따른 식립 및 제거 토오크 (Insertion and removal torques according to orthodontic mini-screw design)

  • 차정열;윤태민;황충주
    • 대한치과교정학회지
    • /
    • 제38권1호
    • /
    • pp.5-12
    • /
    • 2008
  • 교정용 미니스크류의 원추 형태가 식립 후 시기별 안정성에 미치는 영향을 알아보기 위해, 시기별 제거 토오크 및 동요도와 조직시편분석을 시행하였다. 성견 6마리의 하악 협측골에 원통형과 원추형 교정용 미니스크류 (Biomaterials Korea, Seoul, Korea) 48개를 식립하고, 식립 시 식립 토오크와 동요도를 측정하고 3주, 12주 동안 교정력을 부여한 후, 제거 시 제거 토오크와 동요도를 측정하였다. 식립 토오크는 원통형 미니스크류에서 13.6 Ncm, 원추형 미니스크류에서 22.3 Ncm 로 원추형 미니스크류에서 높은 식립 토오크가 측정되었다 (p < 0.001). 식립 3주째 제거 토오크는 원통형 미니스크류에서 5.7 Ncm, 원추형 미니스크류에서 9.1 Ncm 로 유의한 차이가 관찰되었다 (p < 0.05). 식립 12주째 원추형 미니스크류의 제거 토오크는 3.6 Ncm 로 유의하게 감소하여 (p < 0.05) 원통형 미니 스크류의 4.2 Ncm 와 유의한 차이를 보이지 않았다. 이상의 결과로 미니스크류의 원추 구조는 식립 토오크를 유의하게 증가시켜 식립 후 3주간 안정성에 도움이 되었으나, 12주 후 안정성에 있어서는 원통형 미니스크류와 유사하였음을 시사한다.

붕장어 Frame을 이용한 스낵의 제조 및 특성 (Preparation and Characteristics of Snack Using Conger Eel Frame)

  • 김혜숙;강경태;한병욱;김은정;허민수;김진수
    • 한국식품영양과학회지
    • /
    • 제35권10호
    • /
    • pp.1467-1474
    • /
    • 2006
  • 수산가공 부산물인 fish frame을 보다 효율적으로 이용하기 위하여 붕장어 frame을 주성분으로 하는 스낵의 제조를 시도하였고, 아울러 이의 특성도 검토하였다. 스낵의 제조 원료로 검토한 붕장어 frame의 경우 휘발성염기질소 함량으로 미루어 보아 가공 원료로 적절하리라 판단되었다. 관능검사의 결과와 단가 측면에서 붕장어 frame스낵의 감미제로는 물엿이 적절하리라 판단되었다. 수분함량 및 과산화물값의 결과로 미루어 보아 붕장어 frame을 이용한 물엿 함유 조미액 처리 스낵의 경우 식품위생법규 상으로 적절한 식품이었다. 붕장어 frame을 이용한 물엿 함유 조미액 처리 스낵은 대두유와 유사한 지방산 조성을 가졌으나 EPA 및 DHA의 조성비도 무시할 수 없는 정도이었다. 붕장어 frame을 이용한 물엿 함유 조미액 처리 스낵의 구성아미노산 총 함량은 23.9%이었고 주요 아미노산으로는 aspartic acid, glutamic acid, glycine 및 alanine 등이었다. 또한 붕장어 frame을 이용한 물엿 함유 조미액 처리 스낵의 칼슘 및 인의 함량은 각각 4.9% 및 2.7%이었고, 칼슘/인의 비율은 1.82로 칼슘 흡수가 기대되는 비율이었다. 붕장어 frame을 이 용한 물엿 첨가 스낵의 EPA 및 DHA 등과 같은 건강 기능성 지방산의 조성, 구성아미노산의 함량 및 칼슘과 인의 함량 등은 시판 뱀장어 frame 스낵에 비하여 우수하였다.

하악이부와 두개안면골격의 상관성에 관한 측모두부방사선 계측학적 연구 (A CEPHALOMETRIC STUDY ON CORRELATION BETWEEN MANDIBULAR SYMPHYSIS AND CRANIOFACIAL SKELETON)

  • 노상호;이기수;박영국
    • 대한치과교정학회지
    • /
    • 제27권1호
    • /
    • pp.119-127
    • /
    • 1997
  • 안모의 수직적 부조화에 부합되는 하악이부 형태에 관하여 보고되어 왔다. 이연구는 하악이부의 형태적 변이와 두개안면골격의 수직적 변이사이에 상관성을 관찰하기 위하여 시행되었다. 성인 남녀 부정 교합자 212명의 측모두부 방사선사진에서 이부의 후경과 고경을 계측하고 이부비를 산출한 후, 이부비 평균 -1 표준편차에 포함되는 즉모군(low symphysis군)과 이부비 평균 +1 표준편차에 포함되는 측모군(high symphysis군)으로 분류하고, 이들의 측모두부방사선사진을 계측하고 비교 분석하여 다음의 결론을 얻었다. 1. 이부비가 크면 hyperdivergent face 이고, 이부비가 작으면 hypodivergent face 가 되는 경향을 보였다. 2. 이부비가 크면 하악각도 컷으며, 이들사이의 상관성은 높았다. 3. 이부비와 수직안면 고경사이에 중등도의 상관성이 있었으며, 특히 이부 후경과 전안면고경사이 및 이부 고경과 후 안면 고경사이에도 중등도의 상관성이 있었다. 4. 이부의 형태적 변이와 설골의 위치사이에는 상관성이 없었다.

  • PDF

양극산화 아크방전 처리한 티타늄 임플란트의 불소방출 특성 (Characteristics of Fluoride Releasing of Anodized Titanium Implant)

  • 김하영;송광엽;배태성
    • 구강회복응용과학지
    • /
    • 제24권4호
    • /
    • pp.361-369
    • /
    • 2008
  • 본 연구의 목적은 다공성의 티타늄 산화막에 불소를 처리한 결과를 평가하는 것이다. 디스크에 양극산화법을 통하여 다공성의 티타늄산화막표면을 얻은 후 불소를 처리하고 일정시간동안 Hank액에 침전을 시켰다. 양극산화를 통해 일정거칠기의 표면을 얻었으며 SEM과 XRD를 통하여 표면의 형상과 성분을 분석하였다. 생성된 표면은 빠른 표면 활성도를 보였으며, 적절한 거칠기와 좋은 골반응으로 골유착에 기여할 수 있을 것으로 여겨진다.

저신장과 비만도의 상관성 분석 - 저신장을 주소로 내원한 환아 236명을 대상으로 (Correlation between Short Stature and Obese Degree - Intended for the Case of 236 Patient in the Name of Short Stature)

  • 이경희;송재철;이재성;곽창규;박선영;한승무;임사비나;신현택
    • 한방비만학회지
    • /
    • 제5권1호
    • /
    • pp.97-107
    • /
    • 2005
  • Objectives The purpose of this study was to investigate correlation between short stature and obese degree. And we also wanted to know the characteristics of patients visited clinic in the name of 'short stature'. Methods Height, body weight, BMI, fat mass, lean body mass, percent body fat were taken intended for 236 patients in the name of short stature. To all the patient questionnaire for growth clinic was drawn up, and ultrasound scan was taken through calcaneous of the right foot. The patients were classified to three groups - short, average, tall group - according to relative position of mid-parental height. It was analysed that the differences between groups in obese degree. Also was investigated correlation between position of short stature and obese degree, and between obese degree and bony maturity. Results & Conclusion 1. The average ages of patients in the name of 'short stature' were $12.69{\pm}3.93$ years old in boys, $10.66{\pm}3.67$ years old in girls. And it seemed to be just before second rapid maturing period. 2. The average BMI were $20.58{\pm}4.07kg/m^2$ in boys, $18.65{\pm}2.85kg/m^2$ in girls, and average percent body fat were $21.99{\pm}7.35%$ in boys, $26.01{\pm}6.35%$ in girls. 3. The numbers of obese children were 34(31.2%) in boys, 19(14.9%) in girls on the basis of BMI. And the numbers were 39(35.8%) in boys, 53(41.7%) in girls on the basis of percent body fat. There was a big difference in case of girls. 4. The numbers of AG(average group) were 48(44.0%) in boys, 60(47.2%) in girls, the numbers of SG(short group) were 35(32.1%) in boys, 31(24.4%) in girls, and numbers of TG(tall group) were 26(23.9%) in boys, 35(27.6%) in girls. 5. There were no significant differences among the groups in BMI, lean body mass, fat mass, percent body fat. Only significant difference in DI(disease index). 6. There were no significant correlation between PH(percent height) and BMI, leanbody mass, fat mass, percent body fat. Only significant correlation in DI(disease index). 7. There were no significant differences between DA(difference between bone age and chronorogical age) and BMI, leanbody mass, percent body fat. Only significant correlation in fat mass.

  • PDF

한방의료이용 행태와 이에 영향을 미치는 요인 분석 (Analysis of Factors Influencing Behavior of Oriental Medicine Utilization)

  • 김성진;남철현;김재돈;김병화;김기열
    • 대한예방한의학회지
    • /
    • 제8권1호
    • /
    • pp.89-107
    • /
    • 2004
  • This study was conducted to analyze community residents' behavior of Oriental medicine utilization and its related factors in order to provide basic data for formulation of policies on Oriental medicine. The subjects of this study was 500 residents who lived in big or medium sized cities and towns or villages Data were collected from March, 2002 to June, 2002. The results of this study can be summarized as follows. 1) According to socio-demographic characteristics of the respondents, female was 50.3%; 'over 50 years old' 29.9%, 'over college graduate' 39.7%, 'housewife' 23.0%, 'having spouse' 62.1%, 'Buddhist' 50.8%, 'living in big cities' 59.0%, 'middle economic class' 88.1%. 2) The highest proportion of frequency of Oriental medicine utilization was over 10 times(32.5%). The respondents visited Oriental medicine institutions for taking invigorant(51.1%), treatment of diseases in muscle or bone system(30.8%), treatment of diseases in digestive system(6.3%), etc. 3) According to the reasons of utilizing Oriental medicine, the proportion of good effect was highest(36.3%). 66.8% of the respondents replied that Oriental medical fee was expensive, while 0.8% of them replied that it was not expensive. 33.3% of them thought it was proper. 4) 35.5% of the respondents replied that treatment by Oriental medicine could cause side effect and 40.3% of them replied that the side effect could be caused by taking herb medicine. 5) 62.8% of the respondents replied that they would continuously receive opinions on Oriental medicine. The score of knowledge level of treatment by Oriental medicine $6.25{\pm}2.82$ points on the basis of 14 points. 6) The variables significantly influencing utilization of Oriental medicine includes taking diseases, living in big cities, male, upper (economic class, having religion, and effect of Oriental medicine. 7) The factors affecting effect of herb medicine were effect of treatment by Oriental medicine, marital status, knowledge level of Oriental medicine, having diseases, and frequency of receiving the treatment.

  • PDF

17β-estradiol mediated effects on pluripotency transcription factors and differentiation capacity in mesenchymal stem cells derived porcine from newborns as steroid hormones non-functional donors

  • Lee, Won-Jae;Park, Ji-Sung;Lee, HyeonJeong;Lee, Seung-Chan;Lee, Jeong-Hyun;Ock, Sun-A;Rho, Gyu-Jin;Lee, Sung-Lim
    • 한국수정란이식학회지
    • /
    • 제32권3호
    • /
    • pp.209-220
    • /
    • 2017
  • The estrogen-mediated effect of mesenchymal stem cells (MSCs) is a highly critical factor for the clinical application of MSCs. However, the present study is conducted on MSCs derived from adult donors, which have different physiological status with steroid hormonal changes. Therefore, we explores the important role of $17{\beta}$-estradiol (E2) in MSCs derived from female and male newborn piglets (NF- and NM-pBMSCs), which are non-sexually matured donors with steroid hormones. The results revealed that in vitro treatment of MSCs with E2 improved cell proliferation, but the rates varied according to the gender of the newborn donors. Following in vitro treatment of newborn MSCs with E2, mRNA levels of Oct3/4 and Sox2 increased in both genders of MSCs and they may be correlated with both estrogen receptor ${\alpha}$ ($ER{\alpha}$) and $ER{\beta}$ in NF-pBMSCs, but NM-pBMSCs were only correlated with $ER{\alpha}$. Moreover, E2-treated NF-pBMSCs decreased in ${\beta}$-galactosidase activity but no influence on NM-pBMSCs. In E2-mediated differentiation capacity, E2 induced an increase in the osteogenic and chondrogenic abilities of both pBMSCs, but adipogenic ability may increased only in NF-pBMSCs. These results demonstrate that E2 could affect both genders of newborn donor-derived MSCs, but the regulatory role of E2 varies depending on gender-dependent characteristics even though the original newborn donors had not been affected by functional steroid hormones.

전라도 농촌장수지역 거주 70대 노인의 건강상태에 영향을 미치는 환경적 요인에 대한 탐색 연구 (Investigation on Influencing Environmental Factors on Health Status of Korean Septuagenarians Dwelling in Longevity Region in Jeonla Province)

  • 곽충실;연미영;이미숙;오세인;박상철
    • 대한지역사회영양학회지
    • /
    • 제19권2호
    • /
    • pp.142-162
    • /
    • 2014
  • Objectives: To evaluate the critical environmental factors on healthy-aging of Korean people, we investigated the significant factors influencing health status of septuagenarians living in rural area of Jeonla province, known to be one of the representative longevity regions in Korea. Methods: We divided subjects into healthy group (36M/25F) or poor-health group (26M/73F) based on self-reported health status, body mass index, a number of prescription, and blood test data. General characteristics, physical measurements, lifestyle, dietary behavior and nutrient intake, physical health and mental health data were statistically compared between the two groups. Results: Average age was not different between healthy group and poor-health group in men and women, respectively. In men, significantly favorable factors to health were observed to be higher education, regular exercise, higher grip strength and walking function, body mass index (${\geq}18.5kg/m^2$), moderate frequency of drinking and eating-out, non-smoking, normal red blood cell (RBC) count, higher serum dehydroepiandrosterone-sulfate (DHEAS) level, good digestive function and appetite, normal hearing function, regular meals, adequate vegetable and fruit intake, diverse food intake, adequate energy and nutrients (protein, vitamin $B_1$, $B_6$, C and E, folate, niacin, P, Zn and K) intake, higher mini-nutrient status assessment (MNA) score and low level of depression. On the other hand, in women, those were literacy, living arrangement, moderate frequency of drinking, healthy teeth, higher grip strength and walking function, bone mineral density, normal RBC and white blood cell (WBC) count, higher DHEAS concentration, higher MNA score, normal cognition and memory function, having snack and adequate fruit intake. Conclusions: These results could be useful to plan effective strategies to increase health-life expectancy of Korean old people living in rural areas.

효율적인 공사수행을 위한 공사이행보증제도 개선방안 연구 (A Study on Improving Performance Bond System for Efficient Execution of Public Construction Works)

  • 김명수
    • 한국건설관리학회논문집
    • /
    • 제21권4호
    • /
    • pp.21-29
    • /
    • 2020
  • 본 연구에서는 사례분석 및 전문가 조사를 통하여 공사이행보증제도 운영의 문제점을 적시하고 개선방안을 제시하였다. 국가계약법에서는 공사계약에 있어서 계약상대자가 계약상의 의무를 이행하지 못하는 경우 계약상대자를 대신하여 계약상의 의무를 이행할 것을 보증하되, 이를 보증한 기관이 의무를 이행하지 아니하는 경우에는 일정금액을 납부할 것을 보증하는 공사이행보증서 제출을 의무화하고 있다. 현행 정부계약제도는 공사이행보증의 보증사고 시 잔여공사의 규모, 기술적 난이도, 특수공정의 포함 여부 등을 고려하지 않고 보증이행업체에게 일률적으로 당초 입찰공고 공고에서의 입찰참가자격을 요구하고 있다. 그리고 공동도급에서 보증사고가 발생했을 때, 잔존구성원에게 엄격하게 전체 계약에 대한 계약이행요건을 요구하여 많은 문제가 발생하고 있다. 본 연구에서 제시한 개선방안은 다음과 같다. 먼저, 보증이행업체 선정에서 잔여공사의 현황을 고려하여 예외적으로 보증이행업체의 자격을 완화해야 한다. 둘째, 공동도급의 경우 잔존 구성원의 계약이행요건을 '해당 계약이행요건'에서 '잔여공사의 계약이행요건'으로 완화시켜야 한다. 셋째, 보증이행공사의 하자담보책임을 원사업자 또는 보증기관이 부담하도록 공사이행보증의 채무범위에 하자담보채무가 포함되도록 개선해야 한다.