• Title/Summary/Keyword: Bombyx mori L

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Studies on Artificial Hatching of Hibernating Eggs, Bombyx mori L. (월년잠종의 인공부화에 관한 연구)

  • 김윤식;손해용
    • Journal of Sericultural and Entomological Science
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    • v.18 no.1
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    • pp.7-10
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    • 1976
  • This experiments were carried out to know hatching power on treatments of cold temperature and hydrochioric acid Iron Late January to Early February. Used silkworm variety was Jam 103${\times}$ Jam 104 laying in Spring. The results may be summarized as follows: 1. The resting of silkworm eggs activated to Late January, to longer cold treatment was, the more effects of activating increased completely. So activated eggs were possible to incubation. 2. How to the light or short hours of hycrochloric acid were compare with heavy or long each other, it was inclined that the former seems to be worse than the latter. 3. On the interaction of cold treatments and hydrochloric acid, in case of short cold treatment, hatching power was better effect all treatments of hydrochloric acid than control, but no difference between specific gravity or treatment hours. On tile contrary, in case of more longer of cold treatments, hatching power depended not only upon the specific gravity, but also upon the treatment hours.

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Effects of Insect Growth Regulators on Developmental Physiology of the Silkworm, Bombyx mori L. III. With reference to the influence of Fenoxycarb on the egg laying and the hatching (곤충 성장조절제(IGR)가 누에의 발육생리에 미치는 영향 III. Fenoxycarb가 산란 및 부화에 미치는 영향)

  • 황석조
    • Journal of Sericultural and Entomological Science
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    • v.39 no.1
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    • pp.17-21
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    • 1997
  • Oral application of fenoxycarb, O-ethyl N-(2-(4-phenoxyphenoxy)ethyl) carbamate, the commercial formulation Insegar, on the 1st day of the 3rd instar made no difference in the egg laying and the hatchability as compared to 750 grains for the number of eggs laid, 53 grains for the number of the resideual eggs unlaid, 749 grains for the number of fertirized eggs, 97% for the total hatchability, and 95% for the useful hatchability as in the control but on the first day of the 4th instar, the number of the remainder eggs in the ovary increased by 2.4 folds, and reduced number of the eggs laid, number of unfertilized eggs, the hatchabillity and useful hatchability by 39%, by 66%, by 68% and by 72%, respectively. When diluted Insegar was applied topically every other two days from the pupae to the moth at concentration of 10-6, 10-7, 10-8, 10-9, 10-10 the number of eggs laid, the number of the unfertilized eggs, the total hatchability and the useful hatchability were decreased. More severe decrease took place as concentration lowered and the earlier application.

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Quantitative analysis of rutin content using silkworm genetic resources

  • Ju, Wan-Taek;Kim, Kee-Young;Sung, Gyoo-Byung;Kim, Yong-Soon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.31 no.2
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    • pp.56-61
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    • 2015
  • Rutin is an important bioflavonoid that is consumed in the daily diet. This study compared the functional components of rutin from various silkworm species using a gene database with those of rutin produced by silkworms selectively bred through cross-combinations. We made comparisons between the geographical origin and species of silkworm using a gene database and discovered that rutin activity was ranked in the following order by species, Chinese (C5)> miscellaneous varieties (Jamsaeng 1 Ho) >Japanese (Jam 115) > European (E58) >Korean (Sun 3 ho). However, rutin levels with respect to various genetic traits (blood color, silk color, and egg color) were consistent. In order to study rutin changes that occurred during the cross breeding of the silkworm gene, we bred cross-combinations utilizing Jam 115 and the 4051 silkworms. In conclusion, in order to provide information about the constituents of functional materials contained in silkworm powder, it is imperative that silkworm cross breeding occurs so that the database of functional materials extracted from silkworms will expand.

Boil-off Loss Ratio of Cocoon Shell as a Selection Criterion in the Newly Developed Bivoltine Silkworm (Bombyx mori L.) Hybrids

  • Seetharamulu, J.;Seshagiri, S.V.;Raju, P.J.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.26 no.1
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    • pp.13-21
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    • 2013
  • Boil-off loss ratio is one of the most important economic traits to be considered during the course of silkworm breeding. The boil-off loss ratio varies among the breeds and seasons. The present study was undertaken with 5 oval and 5 peanut breeds and twenty five hybrids involving these parents to select the promising hybrids with desired boil-off loss ratio. Accordingly, studies were undertaken to estimate the heterosis and heterobeltiosis. It was observed that majority of the hybrids revealed negative heterosis for boil-off loss ratio which is desirable. Based on the heterosis and heterobeltiosis, among all the hybrids, the hybrid APBRO5 ${\times}$ APBRD5 exhibited highest negative heterosis for boil-off loss ratio and also exhibited highest EI value (64.5) for the quantitative and qualitative traits. Based on the hybrids performance, boil-off loss ratio, heterosis and heterobeltiosis and evaluation index, the hybrids viz. APBRO5 ${\times}$ APBRD5, APBRO1 ${\times}$ APBRD1 and APBRO3 ${\times}$ APBRD4 were identified for commercial exploitation.

Biochemical Characteristics of Spodoptera exigua Nuclear Polyhedrosis Virus (파밤나방 핵다각체병 바이러스의 생화학적 특성)

  • 진병래;박범석;재연호;강석권
    • Korean journal of applied entomology
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    • v.30 no.2
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    • pp.144-149
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    • 1991
  • Biochemical characteristics of Spodoptera uigua nuclear polyhedrosis virus (SeNPV) isolated in Jinju were studied. SeNPV contained a number of nucleocapsids within a viral envelope embeded in polyhedra. The polyhedral protein of SeNPV was composed of a single polypeptide with a M. W. of 30kd. Double-immunodiffusion test showed that the polyhedral protein of SeNPV had common antigenic determinants with SINPV and BmNPV. Virion proteins of SeNPV were resolved into 49 polypeptides by silver staining after SDS-PAGE. The approximate genome size of SeNPV by restriction endonuclease analysis was 1l0kb.

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Application of Paraformaldehyde (Neo-PPS) Fumigation to the Disinfection of the Silkworm Larvae, Bombyx mori L., for the Control of Silkworm Diseases (II). Chemical Damage of Neo-PPS to the Silkworm Larvae (Paraformaldehyde(Neo-PPS)$\circledR$훈증에 의한 잠체소독에 관한 연구 II. 약해에 관한 시험)

  • 김근영;이재창
    • Journal of Sericultural and Entomological Science
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    • v.24 no.1
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    • pp.25-27
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    • 1982
  • The use of fumigation of Neo-PPS was tested for the larval disinfection and at the same time for easy handling and effective chemicals for larval disinfection. The results obtained are as follows: 1. There was no decrease of cocoon yield in spring season due to chemical damage by application of Neo-PPS fumigation to the disinfection of the silkworm except 2,4 and 6 hours' treatment everyday and 6 hours'/ twice/instar treatment as compared with the control. 2. There was no significance in pupal percent between treatments and control in spring season except 6 hours' treatment everyday. 3. No chemical damage seemed to give rise to percentage of cocoon shell in all treatments.

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Effect of Anti-Juvenile Hormone Analogue (AJH) Treatments on the Silk Filament Properties of the Silkworm, Bombyx mori L.

  • Moe;Bae, Do-Gyu;Sohn, Hae-Ryong;Seol, Kwang-Youl
    • Journal of Sericultural and Entomological Science
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    • v.40 no.2
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    • pp.150-157
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    • 1998
  • This study was carried out to define the effects of anti-juvenile hormone analogue(AJH) treatment at different silkworm larval stages on some silk filament properties. It was revealed that the treatments at the 1st and 2nd days of the 3rd instar as well as the 1st day of the 4th instar resulted to trimolters induction without lethal effect. The trimolters induced by treatment at the 1st day of the 4th instar showed the shortest larval duration and the lowest single cocoon weight, cocoon shell ratio, cocoon size, single filament length and denier of filament compared with those of the control, while the trimolters induced by the 3rd-instar treatment showed intermediate values. All treatments showed no significant differences from the aspect fo degumming ratio, elongation rate and crystallinity index of fibroin. However the silk filament obtained from the treatment at the 1st day of the 4th instar showed the highest tenacity which was revealed to be related to the better uniformity of filament by the means of microscopic photographing. FT-IR spectroscopy of the silk fibroin showed a sharp and strong peak at the position of 794.72 cm-1 in all AJH-treated samples regardless of trimolters induction, which was absent in control.

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The Tonic Effect of the Extract from Male Silkworm (Bombyx mori L.) Pupae on Rats

  • Ryu, Kang-Sun;Ahn, Mi-Young;Lee, Heui-Sam;Kim, Iksoo;Kim, Jin-Won;Kim, Sang-Hyun;Park, Jin-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.123-126
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    • 2002
  • More than three hundred million men in the world are suffering from erectile dysfunction. Korean ancient medical records say that the unmated male silkworm moth is effective in strengthening men's vitality, but no scientific examination has preyed its clinical efficacy. Currently, silkworm moths are not allowed for food-stuff. Thus, we selected silkworm of 14-days-after-metamorphosis, at which external morphology possesses that of pupae, but internal component is that of moths. To obtain the reliable source of unmated male silkworm moth, we used the sex-limited silkworm breed with larval marking. The body marker allows casual separation of sex during larval period, preventing mating right after emergence from cocoon. Using the extract prepared with the pupae of 14-days-after-metamorphosis, we investigated the tonic effect of the extract on the rat fed the extract for three weeks. The results showed that the testosterone levels in serum increased maximum by 19%, that of testicle increased maximum by 200%, and athletic endurance of the rats rose by 6%, suggesting positive tonic effect of the pupae prepared at the stage of 14 days after metamorphosis.

Quantitative Analysis of 1-Deoxynojirimycin Content Using Silkworm Genetic Resources

  • Ju, Wan-Taek;Kim, Kee-Young;Sung, Gyoo-Byung;Kim, Yong-Soon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.29 no.2
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    • pp.162-168
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    • 2014
  • 1-Deoxynojirimycin(1-DNJ or DNJ), a component in silkworm powder, prevents glucose from being absorbed into the bloodstream from the small intestine by inhibiting ${\alpha}$-glucosidase activity. This study compared the functional components of 1-DNJ from various silkworm species using a gene database with those of 1-DNJ produced by silkworms bred through cross-combinations. We utilized comparisons of geographical origins and species of silkworms using a gene database and discovered that 1-DNJ activity was ranked in the following order by species, Japanese (SK-1) > Chinese (C48) > European (Rock191). 1-DNJ constituted varying percentages of silkworm organs in the following order, blood > epithelial tissue > body fat > silk glands. With regard to sex, 1-DNJ, levels were higher in males than females. However, 1-DNJ levels with respect to various genetic traits (blood color, silk color, and egg color) were consistent. In order to study 1-DNJ changes that occurred during cross breeding of the silkworm gene, we bred cross-combinations utilizing SK-1 and C48 silkworms. In conclusion, in order to provide information about the constituents of functional materials contained in silkworm powder, it is imperative that silkworm cross breeding occurs so that the database of functional materials extracted from silkworms will expand.

Serichlor, A New Disinfectant in Indian Sericulture

  • Balavenkatasubbaiah M.;Nataraju B.;Sharma S.D.;Selvakumar T.;Chandrasekharan K.;Rao P. Sudhakara
    • International Journal of Industrial Entomology and Biomaterials
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    • v.12 no.1
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    • pp.7-14
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    • 2006
  • Silkworm diseases are better prevented than cured. Disinfection and hygiene are the two important aspects in silkworm rearing to prevent the diseases. Suitable disinfectant is the primary need to disinfect the rearing house, its surroundings and appliances to eliminate the persistent pathogens from the rearing environment. In this direction, Serichlor, a new disinfectant in Indian Sericulture marketed as Serichlor-60 (contains 60,000 ppm of chlorine dioxide) and Serichlor-20 (contains 20,000 ppm of chlorine dioxide) has been evaluated for its germicidal effect against the pathogens of silkworm, viz., spores of Nosema bombycis, Bacillus thuringiensis, polyhedra of BmNPV and conidia of Beauveria bassiana both in vitro and in vivo. Results indicated that high concentration (2,500 ppm of chlorine dioxide) is required to kill all the pathogens at 100% level. The efficacy of the Serichlor was greatly enhanced by the addition of 0.5% slaked lime solution. 500 ppm of chlorine dioxide in 0.5% slaked lime solution was found effective against all the pathogens tested. This concentration of disinfectant was also found effective for disinfection of rearing house, rearing appliances and silkworm egg surface. The disinfectant is stable, non hazardous, least corrosive and most suitable for Indian Sericulture.